隆亞甲 的英文怎麼說
中文拼音 [lōngyǎjiǎ]
隆亞甲
英文
long akah-
After our beautiful and gracious supreme master ching hai lectured in kuala lumpur, malaysia, on april 30, 2000, local initiates were filled with happiness and overflowing with love. our enthusiasm to share master s teachings could not be contained, and so with a quan yin messenger, malaysian initiates presented six video seminars in the major cities of malaysia, namely, perak, kadah, ipoh, butterworth, malacca, and penang
自從我們美麗優雅的清海師父在2000年4月30日蒞臨馬來西亞吉隆坡講經之後，同修們法喜充滿愛力洋溢，熱切地想要分享師父教理給更多人，於是與觀音使者一起在馬來西亞各主要城市舉辦了六場錄影帶講座，這些城市包括比叻卡達怡保巴特渥斯麻六甲與檳城。One of the most famous battles of the north african campaign pitted rommel s panzer korps against the australian army
利比亞之戰隆美爾的裝甲部隊力撼企圖切斷其補給線的澳軍，戰況慘烈。In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals
首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
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