限制氨基酸 的英文怎麼說
中文拼音 [xiànzhìānjīsuān]
限制氨基酸
英文
limiting amino acid- 限 : Ⅰ名詞(指定的范圍; 限度) limit; bounds Ⅱ動詞(指定范圍, 不許超過) set a limit; limit; restrict
- 制 : Ⅰ動詞1 (製造) make; manufacture 2 (擬訂; 規定) draw up; establish 3 (用強力約束; 限定; 管束...
- 氨 : 名詞[化學] (氮和氫的化合物) ammonia; hydrogen nitride
- 酸 : 酸構詞成分。
- 限制 : place [impose] restrictions on [to]; astrict; restrict; limit; confine; shut down on [upon]: 限制...
- 氨基酸 : amino acid; gamma-aminobutyric acid (gaba)
- 氨基 : amido group
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D - amino acids as unnatural chiral products are important intermediates in the synthesis of products, such as - lactam semisynthetic antibiotics, antiviral agents, artificial sweeteners, pesticide, peptide hormones, and pyrethroids, etc. though bioconversion of d - amino acids using microbial cells has been realized in industrialization for decades, some limited factors hinder the development of d - amino acid production, and ultimately result in shortage of the raw material in the related industrial fields
目前,用微生物菌體轉化生產d -型氨基酸的工藝路線已經產業化,但天然菌體轉化工藝存在一些限制因素,阻礙了d -型氨基酸的擴大生產,導致產品短缺。因此,利用基因工程菌生物轉化d -型氨基酸已成為世界氨基酸產業的新潮流。The ingredients restricted include wheat gluten, rice gluten, rice protein, rice protein concentrate, corn gluten, corn gluten meal, corn by - products, soy protein, soy gluten, mung - bean protein and amino acids
受限制的產品包括麥麩、大米蛋白粉、大米濃縮蛋白、玉米蛋白粉、大豆蛋白、綠豆蛋白和氨基酸等。Limited amino acid value
限制氨基酸值When both genes were co - expressed in e. coli, the activity of ppsa varied from 2. 1 - 9. 1 fold comparing to control, but the activity of tkta was relatively stable ( 3. 9 - 4. 5 fold ). whatever the two genes were expressed respectively or cooperatively, both could promote the production of dahp, the first intermediate of the common aromatic pathway, but co - expression was more effective on forming dahp and screened ppt - and ptp - as more effective. the results demonstrate that co - expression of ppsa and tkta can improve the production of dahp, and what ' s more, when multigenes co - expressed, the recombinant which has coordinated enzymes activity is optimum
莽草酸途徑的最優化和整體調控基因csra的敲除正是上述改變的分子基礎,同時也為三種芳香族氨基酸的基因工程菌的構建打下了基礎; 7 .在國內外首次實現了共同途徑限制性底物關鍵酶ppsa刁無『及arog與分支途徑關鍵酶基因phea的串聯高效表達,所構建的重組質粒ptga ,其ppsa 、 tkta 、 arog 、 cm和pd的酶活分別比對照提高了3 、 2 、 2 , 5 、 4 、 2 . 3倍,且其酶活比較協調一致; 8 .將ptga導入到篩選的基因敲除和基因替換菌株大腸桿菌31884 c甲b中,搖瓶發酵證實比以往所構建的基因工程菌株具有較高的phe產量和糖轉化率率,分別為0 . 448 %和22 . 4 % 。Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing
目的構建蛇毒鋸鱗蝰素( echistatin )的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素( echistatin )的氨基酸序列,結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性,設計了echistatin編碼基因,體外人工合成編碼基因dna片段,通過適當的限制性內切酶位點插入表達載體pbv220 ,分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆;進一步通過pcr技術構建echistatin的融合表達基因克隆。The insert dna fragments are 7kb and llkb, respectively. two subclones that were designated pgr3h1 and pgr7h1 and can increase glyphosate resistance of e. coli jm109 up to 150mm glyphosate were constructed by subcloning the 2. 4kb and 3. 2 kb hind ? / psti fragments of pgr3 and pgr7 into the corresponding sites of pgem - 3zf and pbluescript. sequence analysis of these two subclones revealed a completely identical 1323bp open reading frame that encodes an epsp synthase
以pgem - 3zf和pbluescript為載體,利用限制性內切酶hind和pst構建這兩個克隆的亞克隆,從中分別得到兩個草甘膦耐受亞克隆pgr3h1和pgr7h1 ,插入片段各為2 . 4kb和3 . 2kb ,對這兩個亞克隆進行序列分析,發現二者均含有一個核苷酸序列完全相同的完整的epsp合成酶基因? ? aroa ,其核苷酸序列長為1323bp ,推導的epsp合成酶由441個氨基酸組成,兩個亞克隆的草甘膦耐受濃度最大可達150mm 。All the subjects were genotyped by pcr - rflp ( polymerase chain reaction - restriction fragment length polymorphism ) at polymorphic sac i site inside the exon 7 of the ahsg gene. this polymorphism involves a nucleotide substitution of c to g at the middle nucleotide of the codon at amino acid position 238 resulting in the replacement of threonine ( acc ) with serine ( agc )
所有的樣本通過聚合酶鏈式反應?限制性片段長度多態性方法( pcr - rflp )對ahsg基因的第7個外顯子內的sac多態性位點進行基因分型,該多態性位點為238號氨基酸密碼子中間的堿基c到g的替換,使蘇氨酸( thr , acc )變為絲氨酸( ser , agc ) 。However, the yield of d - amino acids has been tempered by some limited factors from strain per se resulting in the shortages of that goods in the markets. therefore, scientists are trying to substitute engineered strains for natural strains in the process described above
目前,用微生物菌體轉化生產d -型氨基酸的工藝路線已經產業化,但天然菌體轉化工藝存在一些限制因素,阻礙了d -型氨基酸的擴大生產,導致產品短缺。分享友人