集聚蛋白 的英文怎麼說
中文拼音 [jíjùdànbái]
集聚蛋白
英文
agrin- 集 : gatherassemblecollect
- 聚 : 動詞(聚集; 聚積) assemble; gather; get together
- 蛋 : 名詞1. (鳥類或龜、蛇類所產的卵) egg 2. (像蛋形的東西) an egg-shaped thing 3. (辱罵之詞)
- 白 : Ⅰ形容詞1 (似雪的顏色) white 2 (清楚; 明白; 弄明白) clear 3 (空的; 沒加他物的) pure; clear; ...
- 集聚 : 1. (集合; 聚合) gather; collect; assemble 2. [訊] (掃描行的合併) grouping3. (集中; 濃縮) concentration
- 蛋白 : 1. (卵中透明的膠狀物質) egg white; albumen; gary2. [生物化學] (蛋白質) protein
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Strain bl21, and gene expression was induced by iptg. the target proteins were directed into the periplasmic space by the staphylococcal protein a signal sequence preceding the rgd - hirudin gene. using ion exchange chromatography and gel filtration chromatography, the chimera proteins were purified, and both of them showed a single band in tricine - sds - page. the results of activity analysis suggested that these two chimera proteins not only have antithrombin activities, but gain platelet aggregation inhibitory activities as well
通過離子交換層析和凝膠過濾層分別對兩種嵌合體蛋白進行純化,純化產物在tricine - sds - page中都顯示為單一條帶。活性分析結果表明兩種嵌合體蛋白在保留水蛭素抗凝血酶活力的同時,還呈現抗血小板聚集活性。The masp ( mannose - binding lectin - associated serine protease ) gene has been cloned by the method of degenerative pcr and the fragment of the pcr product is 630 bps in length
本文還利用pcr方法從青島文昌魚基因組dna中克隆masp (甘露聚糖結合凝集素相關絲氨酸蛋白酶)基因片段。To dress the question if other virulence gene were present in this kind of strains, 152 of 436 irp2 - hybridized strains were re - confirmed and selected for this study. the virulence genes or putative virulence genes detected by pcr or hybridization include heat stable toxin ( st ) & heat labile toxin ( lt ) for enterotoxigenic e. coli ( etec ), invasive plasmid antigen b ( ipab ) for enteroinvasive e. coli ( eiec ), epec adherence factor ( eaf ), epec secretion protein c ( espc ) for enteropathogenic e. coli ( epec ), hemolysin ( hlya ) and shiga toxins ( sltl and slt2 ) for enterohaemorrhagic e. coli ( ehec ) and eaggec probe for entero - aggregative e. coli ( eaggec ). the prra and yc73 genes of pathogenicity associated island ( pai ) of urepathogenic e. coli ( upec ) and " o " island 28 ( rtx 615 ) gene was also detected, the later was a newly discovered putative pathogenicity island in e. coli o157 : h7
為探討攜帶小腸結腸炎耶爾森氏菌的hpi毒力島的大腸桿菌是否具有其他已知的毒力基因,選取82株由原位雜交和pcr方法初篩irp2陽性的大腸桿菌菌株,進行在致瀉性大腸桿菌的25個毒力基因的檢測,包括腸產毒性大腸桿菌的熱穩定毒素st和熱不穩定毒素lt ,腸侵襲性大腸桿菌的侵襲蛋白b基因ipab ,腸致病性大腸桿菌的eaf 、 espc基因,腸出血性大腸桿菌的溶血素hly 、志賀毒素1 ( slt1 ) 、志賀毒素2 ( slt2 )基因,腸集聚性大腸桿菌的eaggec探針,以及在泌尿道致病性大腸桿菌和o157 : h7大腸桿菌中新發現的毒力島基因。There are some nutritional factors and antinutritional factors in soy, nutritional factors include soy phospholipid and soy isoflavone, antinutritional factors include trypsin inhibitors, antigenic protein, lectins and phytic acid, soy oligosaccharides and soy saponins have nutritional and antinutritional effects
