酸性磷酸化酶 的英文怎麼說
中文拼音 [suānxìnglīnsuānhuà]
酸性磷酸化酶
英文
acid phosphorylase- 酸 : 酸構詞成分。
- 性 : Ⅰ名詞1 (性格) nature; character; disposition 2 (性能; 性質) property; quality 3 (性別) sex ...
- 磷 : 名詞[化學] phosphorus (15號元素,符號p)
- 酶 : 名詞[生物化學] (生物體的細胞產生的有機膠狀物質) enzyme; ferment
- 酸性 : [化學] acidity; acidness; acerbic; acidic property酸性材料 acid material; 酸性促進劑 acid acceler...
- 酸化 : acidate; acidulate; acidize; acidification酸化泵 acidizing pump; 酸化過程 acidization; 酸化劑 aci...
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According to the research of physiological and biochemical indicators or index, components of soluble proteins, substrate protein of phosphorylation and the activity of protein kinase in low - temperature stress in the leaves of brassica oleracea l., we tried to find the law of the physiological and biochemical response of brassica oleracea l. leaf to low temperature. at the same time, discussion on the signal transduction can also provide further evidences for revealing the mechanism of low - temperature stress. the results are showed as follows : malondialdehyde ( mda ), superoxide dismutase ( sod ), ascorbate peroxidase ( asp ) and peroxidase ( pod ) activities were changed greatly after 0 ~ 30min ' s treating with low temperature
本文以甘藍葉片為材料,通過對低溫5脅迫下甘藍生理生化指標、可溶性蛋白組分以及磷酸化底物蛋白、蛋白激酶活性的研究,以期找出甘藍葉片對低溫脅迫的生理生化響應規律,為甘藍露地越冬栽培防範寒害提供理論指導,同時對低溫脅迫下甘藍逆境信號傳導進行了探討,從而為徹底弄清低溫脅迫機理提供進一步的證據,研究的主要結果如下:丙二醛含量( mda ) 、超氧化物歧化酶( sod ) 、抗壞血酸過氧化物酶( asp )和過氧化物酶( pod )活性在低溫處理0 30min發生顯著變化,低溫處理3min后,甘藍葉片內mda含量基本沒有變化,處理5min時出現第一個峰值,達到對照的104 . 10 , 10min出現低谷,僅為對照的86 . 27 ,隨后再次上升, 30min時超過第一峰值,為對照的113 . 93 。Part 1 : the culture and identification of es - d3 cells and the study of the efficiency of eb formation from es cells when grown on mef feeder layer in es culture medium or cultured in es culture medium supplemented with lif 1000u / ml, es - d3 cells being used in our experiments formed normal clones, expressed akp and kept their normal karyotype over many passages. the in vitro and in vivo differentiation experiments showed that es - d3 cells could differentiate into variety of cell types derived from three primary germ layers
結果顯示: eso3細胞在小鼠胚胎成纖維細胞上和或含白血病抑制因於億f )的es細胞培養液中形成典型的胚胎幹細胞克隆,堿性磷酸酶染色結果為強陽性,具有正常二倍體核型以及具有在體內外分化為三個胚層來源的組織細胞的潛能,而且具有形成種系嵌合動物的能力。Alkaline phosphatase activity in gingival crevicular fluid during orthodontic tooth movement
正畸牙移動過程中齦溝液堿性磷酸酶活性的變化For uncovering the effects of reversible phospharylation on the structure and function of psii reaction centre, we purified a protein phosphatase associated membrane from the thykaloid membrane of ipomoea aquatica chloroplasts. in our experiments we studied the enzymology and spect rum characters of the purified phosphatase. in our lab, one kind of protein phosphatase associated thylakoid membrane of pomoea aquatica has been isolated
迄今人們對類囊體蛋白磷酸酯酶的研究較少,為了研究可逆磷酸化對psii反應中心結構與功能的影響,本文以蕹菜為材料,從葉綠體類囊體膜中分離純化到一種膜結合蛋白磷酸酯酶,進行了酶學性質和光譜性質的研究。Measured the protein content by lowery method and the activity of pkc by advanced takai method
