隆恰克 的英文怎麼說
中文拼音 [lōngqiàkè]
隆恰克
英文
lancsak-
The 3. 4kb ecori dna fragment of the pgxn201 and the mutant ecori dna fragments of the three mutant plasmids were subcloned into the ecori sites of the clone vectors ml3 and the pgem3z - f ( + ) respectively. through dna sequencing and dna sequence analysis by comparing with the bradyrhizobium japonicum dna sequences reported, we found that there are four intact open reading frames in the 3. 4kb fragment and it was highly homologous to the bradyrhizobium japonicum dna sequence reported, and the tn5gusa5 containing in the pgxn217 was inserted in one of the four open reading frames. this open reading frame located on about 5. 5kb upstream of the nfec gene, which includes 207bp nucleotides, coding 68 putative amino acids and this is completely identical to that of the bradyrhizobium japonicum dna sequence reported
本研究首先利用轉座子tn5gusa5對重組質粒pgxn201進行誘變，獲得3 . 4kbecori片段插入了tn5gusa5的突變質粒pgxn215 、 pgxn216 、 pgxn217 。對這些突變質粒進行亞克隆及測序分析並與基因庫中已報道的慢生型大豆根瘤菌的dna序列作比較，發現突變質粒pgxn217中tn5gusa5恰好插入在一個未知功能的開放閱讀框架內。將pgxn201的3 . 4kbecori片段與m13作連接，獲得亞克隆pgxn201c ，對pgxn201c進行測序分析，發現與基因庫中已報道的慢生型大豆根瘤菌的dna序列有95的同源性。
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