摘要大豆中含有抗營養因子和營養因子,營養因子包括大豆磷脂、大豆異黃酮等,抗營養因子包括蛋白酶抑制因子、抗原蛋白、凝集素、植酸等,大豆低聚糖和皂甙具有營養和抗營養雙重作用。A page gel, stained by the petiodic acid - schiff ' s method to reveal glycoproteins, further displayed that the bindng - protein was a glycoprotein belonging to lectin, which contained 17. 4 % ( w / w ) neutral carbohydrate content of the glycoprotein detected by the phenol / h2so4 method. peptide mapping was comparable to the reported protein in protein bank. the database homology search ( ncbi blast ) indicated that the binding - protein shared 70 - 80 % homogeneity to l - aspartate oxidase, aspartyl / glutamyl - trna ( asn / gln ) amidotransferase subunit b, glutamyl - trna reductase, histidyl - trna synthetase
連續梯度聚丙烯耽胺凝膠電泳、 sds一聚丙烯酞胺凝膠電泳和等電聚焦的結果表明該蛋白分子量為1 「 . skda ,由二個相同的亞基組成,亞基分子量為」 . ikda ,等電點為8 . 25 .糖蛋白染色結合考馬斯亮藍染色的結果證實此結合蛋白是個糖蛋白,其中糖含量為17 . 44 % ,蛋白含量為82 . 56 % .凝集反應確定該糖蛋白也是一個凝集素The results of lauryl sodium sulfate - polyacrylamide gel electrophoreses ( sds - page ) of the aggregate precipitate and supernatant and the result of high - performance size - exclusion chromatography of the supernatant indicated that, by wrongly linked intermolecular disulfide bonds soluble bi - molecular and tri - molecular egg white lysozyme aggregate could be simultaneously formed except being renatured to native and active egg white lysozymes during the refolding procedure of denatured - reduced egg white lysozyme ; the aggregate precipitate could be further formed by the non - covalent bonds interaction between the soluble hi - molecular egg white lysozyme aggregates, and the soluble tri - molecular egg white lysozyme aggregate could still stay at the supernatant
沉澱和上清液的不連續十二烷基硫酸鈉聚丙烯酰胺凝膠電泳( sds - page )和高效凝膠排阻層析分析結果表明,還原脲變性蛋白溶菌酶在稀釋復性過程中除了能夠復性成天然態蛋白溶菌酶分子外,還會形成可溶的蛋白溶菌酶分子二聚體和三聚體,二聚體和三聚體主要是靠分子間二硫鍵的錯配連接而成的;可溶的蛋白溶菌酶分子二聚體之間通過非共價鍵相互作用而形成集聚體沉澱,而可溶的三聚體溶菌酶分子則仍處于復性液上清液中。Systematic cluster analysis was carried out on hu sheep in china in comparison with the same data of 9 asia sheep populations and 5 european sheep ( breeds in japan ) populations. 15 populations can be clustered in terms of gene frequency of 10 loci and 33 allele in blood enzyme and other protein variations
摘要以中國湖羊為研究對象,搜集國內外9個亞洲綿羊群體和5個在日本的歐洲綿羊群體的相同資料作為對照,根據控制血液酶和其他蛋白質變異的10個基因座位共計33個等位基因的頻率,進行系統聚類分析。Thin sections of host leaf cells infected by bbwv - 2 isolate b935, which were gold - labeled by antibodies of bbwv - 2 coat protein ( cp ) and vp37, respectively, were prepared to elucidate the locations of vp37 in cell and possible function of vp37 and cp in cell to cell movement. observation in electron microscope showed that virus particles were presented not only in cytoplasma but also in chloroplast, while vp37 was existed only in cytoplasma and associated with tubular structure through the cell wall
