Northern blot results show that nos. 66 - 1, 84, 89 - 1, 97, 108, 152, 175 and 233 have stronger signal in sp6 - tester than in sp6 - driver ; and no. 23 has weak signal only in sp6 - tester, nos. 94, 165, 172, 185 and 191 have similar hybridization signals in both sp6 - tester and sp6 - driver ; nos. 4, 17, 18, 28, 6 9, 101, 156 - 1, 157 - 1 and 183 do not reveal hybridization signals in both sp6 - tester and sp6 - driver ; the results of sequencing and blastn and blastx on ncbi indicate that no. 23 cdna ( 846bp ) has significant alignments with nicotiana tabacum mrna for elicitor inducible beta - 1 - glucanase nt - sube76, and arabidopsis thaliana clone 7119 for glycosyl hydrolase family 17 ( protein id : at5g55180. 1, supported by cdna : 7119, supported by cdna : gi _ l 87001 54 ) and arabidopsis thaliana beta - 1 - glucanase - like protein ( gi _ 2 1594590 ) ; no. 84 cdna ( 560bp ) has significant alignment with lotus corniculatus aspartate aminotransferase mrna ( complete cds length = 1685, gi | 2605931 | gb | af029898. 1 | af029898 ) for aspartate aminotransferase ; no. 89 - 1 cdna has significant alignment with arabidopsis tha
與同源性最高的擬南芥類似晚期胚胎發生高豐度蛋白比較，二者都具有lea 2結構域、保守分泌蛋白cog5608結構域和低復雜度區，都具有pkc磷酸化位點、酪蛋白激
酶磷酸化位點、 n十四
酞化位點和酚胺化位點，所不同的是： （ ）在結構功能域上， 152全長cdna編碼的蛋白質序列中多了1個lea 2結構域、 l個保守分泌蛋白cog5608結構域和1個低復雜度區； （ 2 ）在功能位點上， 152全長cdna編碼的蛋白質具有酪氨酸硫酸化位點、多了l個酪氨酸激
酶磷酸化位點和1個可能的天冬氨酸富集區，但沒有n糖基化位點； （ 3 ）擬南芥類似晚期胚胎發生高豐度蛋白的lea 2結構域具有顯著性（ e
Purification and characterization of phytase from a. niger an 01001 a. niger an01001 was inoculated on solid media and cultivated at 30 for 5 days. proteins were extracted from solid - state fermentation with 50mm acetate buffer ( ph5. 5 ). the molecule weight of the phytase protein was determined as about 78kd by sds - page. the purification procedures include ammonium sulfate precipitation, deae - cellulose ion - exchange chromatography, gel electrophoresis and electroelution
3 .植酸
酶的分離純化及其性質研究黑麴黴ano1001經固體發酵，用緩沖液抽提后，經硫酸按沉澱， deae一纖維素離子交換層析，聚丙烯
酞胺凝膠電泳和電洗脫等純化步驟獲得的植酸
酶，用sds一page檢測為一條均一譜帶，其分子量約為78kd 。
5, histone acetyltransferase gene ygcns and histone deacetylase gene yrpd3 were cloned and expressed, which has laid down a foundation for studying chromosome remodeling in vitro
5 ，我們克隆並純化了酵母中的組蛋白乙
酞基轉移
酶基因gcns和去乙
酞化
酶基因rpd3 。
A aada2 cassette, encoding aminoglycoside adenylyltransferase, was found in 2 isolates of shigella sonnei, and a two cassettes array of dfra1, encoding dihydrofolate reductase, and aadala in one isolate of shigella sonnei, and another two cassettes array of dfra1 7 and aada5 in two isolates of shigella sonnei and shigella flexneri y - variant each, and a dfrv cassette in one isolate of shigella flexneri 6, respectively
對其可變區測序分析，其中2株宋內志賀菌i類整合子均攜帶1個基因盒，為氨基糖昔腺營
酞基轉移
酶（ aminoglycosideadenylyltransferase ） az基因（ aa朔2 ） ，編碼對鏈黴素的抗性。
Glu of cns mainly comes from glutamine and phosphate activated glutaminase ( pag ) can decomposed the glutamine to glu and ammonia and is the main source of the glu of the cns, so pag is used as the special marker of glutaminergic neurons
磷酸激活的谷氨
酞胺
酶（ pag ）能催化谷氨
酞胺水解為giu和氨，是中樞內gill的主要來源。困此pag一直被作為研究gill能神經元的特異性標識物。
