酶譜法 的英文怎麼說
中文拼音 [pǔfǎ]
酶譜法
英文
zymography-
It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent, genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study, genetic diversity in a. polytricha was higher than that in a. auricula : 4 in this study, a. fuscosuccinea had a higher homology to a. auricula than to a. polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a. auricular and a. fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a. auricula as a probe which hybridized with a. auricula and a. fuscosuccinea except a. polytricha, further confirming the veracity of the results from eric - pcr ; 7 in this study, isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity
本研究中，木耳屬2個種的2個菌株在its區域表現出較高的保守性， 4種限制型內切酶的酶切圖譜沒有顯示出多態性；增加內切酶種類及供試菌株數量，有可能獲得具有多態性的限制性內切酶酶切圖譜； 9本實驗中， its區域的真菌特異性引物與真核生物通用引物對于擴增效果無較大差異，擴增片段長度均為650bp左右； 10根據形態學實驗、 eric - pcr實驗以及southern雜交實驗的結果分析，紫木木耳屬種質資源的遺傳鑒定和遺傳多樣性評價耳極有可能是毛木耳種的一個變種; n .本研究中所用的gutc法是一種適用於木耳屬菌株基因組洲a快速提取的方法; 12 .傳統的形態學分類法和現代的分子生物學分類法，兩者的關系是相輔相成，互為驗證Hplc determination of inulin zymohydrolysis products
高效液相色譜法測定菊粉酶解產物Fruit and vegetable juices. enzymatic determination of l - matic acid l - malate content. nadh spectrometric method
水果和蔬菜汁. l -蘋果酸含量的酶催化的測定. nadh光譜法Method for enzymatic determination of l - malic acid l - malate content of fruit and vegetable juices - nadh spectrometric method
果汁和蔬菜汁中l -蘋果酸的酶催化測定法. nadh光譜法Fruit and vegetable juices. enzymatic determination of d - malic acid content. nad spectrometric method
水果和蔬菜汁. d -蘋果酸含量酶催的測定. nad光譜法It was found that b - 6 isolated by the method of distinct zone of clearing in cellulose - congo red agar medium combined with measuring the enzyme activity of liquid culture filtrates had comparatively higher cellulase activity. by measuring activity of cellulase of strains growing in medium with different carbon sources and of washed mycelium induced by different carbon sources in certain time, it found that the formation of cellulase was regulated by the nature of the carbon source used for b - 6 and as3. 3711
真菌纖維素酶是一種誘導酶，碳源同時也是主要的誘導物來源，為了研究碳源對真菌纖維素酶合成的誘導機理，本文利用液體生長培養和洗滌菌絲誘導培養法研究了不同碳源對兩菌株的誘導特性，並用電泳分析法研究了不同碳源的誘導酶譜。Determination of disulfide bonds in protein by enzymatic digestion and mass spectrometry
酶切質譜法測定蛋白質的二硫鍵Huwentoxin xi ( hwtx ~ xi ), a serine protease ! inhibitor, consists of 55 amino acid residues with three disulfide bridges. the toxin was isolated from the venom of the chinese spider ornithochoctonus huwena by ion - exchange chromatogram - phy and reverse phase high performance liquid chromatography
本文報道從虎紋捕鳥蛛（ ornithochoctonushuwena ）粗毒中，應用陽離子交換和反相高效液相色譜的方法分離到一種胰蛋白酶抑制劑，命名為huwentoxin - （ hwtx - ） 。According to the comparation among the three kinds of crude extraction methods including acid dissolve, enzymolysis and neutral salt solution. the method of enzymolysis combined with hplc was chosen to prepare cp i. the physico - chemical property of, cp i was identified
以豬皮為材料，比較酸溶法、酶解法、中性鹽溶液法三種粗提方法，選擇得率和純度都較高的酶解法為最佳粗提法，結合高效液相色譜進行cp的分離純化。Fos contain mixture of gf2, gf3 and gf4 sugars ( where g = glucose molecule and f = fructose molecule ) and a dp ( “ degree of polymerization ” ) of 3 - 5 ( “ neosugar ” ), are not naturally - occurring but are enzymatically synthetized from sucrose by action of an enzyme from the fungus aspergillus niger
詳細說明：是以蔗糖做底物，採用呋喃果糖苷酶轉果糖基作用，在蔗糖分子上以（ 1 2 ）糖苷鍵上與1 - 3個果糖分子結合，形成的蔗果三糖（ gf2 ） 、蔗果四糖（ gf3 ） 、蔗果五糖（ gf4 ）屬于果糖和蔗糖構成的直鏈雜低聚糖，在形成的產物中還有果糖、葡萄糖和未反應完全的底物蔗糖，採用色譜法除去單糖和雙糖制得高純度的低聚果糖。The results showed mn and ni complexes possibly bind to dna by the mode of interaction, whereas zn complex possibly bind to dna by the modes of interaction and electrostatic binding. 5. in addition, we conjugated cleavage system with recognize system and analyzed joint products by hplc, which provide experimental basic for design of dual effects cleavage