蛋白激酶c是一種鈣活化的、磷脂依賴性的絲氨酸蘇氨酸激酶。Determination of alkaline phosphatase activity in milk ; enzymatic method
牛奶堿性磷酸酶活度的測定.酶活化法Chloroplast phosphoprotein were first found in thylakoid membranes by bennett o the attachment or removal of a phosphate group from a protein may have profound effects on that protein ' s activities and properties, the reversible phospharylation of the membrane protein in thykaloid is a dymanic equilibrium process
而bennett ( 1977 )發現的類囊體膜蛋白可逆磷酸化已成為近年研究的熱點之一,蛋白質連上或是移去磷酸基團都將強烈影響蛋白質的生理活性和性質,這需要類囊體膜蛋白激酶和磷酸酯酶的參與。The results showed with the application of coated compound fertilizer, the activities of soil urease, neutra phosphatase, catalase and sucrase were better than compound fertilizer treatment because of the significantly controlled release effects of the coated compound fertilizer
結果表明,在試驗條件下,由於包膜復合肥對養分的顯著控釋效果,它的施用對土壤脲酶、中性磷酸酶、過氧化氫酶和蔗糖酶活性的影響要好於普通肥料處理。The stations e2 and 1 - 4 were located at the cold water mass area of the central yellow sea, which characterized by low temperature, high salinity and stable theromocline would generate a retention mechanism that promoted the formation of separate, self - supporting stocks of krill. 2 genetic diversity and differentiation of p. latifrons specimens of p. latifrons were collected from the east china sea and the south china sea. the zymogram phenotypes of aspartate aminotransferase ( e. c. 2. 6. 1. 1, aat ), alkaline phosphatase ( e. c. 3. 1. 3. 1, alp ), a - amylase ( a - amy ), r - amylase ( r - amy ), esterase ( est ), lactate dehydrogenase ( ldh ), raalate dehydrogenase ( mdh ), malic enzyme ( me ), and phosphoglweoisomerase ( pgi ) were scored
(二)寬額假磷蝦遺傳多樣性和遺傳分化研究1 .本文對東海外海和南海2個站位寬額假磷蝦群體進行了分析,在檢測的9個酶系統中,共檢測到11個酶位點:天冬氨酸轉氨酶( l個位點, 2個等位基因) ,堿性磷酸酶( 2個位點, a加了和a加2各有2個等位基因) , r澱粉酶( l個位點, 2個等位基因) ,醋酶( 2個位點, es巧和est7各有2個等位基因) ,蘋果酸脫氫酶( l個位點, 3個等位基因) ,蘋果酸酶( l個位點, 2個等位基因) ,乳酸脫氫酶( l個位點, 4個等位基因) ,磷酸葡萄糖轉氨酶( l個位點, 3個等位基因) ; a澱粉酶為單態。Northern blot results show that nos. 66 - 1, 84, 89 - 1, 97, 108, 152, 175 and 233 have stronger signal in sp6 - tester than in sp6 - driver ; and no. 23 has weak signal only in sp6 - tester, nos. 94, 165, 172, 185 and 191 have similar hybridization signals in both sp6 - tester and sp6 - driver ; nos. 4, 17, 18, 28, 6 9, 101, 156 - 1, 157 - 1 and 183 do not reveal hybridization signals in both sp6 - tester and sp6 - driver ; the results of sequencing and blastn and blastx on ncbi indicate that no. 23 cdna ( 846bp ) has significant alignments with nicotiana tabacum mrna for elicitor inducible beta - 1 - glucanase nt - sube76, and arabidopsis thaliana clone 7119 for glycosyl hydrolase family 17 ( protein id : at5g55180. 1, supported by cdna : 7119, supported by cdna : gi _ l 87001 54 ) and arabidopsis thaliana beta - 1 - glucanase - like protein ( gi _ 2 1594590 ) ; no. 84 cdna ( 560bp ) has significant alignment with lotus corniculatus aspartate aminotransferase mrna ( complete cds length = 1685, gi | 2605931 | gb | af029898. 1 | af029898 ) for aspartate aminotransferase ; no. 89 - 1 cdna has significant alignment with arabidopsis tha
