除蛋白酶的 的英文怎麼說
中文拼音 [chúdànbáide]
除蛋白酶的
英文
depepsinized- 除 : Ⅰ動詞1 (去掉) get rid of; eliminate; remove 2 [數學] (用一個數把另一個數分成若干等份) divide:...
- 蛋 : 名詞1. (鳥類或龜、蛇類所產的卵) egg 2. (像蛋形的東西) an egg-shaped thing 3. (辱罵之詞)
- 白 : Ⅰ形容詞1 (似雪的顏色) white 2 (清楚; 明白; 弄明白) clear 3 (空的; 沒加他物的) pure; clear; ...
- 酶 : 名詞[生物化學] (生物體的細胞產生的有機膠狀物質) enzyme; ferment
- 的 : 4次方是 The fourth power of 2 is direction
- 蛋白 : 1. (卵中透明的膠狀物質) egg white; albumen; gary2. [生物化學] (蛋白質) protein
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The effects of povidone - iodine and isothiozolone on the phaeocystis globosa ' s chlorophyl, protein and sod enzyme were studied, and the modality of the algae cell was observed by sem to understand the extinguishing mechanism of algaecide
摘要通過研究碘伏和異噻唑啉酮對球形棕囊藻葉綠素、蛋白質和超氧物歧化酶( sod )的影響,並使用掃描電子顯微鏡觀察棕囊藻的形態結構的破壞情況,初步探討了這兩種除藻劑單獨作用和復配使用時滅殺球形棕囊藻的機理。In the experiment, the full code sequence of bar gene was cloned by pcr from transgenic herbicide resistant bobwhite wheat and checked. it was expressed in e. coli and its protein was determined. after having been properly modified, the bar gene which correctly codes pat was cloned into binary vector pbi121 and then transferred into lba4404 by triparantal crossing, which is the prerequisite work for genetic transformation
本實驗從抗除草劑轉基因bobwhite小麥中,利用pcr克隆的方法擴增出bar基因全長,並在原核表達系統中表達,鑒定表達蛋白的活性,將能夠正確編碼ppt乙酰轉移酶的bar基因片段,經過適當的修飾構建入真核表達載體。Papaya tablet, papaya whistle wetting tablet papaya intestines dissolving capsule , papaya blood serum protein made by papain and other ingredients have the effects of detumescence, protection from inflammation, reliving pain, improving immunity, helping digestion, lustrating intestines parasites
用木瓜酶與其它成份配合製成的木瓜含片,木瓜潤喉片,木瓜腸溶膠囊、木瓜血清蛋白等藥品具有消腫、抗炎、止痛、提高免疫力、助消化、驅除腸道寄生蟲等功效。[ pharmaceutical industry ] papaya tablet, papaya whistle wetting tablet papaya intestines dissolving capsule , papaya blood serum protein made by papain and other ingredients have the effects of detumescence, protection from inflammation, reliving pain, improving immunity, helping digestion, lustrating intestines parasites
用木瓜酶與其它成份配合製成的木瓜含片,木瓜潤喉片,木瓜腸溶膠囊、木瓜血清蛋白等藥品具有消腫、抗炎、止痛、提高免疫力、助消化、驅除腸道寄生蟲等功效。They are involved both in the production of mature proteins, acting as processing enzymes, and in the degradation of damaged or non - functional proteins [ 1 " 8l in plant plastids, proteases function in protein degradation seem to act to adjust the stoichiometry of subunits in supramolecular complexes, as well as to regulate the stoichiometry between different supramolecular complexes in response to environmental changes and process the nuclear - encoded preproteins in the stromal
除了在相關蛋白質轉錄和翻譯方面的調控外,專一降解這些蛋白的蛋白酶活性以及轉錄翻譯量的上升也會加強對這些蛋白質的降解。雖然到目前為止對光系統中蛋白酶的了解尚且不如對光系統中各種多肽以及蛋白色素復合體的了解,但是對類囊體和葉綠體中蛋白酶的研究已經進展到相當深度了。Gc binds to mr with higher affinity than gr. this gc - receptor interaction is also controlled at the pre - receptor level by two important factors : corticosteroid binding globulin ( cbg ) and 11 b - hydroxysteroid dehydrogenases ( 11 - hsds )
