隨機基因突變 的英文怎麼說
中文拼音 [suíjījīyīntūbiàn]
隨機基因突變
英文
random genetic mutation- 隨 : Ⅰ動詞1 (跟; 跟隨) follow 2 (順從) comply with; adapt to 3 (任憑; 由著) let (sb do as he li...
- 機 : machineengine
- 因 : Ⅰ動詞[書面語] (沿襲) follow; carry on Ⅱ介詞1 [書面語] (憑借; 根據) on the basis of; in accord...
- 突 : Ⅰ動詞1 (猛沖) dash forward; shoot out 2 (高於周圍) protrude; bulgeⅡ副詞(突然) abruptly; sud...
- 隨機 : random stochasticrandom
- 突變 : 1 (突然急劇的變化) sudden change; change suddenly; transilience; accident; saltation; revulsion...
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Dominant negative alleles of gpil7p were produced by random pcr mutagenesis of three conserved fragments of gpi17p : 1 ) having generated the plasmid library which contains 100000 plasmids harboring gpi17 mutants then screened from 8000 clones, we got 25 strong and 3 very strong dominant negative alleles
對gpi17p可能的三個保守區進行了pcr隨機誘變,並最終獲得顯性負性等位基因: 1 )組建了gpi17p突變體質粒庫,其所含質粒數約為100000 。Neutral mutation - - evolution at the molecular level is primarily determined by mutational input and random genetic drift, rather than by natural selection
天然突變- -演化過程中在分子的層次上主要是經由隨機的基因流而決定,並非藉由天擇所影響。Red tide occurs randomly, with short duration and large incidence. airborne hyperspectral remote sensing technique can play an important role in red tide ' s monitoring. and the precondition is to acquire in situ spectral data of red tide, to grasp red tide ' s spectral characteristics by data processing and analysis and to set up spectral library of different dominant species of red tide
赤潮是海水中的浮游生物在一定條件下過度繁殖或聚集致使海水變色的一種生態異常現象,因其具有隨機性、突發性、持續時間短、影響范圍大等特點,客觀上要求利用航空高光譜遙感技術對其進行監測;但首要的前提與基礎性工作是大量獲取赤潮水體的現場光譜數據,通過處理與分析,掌握其光譜特性,不斷充實赤潮優勢種類的光譜數據庫。Two pta - mutants have been selected by using suicide substrate after the mini - tn5 transposon insertion mutagenesis of klebsiella pneumoniae m5al. when used in microaerobic fermentation, the amount of acetate produced by the mutants reduced to less than 50 % of the parent strain, and the yields improved whereas the 1, 3 - propanediol titers and productivities decreased
以klebsiellapneumoniaem5al為出發菌株,用mini - tn5隨機轉座誘變結合自殺性底物篩選的方法得到了兩株產乙酸途徑pta基因缺失突變株xl - 6和xl - 11 ,應用到微氧法發酵中,突變株的乙酸產量為親株的50以下,甘油轉化率有所提高,但1 , 3 -丙二醇濃度和生產強度有所下降。It clearly allows us to engineer enzymes with novel functions and features by strategies, such as error - prone pcr, dna shuffling and stagger extension process ( step ), when very little is known about spatial configuration of proteins. therefore millions of years in nature can in principle be accomplished in the test within several years. according to the basic idea of directed evolution, we modified the a - aldc gene by error - prone pcr
它不需要事先了解酶的空間結構和催化機制,而通過在體外模擬自然進化機制(隨機突變,重組和自然選擇) ,利用易錯pcr , dna改組,交錯延伸等方法,改造酶基因,定向選擇(或篩選)有價值的非天然酶,短期內在試管中完成在自然界需要幾百萬年的進化過程。Objective to establish a complex pcr method and to investigate the genetic polymorphism and population difference of three loci on y - chromosome, so as to provide a database for forensic medicine casework. methods edta - blood specimens were collected from 163 unrelated males in han population in taiyuan. different tissues of one corpse were analyzed including blood, muscle, liver and kidney
方法( 1 )樣本採集:隨機抽取163名太原地區漢族無親緣關系的男性個體的靜脈血, edta抗凝,進行dys390 、 dys391和dys393基因座等位基因及單倍型頻率分佈調查;採集同一屍體血液、肌肉、肝臟、腎組織進行同一性檢測;採集20例兩代家系血進行突變觀察;取20例女性個體血進行男性特異性檢驗。The mutant pel - d92l was expressed in pichia pastoris gs115, sds - page detection showed that the expression product pel - d92l - gs is different from pel - gs, and its " yield decreased dramatically, the themostability of pel - d92l - gs is also different from the pel - gs, but their optimum temperatures are same. 3. directed evolution of pel through random mutagenesis mutagenesis pcr carried out in error - prone conditions was used on the vector psk - pel, using the oligos " beginning " and " end ", homologous to the 5 ' and to the 3 " ends of the gene of pel respectively
三、 pel基因的隨機誘變用易錯pcr方法對pel基因進行隨機誘變, pcr產物與ppic3 . 5k連接,轉化大腸桿菌,獲得的混合質粒電轉化畢赤酵母gs115 , omm平板篩選適于低溫或對熱穩定的重組子,篩選獲得一株最活反應溫度、熱穩定性、發酵酶活均有提高的突變體pel - ep5 - gs ,其最活反應溫度為45 ,比野生型高出5 ; 40處理30min殘留活性為56 ,大大高於野生型的6 ;初始ph7 . 3528條件下培養72h ,發酵上清酶活為325u / ml 。Eventually another random mutation to a cancer gene knocks down another obstacle, initiating another burst of growth
直到有另一個癌癥基因產生隨機突變,去除了另一個阻礙,而引發另一波的生長。It evolves in two ways : gradually through random mutation, and more rapidly as different strains of influenza swap genes inside a single animal or person [ see box on opposite page ]
病毒可經由兩條演化途徑得到在人類間散播的能力:隨機突變而逐漸適應,更快的是在同一隻動物或人體內與其他流感病毒交換基因(參見右頁全球流感病毒株的衍生途徑) 。Then, to get more knowledge about the rpob gene mutations and provide some scientific information to study the molecule mechanism of resistance to rifampin in mycobacterium tuberculosis, the rpob gene sequence of mycobacterium tuberculosis clinical isolates from shannxi was analysed by direct sequencing of pcr products
隨后通過直接測序法對陜西區域內結核分枝桿菌臨床分離株rpob基因pcr擴增產物進行序列分析,了解該區域rpob基因突變情況,為研究結核分枝桿菌耐利福平的分子機制提供參考。It is used to probe the relationship between the structure and activity of proteins ; to define the roles of particular proteins and protein assembles in the cell ; to determine the logic and order of molecular events during differentiation and morphogenesis ; to improve the activity of enzyme, antibiotics and so on
引導蛋白質功能進化常用的方法是模擬和加速蛋白質基因自然重組的進程,即在蛋白質的基因中引進隨機突變。因此,蛋白質基因體外隨機突變的方法影響著引導蛋白質功能進化的效果。分享友人