重組劑 的英文怎麼說
中文拼音 [zhòngzǔjì]
重組劑
英文
recombinagen-
Porcine transmissible gastroenteristis is an importan contagious disease endangering the development of swine. in other to establish a rapid diagnosis method and provide effective immunogenic products, the nucleoprotein ( n ) gene of porcine transmissible gastroenteristis virus ( tgev ) was cloned. expressed and its expressed product was purified
為建立對豬傳染性胃腸炎快速有效的診斷方法，並試圖在預防上提供有效的免疫制劑，本論文首次在我國對豬傳染性胃腸炎病毒核衣殼蛋白基因進行了克隆、鑒定、表達及重組核蛋白的純化；並在細胞上對重組核衣殼蛋白抗體的中和效力進行了測定。In this paper, the studies on breeding aspect, energy level and feed nutrition, including exogenous porcine somatotropin ( pst ), - incitant, l - novain, lycine, rucca schidigera and electrolyte, which would affect the pork quality were also reviewed
本文從飼養狀況、飼糧熱能水平、飼糧營養成分（包括蛋白質和氨基酸、脂肪、礦物元素和維生素） 、生長促進劑（包括重組豬生長激素、 -興奮劑、 l -肉堿、甜菜堿、莫哈夫絲蘭提取物和礦物元素）等方面對豬肉品質影響的研究進展做了綜述。The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides
進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。Studies of the crystal structure of endostatin have shown a compact globular fold, with one face particularly rich in arginine residues acting as a heparin - binding epitope, this site was recently shown to be involved in the inhibition of induced angiogenesis. experimental studies show that recombinant endostatin specifically inhibits the proliferation of endothelial cells in a dosedependent fashion. recombinant endostatin from bacteria is largely insoluble, but still efficient in arresting tu mor growth after injection into mice. intermittent therapy with recombinant bacterially produced endostatin reduces several experimental tumors, including lewis lung carcinoma, to a dormant state. no sign of drug induced resistance has been reported and, in the original study, the treatment dormancy appeared to persist even when therapy was discontinued. sowe regard endostatin as a promising anti - tumor drug
許多研究表明重組內皮抑素特異性抑制內皮細胞增殖，而且這種抑制作用呈劑量依賴性。細菌表達產物內皮抑素大部分以不溶形式存在，將這種混懸液注射治療老鼠仍可以抑制腫瘤生長。于小鼠皮下重復注射內皮抑素重組蛋白，幾乎完全抑制鼠lewis肺癌等多種腫瘤生長，並無耐藥性產生，即使中斷治療腫瘤也不再復發。Goat anti - human ige antibody were used as second antibody to make sure that the positive clones were ige related. through three cycles of screening, the inserted cdna fragments of the positive clones were amplified by pcr and sequenced. the results showed that the inserted cdna fragment from one clone was 1200 bp in length, with a orf of 507 bp which encoded 169 amino acids
Sj43b pgex 6p 1重組質粒的誘導表達、表達產物的鄉寸和免疫學性質鑒定分析為獲得可溶性的rsj43b月6gsta蟲合蛋白，對不同iptg誘導劑濃度、誘導表達溫度和誘導表達時間等因素對融合蛋白可溶性表達的影響進行了觀察。Total rna was extracted from the second stage larve of hypoderma sp, then single chain cdna was synthesized by reverse transcription using oligo ( dt ) 18 as a primer. the hypodermin c ( hc ) and hypodermin a ( ha ) gene specific primers were devised by dnastar software
本試驗的目的旨在進行hypodeminc （ hc ）和hypodermina （ ha ）基因的克隆、測序、構建重組表達載體並誘導表達，獲得重組抗原，以解決天然抗原的不足並為診斷和免疫試劑的產業化奠定基礎。In our laboratory, a unique mutation detection system using a shuttle vector plasmid has been established to demonstrate that a low concentration of mnng ( 0. 2 m ) can induce nontargeted mutation in mammalian cells : the mammalian cells were exposed to 0. 2m mnng for 2. 5h, then a shuttle plasmid pz189 carrying supf trna gene was transfected into cells after 24h culture. we found a 5 - fold higher mutation frequency of the plasmid replicated in pretreated cells than the spontaneous mutation frequency of the plasmid replicated in control cells. this kind of mutation did not occur immediately after mnng exposure
