重組位點 的英文怎麼說

中文拼音 [zhòngzǔwèidiǎn]
重組位點 英文
recombination site
  • 重 : 重Ⅰ名詞（重量; 分量） weight Ⅱ動詞（重視） lay [place put] stress on; place value upon; attach im...
  • 組 : Ⅰ名詞1 （由不多的人員組成的單位） group 2 （姓氏） a surname Ⅱ動詞（組織） organize; form Ⅲ量詞（...
  • 位 : Ⅰ名詞1 （所在或所佔的地方） place; location 2 （職位; 地位） position; post; status 3 （特指皇帝...
  • 點 : Ⅰ名詞1 （液體的小滴） drop （of liquid） 2 （細小的痕跡） spot; dot; speck 3 （漢字的筆畫「、」）...
  • 重組 : bpr
  1. Finally 4 right clones was obtained from 864 transformants by pcr detection. sequencing analysis showed that the hsa gene have been introduced into the right position of the human a - lactalbumin yac. furthermore, works to optimize the conditions of introducing the recombinant yac into goat f ibroblast via cell fusion was explorded

    經pcr檢測，從864個轉化子中獲得了4個陽性克隆，測序表明，人血清白蛋白基因已正確的導入到人-乳白蛋白基因yac的特定位點上，並獲得了可進行細胞融合的重組yac載體。
  2. The fusion protein was bactericidal active against staphylococcus aureus. in present study, we will truncate the none channel - forming do main, then attach the agrd to the pore - forming region ( k544 - i626 ) to construct a new engineered multidqmain protein machine - compact engineered peptide targeting staphylococcus aureus. such engineered peptide was constructed by linking the gene of staphylococcal agrd pheromone with the gene of c - terminal ( 1626 ) of colicin la pore - forming region ( k544 - i626 ) with site - directed mutation

    利用點突變方法將金黃色葡萄球菌信息素agrd （ i型， ystcdfim ）的基因引入到大腸菌素fa梭基端1626基因上，並將限制性內切酶sacl酶切位點基因分別引入到大腸菌素fa的p4和k544上，通過酶切、膠回收、連接獲得含大腸菌素ia水性孔道結構域和金黃色葡萄球菌信息素agrd基因的重組質粒。
  3. It is inferred that its active transcription occurs in the same region, not the nucleoplasm. the result will help us to further comprehend the mechanism of rna polymerase transcription, the way of its transcripts processing and transport, and the structural and functional relationship among the three rna polymeraes

    這一結果不僅直觀地向人們表明了rna聚合酶在真核細胞核中的轉錄位點，而且對於人們進一步認識和理解rna聚合酶的轉錄機制、其轉錄產物的加工運輸途徑、以及真核細胞當中不同的rna聚合酶間的組織和調控關系都將有著重要的理論意義。
  4. The recombinant expression plasmids prt - p450nor and pet - p450nor were constructed by inserting p450nor gene into the bamh i / hind iii site of the prokaryotic expression vector prset and pet28, then they were transformed into e. coli bl21

    本研究將已克隆的真菌細胞色素p450nor基因插入原核表達質粒載體prset和pet28的bamhi / hind位點，成功構建重組表達質粒prt - p450nor和pet - p450nor ，並轉化到e . colibl21 。
  5. 4. engineering dhqase ( arod ) - deficient e. coli mutant with a second copy of the arob gene gene targeting technique was used to disrupt the arod gene in e. coli chromosome. the mutant 31bk was engineered, in which homologous recombination of the arobkanr gene cassette into the arod locus ( arod : : arobkanr ) of the e. coli strain atcc31884 genome utilized the helper plasmid pkd46 with red system. the host cell 31bk lacked catalytic activity of dhqase ( arod ) and had a second copy of the arob gene, so it improved carbon flow into the quinic acid biosynthesis direction

