酶不活化作用 的英文怎麼說

中文拼音 [bùhuóhuàzuòyòng]
酶不活化作用 英文
enzyme inactivation
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 不 : 名詞[書面語] （剁物所用的木墩） a block of wood
  • 活 : Ⅰ動詞1 （生存; 有生命） live 2 [書面語]（救活） save （the life of a person）:活人無算 （of a goo...
  • 用 : Ⅰ動詞1 （使用） use; employ; apply 2 （多用於否定: 需要） need 3 （敬辭: 吃; 喝） eat; drink Ⅱ名...
  • 活化 : [化學] activation radioactivation; activate活化處理 activating treatment; 活化分析 [核物理] activ...
  1. Such as when the substrates was benzidine and a - naphthol, the absortion peak was 450nm after catalyticed by dna, and 501nm after catalyticed by pod, if changing substrate to benzidine and pyrocatechol, the absortion peak was 503nm after catalyticed by dna and 603nm after catalyticed by pod. the second difference was the different result after hplc : the result of hplc ( substrates : benzidine and a - naphthol ) showed that the products under the affect of dna and pod had different value on the peak and the area

    然後對以聯苯胺與-萘酚為底物，以h _ 2o _ 2為氧化劑，進行高效液相色譜分析，分析結果表明dna與過氧化物酶作用后的產物在峰面積和峰高上有不同數值；同時，不同形式的dna對該反應都有催化活性，表明只要是生物來源的dna （非特異性dna ）都具有一定程度的催化活性。
  2. Interestingly, inhibition of p38 mapk activation ( but not erk activation ) by its inhibitors or by expression of a dominant negative mutant of p38 mapk could effectively block the chemotactic effect of ( - arrestin2

    P38mapk上游激酶ask1的顯性失活突變體也可以阻斷- arrestin2對趨化的增強作用。這說明是p38mapk而不是erk信號通路與- arrestin2對趨化的增強作用相關。
  3. Inhibition by zn2 + and mn2 + additive, and almost 100 % of t he activity was inhibited under their physiologically significant concentrations. these results suggest that the protease is possibly regulated by divalent cations in vivo

    蛋白酶的活性受到某些二價金屬陽離于的抑制，比如zn訃、 mg卜、 hg熒光發射光譜結果顯示，在不同的抑制劑作用下，蛋白酶的高級構象可能發生了較大變化。
  4. Abstract : numerous mushrooms are toxic to insects. to identify the chemicals involved in insecticidal activity, the toxicity of 14 species has b een studied for water solubility, thermolability, and dialysis. the data strongly s uggest that proteins are responsible for most of the insecticidal avtivity in mu shroom fruitbodies and may be a source of genes available for plant protection a gainst insects. among proteins, lectins and haemolysins are good insecticide candi dates because the toxicities are not affected by protease

    文摘:許多蘑菇都對昆蟲表現出毒性.為了證實與殺蟲毒性有關的化合物，對14種蘑菇的毒性在水溶性、熱敏性和可透析性等方面進行了研究.研究數據表明，蛋白質對大多數蘑菇子實體的殺蟲活性起著重要作用，也許是一種可以用於植物抵抗害蟲的基因源.在數種蛋白質中，凝集素和溶血素因不受蛋白酶的影響而成為良好的殺蟲劑候選材料
  5. Numerous mushrooms are toxic to insects. to identify the chemicals involved in insecticidal activity, the toxicity of 14 species has b een studied for water solubility, thermolability, and dialysis. the data strongly s uggest that proteins are responsible for most of the insecticidal avtivity in mu shroom fruitbodies and may be a source of genes available for plant protection a gainst insects. among proteins, lectins and haemolysins are good insecticide candi dates because the toxicities are not affected by protease

    許多蘑菇都對昆蟲表現出毒性.為了證實與殺蟲毒性有關的化合物，對14種蘑菇的毒性在水溶性、熱敏性和可透析性等方面進行了研究.研究數據表明，蛋白質對大多數蘑菇子實體的殺蟲活性起著重要作用，也許是一種可以用於植物抵抗害蟲的基因源.在數種蛋白質中，凝集素和溶血素因不受蛋白酶的影響而成為良好的殺蟲劑候選材料
  6. The effort of manganese removal was studied and the kinetics of manganese removal was tried to establish. the factors of dissolved oxygen concentration, fe2 + concentration, ph, p concentration and closing of the filter were studied to evaluate their effort for biological manganese removal, and the correlation of residual manganese and oxidation - reduction potential was also discussed. as the iron content of water was high, experiment results showed that the reaction was zero order, as the iron content of water was low, the reaction was first order. the time needed for the cultivation of biological manganese removal was 60 70 days. the filter operated at the filtration rate of 8 10m / h, silica sand of effective size 0. 95 1. 25mm filled the filter to a depth of 1200mm