我們實驗室曾用一特殊的突變檢測系統，直接證明dna損傷劑可在哺乳動物細胞誘發非定標性突變：首先用低濃度（ 0 . 2 m ）的短壽烷化劑mnng （半壽期為1 . 1hr ）處理細胞2 . 5h后，繼續培養24h ，將重組有用作突變檢測的靶基因supftrna基因的穿梭質粒pz189轉入細胞復制，發現在未受致癌物直接攻擊的穿梭質粒中有較自發突變率高5倍以上的靶基因突變。The research interests of this group include : aborvirus diagnosis technology development and the interaction of aborvirus and mosquitoes, entomopathogenic bacteria and insecticidal gene resources, microbial genomics and comparative genomics, insecticidal proteins and their mode of action, construction of engineering strains with higher toxicity and wider active spectrum, production, standardization and the application of bio - pesticide and other microbial agents, resistance mechanism in target insects and the resistance management
重點研究登革熱病毒、乙型腦炎病毒和西尼羅病毒的快速檢測及病毒與宿主的相互作用關系，蚊蟲病原微生物菌種及其基因資源，微生物基因組學和比較基因組學，殺蚊毒素蛋白特性和作用方式、殺蚊細菌的遺傳改良和工程菌株的構建，新型細菌殺蚊制劑的研製及野生型和重組微生物對環境的安全性評估等，發展新的生物防治技術，建立和完善以生物防治為主的蟲媒病毒媒介蚊蟲綜合防治體系。In the third part of the thesis, a chlamydomonas reinhardtii chloroplast expression vector, pactbvpl, containing the fusion of the foot and mouth disease virus ( fmdv ) vp1 gene and the cholera toxin b subunit ( ctb ) gene was constructed. transformation of c. reinhardtii chloroplast was achieved by biolistic bombardment with pactbvpl
論文第三部分主要敘述了將o型fmdvvp1與強黏膜免疫佐劑霍亂毒素b亞基（ ctb ）的融合基因克隆重組到衣藻葉綠體表達載體中，並採用基因槍法轉化衣藻葉綠體，獲得了具有壯觀黴素抗性的轉化子。In order to develop recombinant poifn - a preparations preventing porcine viral infectious disease, and to further expound the research of cytokines in the field of molecular biology, mature poifn - a was cloned and expressed in e. coli expression system in the study
為了在我國研製生物工程重組豬干擾素類制劑，防制豬病毒性傳染病和進一步開展豬干擾素分子生物學研究，本文進行了豬干擾素基因（ poifn ）的克隆、表達及其重組蛋白抗豬瘟病毒的研究。Monitoring insulin in procedure of production and purification, determining insulin content from bio - tissue fluid and single islets of langerhans, and studying insulin molecular structure are all dependent on chromatographic analysis
胰島素制劑的純度檢驗，胰島素生產及純化過程的監控，生物體液及胰島細胞分泌胰島素的狀況，重組胰島素結構的剛定及活性研究等都離不開胰島素的測定。2. construction of chimeric mtb8. 4 / hil - 12 eukaryotic expression plasmid ( 1 ) construction of pci - neo - mtb8. 4 - linker ( pml ) and pci - neo - ms - linker ( pmsl ) mtb8. 4 - linker and ms - linker gene ( without stop codon ) were pcr amplified by using two oligonucleotides designed to generate nhe i and mlu i restriction sites at the 5 " and 3 " ends of the amplified fragments, respectively
3 .重組質粒在真核細胞中的表達: pm 、 pms 、 pmi和pmsl重組質粒用lipofectaminatmzo0o脂質體轉染試劑轉染cos一7細胞，進行瞬時表達， 48小時后，用rl 』 - pcr檢測目的基因在mrna水平的表達;用westemblotting檢測hil一12在蛋白質水平的表達。Expressed sequence tag ( est ) analysis was carried out to study the molecular mechanism of salt tolerance for polygonum sibiricum laxm. a cdna library was constructed from polygonum sibiricum laxm. treated by salt using stratagene cdna synthesis & gigapack iii gold cloning kit