    構建宿主菌基因精確定位突變株31bk （ arod : : arobkan ~ r ）為了改變代謝途徑脫氫奎尼酸（ dhq ）分支點上的代謝流量，使之充分流向目的產物奎尼酸合成方向，利用基因打靶技術構建了31884宿主菌arod基因精確定位插入突變體，使dhq脫水酶（ dhqase ）失活，阻斷了碳代謝流流向芳香氨基酸生成的方向，同時用同源重組的方法將arob基因定位整合入染色體上，解除了限速酶對碳代謝流通過共同途徑到達dhq的阻遏影響，並減輕代謝負擔。
  6. Melatonin ( n - acetyl - 5 - methoxytryptamine ) was discovered by a lerner and his colleagues [ l ] as the principal hormone of vertebrate pineal gland, an unpaired appendage of the brain in most vertebrates and named by him after its effects on amphibian melanophore. however, it is now known that many cells and tissues synthesis and the nature of organisms, melatonin is secreted in the night, thus, melatonin is identified as an endocreine index of the darkness and referred to as darkness hormone [ 3 ]. melatonin has aslo been called by other names, such as nature ' s sleeping pill for it role in sleep - wake cycles, and circadian glue for its increasing importance in the regulation of circadian and circannual physiological and behavioural functions, moreover it is also involved in the regulation of the immune respone and various cns activies, in the last decade, considerable attention has been devoted to the pharmacology of melatonin in view of its potential applications in various therapeutic areas

    褪黑素（ n -乙酰- 5 -甲氧基色胺， melatonin ，簡稱mt ） ，由lerner和他的同事們發現[ 1 ] ，后來根據它對兩棲動物黑色素細胞的影響而命名，是脊椎動物松果腺分泌的一種重要激素。松果體在大多數多脊椎動物中是一個孤立的附屬器官。然而，現在眾所周知很多細胞和組織合成mt ，若不考慮合成的位點和機體的屬性， mt在晚上分泌，正因如此， mt被認為是一種黑暗的內源性指標，被稱作黑色激素[ 3 ] 。
  7. Thirdly, after researching state - owned enterprises " industrial distribution both in china and abroad, indicates the targets of state - owned enterprises " industrial reorganization are to form reasonable scale and industrial distribution, to enhance the " state - owned economy ' s dominant function, to vivify the industrial organization and to enhance the competition ability of industry

    第三部分，國有企業產業重組的目標和重點。通過對市場經濟國家國有企業產業定位的分析，指出我國國有企業的產業定位與市場經濟國家具有一致性，提出國有企業產業重組的目標是形成國有經濟的合理規模和產業分佈，增強國有經濟的主導作用，優化產業組織、提升產業競爭力。
  8. The recombinant transfer vector pbacpak - hbmp was constructed by insertion of the hbmp coding sequences into the multiple cloning site of transfer vector pbacpak. 8. bmn cell line was co - transfected with pbacpak - hbmp plasmid and linearized baculovirus bacpak6 dna by dosper agent

    將克隆到的hbmp基因通過適當的酶切插入到轉移載體質粒pbac - pak8的多克隆位點中，獲得重組轉移載體質粒pbacpak - hbmp 。
  9. Vp3 gene of hl isolate of goose parvovirus derived from recombinant piasmid pproex htb - vp3 was subcloned into ecorl site of psy538, and the reporter gene lacz with promoter pll was cloned into smai site of recombinant piasmid. both vp3 gene and lacz gene were cloned into noti site of psy681, recombinan fpv transfer vector containing vp3 gene of gpv was obtained. the result is basis of construction of recombinant fpv expressing vp3 gene of gpv and gpv genetic engineering vaccine