糖皮質激素與受體結合的量除與血漿中糖皮質激素水平、皮質醇結合蛋白( cbg )濃度有關外,還受到細胞內受體前糖皮質激素代謝酶? 11 -羥基類固醇脫氫酶( 11 - hsd )的調節。The protein level of p21 increased lightly from 1 to3 h, then decreased at 6 - 9 h, reached maximum at 24 h after 2gy, it increased from 3 to 24 h exposed tology, and the protein level of p21 associated with cdk2 increased, which did not inhibit the cyclin e / cdk2 kinase activity, because of upregulation of cyclin e and deregulation of p27kipl after irradiation. in contrast to p21, p27kipl may be a key regulator of cyclin e / cdk2 activity in dna damage responses. hi the ubiquitin - proteasome pathway invovled in regulation of p27kiplexpression in response to ir the ubiquitin - proteasome pathway regulated selective and time - controlled elimination of p27 kipl
與p27ki 」 ,表達水平不同, pzi蛋白表達水平在zgy照射后略有增加, 6一gh略有下降,在照后24h達到最大; 10gy照后1一3h開始增加,持續到24h ,並且cycline / cdkz復合物中pzi蛋白結合增多,但cycline / cdkz激酶活性並未受到抑制,除了因ir誘導cydine蛋白表達水平增加外,另一重要的原因就是與pzi同一家族的p27kip ,蛋白表達水平下降,失去了對cycline / cdkz的抑制活性。The results of lauryl sodium sulfate - polyacrylamide gel electrophoreses ( sds - page ) of the aggregate precipitate and supernatant and the result of high - performance size - exclusion chromatography of the supernatant indicated that, by wrongly linked intermolecular disulfide bonds soluble bi - molecular and tri - molecular egg white lysozyme aggregate could be simultaneously formed except being renatured to native and active egg white lysozymes during the refolding procedure of denatured - reduced egg white lysozyme ; the aggregate precipitate could be further formed by the non - covalent bonds interaction between the soluble hi - molecular egg white lysozyme aggregates, and the soluble tri - molecular egg white lysozyme aggregate could still stay at the supernatant
沉澱和上清液的不連續十二烷基硫酸鈉聚丙烯酰胺凝膠電泳( sds - page )和高效凝膠排阻層析分析結果表明,還原脲變性蛋白溶菌酶在稀釋復性過程中除了能夠復性成天然態蛋白溶菌酶分子外,還會形成可溶的蛋白溶菌酶分子二聚體和三聚體,二聚體和三聚體主要是靠分子間二硫鍵的錯配連接而成的;可溶的蛋白溶菌酶分子二聚體之間通過非共價鍵相互作用而形成集聚體沉澱,而可溶的三聚體溶菌酶分子則仍處于復性液上清液中。There are active correlation between protein content and the growth stage of teleogryllus derelictus gorochov during its larvae and adult peiod. there are active correlation between the content of amino acids except cystine during larvae period. there are active correlation between the content of fat, total fatty acid, palmitoleic acid, oleic acid, ve2, vc and the activity of catalase and the total growth stage of it
蛋白質含量在若蟲和成蟲期與發育階段呈正相關,除胱氨酸外17種氨基酸含量在若蟲期與發育階段呈正相關,脂肪、脂肪酸、脂肪酸中軟脂酸和油酸、 v _ ( b2 ) 、 v _ c的含量及過氧化氫酶活力與發育階段呈正相關。Constructing cdna expressing library of erythrocytic plasmodium falciparum from hainan : the total rna was obtained by using. triplix kit. a modified oligo ( dt ) primer ( cds ffi pcr primer ) was used in the single - stranded ( ss ) dna synthesis reaction. the ss - dna was reversely transcripted from total rna. double - stranded ( ds ) cdna was amplified by long - distance ( ld ) pcrafter the digestion with proteinase k and sfi i, the cdna with no less than 200bp was collected and purified by glass - milk kitthe library was constructed after the ligation of cdna to tiplex2 phage particle packaged with the packaging extract system in vitro. a high titer and high recombinant ratio of cdna library was constructed
