重鏈可變區 的英文怎麼說
中文拼音 [zhòngliànkěbiànqū]
重鏈可變區
英文
variable region of heavy chain-
Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )
Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。Thus, immunologists have sought smaller molecules with the antigen - recognition capability of antibodies. the variable domains of the heavy ( h ) and light ( l ) chains are sufficient for antigen recognition but the non - covalent complex of the two variable domains ( fv ) is unstable. it is possible to genetically engineer a single - chain fv ( scfv ) with the h chain v region connected to the l chain v region via a 15 amino acid linker composed of serine and glycine amino acid residues
二、日本血吸蟲單克隆杭獨特型抗體np30的單鏈狐（ scf ）的構建、表達及對balbic小鼠誘導保護性作用研究l 、日本血吸蟲單克隆杭獨特型抗體np0的單鏈抗體歸cfv ）的構建、表達通過pcr方法體外擴增並經測序驗證的重鏈、輕鏈可變區（ vh 、 vl ）基因先後重組入原核表達質粒ptha90相應的位點上，中間通過一連接肽（ gly在er ） 。In this thesis, first, reasons and factors for the stability of loose rock dams were summarized according to the field surveys and certain expert ' s experiences. then, researches on block stability problems were reviewed, and according to the relationship between grain size and incipient velocity, it is found that the block weight is in direct proportion to 6 - 9 power of incipient velocity, and 50 % increase of the velocity will result in about 40 times change of the block weight. after that, experiments were conducted in a flume, focusing on the relationship between incipient velocity and some main factors including block weight, water depth over the dam, cross - section size, block material and river bed material
本文首先根據散拋石壩損毀情況的現場調查資料，結合有關專家多年的整治經驗，總結出壩體的損毀原因及影響因素；對現有塊體穩定性的研究成果進行回顧和總結，並針對散拋石壩的直接損毀現象，結合山區河流的水流、地形特點，利用塊體粒徑與起動流速的關系，提出塊體穩定重量與起動流速高次方成正比的概念，流速50的增長可能導致塊體穩定重量接近40倍的變化；通過二維變坡水槽試驗，研究了壩體穩定的主要影響因素，包括塊體重量、壩頂水深、斷面尺寸、塊體材料（塊石和卵石兩種） 、護底等，結果表明對于山區河流，試圖僅僅通過增加壩體單個塊體重量或斷面尺寸來提高壩體穩定性效果甚微；最後，根據西部地區的自然、經濟、交通等條件，提出可以採用柔性混凝土鉸鏈體等一類整體性較好的護面層作為散拋石壩的防沖毀措施，以期取得良好的工程效果。This is important in attempting to use antibodies as anti - tumor reagents, for example
基因為日本血吸蟲單克隆抗獨特型抗體np30輕鏈和重鏈可變區基固。The library was rescued with phage m13k07 in order to display scfv on the surface of the phage and to form the recombinant phage antibody library. one of positive scfv clones, named pcsal, was selected with phage - elisa after panning and screening by bull sperm three times. scfv fragment, amplified from pcsa1, was ligated to pmd18 - t vector for sequencing analysis
取陽性重組噬菌體抗體克隆株pcsa1 ， pcr擴增其scfv基因，篩選重組子進行序列測定，發現其序列符合小鼠抗體基因的一般特徵，並且與幾株抗磷酸膽堿的抗體重鏈和輕鏈可變區序列的同源性達80以上；推測pcsa1scfv針對的抗原是磷酸膽堿類物質。In the present study, the express library of monoclonal anti - sp18 scfv ( single chain fragment variable ) gene is constructed and selected for further study of sp18 antigen on mammalian fertilization and embryogenesis. total rna were firstly isolated from these growing hybridoma cells which secretes monoclonal anti - sp18 antibodies. after obtained using rpas system, vh and vl genes were used to assemble scfv gene fragment with a linker primer
應用重組噬菌體抗體庫技術，從分泌小鼠抗牛精子sp18抗體的雜交瘤細胞系中分離總rna ，克隆抗體重鏈和輕鏈可變區基因，加入連接肽引物（ linkerprimer ）組裝成單鏈抗體scfv （ singlechainfragmentvariable ）基因並用rs引物進行擴增， sfi 、 not酶切，回收后與pcantab5e載體相連，轉化e . colitg1宿主菌，構建單鏈抗體文庫。Methods : a set of oligonucleotide primers were designed and used to amplify the vh and vl gene from anti - hbsag fab antibodies screened from phage antibody library. the products were cloned into puc19 vector and their sequences were analysed. the vh and vl gene fragments were tethered by a peptide linker and a leader sequence coding region, with the leader sequence added at 5 " terminus of each gene ( l - vh - linker - vl ) and designated as l - scfv
方法以從噬菌體抗體庫中篩選獲得的抗hbsag的fab抗體基因為模板，分別擴增出其輕、重鏈可變區（ v _ l 、 v _ h ）基因，通過重組pcr方法將輕、重鏈可變區基因用連接肽（ gly _ 4ser ） _ 3的編碼序列連接，並引入前導肽編碼序列，構建具有l - v _ h - linker - v _ l結構的單鏈抗體基因。This paper is based on the anthropologic research conducted in tangxiang, the small - sized pastoral community also the first township at the source of the changjiang river by means of ethnical records on the status of local herdsmen in high - altitude grassland ecological environment, the influences of local natural environment on local people and social organizations, the interactions between man and the ecological environment and the influences of national construction and economic development on local people and the environment etc. the paper testifies that the deterioration of local ecological environment is not merel y attributed to the restrictions arising from economic development and such natural factors as the increasingly warm global climate. the negligence of local people ' s initiatives and the impact on even the breakage of the cultural chain between man and the prairie ecological environment also play major roles
本論文通過對長江源頭第一鄉? ?唐鄉小型牧業社區的人類學考察，以民族志手法闡述本土牧民在高海拔草原生態環境中的位置、當地自然環境對人和社會組織的影響、人與自然生態環境的互動、以及國家建構和經濟發展對當地人與環境關系的影響等，論證當地生態環境惡化不僅是全球變暖等自然因素導致，也不局限於經濟發展等因素釀成，本土人群主體性被忽略、人與高原生態環境之間遊牧文化鏈被沖擊甚至被打破亦是不可忽視的重要原因。This paper is based on rs and gis, analyses the characteristics of luc of three periods in daqing city, discusses the traits of lucc of different periods, and discusses the driving forces from two aspects - nature factor and social factor, and forecasts the future land use pattern, points out the focus of land use continuable development. this research includes three significant problems, they are : the gaining of the lucc data in daqing area, the translation of land use pattern and its driving forces research, forecasts of the future land use pattern research. in the process of the study, we obtain the data that we need through manpower estimation and interpretation based on gis, then put the results into software envi, reclassify land use types using masking technology and decision tree
本次論文以黑龍江省大慶市為研究對象，基於遙感和gis平臺，提取了1979年、 1990年、 2001年區域土地利用/土地覆蓋數據，分析了研究區三個時期的土地利用/土地覆蓋特徵，利用單一土地利用動態度、綜合土地利用動態度、土地利用相對變化率等參數模型從土地資源數量、土地利用程度及土地利用區域差異等方面，探討了不同時期區域土地利用/覆蓋演化的特點，並從自然因素和人文因素兩個角度探討了區域lucc驅動力，最後利用馬爾科夫鏈模型對區域土地利用格局的發展趨勢進行了預測研究，提出了區域土地利用可持續發展的重點，為轉型時期的大慶市土地利用決策提供參考。分享友人