    本研究另從含有gpvh1分離株主要結構蛋白vp3基因的重組質粒pproexhtb - vp3中切取gpvh1株vp3基因片段，將其亞克隆于psy538的ecori位點，然後將帶有痘苗病毒啟動子p11的lacz報告基因平端克隆于上述重組子的smai位點，再用noti切下同時含有vp3基因和lacz報告基因的片段，再亞克隆于psy681的noti位點，構建出含有vp3基因的重組禽痘病毒轉移載體，為構建表達vp3基因的重組禽痘病毒從而制備gpv基因工程疫苗奠定了基礎。
  10. In rescent 10 vears in our counrtry, some excllent enterprises, such as hair, also used this instrument and achived a great success. this is a good example for zigong cemented carbide co. ltd. but zigong cemented carbide co. ltd has its own character, and has to be considered its own specialty and its location when using capital instrument. only when the company connect these two sides organically, then the realistic strategy target can be drawn up and the developing location can be chosen. under guidline of the detailed mergence. purchasing, reorganization and investment ' s policy with hard effort and work, the zigong cemented carbide co. ltd future should be glorious and the company ' s developing t - arget should come true

    但是，自貢硬質合金有限責任公司有其特殊性，在運用兼并、收購、重組、投資等資本運營手段時要考慮自身特點、公司地理，要考慮資本運營手段的原則、特點的方法，要考慮創造性地將兩者有機結合起來，制訂出更加切實可行的戰略目標，選準定位，並在具體的兼并、收購、重組和投資策略指導下，奮發努力，扎實工作，這樣，自貢硬質合金有限責任公司的前景必將更加美好，公司的發展目標必將得到實現。
  11. Thus, immunologists have sought smaller molecules with the antigen - recognition capability of antibodies. the variable domains of the heavy ( h ) and light ( l ) chains are sufficient for antigen recognition but the non - covalent complex of the two variable domains ( fv ) is unstable. it is possible to genetically engineer a single - chain fv ( scfv ) with the h chain v region connected to the l chain v region via a 15 amino acid linker composed of serine and glycine amino acid residues

    二、日本血吸蟲單克隆杭獨特型抗體np30的單鏈狐（ scf ）的構建、表達及對balbic小鼠誘導保護性作用研究l 、日本血吸蟲單克隆杭獨特型抗體np0的單鏈抗體歸cfv ）的構建、表達通過pcr方法體外擴增並經測序驗證的重鏈、輕鏈可變區（ vh 、 vl ）基因先後重組入原核表達質粒ptha90相應的位點上，中間通過一連接肽（ gly在er ） 。
  12. The recombinant vector was digested with tthllll and the lacz gene from e. coli was inseted in this site, the generated plasmid is designated as pltk - uni

    然後定向亞克隆swha基因於多克隆位點，獲得重組轉移載體pltk - ha 。
  13. This new approach to tissue engineering of the maxillofacial region would be advantageous because of its site - specific anatomical configuration as well as its potential ability to adapt to the functional forces placed on it during function

    這種頜面部組織工程新技術具有優點，一方面符合重建位的解剖形態；另一方面能夠在行使功能時，適應功能力量而發生改建。
  14. Therefore, blys, its receptor or related antagonists may find medical utility in the treatment of b cell disorders associated with autoimmunity, neoplasia, or immunodeficiency syndromes. in this study, epo signal peptide sequence and hsblys gene were linked by soe method. the fusion product was cloned into eukaryotic plasmids. pcdna3, pcdna3. 1, pefneo, respectively. meanwhile, the epo signal peptide sequence was mutated so as to form a restriction enzyme cut site : bin i. thus the recombinant plasmid can be used as secreting plasmid expressing other gene

    本實驗通過3 』端互補，進行引物延伸合成epo信號肽序列：信號肽和hsblys基因採用重疊延伸拼接法形成融合基因；融合基因分別插入pcdna3 . 0 、 pcdna3 . 1 、 pefneo真核載體：引物延伸合成信號肽時，利用亮氨酸同義密碼，將信號肽基因的倒數第二個密碼突變，在重組載體上的信號肽序列之後，形成bln酶切位點，使三種載體成為分泌表達載體。
  15. Based on a 3. 1kb pst i fragment of genomic dna of a wild s. avermitilis, a 1. 5 kb apramycin resistance fragment was inserted into sph i site of avec gene in the 3. 1 kb fragment, then a recombinant plasmid pc05 was obtained by introducing above inactivated avec fragment into mcs region of phjl401. competent cells of et12567 were transformed by recombinant plasmid pid03 and pc05 respectively