構建惡性瘧原蟲海南株紅內期cdna表達文庫提取紅內期惡性瘧原蟲海南株總rna ,直接以總rna為模板使用cdna文庫構建試劑盒,首先反轉錄合成ss一dna ,再擴增合成ds一dna ( cdna ) ,對擴增產物用蛋白酶k消化及左z丁i酶切,抽提蛋白、去除rna后,用玻璃奶試劑盒純化、回收20obp以上的片斷,經與載體連接再用蛋白包裝物包裝后形成未擴增文庫,最後擴增完成惡性瘧原蟲海南株紅內期cdna表達文庫的構建。Papain tablets, papain eating throat tablets, papain digestive capsule and papain serum etc made from papain compound can help ease swelling, alleviate inflammation, improve immunity, promote digestion and diminsh intestinal parasite
用木瓜酶與其它成份配合製成的木瓜含片、木瓜潤喉片,木瓜腸溶膠囊、木瓜血清蛋白等藥品具有消腫、抗炎、止痛、提高免疫力、助消化、驅除腸道寄生蟲等功效。The detection of coat proteins in host chloroplasts infected with tumv the intact chloroplasts were isolated, the proteins attached to the chloroplast surfaces digested, then the total proteins of chloroplasts extracted. the coat protein was detected in both chloroplasts of chinese cabbage and mustard infected with tumv through western blot
寄主葉綠體中tumv外殼蛋白的檢測通過提取青菜和芥菜葉片中的完整葉綠體,用胰蛋白酶消除其表面蛋白后,抽提葉綠體總蛋白,然後用westernblot檢測,證明ti1mh cp存在於感病寄主的葉綠體中。One 66kd band appeared except 44kd main band when go isozyme above was subjected to sds - page and ce - sds, indicating this go isozyme was similar to that from spinach leaves which contained 40kd and 66kd simultaneously. whether b - mercaptoethanol was added or not when go isozyme was subjected to in sds - page and ce - sds, 40kd main band and 66kd band still appeared, indicating two subunits were not linked by covalent disulfide. amino acid analysis shew that the ratios of basic to acidic amino acid of go isozyme and its 40kd acidic subunit were 0
菜心go同工酶的sds - page和sds -毛細管電泳( ce - sds )顯示,該酶除了含40kd主帶外,還有很淺的66kd帶,和之前我們提出的菠菜go同工酶含40kd酸性亞基和66kd堿性亞基相似; sos - page和ce - sds電泳中,無論加入-巰基乙醇與否, go同工酶都只有40kd主帶和66kd淺帶,表明菜心go同工酶中40kd酸性亞基和66kd堿性亞基不是以共價二硫鍵相連;用制備性sds - page法獲得菜心go同工酶的40kd亞基,並和菜心go同工酶一起測定其氨基酸組成,該go同工酶及40kd亞基的堿酸性氨基酸的比例分別為0 . 66和0 . 54 ,表明40kd亞基可能是個酸性蛋白,而66kd帶則是個堿性蛋白。Secondly, put acetone in the abstracting liquor, put it aside for one hour and slowly centrifuge. thirdly the deposit is fully dissolved and heated for 15 minutes in 50 c water, and get crude enzyme solution through centrifuge
分離純化將蘆薈的葉子洗凈、擦乾,加入tris - hcl緩沖液進行組織搗碎提取sod ,高速離心;將提取液用丙酮使sod沉澱、靜止,低速離心;將沉澱用蒸餾水充分溶解, 50水浴中加熱15min ,離心除去部分雜蛋白,獲得粗酶液。Phopholipase c - 1 ( plc - 1 ) is widely known to play an important role in regulating cell proliferation and differentiation, development of the organisms, cell transformation and oxidative stress. till now, the mechanism how phopholipase c - 1 acts can not be thoroughly illustrated, nor has the interaction between plc - 1 pathway and other signal pathways been systematically reported. this research chose 2 - de + ms as the basic method from all kinds of proteomics strategies and compared the total protein expression map of mef genetically deficient in plc - 1 ( plc - 1 - / - ) to that of wild type mef ( plc - l + / + ) aimed to find some protein spots differentially expressed, thus we can discuss the impact of knockout of plc - 1 on signal transduction initiated by growth factors such as egf comprehensively. in this way, we can study the biological function of plc - 1 and mechanism of plc - 1 pathway indirectly, which will contribute a lot to further analysis
