酶切圖譜 的英文怎麼說
中文拼音 [qiētúpǔ]
酶切圖譜
英文
macrorestriction map-
It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent, genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study, genetic diversity in a. polytricha was higher than that in a. auricula : 4 in this study, a. fuscosuccinea had a higher homology to a. auricula than to a. polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a. auricular and a. fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a. auricula as a probe which hybridized with a. auricula and a. fuscosuccinea except a. polytricha, further confirming the veracity of the results from eric - pcr ; 7 in this study, isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity
本研究中，木耳屬2個種的2個菌株在its區域表現出較高的保守性， 4種限制型內切酶的酶切圖譜沒有顯示出多態性；增加內切酶種類及供試菌株數量，有可能獲得具有多態性的限制性內切酶酶切圖譜； 9本實驗中， its區域的真菌特異性引物與真核生物通用引物對于擴增效果無較大差異，擴增片段長度均為650bp左右； 10根據形態學實驗、 eric - pcr實驗以及southern雜交實驗的結果分析，紫木木耳屬種質資源的遺傳鑒定和遺傳多樣性評價耳極有可能是毛木耳種的一個變種; n .本研究中所用的gutc法是一種適用於木耳屬菌株基因組洲a快速提取的方法; 12 .傳統的形態學分類法和現代的分子生物學分類法，兩者的關系是相輔相成，互為驗證Proteins were identified with peptide mass fingerprinting using matrix - assisted laser desorption ionization time of flight mass spectrometry ( maldi - tof ms ) after tryptic in - gel digestion
差異蛋白經胰蛋白酶酶切后產生肽片段，再利用基質輔助激光解吸電離飛行時間質譜得到肽指紋圖譜來鑒定。It indicated that the chinese isolates belong to north american group. two pairs of different primers of orf7 were used to identify the genotype of prrsv isolates in china, and then compared with the reference isolates of north american and european serotype and modified live prrsv vaccine. the results further proved that prrsv prevalent in china belongs to b genetype. combining the restriction enzyme digestion patterns obtained from mini, hindi and sactt, we observed 2 distinct rflp patterns
在此基礎上，擴增各毒株的orf5基因，用mlu ， hinc和sac限制性內切酶切割orf5基因，通過這3種限制性內切酶獲得了各毒株的orf5基因限制性酶切圖譜，經rflp分析表明國內分離毒株與美洲型強毒株有著相同的rflp圖譜，而與疫苗毒的rflp圖譜存在明顯差異，進一步證明國內分離毒株的基因型屬於美洲型的強毒株。3. its shown from the results of dna digestion, hydantoinase gene amplification, rapd, eric - pcr etc, that the electrophoretic patterns of the products are obviously different form the original one
分別提取的基因組后進行酶切分析、海因酶基因的擴增、 rapd 、 eric - pcr等檢測，兩者均呈現不同的電泳圖譜。The study indicates that mitochondrial dna sequence analysis is a useful tool to associate the larval and adult stages of trichoptera, in turn, it will be valuable for health assessment of stream ecosystems
根據原雙棲紋石蛾成幼蟲的pcr反應電泳圖譜、序列同源性和酶切圖譜的一致性，基本確認了其成幼蟲聯系。Nucleotide acid sequence analysis indicates that two construts were correct
對其進行了限制性酶切圖譜分析。Based on the result of numerical taxonomy, 16s rdna pcr - rflp were applied to 12 isolates and 9 reference strains
一些菌株如ccbau61116 、 ccbau41069 、 ccbau23168等具有專一的酶切圖譜類型。Recovered the agarose and identified by agarose gelelectrophoresis. the producys of pcr fragments and pcambial303 plasmid ligation were transformed into e. coli ( dh5a ). the result of pcrof positive recombinant and restriction analysis demonstrated that the plant expressing vector of tps is attained
