酶標抗體 的英文怎麼說

中文拼音 [biāokàngtǐ]
酶標抗體 英文
enzyme labelled antibody
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 標 : Ⅰ名詞1 [書面語] （樹梢） treetop; the tip of a tree2 （枝節或表面） symptom; outside appearance; ...
  • 抗 : Ⅰ動詞1 （抵抗; 抵擋） resist; combat; fight 2 （拒絕; 抗拒） refuse; defy 3 （對等） contend with...
  • 體 : 體構詞成分。
  1. A rabbit was infected with a cloned yntatl, blood was collecting from from the rabbit every 3 days after infection within 30 days, 10 clonal trypanosome populations were gotten, infecting a new rabbit by the last non - cloned trypanosome population. repeated above all, thus infected 5 rabbits sequentially. twenty different vats ( variant antigen type ) were monitored and characterized from those fifty mono - clonal populations by indirect immunofluorescence test ( ift ) and avidin biotin enzyme immunoassay ( abc - eia )

    用伊氏錐蟲雲南水牛單克隆株yntat1感染兔，感染后30天內，每3天從兔血中分離錐蟲並單蟲克隆，最後一個未單蟲克隆的蟲株感染另一隻兔，重復以上操作，這樣順序感染5隻兔子，共獲得50個單克隆錐蟲種群（ tp ） ，經間接免疫熒光和abc酶標試驗鑒定共為20個抗原性互不相同的抗原變異體（ vats ） 。
  2. The complex formed by cnbr - activated alginate and antibody is aggregated to the surface of the paraffin - graphite - chitosan electrode by electrostatic adsorption ( coacervation ). the concentration of sjag can be detected by determining the redox current of o - aminophenol, which oxidized by h2o2 in the presence of hrp. moreover, the immunosensor shows some improved performances including high sensitivity, selectivity and less non - specific adsorption

    褐藻酸鈉?抗體復合物通過靜電吸附作用被凝集到含石墨?石蠟?殼聚糖組分的電極表面，然後與抗原和酶標抗原進行競爭反應，以鄰氨基酚為電子媒介，通過測定酶催化下雙氧水對其氧化的電流大小來間接測定抗原的濃度。
  3. If the report is medium a few enzymatic index are higher, but the examination of hbvdna is normal, it is electropositive that the examination of two half - and - half has antibody only, so this kind infectious

    假如報告中幾項酶的指標都比較高，但是hbvdna的檢查是正常的，兩對半的檢查只有抗體是陽性，那麼這種有傳染性嗎？
  4. Because of the cleavage site of enterokinase and cnbr was designed in the middle of thioredoxin and cmiv, the expressed peptides of the mutation of cmiv could be cuted down from the fusion protein by enterokinase or cnbr

    由於硫氧還蛋白和抗菌肽之間設計了腸激酶（ enterokinase ）切割位點和cnbr切割位點，通過對該表達的融合蛋白的切割，可得到目標抗菌肽cmiv突變體多肽分子。
  5. The quality of an enzyme immunoassays depends very much on the purity of the antigen or hapten used for conjugation, the specificity of the antibody and the choice of a suitable enzyme label

    酶免疫分析技術的質量依賴于抗原的純度、抗體的特異性、合適標記酶的選用，其靈敏度取決于標記酶的高度純化和高轉化率。
  6. Test 3 : detected activity of serum and immunoglobulin samples by indirect elisa test, rabbit antibody against foxes ' igg and hen ' s labeled with hrp igy was used in indirect elisa test

    試驗三：間接elisa檢測的抗體活性。用過氧化物酶標記的兔抗狐igg和雞igy抗體進行間接elisa測定獲得的樣品的活性。
  7. The development of renewable amperometric is a possible way to circumvent the problem. here, antigen ( antibody ) is immobilized with graphite ( carbon ) and carrier on a transducer, the analyze is measured through on enzyme catalytic reaction after sandwich or competitive immunoreaction

