酶先 的英文怎麼說

中文拼音 [xiān]
酶先 英文
enzyme precursor
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 先 : 名詞1 （時間或次序在前的） earlier; before; first; in advance 2 （祖先; 上代） elder generation; ...
  1. Our previous studies showed existence of apoplast cam in the plant cell and cam had many extracellular functions. so it supposed cam may be one of important extracellular polypeptides and trigger the intracellular signal transduction by binding the receptor. in this study, radiolabelled ligand is used to investigate the binding characteristic of cam and a. thaliana protoplasts. and chemical crosslinking is employed to explore binding proteins in the membrane. at first, ( 35 ) ~ s - cam was produced using ( 35 ) ~ s - labeled amino acid mixture in e. coli. sds - page and autoradiograph indicated high - purified, high - specific radioactivity ( 35 ) ~ s - cam was obtained. electrophoresis of ( 35 ) ~ s - cam is the same as that of unlabeled cam with ca ~ ( 2 + ) or egta ; a quatitive of protoplasts was prepared by enzymolysis

    首先，用~ （ 35 ） s標記的氨基酸混合物喂養工程菌成功地制備了~ （ 35 ） s標記的擬南芥鈣調素（ ~ （ 35 ） s - cam ） ， ~ （ 35 ） s - cam純度高、放射活度高、 ca ~ （ 2 + ）與egta存在時的電泳行為與未標記cam相同，可作為一種高靈敏性的探針用於進行受體學分析實驗；用擬南芥種子誘導愈傷，通過酶解制備了大量原生質體。
  2. In this study, the stem segments of new shoot with axillary buds of well - growth tetraploid black locust trees were used as explants. the effects of different basic mediums, different hormone kinds and their concentrations ratios, different sucrose concentrations on calli induction, buds differentiation and rooting in the process of establishment of high frequency regeneration system of tetraploid black locust were studied. on the base of high frequency regeneration system, the effects of various factors on transformation efficiency of badh mediated by agrobacterium tumefaciens were discussed in the light of gus histochemical assays

    本實驗首先以生長良好的四倍體刺槐優株上當年生新梢的帶腋芽莖段為外植體，研究了在四倍體刺槐高頻再生體系的建立過程中不同基本培養基、不同激素濃度及其配比、不同蔗糖濃度對愈傷組織的誘導、芽的分化及生根的影響；然後在得到高頻再生體系的基礎上，通過農桿菌介導法轉化甜菜堿醛脫氫酶（ badh ）基因，以gus染色組織分析為依據探討了影響轉化效率的各種因素，建立了高效、可重復的基因轉化體系，為四倍體刺槐目的基因的導入打下了基礎。
  3. Large dna molecules are first dissected with restriction enzymes to produce specific fragments.

    首先用限制性內切酶將大的DNA分子切斷，產生出特殊的片段。
  4. In the first trial, combination of enzymatic digestion was used to prepare suspensions of spermatogenic cells from adult mouse testis, and then a modified discontinuous percoll gradient centrifugation method ( 15 %, 22 %, 30 %, 40 %, 50 %, 60 % ) was introduced to isolate spermatids from the cellular suspensions. the content of spermatids in each isolated fraction by percoll method was determined by morphology ( wright - giemsa stain ) and flow cytometry analysis, and the viability of spermatogenic cells was assessed by using eosin y exclusion test

    在第一部分試驗中，首先利用連續3次組合酶消化成年小鼠睪丸制備睪丸細胞懸液，然後經6層非連續percoll梯度離心法（ 15 、 22 、 30 、 40 、 50和60 ）分離，通過形態學和流式細胞術鑒定南京醫科大學碩士學位論文各個percoll組分中精子細胞的含量，並以伊紅y排斥試驗測定細胞的存活率。
  5. Second, the activities of protective enzymes i. e. superoxide dismutase ( sod ), peroxidase ( pod ), and ascrobate peroxidase ( apx ) exhibited down - up - down trends when treated with 2 @ and 8 @, and these activities sustained at a relatively high level. this indicated euonymus japonicus " zhuzi " had relatively high adaptability to cold stress

    （ 2 ） 『粗枝』大葉黃楊在零上低溫時，細胞保護酶超氧化物歧化酶（ sod ） 、過氧化物酶（ pod ） 、抗壞血酸過氧化物酶（ apx ）的活性先降低後上升，又下降並維持較高的水平，表明『粗枝』大葉黃楊對低溫脅迫有一定的適應性。
  6. Niger with phytase activity about 2250 u / ml which selected by protoplast - uv mutation was used as original, prepared it into fungu - suspend - liquid, through uv mutation, daub on the filter - substract. pre - primary - selection was according to the lucency - circle, primary - selection was one fungus inoculate one flask to ferment, secondary - selection was using several high phytase activity fungus through one fungus inoculate 2 - 3 flasks to ferment. then one or two high phytase activity fungus of the secondary selection were used in the next mutation cycle