    以含有avec基因的3 . 1kb基因組dnapsti片段為基礎，將1 . 5kb的安普黴素抗性基因片段插入到avec基因中的sphi酶切位點，再將此插入失活的avec基因片段連接到具有接合轉移功能（含有orit基因）的鏈黴菌-大腸桿菌穿梭質粒phjl401的多克隆位點區，由此得到重組質粒pc05 。
  16. In this paper, grouped weight matrix ( gwm ) method is proposed for splice site recognition

    摘要提出識別剪切位點的分組權重矩陣方法。
  17. As well as in eukaryocyte ( hepg2 and cos - 7 ), then detect their antigenity as a basis study and explore of the choice of immunogen for preventive and therapeutic vaccines of hepatitis b. methods : the gene fragments coding 152aa ( si ) and 124aa ( s2 ) of the carboxyl terminus of hbsag were amplified by pcr from plasmid pecob6 with a pair of primers containing different endonuclease sites and were cloned into multiple cloning sites of plasmid pbks ( + )

    為乙型肝炎的預防和治療性疫苗免疫原的選擇進行初步的研究和探討。方法：本研究利用聚合酶鏈反應（ pcr ） ，通過設計帶有不同酶切位點的一對引物，從質粒pecob6特異性擴增hbsag蛋白羧基末端152個氨基酸（ s1 ）和124個氨基酸（ s2 ）的基因片段，分別將二者克隆到質粒pbks （ + ）的多克隆位點，篩選重組克隆。
  18. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
  19. Ruian bafang sewing equipment co., ltd. is a joint - stock enterprise through enforcing property right recombination in 1998 from ruian industrial sewing machine general factory - which is a key government - owned enterprise and a municipal civilized unit of ruian

    年由瑞安市級文明單位市重點國有企業瑞安市工業縫紉機總廠實施產權重組改制的股份企業。屬中國縫紉機協會成員單位。
  20. Methods the 54th generation of transformed human embryonic tendon cells and artificial composite materials of carbon fibers ( cf ) and polyglycolic ( pga ) were co - cultured in vitro to construct tet. lt was frozen in liquid nitrogen with four kinds of cpa for 2 months. post - thawed quickly and transplanted into hind limbs of nude mice, and repaired the defects of achilles tendon. after 2, 4, 6, 8, 12 weeks, the morphological, histological, ultrastructure, short tandem repeat loci and immunohistochemistry examination were detected, and biomechanical strength of tet were examined. result tendon cell survived and could secret type i collagen after 12 weeks to transplanted into nude mice. in the group of dmso + raffmose + kh2o4, vacuole in mitochondrion degraded i tendon cell ranged in order, abundant collagen fibers were found and linked each other and the biomechanical strength was increased as time elapsed. c onclusion dmso + raffmose + kh2o4 could protect tet in deep low temperature

    組織工程肌腱制備完成後在四種抗凍劑保護下液氮凍存2月；快速復溫后植入裸鼠以修復跟腱缺損， 2 、 4 、 6 、 8 、 12周后取出，觀察形態學、組織學、電鏡和免疫組織化學變化，短串聯重復位點檢測和生物力學變化。結果實驗組組織工程肌腱體內植入12周后仍有肌腱細胞存活並分泌型膠原；隨著時間延長， 10二甲基亞碸（ dmso ） +棉子糖（ 30mmol l ） + kh _ 2po _ 4 （ 25mmol l ）組線粒體空泡減少，肌腱細胞排列整齊，膠原纖維增粗並連接，抗拉強度增高。
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