    將抗原（抗體）與石墨或者碳固定在載體材料中，在一個競爭性的或者夾心式的免疫反應后，將酶標抗原（抗體）鍵合在傳感器表面，通過一個酶催化反應來確定待測抗原（抗體）的濃度。
  8. The development of renewable amperometric is a possible way to circumvent the problem. here, antigen ( antibody ) is immobilized with graphite ( carbon ) and carrier on a transducer, the analyze is measured through on enzyme catalytic reaction after sandwitch or competitive immunoreaction. the surface of immuno - sensor can be renewed by used in a new immunoassay

    將抗原（抗體）與石墨或者碳固定在載體材料中，在一個競爭性的或者夾心式的免疫反應后，將酶標抗原（抗體）鍵合在傳感器表面，通過一個酶催化反應來確定待測抗原（抗體）的濃度。
  9. Polymyositis ( pm ) and dermatoyositis ( dm ), devastating inflammatory muscle diseases, are associated with the myositis - specific anti - jo - 1 autoantibody, found in 25 - 40 % of such patients. anti - jo - 1 antibodies react with histidyl - trna synthetase

    抗組氨酰? trna合成酶抗體，臨床上又稱抗jo - 1抗體，是診斷這類疾病的標志性抗體。
  10. The wells of elisa plate were coated with pab ( 100ng / l ) against h3n2, then phage was added to the wells. after incubation, the wells were washed vigorously with tbst to remove nonbinding phage. phage bound to the antibody were eluted with 0. 2mol / l glycine - hcl ( ph2. 2 ) for 10 min at room temperature and neutrialized with 2mol / l tris - hcl ( ph9. 1 )

    以抗h3n2流感病毒的多克隆抗體（ 100ng l ）包被酶標板，加入制備好的肽庫，用tbst洗去非特異結合的噬菌體，加0 . 2mol l甘氨酸-鹽酸（ ph2 . 2 ） ，室溫放置10min以洗脫特異結合的噬菌體， 2mol ltris - hcl （ ph9 . 1 ）中和后，取2 l噬菌體接種大腸桿菌xl1 - blue菌進行空斑滴定，其餘噬菌體擴增後用于下一輪篩選，共重復3輪淘洗。
  11. The determination of human thymidine kinase ( htk ) in human serum, which is a key indicator of cancers can give information for the diagnosis and treatment of the malign diseases. the protein a layer was first self - assembled onto the gold electrode surfaces of quartz crystals, the monoclonal antibodies were then orientedly immobilized through the specific binding between the fc terminals of the antibodies and the self - assembled protein a. with this sensor, the affinity constant of antigen - antibody binding was estimated to be 1. 85 106 l / mol according to the scatchard ’ s plotting method, which proved the high bioactivity of antibody. finally, an amplified piezoelectric immunosensor was designed to determine the htk in

    實驗中將蛋白a吸附於鍍金壓電石英晶體電極表面，用於定向固定htk單克隆抗體，成功研製了檢測htk的壓電石英晶體傳感器，並基於標準scatchard繪圖法，計算出免疫反應的親和常數為1 . 85 106l / mol ，證明該單克隆抗體具有較高的免疫活性;同時基於酶催化沉澱技術，設計了的檢測htk的質量放大壓電石英晶體傳感器，該傳感器可在0 . 1 - 10ng范圍內對htk進行定量檢測，應用此傳感器成功地對5種癌癥病人血清中htk的濃度進行了測定，實驗結果為癌癥的臨床診斷與治療提供了參考。
  12. In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company

    實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 （ 1 : 1 ）混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑（ upofectalnineplusreageni ）和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 （ do一7 ）蛋白（即用型） ;鼠單克隆抗體p21waf ， （即用型） ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰（濃縮型） ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。
  13. It was proved that the amount of immobilized antibody and the immunoactivity of bound antibodies could be well improved by colloidal au. hrp labeled antibody reacted with antigen, then hrp biocatalyzed dab ( 3, 3 ’ - diaminobenzidine ) in the presence of h _ 2o _ 2, resulting in an insoluble product onto the electrode surface, to achieve an obviously decreased frequency