    試驗結果表明，成熟后濾砂表面濾膜的x射線衍射圖譜與mno _ x ? 5h _ 2o （ x = 1 . 86 ）的x射線衍射圖譜一樣，濾膜成熟后的結構在進水物質不發生變化的情況下不發生變化;合適的碳磷比對生物除錳有明顯的促進作用，試驗條件下的投磷量不會對出水造成二次污染;生物除錳需要亞鐵的參與，亞鐵的存在除了能夠促進微生物分泌胞外酶並刺激其活性外，還通過鐵離子的變價傳遞電子，催化錳離子的氧化反應，從而促進對二價錳的降解。
  7. The results indicated : ( 1 ) during the jointing stage of winter wheat, the differences of catalase activity among different measures of soil water utilization are significant in shallow soil, in which the catalase activity of furrow planting with straw mulch is the highest and that of row - spacing - reduced and close planting is the lowest ; ( 2 ) the trends of catalase activity versus depth are almost the same for different treatments, looking like an inverse s ; ( 3 ) for furrow growing without mulch, the activity of soil catalase in the furrow is higher than that in the ridge ; ( 4 ) soil catalase activity reflects some effects from different techniques of soil water utilization and may be used as a indicator for micro - eco - environment in the fields

    初步分析得出： （ 1 ）冬小麥拔節期，採用不同土壤水利用技術的冬小麥田淺層土壤過氧化氫酶活性差異明顯，以溝播不蓋秸稈處理的過氧化氫酶活性最強，縮行密植處理的最弱； （ 2 ）土壤過氧化氫酶活性隨深度的變化趨勢大體一致，呈反s形； （ 3 ）溝播不蓋秸稈處理溝中土壤過氧化氫酶活性明顯高於壟上； （ 4 ）土壤過氧化氫酶對於田間微生態環境具有指示作用，在一定程度上反映了土壤水利用技術對微生態環境的影響。
  8. The a - transglucosidase was selectively modified by pcmb, me, edc, clac, acetyl acetone and nbs, and changes in the activities of the enzyme have been detected. the reaction of a - transglucosidase with pcmb, me, edc and clac resulted in a strong inhibition of the enzyme activities which decreased with the increase of modifier concentration. the acetyl acetone and nbs were found without inhibition effect

    酚丙酮、小涅代唬拍酷亞胺剛b引等化學修飾劑對a葡萄糖轉苦酶的幾種氨基酸殘基進行選擇修飾，並測定其酶活力變化，結果表明： pcmb 、 me 、 edc 、 ciac能顯著抑制酶的活性，活力的下降與修飾劑的濃度有關，乙酚丙酮、 nbs白巾飾對酶的奪製作用不明顯。
  9. The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides

    進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。
  10. There was no difference in other biologic characteristic of mscs between the two separation method, such as cell anchorage ratio and clone formation ratio. ( 2 ) plga film presented uniformity frame with no protuberance and fissure under scanning electron microscopy ( sem ). big aperture with smooth wall and average 400 m i n size running - through each other was observed in porous plga substrate, around the big aperture there were many round micropores about 5 m size. all of the structure were equal and uniform, which satisfied the further research work. ( 3 ) mscs adhesion at earlier time was promoted by biotiegenrafter 3h, cell number was ( 1. 5 0. 18 ) 105 in the plga film coated with biotiegen group, which was significantly higher than that in plga film group ( p < 0. 01 ) and higher than that in coverslip group ( p < 0. 05 ), which cell number was ( 1. 04 0. 21 ) 105. after 6h and 12h biotiegen could not promote cell adhesion, and cell proliferation and alkaline phosphatase ( alp ) activity were not promoted dramatically during 9 days. ( 4 ) cell adhesion was promoted by fibronectin or collagen type i

    G ） i型膠原、纖維粘連蛋白促進細胞增殖，細胞接種后3 、 6 、 gd三個檢測時間點，實驗組細胞均明顯高於對照組。與1型膠原相比，纖維粘連蛋白刺激作用更強。 ） i型膠原、纖維粘連蛋白尚能誘導mscs細胞向成骨細胞分化，不僅表達成骨細胞標志物ocn 、 alp 、 opnmrna ，而且堿性磷酸酶活性明顯增高，堿性磷酸酶及鈣結節7第四軍醫大學博士學位論文一染色均強陽性， i型膠原組mscs細胞堿性磷酸酶活性較fn組更高，有顯著性差異；同時，兔疫組化染色表明，經纖維粘連蛋白作用的mscs1型膠原表達陽性。
  11. The variety of the activity of na + / k + - atpase, ca2 + - atpase and sod by increase calcium concentration in the water, and the effects of cd2 +, estradiol - 17 and ca2 + on the lymphocyte proliferation from blood and head kidney of ctenopharyngodon idellus were also investigated the significance of this paper is that to research the toxicity of cadmium to the fish and to search the mechanism of the cadmium toxicity to the fish. and the protection of calcium on cd2 + toxicity

