酶解圖譜 的英文怎麼說
中文拼音 [jiětúpǔ]
酶解圖譜
英文
finger print-
The effort of manganese removal was studied and the kinetics of manganese removal was tried to establish. the factors of dissolved oxygen concentration, fe2 + concentration, ph, p concentration and closing of the filter were studied to evaluate their effort for biological manganese removal, and the correlation of residual manganese and oxidation - reduction potential was also discussed. as the iron content of water was high, experiment results showed that the reaction was zero order, as the iron content of water was low, the reaction was first order. the time needed for the cultivation of biological manganese removal was 60 70 days. the filter operated at the filtration rate of 8 10m / h, silica sand of effective size 0. 95 1. 25mm filled the filter to a depth of 1200mm
試驗結果表明，成熟后濾砂表面濾膜的x射線衍射圖譜與mno _ x ? 5h _ 2o （ x = 1 . 86 ）的x射線衍射圖譜一樣，濾膜成熟后的結構在進水物質不發生變化的情況下不發生變化;合適的碳磷比對生物除錳有明顯的促進作用，試驗條件下的投磷量不會對出水造成二次污染;生物除錳需要亞鐵的參與，亞鐵的存在除了能夠促進微生物分泌胞外酶並刺激其活性外，還通過鐵離子的變價傳遞電子，催化錳離子的氧化反應，從而促進對二價錳的降解。For protein identification, proteins spots of interest on the gels stained with colloidal coomassie brilliant blue g - 250 were excised, digested in - gel with trypsin, and analyzed by peptide mass fingerprinting ( pmf ) with matrix assisted laser desorption / ionization - mass spectrometry ( maldi - ms ). erp60, trypsinogen, proelastase, lipaseandso on were identified. this will enable us to present an overview of the proteins expressed in rat pancreas tissues and lays the basis for subsequent comparative proteome analysis studies with pancreas development
從中隨機選擇一些差異蛋白質點，進行基質輔助激光解吸-電離飛行時間質譜（ maldi - tof - ms ）測定其膠內酶解后的肽質指紋圖譜，用mascot軟體查詢swiss - port數據庫，初步鑒定為一些與生長發育、物質代謝、細胞因子、信號轉導等有關的蛋白質，如erp60 、 trypsinogen 、 proelastase 、 lipase等。Proteins were identified with peptide mass fingerprinting using matrix - assisted laser desorption ionization time of flight mass spectrometry ( maldi - tof ms ) after tryptic in - gel digestion
差異蛋白經胰蛋白酶酶切后產生肽片段，再利用基質輔助激光解吸電離飛行時間質譜得到肽指紋圖譜來鑒定。These were shown to emulate ion chromatograms produced in a subsequent run without the digestion element, based on protein elution
可以仿效一個序列運行的離子色譜圖，而不需要基於蛋白洗提的酶解成分。57 protein spots out of about 1000 detectable spots on the 2 - d gels were indentified by the following two methods : l ) n ~ terminal edman degradation microsequencing after the protein spots were electro - transferred to pvdf membrane. 2 ) maldi - tof - ms peptide fingerprint analysis of the protein spots and protein database searching. the 2d protein maps of the rice spikelet during the sterile and fertile stages were compared
二是通過原位酶解，抽提蛋白質片段進行maldi - tof質譜分析，利用肽質量指紋圖數據在數據庫中進行檢索，在雙向電泳凝膠上取了57個點進行分析，有34個蛋白質點在數據庫得到歸屬鑒定。In he - 3 and de - 3, 16 protein spots that were absolutely different ( only expressed in diploid embryos but not in haploid embryos or vice versa ) and 16 protein spots that were up - and down - r gulated were identified unambiguously. in he - 2 and de - 2, 20 protein spots that were absolutely different ( only expressed in diploid embryos but not in haploid embryos or vice versa ) and 5 protein spots that were up - and down - regulated were identified unambiguously, some of these different proteins are correlative with eyes development, nerve development, development regulation, cell differentiation and maintenance, arthromere formation and signal transduction. it shown that gene expression have significant difference between diploid and haploid during embryos developing
分別選取其中70個分辨好的差異蛋白質點進行原位胰蛋白酶酶解，酶解的肽片段用基質輔助激光解析電離分行時間質譜（ maldi - tofms ）得到肽質指紋圖譜，再在網上peptident軟體中搜索swiss - prot和trembl數據庫，初步鑒定了he - 3和de - 3上32個差異蛋白質點，其中包括16個表達量上有差異的蛋白質點（ p 0 . 05 ）和16個有無表達的差異蛋白質點，並且初步鑒定了he - 2和de - 2上25個差異蛋白質點，其中包括5個表達量上有差異的蛋白質點（ p 0 . 05 ）和20個有無表達的差異蛋白質點。分享友人