酶受體測定 的英文怎麼說
中文拼音 [shòutǐcèdìng]
酶受體測定
英文
enzyme receptor assay-
The peroxidase activity of variant t220x was obviously higher than acceptor lu22 at seedling stage. the result of peroxidase electrophoresis indicated that there were difference both in the depth and in the amount of zymogram between lu22 and t220x. so it was concluded that the variety of peroxidase was the result of change of heriditary substance
小麥幼苗期過氧化物酶活性的測定顯示，變異體t220x的酶活性高於受體魯22 ；過氧化物酶電泳顯示， t220x和魯22過氧化物酶在酶帶深淺和數目方面都存在差異，說明外源遺傳物質已影響到過氧化物酶的變化。This paper stuffed with twelve important grain and vegetable crops, studied the injury symptom, dose reaction, injury threshold value and influential factor of main pollutant so2 on various plants, tested the dynamic transformation of pod, cat, mda, soluble protein, free pro and chlorophyll of resistant plant and sensitive of these physiological biochemical transformation with plant resistant ability. meanwhile, simply studied the protective role of the five compounds on plant. the result indicated the followings
本實驗以12種重要的糧食和蔬菜作物為研究對象，研究了主要大氣污染物二氧化硫（ so _ 2 ）對不同植物的傷害癥狀、劑量反應、傷害閾值以及影響因素，測定了抗性和敏感植物在受到so _ 2污染后植物體內過氧化物酶（ pod ） 、過氧化氫酶（ cat ） 、丙二醛（ mda ） 、可溶性蛋白質、游離脯氨酸和葉綠素的動態變化，並分析了這些生理生化變化和植物抗性的相互關系，同時還對5種化合物溶液對植物的保護作用進行了初步研究，結果表明： 1In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein
經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。Gene expression of neural related genes was identified by semi - quanti - tive rt - pcr analysis and genechip assay. 4 and 10 days after neural induction, gene expression pattern was analysed by genechips, which showed the expression of some neural stem cells and mature neurons specific and related genes, repectively. especially the expression of gabar and glutamate dehydrogenase ( gad ), which meant the induced cells could be gabanergic neurons
2 .基因晶元檢測到未分化escs 、神經幹細胞及成熟神經細胞的相關基因，並由半定量rt一pcr證實基因晶元檢測未分化細胞表達胚胎幹細胞特異基因;誘導后第4天細胞高表達神經幹細胞特異性基因;誘導后第10天細胞高表達成熟神經細胞特異性基因，且有gaba受體、谷氨酸脫梭酶（ gad ）表達，說明誘導后細胞大多為以ba能神經元。Naphthol green b, which is low cost and easy obtained, has been shown to be an efficient mediator, promoting electron transfer from glucose oxidase to graphite electrode. the naphthol green b has low formal potential, which can reduce the overvoltage of the h2o2 oxidation to eliminate electrochemical interference. rapid response, oxygen independence and high sensitivity are shown by the naphthol green b mediated biosensor
將萘酚綠b作為電子媒介體與葡萄糖氧化酶一起固定於電極，研製出性能優良的新型葡萄糖生物傳感器，其電子傳遞快速，響應時間短，不受氧氣濃度的影響，檢測電位低，抗干擾能力強，靈敏度高。Methods : in cultured lung explants without serum, the lipid component synthesis of pulmonary surfactant was evaluated in [ 3h ] - choline incorporation ; mrna content of phosphocholine cytidylyltransferase ( cct ) in lung explants was investigated in rt - pcr ; the changes of the ultrastructure of the at ii cells were observed with electron microscope ; the expression of nmdar1 subtype was observed in immunohistochemistry staining ; nitric oxide synthase ( nos ) activity, nitric oxide ( no ) content, superoxide dismutase ( sod ) level, malondialdehyde ( mda ) content and lactae dehydroase ( ldh ) level were determined by biochemistry methods. results : 1. influence of glutamate on synthesis of the lipid component of pulmonary surfactant ? with l - arginine, glu inhibited [ 3h ] - choline incorporation with good dose - dependence and time - dependence ; ( 2 ) mrna content of cct of the glu treatment groups was decreased ; ( 3 ) glu increases the release of ldh in cultured lung explants ; ( dwith electron microscope histochemistry, glu induced the changes of the ultrastruture of at ii iv cells
方法：採用成年大鼠肺組織無血清培養，運用[ ~ 3h ] -膽堿摻入法測定ps主要脂質磷脂酰膽堿（ pc ）合成量； rt - pcr擴增檢測肺組織中pc合成限速酶磷酸膽堿二胞苷酰基轉移酶（ cct ） mrna含量；透射電子顯微鏡法觀察肺泡型上皮細胞和ps系統超微結構的變化；免疫組織化學染色檢測glu的受體nmdar1亞單位的表達；生化測定肺組織乳酸脫氫酶（ ldh ）釋放量和肺組織勻漿中一氧化氮合酶（ nos ）活性、一氧化氮（ no ）生成量、超氧化物歧化酶（ sod ）水平以及丙二醛（ mda ）含量。The purified products were cloned into pgem - t - easy vector successfully, the cloned plasmids were transformed into e. coli. tgl. the specific recombinant plasmid was identified by molecular weight, pcr and restriction endonuclease analysis. the results indicated that the resultant construct contained the gene of interest hn at right orientation of the insert
經瓊脂糖電泳檢測，將大小與預計分子量一致的片段純化后連接到pgem - t - easy克隆載體中，再轉化大腸桿菌tgi感受態細胞，得到的轉化子經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。The purified production was cloned into pmd18 - t vector. the cloned plasmids were transformed into jm109. the specific recombinant plasimid was identified by molecular weight, pcr and restriction endonuclease analysis. the results indicated that the resultant construct contained the gene of ibdv a fragment at right orientation
經瓊脂糖電泳檢測，將大小與預計分子量一致的片段純化后連接到pmd18 - t載體中，再轉化到jm109感受態細胞，得到的轉化子經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆，結果表明，得到的陽性重組子中含有a節段全長基因，插入到載體中的方向正確。分享友人