鑒于plc - 1發揮上述作用的機制尚未完全闡明, plc - 1通路與其他信號通路間的交聯和代償尚無系統報道,又因為以往的研究方法不夠全面,本研究以野生型小鼠胚胎成纖維細胞( plc - 1 ~ ( + / + ) )和缺失磷脂酶c - 1的小鼠胚胎成纖維細胞( plc - 1 ~ ( - / - ) )為研究模型,在眾多蛋白組學策略中選擇了雙向電泳+質譜( 2 - de + ms )作為研究手段,通過對比表皮生長因子( egf )刺激24小時後上述兩種細胞的總蛋白質表達差異,全面地探討敲除plc - 1對生長因子誘導的信號傳遞的影響,從而間接研究plc - 1生物學作用、信號傳遞機制及其代償情況,為后續的深入研究打下基礎。This expression vector plbcas - hsa - lgl has the following advantages : i ) the 1. 7kb promoter is able to drive cell - specific and hormone - dependent expression ; ii ) the inclusion of intron - 1 can increase expression level of fusion genes ; iii ) the 5 ' utr of bovine p - casein mrna may have a positive role in both transcriptional and post - transcriptional regulation ; iv ) the gfp gene make the selection of positive clone among embryos possible ; v ) the gfp gene can be easily excised via cre - mediated recombination between the two loxp sites after the expression vector has been integrated into chromosome ; vi ) the two incompatible lox sites, loxp and lox2272, would facilitate cre - recombinase mediated cassette exchange ( rmce ), which in theory will leading to develop a technology of site - specific gene expression in animal mammary glands
該載體的特點是:具有可以調控外源基因在乳腺中特異表達的牛-酪蛋白基因5 `端側翼區和包括第一外顯子及內含子在內的5 `端調控區;將人血清白蛋白cdna準確地置於牛-酪蛋白基因第二外顯子中的翻譯起始密碼子atg之後,而且沒有增加額外的序列和使人血清白蛋白cdna移碼;引入標記基因gfp ,便於在胚胎期鑒定陽性胚胎,減少受體;引入cre lox重組系統: ( ? )標記基因gfp的兩端的兩個loxp位點可以在表達載體整合到基因組后,刪除標記基因; ( ? )餘下的一個loxp位點可以和前面的lox2272位點組成cre重組酶介導的盒式交換系統。In this study, a new approach of plant virus - resistance was expored by making up the cdna of ppiv. nib gene was amplified from plasmid pysr, which is plant expression vector containing potyvirus y nib gene. the nib gene was fused with the fragment encoding the mature part of papaya proteinase iv
將番木瓜蛋白酶的cdna序列以全長、去除信號肽部分和成熟酶部分分別連接到細菌表達載體中,表達結果表明,只有含去除信號肽部分cdna的載體才能檢測到相應的表達的蛋白條帶。A defective protein, which binds polyubiquitin chain ( so - called " degrading label " ) through its - amino group of lys residue, is degraded in the proteasome
對于需要清除的蛋白質,通過其賴氨酸殘基側鏈-氨基連接多聚泛素鏈(降解標簽) ,繼而在蛋白酶體中被降解。Exposure of cells to the fungal inhibitor wortmannin eliminated focus formation by all repair factors examined, suggesting a role for the phosphoinositide ( pi ) - 3 family of protein kinases in mediating this response
抗真菌藥物渥曼青霉素作用於細胞,能通過檢測到的修復因子消除形成的病灶,這說明了蛋白激酶中磷酸肌醇家族在這一反映中所起的作用The lhcii, which lies outside, was digested partly in both naci - washed psil particle binding the 33 kd protein and caci2 - washed psil particle lacking the 33 kd protein, independent of binding of 33 kd protein. however, caci2 - washed psil particle was more sensitive to tryptic attack than naci - washed psil particle, especially, cp43 decreased more significantly in caci2 - washed psil particle than in naci - washed psil particle. oxygen - evolving psil core complex was sensitive to trypsin digesting, cp43, d2, d1 and 33 kd protein were digested even under slight trypsin treatment and the fragment were verified by western blotting
結果顯示:在nacl鹽洗ps顆粒和cacl _ 2處理ps顆粒中, lhc容易被酶解; cacl _ 2處理ps顆粒比nacl鹽洗ps顆粒對胰蛋白酶作用敏感;與nacl鹽洗ps顆粒相比,在除去33kd蛋白后, cacl _ 2處理ps顆粒的cp43更容易被酶解;放氧核心復合物對胰蛋白酶更敏感,在低濃度的胰蛋白酶作用下, cp47不被水解,而cp43 、 d _ 2 、 d _ 1和33kd蛋白被部分水解, western - blotting可以檢測到它們的水解片段。分享友人