酵母基因轉換 的英文怎麼說

中文拼音 [jiàomǔjīyīnzhuǎnhuàn]
酵母基因轉換 英文
yeast gene conversion
  • 酵 : 動詞（發酵） ferment; leaven
  • 母 : Ⅰ名詞1 （母親） mother 2 （泛指女性長輩） one s elderly female relatives 3 （配套的兩件東西里的凹...
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  • 轉 : 轉構詞成分。
  • 換 : 動詞1. （給人東西同時從他那裡取得別的東西） exchange; barter; trade 2. （變換; 更換） change 3. （兌換） exchange; cash
  • 酵母 : yeast酵母浸液[提取液] yeast extract; 酵母聚糖 zymosan; 酵母片 aluzyme; 酵母細胞[植物] yeast plant; yeast cell
  • 轉換 : change; transform; convert; switch
  1. The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins

    將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。
  2. Cdc5 gene is necessary for the g2 / m transition. cells with mutations in cdc5 can not finish the g2 / m transition, then die ; overexpression of cdc5 can shorten g2, the cell cycle as well. it also makes cells lessening

    1976年， nurse等在篩選裂殖酵母（ s . pombe ）細胞周期進程突變體的過程中得到了基因cdc5 ，后來經實驗證明cdc5對細胞周期g2期到m期的轉換是必需的： cdc5突變后造成細胞無法完成g2 m轉換而死亡；過量表達cdc5會縮短g2期，同時縮短整個細胞周期，並導致細胞變小。
  3. The research showed that their interactions in yeast two - hybrid system are still steady when the vector was exchanged, in contrast, the interaction was disappear when the reading frame of each positive had been changed. the four positives were subcloned into suicide plasmid so that gene mutant strains could be constructed via conjugation and homologous recombination

    為了進一步驗證這些克隆的編碼產物與nifa蛋白的相互作用，將它們與nifa基因互換載體后共轉化酵母，仍然可以激活三個報告基因的表達，而陽性克隆移碼表達的重組質粒和pgbd - nifa的共轉化物則不能在選擇性平板上生長。
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