與同源性最高的擬南芥類似晚期胚胎發生高豐度蛋白比較,二者都具有lea 2結構域、保守分泌蛋白cog5608結構域和低復雜度區,都具有pkc磷酸化位點、酪蛋白激酶磷酸化位點、 n十四酞化位點和酚胺化位點,所不同的是: ( )在結構功能域上, 152全長cdna編碼的蛋白質序列中多了1個lea 2結構域、 l個保守分泌蛋白cog5608結構域和1個低復雜度區; ( 2 )在功能位點上, 152全長cdna編碼的蛋白質具有酪氨酸硫酸化位點、多了l個酪氨酸激酶磷酸化位點和1個可能的天冬氨酸富集區,但沒有n糖基化位點; ( 3 )擬南芥類似晚期胚胎發生高豐度蛋白的lea 2結構域具有顯著性( eThere was no difference in other biologic characteristic of mscs between the two separation method, such as cell anchorage ratio and clone formation ratio. ( 2 ) plga film presented uniformity frame with no protuberance and fissure under scanning electron microscopy ( sem ). big aperture with smooth wall and average 400 m i n size running - through each other was observed in porous plga substrate, around the big aperture there were many round micropores about 5 m size. all of the structure were equal and uniform, which satisfied the further research work. ( 3 ) mscs adhesion at earlier time was promoted by biotiegenrafter 3h, cell number was ( 1. 5 0. 18 ) 105 in the plga film coated with biotiegen group, which was significantly higher than that in plga film group ( p < 0. 01 ) and higher than that in coverslip group ( p < 0. 05 ), which cell number was ( 1. 04 0. 21 ) 105. after 6h and 12h biotiegen could not promote cell adhesion, and cell proliferation and alkaline phosphatase ( alp ) activity were not promoted dramatically during 9 days. ( 4 ) cell adhesion was promoted by fibronectin or collagen type i
G ) i型膠原、纖維粘連蛋白促進細胞增殖,細胞接種后3 、 6 、 gd三個檢測時間點,實驗組細胞均明顯高於對照組。與1型膠原相比,纖維粘連蛋白刺激作用更強。 ) i型膠原、纖維粘連蛋白尚能誘導mscs細胞向成骨細胞分化,不僅表達成骨細胞標志物ocn 、 alp 、 opnmrna ,而且堿性磷酸酶活性明顯增高,堿性磷酸酶及鈣結節7第四軍醫大學博士學位論文一染色均強陽性, i型膠原組mscs細胞堿性磷酸酶活性較fn組更高,有顯著性差異;同時,兔疫組化染色表明,經纖維粘連蛋白作用的mscs1型膠原表達陽性。The cyst cells enclosing spermatomeres maybe synthesize a kind of scf - like protein, which can recognize specially the c - kit receptor on the cellular membrane of spermatomeres. then c - kit is activated, dimerizing and autophosphorylating. at the same time, the tyrosine kinase domain of c - kit is activized, which phosphorylates the proteins that have sh2 domain
精母細胞周圍的囊細胞可能合成scf樣蛋白,特異地識別精母細胞膜上的c - kit受體,並刺激c - kit發生二聚化、自體磷酸化,激活胞內酪氨酸激酶活性,活化具有「 sh _ 2結構域」的靶蛋白,可能通過一系列信號級聯,最終激活與減數分裂的相關蛋白或基因。Methods the histochemical staining of alkaline phosphatase was used to show microvessel of human body s flap
方法應用酶組化法對人體皮瓣的微血管進行鹼性磷酸酶染色。The rnapii - associated complex elongator has histone acetyltransferase ( hat ) activity and thus might fulfil such a role
與磷酸化的rnap結合的elongator復合物具有組蛋白乙酰轉移酶( hat )活性,它可能執行這一功能。Soil microbial biomass carbon, microbial biomass nitrogen, soil basal respiration and metabolic quotient all showed a decreasing trend under more than 15 mg. kg - 1 cadmium or more than 200 mg. kg - 1 lead, respectively. soil dehydrogenase, urease and acid phosphotase activities have a significant decrease, and the activities of soil catalase and invertase taking sencond place, while soil protease activities showed stable state