為了研究西伯利亞蓼耐鹽的分子機理，使用stratagene的cdna合成和gigapack gold包裝試劑盒構建了鹽（ nahco _ 3 ）脅迫下西伯利亞蓼的cdna文庫，隨機選取重組克隆測序，用表達序列標簽（ est ）方法研究鹽脅迫下西伯利亞蓼基因的表達。Huctla4 - lg, " one of the most potent in1n1unosuppressive molecules, is a soluble recombinant fusion protein ( molecu1ar weight of approximately 92 kd ) consisting of the extracellular domain of human ctla - 4 and a fragment ( hinge and constant region ) of the fc portion of human iggl. it strongly adheres to the b7 molecule to block the cd28 - mediated costimulatory signal, resulting in inhibition of in vitro and in vivo immune response
可溶性重組融合蛋白huctla4 - ig是一種新穎的免疫抑制劑，分子量約為92kd ，由人ctla - 4分子的胞膜外區與人igg1的fc段（鉸鏈區與恆定區）融合形成，能牢固地與b7分子結合，阻斷cd28介導的共刺激信號，導致免疫反應的抑制。Product this adopt newest hair care fill a prescription, include ppt high energy water raise factor, silk albumen factor, hair softener, etc. various kinds of hair necessary high energy fertilizer, choosing the most popular color, the overall reorganization is repaired to breaking out beautifully while having hair dyed, let the beautiful hair keep permanent and long healthy and beautiful color
本品採用最新護發配方，內含ppt高能水養因子、絲蛋白素、頭發柔軟劑等各種毛發所需高能肥料，選擇最流行的色彩，在染發的同時對秀發作全面的重組修復，讓秀發保持恆久的健康及亮麗的色彩。Ii ) two fragments ( about 240bp and 130bp ) were amplified from human keratinocytes total rna by rt - pcr. the recombinant pm - hpabl and pm - hpabs plasmids were constructed by inserting 240bp and 130bp pcr products into pmd 18 - t vector, respectively. the recombinants were identified by restriction enzyme analysis and dna sequencing, iii ) two orfs ( > 100bp ) were found in the insert sequence of pm - hpabl
應用smartpcr試劑盒和簡並引物從人皮膚角質形成細胞cdna中擴增到長分別為24obp和13obp左右的2種片段，將它們插入pmd18一t載體，用酶切法初步篩選陽性重組子pm . hrabl和pm一hrabs ，對陽性重組子進一步作測序鑒定。Methods ; rt - pcr method was used to amplify the coding sequence of sh2a gene. eukaryotic recombined expression vector, pcdnas. 1 - sh2a was constructed and then transfected bel7402 cell and cos
細胞激酶活性測定相關試劑二、實驗方法通過rt pcr方法擴增shzacdna編碼序列，構建真核重組表達載體pcdna3Applications of animal ' s growth hormone are mostly studied by means of protein type, up to now, no other reports about gh gene directly used in animals have been found except one paper about the transfection of human gh gene into mice. in this research, we studied the changes in the bullfrogs after they are separately injected with the recombinant bullfrog gh protein ( re - bfgh ), bullfrog gh plasmid ( vb / gh ), grass carp gh plasmid ( vgcgh ) and expression vector vr1020 while the 0. 85 % salt - water as the control, for the purpose to determine the possibility of that the eukaryotic expression plasmid vbfgh and vgcgh are expressed in adult bullfrogs and affect their growth rate and plasma gh. we hope the results will help developing a new approach to promote the animal growth
本研究首次以兩棲動物牛蛙為研究對象，進行了其生長激素基因的克隆以及原核表達質粒與真核表達重組質粒的構建、重組牛蛙gh蛋白的生物活性和免疫活性檢測以及重組蛋白制劑和核酸制劑的制備及其體內促長作用等表達效應研究，研究目的在於求證重組真核表達質粒是否能在牛蛙中表達、其促長效應是否強于重組bfgh蛋白，為探索重組生長激素真核表達質粒能否替代重組gh蛋白作為動物促長基因制劑等研究奠定理論基礎。Two kinds of conditions influence the humoral immunomodulation induced by emotional stress
重組人內抑素對佐劑性關節炎大鼠滑膜組織血管內皮細胞生長因子表達的影響The deletion mutants were proved to be genetically stable, and thus might be promising strains in industrial production of avermectins b. although s. avermitilis produces eight related components of avermectins, only two components, bla and bib, are available for the medical, veterinary and agricultural fields
該缺失突變是在染色體上通過同源雙交換完成的，不會發生進一步的重組，因此突變株性狀穩定，在工業生產上具有應用價值。阿維菌素的天然發酵產物共有八個組分，其中只有b1組分的殺蟲活性最高，被作為殺蟲劑在農業和畜牧業中使用。分享友人