    首先用粗略制備的原生質體經紫外誘變篩選到一株酶活為2250u ml的實驗出發菌；制備成菌懸液，紫外燈下照射誘變，紅光下塗抹篩選平板，恆溫培養2 3d ；按透明圈大小進行預初篩，挑選徑圈比大的菌落接斜面，恆溫培養3d ；按一株一瓶的方式進行初篩；從中選取酶活較大的3 5株，按一株2 3瓶的方式進行復篩；挑取酶活大且穩定的1 2株進入下一代誘變篩選。
  7. First, three isozymic systems ( lactate dehydrogenase ( ldh ), esteraes ( est ), malate dehydrogenase ( mdh ) ) extracted from threepopulations of mandarinfishes were detected by discontinuous vertical plate polyacrylamide gel electrophoresis ( page ). several loci were tested but none polymorphic locus was detected in qiupu river population. the results showed that : ldh can be used as biochemical markers to identify these three populations of mandarinfishes

    首先，本試驗採用聚丙烯酰胺凝膠電泳（ page ）對我省三個水域鱖魚群體3種同工酶（ ldh 、 est 、 mdh ）的不同基因座位進行了檢測，結果表明： ldh同工酶可以作為區分秋浦河鱖魚、長江鱖魚兩個群體與萬佛湖鱖魚群體的生化遺傳標記；其次，運用rapd技術分析了三群體鱖魚的基因組dna的多態性。
  8. With the increase of concentration of aqueous extract from peganum multisectum increased, root vigor, the contents of chlorophyll, soluble protein and nucleic acid in roots and shoots of alfalfa seedlings decreased, while the activities of protease and nuclease, the contents of o2 ( superscript - ), h2o2 and malondiadehyde ( mda ) increased, the activities of superoxide dismutase ( sod ), catalase ( cat ) and peroxidase ( pod ) first increased and then decreased

    幼苗根系活力和葉綠素、可溶性蛋白質、核酸含量隨水浸液濃度的提高而降低，蛋白酶和核糖核酸酶活性及超氧陰離子（ o2 （上標- ） ） 、 h2o2和丙二醛（ mda ）含量則增加，超氧化物歧化酶（ sod ） 、過氧化氫酶（ cat ）和過氧化物酶（ pod ）活性呈先升后降變化。
  9. Phenylketonuria ( pku ) is an inherited metabolic disease that results in mental retardation and other neurological problems when treatment is not started within the first few weeks of life. the disease arises from the deficiency of a single enzyme, phenylalanine hydroxylase, which converts the essential amino acid, phenylalanine, to another amino acid, tyrosine. failure of the conversion to take place results in a buildup of phenylalanine in the body that then damages the central nervous system

    苯丙酮尿癥（ pku ）是一種智力發育不全的先天性疾病，患者由於肝贓內苯丙氨酸羥化酶缺乏，苯丙氨酸不能正常代謝為酪氨酸，從而導致苯丙氨酸在肌體組織內積累，引起腦損傷和累進性精神障礙，臨床表現為智力低下，頭發顏色轉黃，尿有異臭味，重者似鼠臭。
  10. The amino group becomes attached to the coenzyme to form pyridoxamine phosphate, and is then transferred to the a - keto acid, which is usually pyruvic acid, oxaloacetic acid, or - ketoglutaric acid

    反應中氨基首先連接在輔酶上形成磷酸吡哆氨，然後氨基轉移到-酮酸上，這種-酮酸可以為丙酮酸、草酰乙酸或是-酮戊二酸。
  11. Pcr amplification using 2 degenerate primers for nitrogenase fe protein gene was performed with chromosomal dna isolated from the 29 isolates. the result suggested that a nifh amplicon of 323 nucleotides was detected in 7 isolates and the 7 isolates are c4 c5 g1 g2, w5 t1 and t7. these pcr amplified fragments were cloned, and sequenced

    首先利用芽孢桿菌中芽孢的抗熱性將土壤溶液在100沸水中煮10 - 15分鐘，然後用選擇性無氮培養基進行初篩得到29株菌落形態不同的菌株；接著用固氮酶結構基因nifh的特異性引物對這29株菌進行pcr擴增，結果表明其中7個菌株具有nifh基因，這7個菌株的編號依次為： c4 、 c5 、 g1 、 g2 、 w5 、 t1和t7 。
  12. The secret that there are scientifically approved, double blind tested methods which can enable you to lose weight in the form of fat primarily and at the same time produce positive growth in your other body components : bone, muscle, cardio respiratory organs and fluids ( blood and enzymes ) which in turn increases your overall health, immune system, and fitness

    秘籍是：科學方法所認可的兩組人群測試方法，起先能使你以（減少）脂肪的形式減輕體重，同時使你身體的其他成分：骨骼、肌肉、重要器官及黏液（血液及酶）良好生長，依次增強你的全面健康、免疫系統及健身。
  13. Northern blot hybridization showed that leacbf has well - distributed expression in different tissues of wild tomato ( pink stage ). treatment the seedling with uv made the enzyme activity of the pal increased gradually and then decreased