此外，本文還選用咖啡酸純品來突破切割體系與識別體系（用氨基臂修飾的寡聚脫氧核苷酸）的連接，並用高效液相色譜法分析其偶聯產物，為今後設計併合成一種具有特異識別和高效切割雙重功能的人工核酸酶提供了實驗基礎。Abstract : the determination of aminoglycoside antibiotics in the biological fluid has been reviewed. the methods mainly included bioassay, thin - layer chromatography, high - performance liquid chromatography ( hplc ) and a variety of immunoassay ( ia ), for example, enzyme immunoassay, fluorescence polarization immunoassay, quenching fluorescence immunoassay, chemiluminescent immunoassay ， etc., in which hplc and ia were used more often
文摘:綜述了氨基糖苷類抗生素的體內分析方法.主要有：生物學分析法；薄層色譜法；高效液相色譜法；差示脈沖極譜及各種免疫法，如酶免疫法，熒光偏振免疫法，膠乳凝集抑制速率比濁法等.其中高效液相色譜法和免疫法應用較多Fruit and vegetable juices. enzymatic determination of citric acid citrate content. nadh spectrometric method
水果和蔬菜汁.檸檬酸含量的酶催化的測定. nadh光譜法Fruit and vegetable juices. enzymatic determination of d - isocitric acid content. nadph spectrometric method
水果和蔬菜汁. d -異檸檬酸含量的酶催化的測定. nadph -光譜法Fruit and vegetable juices. enzymatic determination of d - glucose and d - fructose content. nadph spectrometric method
水果和蔬菜汁. d -葡萄糖和d -果糖含量的酶催化的測定. nadph -光譜法Phopholipase c - 1 ( plc - 1 ) is widely known to play an important role in regulating cell proliferation and differentiation, development of the organisms, cell transformation and oxidative stress. till now, the mechanism how phopholipase c - 1 acts can not be thoroughly illustrated, nor has the interaction between plc - 1 pathway and other signal pathways been systematically reported. this research chose 2 - de + ms as the basic method from all kinds of proteomics strategies and compared the total protein expression map of mef genetically deficient in plc - 1 ( plc - 1 - / - ) to that of wild type mef ( plc - l + / + ) aimed to find some protein spots differentially expressed, thus we can discuss the impact of knockout of plc - 1 on signal transduction initiated by growth factors such as egf comprehensively. in this way, we can study the biological function of plc - 1 and mechanism of plc - 1 pathway indirectly, which will contribute a lot to further analysis
鑒于plc - 1發揮上述作用的機制尚未完全闡明， plc - 1通路與其他信號通路間的交聯和代償尚無系統報道，又因為以往的研究方法不夠全面，本研究以野生型小鼠胚胎成纖維細胞（ plc - 1 ~ （ + / + ） ）和缺失磷脂酶c - 1的小鼠胚胎成纖維細胞（ plc - 1 ~ （ - / - ） ）為研究模型，在眾多蛋白組學策略中選擇了雙向電泳+質譜（ 2 - de + ms ）作為研究手段，通過對比表皮生長因子（ egf ）刺激24小時後上述兩種細胞的總蛋白質表達差異，全面地探討敲除plc - 1對生長因子誘導的信號傳遞的影響，從而間接研究plc - 1生物學作用、信號傳遞機制及其代償情況，為后續的深入研究打下基礎。Then the genetic distance and shannon information index among these 13 strains were obtained by using the software popgen2 on the basis of pcr - rflp patterns. the phylogenetic tree was constructed by using the upgma algorithm method ( mega2 software package ). the shannon information index indicated that there was a higher genetic diversity among the frankia strains and some strains had a big genetic distance with others
根據酶切圖譜，通過popgen2軟體計算各供試菌株之間的遺傳距離；用shannon信息指數計算種群遺傳多樣性；利用upgma法（ mega2軟體）對所獲得的遺傳距離矩陣進行聚類分析，得到13株供試frankia菌株間系統發育的聚類分析樹狀圖譜。The distribution and accumulation of three kinds of heavy metals including lead, copper and mercury in tissues and cells of several organs, and their effects on activity of digestive enzymes and alkaline phosphatase of macrobrachium nipponense were studied with light microscopy, transmission electron microscopy, cytochemical method, atom absorption spectrum, mass spectrum analysis and enzyme analytical method
本文應用組織學、組織化學、透射電鏡、原子吸收光譜分析和酶學分析等方法，研究了鉛、銅、汞等三種重金屬在日本沼蝦各主要器官細胞內的分佈和積累以及對日本沼蝦消化酶和堿性磷酸酶活性的影響。Fruit and vegetable juices - enzymatic determination of d - and l - lactic acid lactate content - nad spectrometric method
水果汁和蔬菜汁. d和l乳酸酯中酶的測定. nadg光譜儀法En 12631 fruit and vegetable juices. enzymatic determination of d - and l - lactic acid lactate content. nad spectrometric method
水果和蔬菜汁. d和l -乳酸含量酶法測定. nad度譜儀法分享友人