Sleep / waking cycle is a complex network modulation and many factors such as interleukin - 1 ( il - 1 ), tumor necrosis factor ( tnf ), growth hormone releasing hormone ( ghrh ), vasoactive intestina polypeptide ( vip ) and many conventional neurotransmitters such as serotonin ( 5 - ht ), acetylcholine ( ach ), norepinephrine ( ne ), dopamine ( da ) and gamma - aminobutyric acid ( gaba ) were involved in it. recent evidence has shown that no synthesized in neurons in several areas of the brain can induce the release of neurotransmitters. in the rat central nervous system, the anatomical distribution of nos - containing neurons is now well established, and it was reported that nos is co - localized with neurotransmitters well known for their involvement in sleep mechanisms, i. e. 5 - ht, ach, da and gaba
鄭州大學2003屆碩士畢業論文gaba受體激動劑消除no合成
酶抑制劑對大鼠睡/醒周期的影響睡/醒周期的形成是一個復雜的網路調控的結果，體內許多因子都參與了這一調控網路，這些因子如白介素一1 （ il一1 ） 、腫瘤壞死因子（ tnf ） 、生長激素釋放激素（ ghrh ） 、血管活性腸膚（ vip ）以及經典的神經遞質如5一輕色氨（ 5一ht ） 、乙
酞膽堿（ ach ） 、去甲腎上腺素（ ne ） 、多巴胺（ da ）和卜氨基丁酸（ gaba ）等，它們在睡眠的發生和調節中也發揮著重要作用。
A screening method that allows available, efficient and reliable selection of hydantoinase - positive micro - organisms was devised. the soil samples were collected from different region, which were enriched by using 5 - phenylhydantoin as the unique nitrogen. the first detection was through screening plates, the 23 candidate strains were further selected by detection of the medial product and final product
2 .建立了一個可行的篩選模型，從不同區域採集土樣，以5一苯基乙內
酞服為唯一氮源進行土樣富集，通過平板篩選法進行初篩后，得到23株候選菌株，通過檢測中間產物和終產物的產生進行復篩，結果初步確定了1株乙內
酞脈
酶產
酶菌株，編號為bts11 。
Bootstrapped stepwise logistic regression was used to identify potential predictors of fl among 13 variables of interest [ gender, age, ethanol intake, alanine transaminase, aspartate transaminase, gamma - glutamyl - transferase ( ggt ), body mass index ( bmi ), waist circumference, sum of 4 skinfolds, glucose, insulin, triglycerides, and cholesterol ]
使用靴帶逐步邏輯回歸在13個變量[性別，年齡，酒精攝入量，丙氨酸轉移
酶，天冬氨酸轉移
酶， -谷氨
酞轉移
酶（ ggt ） ，體質指數（ bmi ） ，腰圍， 4點皮膚褶總和，血糖，胰島素，甘油三酯，膽固醇]中識別出潛在的預測因子。
2. 4 preparation of yeast telomerase, the activity of telomerase was examined by the method of trap, polyacrylamide gel electrophoresis and silver staining
2 . 4酵母端粒
酶的制備，端粒重復擴增法（ trap ） 、聚丙烯
酞胺凝膠電泳及硝酸銀染色法檢測端粒
酶活性。
The enzyme was purified from cell extract by ammonium sulfate fractionation ( 30 % - 80 % saturation ) and consecutive column chromatography using deae - cellulose and sephadex g - 100. the sod activity for purified enzyme could reach 4966 iu / mg proteins, and the molecular weight of the sod was about 20 kda which determined by sds - page electrophoresis. when the non - denaturing polyacrylamide gel stained with substrate for sod in the present of 0. 2mmol / l kcn or 0. 5 mmol / l h2o2, the active band was still showed at the same position, which indicated the sod could not be inhibited by the treatments with kcn or h2o2 and it was the mn sod
通過sds一聚丙烯酸胺凝膠電泳可知該sod
酶的分子量約為20kda .在非變性聚丙烯
酞胺凝膠（ pagb ）電泳后，在凝膠son顯色反應液中加入0 . 2耐01 / lkcn或0 . 5inmol / lh202 ，凝膠sod顯色反應后在原來的sod
酶部位仍然含有sod活性帶，這表明利用kcn和h20 :處理並不能抑制500的活性，該sod屬于mn一sod 。
Combined with the results of histone deactylase inhibitor, it is suggested that histone acetylation plays an important role in hsp gene transcription regulation
結合以上去乙
酞化
酶抑制劑的實驗結果，我們認為組蛋白乙
酞化修飾參與了熱休克基因的表達調控。
One strain possessing hydantoinase activity were isolated and named bt811. it was proved that the bt811 was a new d - hydantoinase - producing strain by gene separation and sequence analysis
后經基因分離和序列分析，證實bt8ll為1株新的d一乙內
酞脈
酶產
酶菌株。
4, compared the influence of two histone deacetylase inhibitors on the hsp70 gene transcription, after changing the time and the concentration of the inhibitors, we found the mechanism of the two histone deaceylase inhibitors is different
4 ，通過比較改變餵食時間和餵食濃度后兩種去乙酞化酶抑制劑與熱休克基因表達水平之間的關系，我們發現tsa與丁酸鹽抑制去乙酞化酶的作用機制可能是不同的。