    在h _ 2o _ 2存在下，通過標記在抗人igg抗體上的辣根過氧化物酶（ hrp ）催化底物3 ， 3 』 -聯苯二胺（ dab ） ，反應生成不溶性產物沉積到石英晶振的au電極表面，達到質量放大的目的。
  14. Amplified in e. coli xi - blue, the eluted phage in the third rounds was poured onto lb / iptg / xgal plate. we selected randomly 18 clones and amplified them, then confirmed positive clones by elisa

    經雙抗體（流感病毒的多抗和辣根過氧化物酶標記m13噬菌體抗體）夾心elisa鑒定的陽性克隆有12個，分別將其純化、並進行dna序列分析。
  15. Using o - aminophenol as a substrate, hrp as label, a competitive immunoassay is used to determine the concentration of the analyte. we also reported the cyclic voltammetric behavior of 1 - o - allyl - 4 - hdroxy - anthraquinone ( oahaq ) and the preparation and electrochemical characteristics of electrode modified by 2 - pyridinamine

    以辣根過氧化酶作為標記物，鄰-氨基酚作為酶催化反應的底物，採用一個競爭性免疫分析來確定待測抗原（或抗體）的濃度。
  16. A dot - enzyme - linked immnosorbent assay ( dot - elisa ) for the detection of antidodies against newcastle diesease virus ( ndv ) was established by using purified ndv and self - made enzyme - labeled anti - chicken igg, the mehod was also evaluated through it ' s application

    本研究用提純的新城疫病毒和自製的酶標抗體建立了檢測雞新城疫抗體的dot - elisa方法。試驗中用f _ （ 48 ） e _ 9株和lasota株提純抗原並對兩者進行了比較。
  17. Anti - igg antibody was immobilized onto the surface of gold electrode modified with a thin layer of protein a. biotin labeled anti - igg antibody and hrp - streptavidin were premixed to form network complex to be used as probes for amplifying the sensing response of antibody - antigen interaction

    抗體通過單層蛋白a固定到金電極表面，預先處理好的生物素化抗體和辣根過氧化物酶（ hrp ）標記的親和素形成的網狀復合物作為免疫反應信號的放大探針。
  18. The series of enzyme - labelled reagent kits the enzyme - labelled reagents adopt the enzyme - labelled monoclonal antibody technology, and have high sensitivity, excel distinctiveness and accurate results. a portion of kits can determine the amounts of antigen or antibody. the series widely apply to clinical diagnosis in hospital and general survey

    酶標檢測試劑系列是採用單克隆抗體酶標記技術研製而成，具有靈敏度高特異性強結果準確等優點，部分產品還可定量檢測，廣泛適用於醫院臨床檢測和人群普查。
  19. Methods : sandwich elisa assay was used, w6 / 32 mcab serving as solid phase antibody and 3 2m antibody as the first antibody. the second antibody - hrp conjugate was added for coloration. standard curve was obtained by shla - i standard reagent in serial dilution. the amount of shla - i in the samples was determined : 1

    方法：以w6 32包被酶標板，捕捉樣品中可溶性hla ，加入一抗2m抗體，再加酶標二抗及底物顯色。根據可溶性hla -的不同濃度標準品顯色后的od值繪制標準曲線： 1
  20. The optimum conditions of dot - elisa was determined : the optimal coating concentration was 8. 9 u g / ml, 1 : 400 was the best working concentration of hrp - laleled rabbit antichicken igg, the other steps and reagentes were also optimized

    抗原最適包被濃度為8 . 9 g / ml ，酶標抗體最適工作濃度為1 ： 400 ，確定了其他反應試劑和各步的最佳反應時間。
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