酸性數碼 的英文怎麼說
中文拼音 [suānxìngshǔmǎ]
酸性數碼
英文
acid techno- 酸 : 酸構詞成分。
- 性 : Ⅰ名詞1 (性格) nature; character; disposition 2 (性能; 性質) property; quality 3 (性別) sex ...
- 數 : 數副詞(屢次) frequently; repeatedly
- 碼 : Ⅰ名詞(表示數目的符號或用具) a sign or object indicating number; code Ⅱ量詞1 (指一件事或一類的...
- 酸性 : [化學] acidity; acidness; acerbic; acidic property酸性材料 acid material; 酸性促進劑 acid acceler...
- 數碼 : numerical code; digit; figure
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The cloning cdna fragment was extracted from positive clones and sequenced. the results showed that the cdna fragment was 816bp in size, encoding a protein which included 272 amino acids. the sequence homology analysis was carried out via the software blast 2. 0 network service in the four large databases - genbank, embl, ddbj, pdb, which had recorded 1 337 978 nucleotide and protein sequences. the results of the analysis indicated that the nucleotide homologous rates between the rubber tree etr and 15 recorded etrl of other plants ( mango, passion fruit, persia plum, strawberry, grape. . etc ) were 75 % - 80 % ; the protein homologous rates between the rubber tree etrl and these recorded etrl genes were 90 % - 95 %. from the results mentioned above, we could confirm that the cdna of rubber tree etrl had been cloned
從陽性克隆子中提取克隆片段,經序列測定分析,結果表明,克隆片段的cdna大小為816bp ,編碼的蛋白質包含272個氨基酸。基因序列通過blast2 . 0networkservice軟體對genbank , embl , ddbj , pdb四個大型數據庫中記錄的1337978條核酸和蛋白質序列進行序列相似性檢索,結果表明與芒果、一西番蓮、波斯梅、草毒、葡萄、西洋梨等15種已報道的植物的etrl基因cdnag的同源率為75 88 ;蛋白質氨基酸序列的同源率為90 95 ,表明本研究確實克隆到了橡膠樹etri基因的cdna序列。 4By blasting the homologous sequences in genbank databases, the sequence of grass carp gh cdna from pituitary is 98 % homologous compared with the previously cloned gh cdna of grass carp. the cgh cdna fragment was inserted into pgex - 4t - l to construct the expression plasmid. the recombinant plasmid was digested by bamh i and ecor i to identify whether the cgh cdna fragment was inserted into the plasmid, the pgcgh was transformed into e. coli bl21 competent cells
將得到的序列在genbank和embl數據庫中進行了同源比較,結果顯示:本研究克隆到的草魚gh基因與genbank中登記的x60474草魚gh基因有12個堿基的差異,編碼的氨基酸有3個氨基酸殘基的差異,同源性為98 ,影響蛋白質高級結構的保守二硫鍵為2個。The nucleotide ( nt ) sequence of the insert in phz1754 is 2299bps in size. computer assisted analysis of the sequence revealed an open reading frame ( orf ) with a g + c content of 70. 3 % that would encode a protein of 552 amino acids ( aa ). the nt seque nce comparision revealed that the orf in the sequenced region exhibits 85 % dna sequence homology with the cholesterol oxidase gene choa of streptomyces sp
對phz1754進行外切核酸酶( exonuclease , exo )順序缺失,獲得單向長度漸減重疊的系列突變體,核苷酸序列測定顯示出該ecor - sal片段的精確大小為2299bps , frameplot程序分析揭示出該區域一個完整的開放閱讀框( orf )的存在,其大小為1656bps , g + c含量為70 . 3 ,編碼552個氨基酸,利用blastsearch程序將orf的核苷酸序列及推導的氨基酸序列與因特網上基因及蛋白質數據庫進行綜合比較,發現無論在核苷酸水平還是在蛋白水平上,該orf均與膽固醇氧化酶表現出同源性,而且與鏈黴菌膽固醇氧化酶同源性最高,說明該orf編碼膽固醇氧化酶基因。Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing
目的構建蛇毒鋸鱗蝰素( echistatin )的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素( echistatin )的氨基酸序列,結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性,設計了echistatin編碼基因,體外人工合成編碼基因dna片段,通過適當的限制性內切酶位點插入表達載體pbv220 ,分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆;進一步通過pcr技術構建echistatin的融合表達基因克隆。The close genetic relationship of goose parvoviruse and aav allows the examination of the molecular biological properties of the nonstructural proteins of gpv. after the gpv infected the cell the viral life cycle was regulated by the nonstructural proteins encoded by the virus. according to the published of gpv b strain genome nucleotide sequences in genbank and a pair of specific primers were disigned with oligo4. 1
本研究根據genbank發表的gpvb株全基因序列,藉助oligo4 . 1軟體設計一對引物,採用pcr技術擴增gpvh1株非結構蛋白ns2基因,並與pmd18 - t載體連接后測序,結果表明:鵝細小病毒h1株ns2基因核苷酸全長1356bp ,編碼451個氨基酸殘基,與gpvb株的ns2基因相比,核苷酸數目相同,有17個堿基、 6個氨基酸的差異;同源性分析表明:二者核苷酸序列同源性為98 . 75 ,推導氨基酸序列同源性為98 . 67 。The rest mutations in pp38 and pp24 are at random. sequence analysis also shows the first 195 nuclear acids of pp38 and pp24 are the same except for the 81 site ( g / c ), but this mutation does not cause the change of amino acid. we regard this as a genetic marker connecting with geography in the evolution of mdv but not related to isolated time and pathotype of different strain of mdv i
對pp24基因和pp38基因進行同源性比較分析的結果表明,絕大多數毒株二者的前195個核苷酸完全一致,不同毒株間的第81位核苷酸的差異( g / c )並不引起編碼的氨基酸變化,僅僅與地域分佈有關,這很可能是mdv在長期病毒衍化過程中形成的地域性遺傳標志,而與病毒的分離年代及mdv的致病型等因素無關。The mechanism of ki 11 ing the schistosomulae in vitro depending on the specific ige antibody were observed. the distribution of the protein encoded by the cloned gene was determined by immuno - electromicroscopy with protein a - colloidal gold. in order to further analyse the epitope features of the protein relevant to the specific ige antibody, the phage display library of random peptides was screened by pooled sera with the specific ige antibody
藉助現行生物信息學分析手段,通過網際網路進入genbank數據庫,對sj43b與已知序列進行同源性比對,應用dnatoois軟體對其編碼蛋白序列的氨基酸紐成、親水性、抗原性和可及性進行分析,並在blastp程序下在genbank蛋白數據庫中對sj43b編碼3人卞醫科大學博士學杠論文蛋白進行同源性檢索。Computer analysis of the cdna and its encoding protein the amino acid sequence encoded by full - length cdna is deduced by u - sing the blast procedure from ncbi. the homogeneous comparison and analysis are performed in the genbank and protein date house, while the functional residual sequence of the protein is analyzed in the molecular biological net, ht - tp : / / www
全長山na序列及其編碼蛋白質的計算機分析從cdna的序列推導出它所編碼的氨基酸序列,應用ncbi提供的blast程序在genbank數據庫和蛋白質數據庫進行同源性比較分析,在n tp : 。分享友人