為研究vp37在寄主細胞中的作用機制及其在細胞中的分佈,通過膠體金間接標記6his - vp37兔抗血清,同時還標記了病毒的外殼蛋白單克隆抗體,對bbwv - 2分離物b935感染的病葉超薄切片的電子顯微鏡觀察發現:病毒粒子除了聚集在胞質中,還存在於寄主的葉綠體內; vp37蛋白能在細胞壁上形成管狀結構,在胞質中亦有分佈。Quantitatively describing the principle of protein - protein interactions and recognition in molecular level is the key point for understanding the relationship between structure and function of protein complexes and designing protein complexes, hi these interactions among proteins, electrostatic and hydrophobic interactions are the two most important ones, which let protein molecules bond systematically
在分子水平上定量地闡明蛋白質分子之間的親合力和相互識別的機制,是正確地認識蛋白質聚集體結構和功能之間的關系併合理地設計出有用的蛋白質分子聚集體的關鍵。蛋白質分子間相互作用,其中最主要的是靜電和疏水相互作用,使蛋白質分子能有序地結合在一起。A novel aqueous two - phase system can be formed by the mixtures of a polymer and cationicanionic surfactants. such a system can be used as a partitioning system of proteins. in this work, we investigated the formation, phase behavior and protein partitioning in aqueous two - phase systems formed by dodecyltriethylammonium bromide / sodium dodecylsulfate / peg and dodecyltriethylammonium bromide / sodium dodecylsulfate / dextran. the ligands with affinity were attached to the polymers and the affinity partitioning of proteins was investigated. it was shown that the surfactants and polymers are enriched in different phases of aqueous two - phase systems. phase separation are promoted by increasing temperature and adding inorganic salts. different proteins are partitioned in different phases. the selectivity of protein partitioning is increased by adding ligands with affinity
報道了由正負離子表面活性劑與高聚物混合溶液形成的一種可用於蛋白質的分離及分析的新型雙水相萃取體系.研究了正負離子表面活性劑(溴化十二烷基三乙銨/十二烷基硫酸鈉)分別與葡聚糖和聚乙二醇混合雙水相體系的形成規律、相行為及牛血清蛋白和溶菌酶在雙水相體系中的分配.通過在高聚物分子中接上親和配基,研究蛋白質在雙水相體系中的親和分配.結果表明,在該體系中,表面活性劑與高聚物分別富集於不同相中.升高溫度及加入無機鹽均可促進雙水相體系的形成,不同蛋白質可分配于不同的相中.親和配基的引入極大地增強了蛋白質分配的選擇性The aggregate of egg white lysozyme molecules formed during the refolding procedure of denatured - reduced egg white lysozymes was analyzed by protein electrophoreses and high - performance size - exclusion chromatography
本文利用蛋白電泳和高效凝膠排阻層析法分析了還原脈變性蛋白溶菌酶稀釋復性過程中的集聚體。The concentration dependence of bsa apparent diffusion coefficients was interpreted in the context of a two - body potential of mean force that includes repulsive hard - sphere and coulombic interactions and attractive dispersion : with increasing ionic strength, debye screening decreased, and protein interaction changed from repulsion to attraction, and protein aggregations was onset
利用平均勢場理論的兩體硬球相互作用模型解釋了蛋白分子的相互作用變化規律:隨著離子強度的增加,離子氛厚度減小,蛋摘要白分子間的相互作用由排斥變為吸引,蛋白開始聚集。Gly - pro - arg - pro acetate salt ( gprp ) was applied to prevent platelets aggregation and fibrin formation, stabilize platelets and minimize the artifical platelets activation. this is also the key to conquer difficulty of flow cytometric quantitive analysis when platelet, ca2 + and plasma coexist. this flow cytometric method is specially suitable for the multi - param