    本論文研究了鎘對草魚的毒性作用，比較了在不同鈣濃度條件下，其鰓、肝臟、腦等組織中sod 、 na ~ + k ~ + ? atp酶、 ca ~ （ 2 + ） ? atp酶的活性變化規律以及鎘和17 ?雌二醇對草魚血液及頭腎淋巴細胞增殖的影響，評價鎘對魚類的毒害作用，探索鎘的毒害機理。
  12. 5 statistic treatment with spss soft. results after treatment with mms at various ( 0. 01 ~ immol / l ) concentration for 72h, we examined s. cerevisiae s288c cells for dna - damage situation and changes in telomerase activity

    5統計學處理結果將不同濃度的mms （ 0 . 01一1 ~ fl ）作用於釀酒酵母5288c細胞72h后，分別檢測其dna損傷程度及端粒酶活性變化。
  13. Different from other angiotensin receptor antagonist, telmisartan has the effect of modulating peroxisome proliferator - activated receptors, and might slightly superior to other anti - hypertensive drugs in the treatment of hypertension

    與其他沙坦類藥物不同，替米沙坦尚有激活過氧化物酶體增殖激活受體的作用，治療中可能略優于其他降壓藥物。
  14. ( 2 ) compared to secondary natural forest, the amount of three main types of microbes in rhizosphere soil of different age of larix olgensis plantation correspondingly decreased largely, enzyme activity in non - rhizosphere soil reduced, the biochemical activities abated, and soil physical and chemical properties deteriorated

    （ 2 ）與天然次生林相比，落葉松人工林不同發育階段根際土壤三大類微生物數量均相應地有較大幅度的降低，非根際土壤酶活性減弱，土壤生化作用強度降低，土壤理化性質變壞。
  15. One is a combination of ion exchange chromatography on q sepharose ff, hydrophobic interaction chromatography on phenyl hp, gel filtration chromatography on sephadex 200 and higher resolution ion exchange chromatography on mono q. the other is a combination of ion exchange chromatography on q sepharose ff, two affinity chromatography on con a sepharose 4b and benzamidine sepharose 6bcl sequentially

    上清液中重組蝗蛇毒類激血酶的純化是通過設計的不同分離純化方法組合進行的，並對每一種組合進行了活力與純度及口收率的評價，結果表明兩種組合方式都具有一定的實用性和可操作性。
  16. The decrease of organic matter and the increase of alkali - hydrolyzable nitrogen of the hydrogen treatment were higher than the control, and urease activity, nitrogen fixation arnmonification had the same tendency, and amino acids had also changed. hi addition, comparing the control, ph of hydrogen treatment was increase and eh was decrease, and microbe mass was also too

    與對照相比，氫處理培養有機質減少量小、堿解氮的增量高，脲酶活性、固氮能力、氨化作用都較強，並且不同種類的氨基酸也表現出各自相應的差異來。此外，土壤經氫處理后，土壤的ph上升， eh下降，土壤微生物生物量也呈減少趨勢。
  17. The fluorescence intensity of the phosph atase increased, followed by a decreasing process, in the temperature region of 40 - 90 c. the enzyme was inhibited by naf significantly and the fluorescence spectra of the protein were influenced obviously by the concentration of naf. the data from our experiments showed that the bivalent cations can modulate the activity of the phosphatase but maybe is independent of the tertiary structure

    Edta對此種磷酸酣酶也有抑制作用，因此用7種二價陽離子處理磷酸酷酶，發現不同金屬二價陽離子對這種磷酸酷酶活性和熒光發射光譜的影響不同，因此二價陽離子調控此酶活性，但可能與熒光光譜所反映的三級結構變化無關。
  18. The optimum ph for enzymatic reaction is 7 - 12. and the enzyme is sensitive to temperature. the fibrinolysis activity of nattokinase was determined and analyzed in the thrombolysis experiment in vitro

    選用纖維蛋白平板法作為檢測酶溶纖活性的方法，進行了一系列的理化性質測定，結果表明：酶水溶液對溫度敏感，不宜常溫保存。
  19. We know that the mouth is glands, they kept secreted in saliva, especially in the daytime by chewing, speaking of oral action, functional active glands, saliva secretion of large amount, which is in addition to saliva digestive function, due to its inhibition contain a lot of bacteria lysozyme, oral a self - cleaning and scouring role, which is very important oral self - protection

    我們知道，口腔內有腺體，它們在不停地分泌唾液，尤其在白天通過咀嚼、說話等口腔動作，使腺體功能活躍，唾液的分泌量也大，這些唾液除具有消化功能外，由於其內含有大量抑制細菌的溶菌酶，對口腔有沖刷和自潔作用，這也是口腔很重要的自我保護作用。
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