酰基載體蛋白 的英文怎麼說

中文拼音 [jīzǎitǐdànbái]
酰基載體蛋白 英文
acp, acyl carrier protein
  • 酰 : 名詞[化學] （酰基） acyl
  • 載 : 載Ⅰ名詞（年） year : 一年半載 six to twelve months; six months to a year; 三年五載 three to five ...
  • 體 : 體構詞成分。
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 載體 : [化學] carrier; supporter; isotopic carrier
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  1. In the experiment, the full code sequence of bar gene was cloned by pcr from transgenic herbicide resistant bobwhite wheat and checked. it was expressed in e. coli and its protein was determined. after having been properly modified, the bar gene which correctly codes pat was cloned into binary vector pbi121 and then transferred into lba4404 by triparantal crossing, which is the prerequisite work for genetic transformation

    本實驗從抗除草劑轉基因bobwhite小麥中，利用pcr克隆的方法擴增出bar基因全長，並在原核表達系統中表達，鑒定表達蛋白的活性，將能夠正確編碼ppt乙酰轉移酶的bar基因片段，經過適當的修飾構建入真核表達載體。
  2. Price, a. c. et al. " inhibition of b - ketoacyl - acyl carrier protein synthases by thiolactomycin and cerulenin. " j. biol. chem. 276 ( 2001 ) : 6551 - 9

    硫乳黴素與淺藍菌素對酮酰基酰基載體蛋白合成酶的抑制作用， 《生物化學期刊》 276 （ 2001 ） : 6551 - 9
  3. In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ), and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee. the plasmid pfb 1 - cee was transformed into dh1 obac competent e. coli cells. high molecular weight dna was prepared from the overnight cultures from the selected e. coli colonies, which was recombinant baculovirus shuttle vector containing hcv structural gene, named bac - cee

    本實驗用pcr擴增hcv結構區基因，克隆到桿狀病毒表達載體pfastbacl中，構建成重組轉座載體pfb1 - cee ，轉化dh10bac大腸桿菌感受態細胞，篩選陽性菌落，抽提大分子質粒dna ，獲得含hcv結構區基因的重組桿狀病毒穿梭載體bac - cee ，脂質體介導轉染sf9昆蟲細胞，出現細胞病變后，收集含有重組桿狀病毒顆粒的培養上消，重新感染sf9細胞，收集sf9細胞，進行12 . 5 sds -聚丙烯酰胺凝膠電泳，可見表達的蛋白條帶。
  4. 4. the expressed result of pbv - a, pbv - b and pbv - c showed that the expressed protein was soluble and no inclusion body was been found. sds - page analysis show the molecular weight of protein expressed by phaa was 42kda which was equal to 3 - ketohilase, the molecular weight of protein expressed by phab was 26kda which was equal to acetoacetyl - coa reductase, and the molecular weight of protein expressed by phac was 63kda which was equal to pha synthase

    表達載體pbv - a 、 pbv - b和pbv - c經誘導表達，發現所表達的蛋白均為可溶性蛋白，沒有包涵體出現；蛋白經sds ? page分析表明，基因phaa表達的蛋白分子量為42kda ，與?酮硫裂解酶分子量大小一致；基因phab表達的蛋白分子量為26kda ，與乙酰乙酰coa還原酶分子量大小一致；基因phac表達的蛋白分子量為63kda ，與pha合成酶分子量大小一致。
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