Pcr產物經回收后，經瓊脂糖凝膠電泳鑒定后，與pcambia1303連接並轉化大腸桿菌dh5a ，陽性重組子經pcr和限制性內切酶酶切圖譜分析，表明已獲得海藻糖- 6 -磷酸合成酶基因的植物表達載體。Then the genetic distance and shannon information index among these 13 strains were obtained by using the software popgen2 on the basis of pcr - rflp patterns. the phylogenetic tree was constructed by using the upgma algorithm method ( mega2 software package ). the shannon information index indicated that there was a higher genetic diversity among the frankia strains and some strains had a big genetic distance with others
根據酶切圖譜，通過popgen2軟體計算各供試菌株之間的遺傳距離；用shannon信息指數計算種群遺傳多樣性；利用upgma法（ mega2軟體）對所獲得的遺傳距離矩陣進行聚類分析，得到13株供試frankia菌株間系統發育的聚類分析樹狀圖譜。Using the monoclonal antibody to rev and the anti - sera against the rev env gp90 - gst fusion protein. the molecular cloned virus was detected by ifa. we also amplified the gp90 from the cells infected with the molecular cloned virus by polymerase chain reaction. all these results indicated the recombinant plasmid containing the total rev genome cdna is infectious
對snv株前病毒全基因組cdna克隆進行酶切，將酶切產物分別克隆進puc18中，分別將各個亞克隆進行測序，按照酶切位點和已知的部分序列以及rev物理圖譜將測得的序列進行拼接，完成了rev全基因組序列。The restriction map of phzl318 carrying the entire add gene cluster from s. avermitilis nrrl8165 was made. its two subclones phz2104 and phz2105 were introduced into s. lividans mutant zx1
製作了攜帶完整add基因簇的phz1318的限制內切酶圖譜，根據酶譜進行亞克隆得到phz2104和phz2105 。One hundred and seven isolates belonging to 12 chinese armillaria species and their relatives occurring in japan, europe and north america were subjected to a restriction analysis of intergenic spacer region ( igs - rflp ) using four restriction enzymes. the analysis revealed a total of 36 different rflp patterns
用pcr擴增12個中國蜜環菌生物種及其相關的歐洲、北美和日本蜜環菌種的107個菌株的基因間隔區（ igs ）序列，用alui 、 hae 、 hinfi 、 tagi4種內切酶進行消化，結果產生了36個不同的rflp圖譜類型。The restriction map of the plasmid pbl29 was tentatively constructed according as the molecular weights of fragments of plasmid pbl29 digested with different enzymes. analysing the sequence of the plasmid pbl29 tested, we found that the mol ecular weight of the plasmid pbl29 is 371 lbp, that it s many unique restriction endonucleases, km resistance genes and abundant a / t base pairs of replicating site are on the plasmid pbl29
同時根據限制性酶切各片段的分子量作出了質粒pbl29的內切酶圖譜。並對質粒pbl29進行測序和分析，證明了質粒pbl29大小為3711bp ，具有大量的單酶切位點、編碼卡那黴素抗性基因以及富含a t序列的復制起點。But the results of restriction enzyme reaction are not satisfied. there may be two reasons which lead to these results : one is that the restriction enzymes are not suitable for this plasmid, that is to say there are no appropriate cut sites on the plasmid for these restriction enzymes ; and the other is that the purification of the plasmid is not enough for restriction enzyme reaction, and some impurity affects the last results
為了建立質粒的物理圖譜，我們對所提取的質粒進行了限制性酶切消化，摘要但是未能取得滿意的結果，原因可能有兩個：一，所選擇的酶不合適，在質粒上沒有合適的酶切位點；二，質粒的純度不夠，影響了酶切反應的結果。By comparing restriction maps of plasmids possessed in these clones, 5 clones ( clones 1, 4, 5, 6, and 8 ) were found to contain the same chitinase gene, while the other three clones ( clones 2, 3 and 9 ) contain different chitinase genes one another
經底物反應和限制性內切酶圖譜分析，確定其中的clone - 1 ， 4 ， 5 ， 6 ， 8含有相同的幾丁質酶基因；而clone - 2 ， 3 ， 5 ， 9四株重組菌則含有不同的幾丁質酶基因。分享友人