    用不同的紫外線照射時間處理番茄幼芽，苯丙氨酸解氨酶（ pal ）的活性呈先是增加，達到最大值后再減小的趨勢。
  14. Chapter 4 comprises two part. part reports on the manufacture and characterization of glucose oxidase - silver sol - polyvinyl butyral modified platinum electrodes with naphthol green b as an electron transfer mediator in the glucose solution

    在第四章中，首先研究了納米ag在以萘酚綠b為電子媒介體的葡萄糖氧化酶生物傳感器中的應用。
  15. In this method, high fidelity dna polymerase ( pyrobesf * dna polymerase ) was used to ensure efficient and accurate amplification. after cloning and sequencing of the pcr products, one concatemer contains seventeen copies of grb ~ ast7 was attained. the grb - ast ? concatemer was cloned into pet22b expression vector

    先用t4dnapolymerase構建成模板，再通過高保真dna聚合酶（ pyrobesttmdnapolymerase ） pcr進行擴增，從中得到一個含有17個拷貝的grb - ast _ 7的串聯體。
  16. Then enzyme was purified with a deae - cellulose ( 5. 5x50cm ) column, a toyopearl hw - 65 ( 5. 5 x 50cm ) column and a sephadex g - 200 ( 5. 5 x 80cm ) column. finally, the enzyme was purified for 10 folds with the recovery of 17. 4 %. page showed a single band for the purified creatinase

    3 、肌酸水解酶的提純酶在硫酸銨飽和度為40 80之間完全沉澱，先後經過deae - cellulose離子層析柱、 toyopearlhw - 65疏水層析柱、 sephadexg - 200分子篩層析柱層析，最終使酶提純10倍，最終得率為17 . 4 。
  17. With the increace of zn stress, the content of chlorophyll, superoxide dismutase ( sod ) activity and soluble protein content dropped significantly, peroxidase ( pod ) and catalase ( cat ) activities rised at first and lowered later, and malonyl dialdehyde ( mda ) content increased drastically

    隨鋅脅迫程度的增大，葉綠素含量、 sod （超氧化物歧化酶）活性和可溶性蛋白含量呈下降趨勢；而pod （過氧化物酶）和cat （過氧化氫酶）活性則先升后降； mda （丙二醛）含量上升。
  18. Construction of recombinant fowlpox virus coexpressing aiv ha and ndv f. for the construction of transfer vector pfgs11haf, aiv ha gene of f strain in puc18ha and ndv f gene of f48e8 strain in puc19f were removed and inserted into pfgs11. recombinant fowlpox viruses ( rfpv ) coexpressing aiv ha gene and ndv f gene were constructed by using different promoters of ps and pe / l. recombinant rfpvs were derived by dosper liposome - mediated transfection with the two transfer vectors on chicken embryo fibroblast ( cef ) monolayer cultures which were infected by wild type fpv chinese vaccine strain 282e4 3 - 4 hours earlier

    Puc18ha和sk質粒同時經hind 、 kpn酶切后連接得中間質粒skha ；將質粒skha用bamhi酶切回收ha基因插入到插入載體pfgs11中的bamhi位點，通過酶切鑒定獲得了pfgs11ha ；將含ndvf基因的質粒puc19f用hind 、 sal酶切經klenow酶補平插入到經sma酶切后的skifn中pe / l啟動子下獲得中間質粒skf ，再將質粒skf和puc18質粒先分別用ecor 、 xho酶切klenow補平，后再共同用sac酶切連接得puc18pelf ， sal酶切回收pe / l - f基因盒插入到pfgs11ha的sal位點，通過酶切鑒定獲得了pe / l - f與ps - ha同向的表達載體pfgs11haf 。
  19. The experimental results suggest that the cell disruption ratio can reach 97. 88 % using enzymolysis and nanometer - crushing machine, and the contents of amino acid in the extractives are also considerably raised

    試驗結果表明，酵母細胞先經酶解再結合納米破碎機破碎，可使酵母細胞壁的破碎率達到97 . 88 % ，大幅度提高了抽提物中氨基酸的含量。
  20. In this paper, we selected a widely used organophosphorous pesticide - - monocrotophos, used goldfish as model animal, and chose the vitellogenin induction of male fish as the main indicator, - gtp of sertoli cell in testes, the changes of microstructures of testis and ultrastructures of pituitary and testis as assistant indicators, we finally built up a screening system to effectively detect the environmental estrogen effect of organophosphorous pesticides, and reported that the monocrotophos had the activity of environmental estrogen for the first time in the world

    因此，本文選擇國內廣泛使用的有機磷農藥? ?久效磷，以金魚為模型生物，以雄性金魚卵黃原蛋白產生、精巢支持細胞- gtp酶活性，並輔以生殖軸線器官? ?垂體和精巢超微結構的變化，在國內首先建立了有機磷農藥環境雌激素效應的篩選體系，並首次確認久效磷具有環境雌激素活性。
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