間隔片段 的英文怎麼說
中文拼音 [jiāngépiānduàn]
間隔片段
英文
stuffer fragment- 間 : 間Ⅰ名詞1 （中間） between; among 2 （一定的空間或時間里） with a definite time or space 3 （一間...
- 隔 : partitionseparate
- 片 : 片構詞成分。
- 段 : Ⅰ量詞（部分） section; segment; part; paragraph; passage Ⅱ名詞（姓氏） a surname
- 間隔 : interval; space; spacing; blanking; blank; region; distance; gap; intermission
- 片段 : part; passage; fragment; extract; segment; bit; episode; snatch; section
-
The c - phycocyanin ( cpc ) operon of blue - green alga ( or cyanobacteria ) arthrospira platensis fachb341 was cloned, sequenced and characterized by using chromoseme walking method. the sequence includes cpcb gene ( 519 bp ), cpca gene ( 489 bp ), cpch gene ( 357 bp ), and upstream sequence of cpcb ( 427 bp ) and upstream sequence of cpch genes ( 184 bp ), 111 bp of phycocyanin intergenetic spacer ( pc - igs ). upstream sequence of cpcb gene was ligated into promoter - probe vector pegfp - 1 and transformed into three systems : e. coli, synechocystis pcc 6803 and a. platensis fachb341 by supersonic and electrophoresis methods
根據genbank中報道的節旋藻藻藍蛋白基因序列設計引物，首先克隆了鈍頂節旋藻（ arthrospiraplatensisfachb341 ）藻藍蛋白操縱子中亞基基因、亞基基因部分序列及二者之間的間隔區序列（ pc - igs ）並進行序列測定，然後根據此測序結果設計引物，通過染色體步移法克隆得到藻藍蛋白操縱子長度為2086bp的基因片段，其中包括藻藍蛋白亞基基因（ cpcb ， 519bp ） ，亞基基因（ cpca ， 489bp ） ，連接蛋白h基因（ cpch ， 357bp ） ，亞基基因上游啟動子序列（ 427bp ）以及各基因之間的間隔區（ pc - igs ， 111bp ； cpch與cpca間隔區， 184bp ） 。It was interested that there was an extra six nucleosides insertion between 1647 - 1652nt ( according to the genomic sequence of la sota strain ), and the sequence were cccccc in f48e9 strain, and tcccac in zj1 strain. in order to test if insertion of this six nucleosides is related with the virulence of nd, two primers were designed to amplify the same fragment of another ten ndv strains. the result of sequence comparison of 16 strains showed that the six nucleoside was absent in lentogenic strain. this suggested that the six nucleosides insertion might have relationship with the ndv virulence. compared with all known sequence of ndv. there was a special sequence ( 5 ' tctctctcctctctcctcc3 " ) in the genomic cdna of ndv f48e9 strain
通過rt - pcr方法擴增獲得了另外10個背景清楚毒株的np - p基因間隔區片段，將這些序列與f48e9 、 lasota 、 clone30 、 b1 、 zj1和v4的相應序列進行了比較，結果在參比的16個ndv毒株中在該區段中除了有多個點突變外，個別毒株有堿基插入和缺失，所有以lasota株為代表的弱毒株均無6堿基的插入，而以f48e9株為首的強毒株均有此6堿基的插入，但有一個中等毒力的毒株dp沒有6堿基的插入，不過它的基因序列和lasota的幾乎相同，對于所克隆到的基因的代表性還有待確定。Application of the mf method normally involves construction of an intermediate column at every 4 meters. on a crossover section, which serves as a passage for train change directions, intermediate columns placed at 4 - meter intervals would block the route of a turning train. therefore, a solution must be found to the problem of location of intermediate columns on crossover sections
（四）轉轍段（ crossover ）處以多圓形潛盾工法施作之可能性，由於轉轍段系為提供電聯車換軌功能之處，兩軌道間不能有任何障礙物，而多圓形隧道環片間每隔四米即需中間柱，于設計上有何解決之道。
分享友人