該方法中glypro argpro乙酸鹽( gp肝)的選擇應用,既防止了血小板聚集和纖維蛋白的形成,同時可穩定血小板,減少制備過程中人為激活,克服了在血小板、 c嚇血漿共存條件下fcm定量分析血小板的困難,尤其是為深低溫處理血小板包括ps在內的多參數分析提供了良好的方法基礎。There are at least three kinds of ep in petal : thiol - proteinase, metallo - proteinase, serine proteinase. they gather at the site between 43 kd and 66. 2 kd ( about 52 kd ) on the substrate gel electrophoresis. the optimum ph of thiol - proteinase, metallo - proteinase is ph 6, which are looked as acid ep, the serine proteinase has two forms, one is acid ep ( ph 6 ), and the other is alkali ( ph 10 )
花瓣中都至少有3種內肽酶:巰基蛋白酶、金屬蛋白酶、絲氨酸蛋白酶,三者聚集在底物膠電泳膠條的43kd 66 . 2kd之間的位置,約為52kd ;前兩種最適ph為6 ,是酸性內肽酶,后一種有酸( ph6 ) 、堿( ph10 )兩種存在形式,既是酸性內肽酶又是堿性內肽酶。In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ), and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee. the plasmid pfb 1 - cee was transformed into dh1 obac competent e. coli cells. high molecular weight dna was prepared from the overnight cultures from the selected e. coli colonies, which was recombinant baculovirus shuttle vector containing hcv structural gene, named bac - cee
本實驗用pcr擴增hcv結構區基因,克隆到桿狀病毒表達載體pfastbacl中,構建成重組轉座載體pfb1 - cee ,轉化dh10bac大腸桿菌感受態細胞,篩選陽性菌落,抽提大分子質粒dna ,獲得含hcv結構區基因的重組桿狀病毒穿梭載體bac - cee ,脂質體介導轉染sf9昆蟲細胞,出現細胞病變后,收集含有重組桿狀病毒顆粒的培養上消,重新感染sf9細胞,收集sf9細胞,進行12 . 5 sds -聚丙烯酰胺凝膠電泳,可見表達的蛋白條帶。One set of proposals theorizes that small platforms made up of a few protein molecules each may be clustered in cell membranes and capable of moving around the cell surface together, most likely with help from the cytoskeleton
其中一套理論認為,少許蛋白分子組成了小平臺,每一個小平臺都可能聚集在細胞膜上,而它們也可以一起在細胞表面移動(極可能是透過細胞骨架的協助) 。Some new trends in the study of metalloproteins and metalloenzymes have been described from aspects of metal ions - related diseases ( especially the neurodegenerative diseases ), the roles of metal ions in folding, aggregation and assembly of proteins, the design and reconstruction of metallochaperons and metalloproteins, interactions between metalloproteins and dna
本文敘述了金屬蛋白和金屬酶研究中近年來幾個值得注意的發展動向,即與金屬離子相關的疾病(特別是神經退行性疾病) 、金屬離子在蛋白質的折疊、聚集和裝配中的作用、金屬伴侶分子、金屬蛋白的設計和構建、金屬蛋白與dna相互作用。Method to collect respectively 180 unrelated males " venous blood 500ul, who lived in shanxi province, 120 unrelated mongolians " venous blood 500ul, who lived in the inner mongolia autonomous region, and the blood is anticoagulant with edta, then to extract dna by using the method of phenol - chloroform after ingested by proteinase k at 56 and amplify the dys413 site by using pcr
方法採集180例山西漢族和120例內蒙古蒙古族男性無關個體靜脈血各500ul , edta抗凝,用tkml液反復洗滌至無色,加入2蛋白酶k緩沖液180ul ,蛋白酶k ( 20mg ml ) 20ul ,在56消化至液體清亮為止,用酚-氯仿法抽提dna , pcr擴增dys413位點, 6非變性聚丙烯酰胺凝膠電泳, 1硝酸銀染色分型。Researchers hope to slow or een preent the deelopment of alzheimer ' s by reducing or preenting the aggregation of amyloid beta
研究者試圖通過減少或阻止澱粉狀蛋白的聚集來延緩甚或阻止阿耳茨海默氏病的發展。At least two other mechanisms could play a role in organizing proteins at the synapse, but the extent of their influence on immune cell communication is controversial
起碼還有另外兩種機制,也在突觸蛋白的組織聚集上扮演一角,但它們對免疫細胞溝通有多少影響力,卻眾說紛紜。分享友人