隨重金屬濃度增加,各指標下降幅度各有差別,其中微生物生物量碳、微生物生物量氮以及基礎呼吸和微生物代謝商隨重金屬濃度增加而明顯下降;土壤脫氫酶、脲酶、酸性磷酸酶活性的下降幅度較為明顯,過氧化氫酶、蔗糖酶活性次之,蛋白酶活性較為穩定。The soil phosphate activities were determined after 0, 10, 50, 60 days. the results show that alkaline phosphate activity was degressive, acidic phosphate activity changed inconsistently and neutral phosphate activity was ascending slowing. the analyse of the phosphate activity ' s absolute change also shows that the influence of soil algae on phosphate activity is not prominent
結果表明,待測土壤中的堿性磷酸酶活性全部是下降的,中性磷酸酶活性緩』陛上升,而酸性磷酸酶活性的變化沒有太大的規律性,但是總體上還是上升的、其磷酸酶活性絕對變化的方差分析也表明藻類對土壤磷酸酶的影晌不顯著。The paper studied three aspects of extracelluar enzymes in sediments of the tidal flat wetland, namely 1 ) the distibution of five sorts of extracellular enzymes in sediments in the east end of chongming island along the elevation gradient or community succession series, the relationships between the activities of enzymes and the ecological factors, and functions of extracellular enzymes in the process of community succession ; 2 ) the effects of the heavy metal ions and edta on the activity of alkaline phosphatase in sediments of the east end of chongming island by adding and removing of heavy metal ions, discussing whether the activities of extracellular enzymes could be taken as the indicators for the environmental status ; 3 ) the variations of the activities of extracellular enzymes in sediments in the east end of hengsha island after the discarding clay
本文以長江口典型濕地?崇明東灘為例,首次研究了沿高程梯度或沿植被演替系列沉積物中堿性磷酸酶等五種胞外酶活性的空間分佈規律,分析了胞外酶活性與環境因子的相互關系及其產生機制,討論了胞外酶活性在濕地植被演替中的作用。同時以崇明東灘沉積物為對象,運用重金屬離子的添加和去除等方法,研究了重金屬離子對沉積物中堿性磷酸酶活性的影響,利用胞外酶活性的變化探討了崇明東灘重金屬污染的狀況。此外,本文還研究了橫沙東灘吹泥試驗工程對沉積物環境因子和胞外酶活性的影響並進行了對比分析。Results the study had revealed that not only the bone tubular and the degree of mineralization, but also the activity expression of alkaline phosphatase of osteoblasts in fluoride group were better than the control group
結果研究發現氟組成骨細胞不僅其礦化結節和礦化程度,而且其堿性磷酸酶活性表達都要早於對照組。This method has several strong points : ( 1 ) eliminating the possibility of ringing self of vector. ( 2 ) the inserting fragments ca n ' t ligate each other. ( 3 ) the translating rate with the partially filled in method is equal to phosphatase method
末端半補齊技術的優點有: ( 1 )徹底消除載體自環化的可能性; ( 2 )消除了插入片段自身相互連接的可能性; ( 3 )同常用的堿性磷酸酯酶法相比較,採用半補齊技術其連接產物的轉化率不受影響。One is phosphatase - like domain and the other is glycerol - 3 - phosphate dehydrogenase domain. the former domain may catalog the dephosphation of the glycerol - 3 - phosphate and the later presumably synthesize the glycerol - 3 - phosphate according to similarity searching, domain localization and structure comparison. these should give an evidence for the functional research of this gene
通過相似性搜索,結構域定位以及結構比較分析我們認為磷酸化酶結構域很可能是催化3一磷酸甘油水解,而3一磷酸甘油脫氫酶結構域是催化3一磷酸甘油合成的,為這個基因功能的鑒定提供了根據分享友人