集落因子抗原 的英文怎麼說

中文拼音 [jílàyīnzikàngyuán]
集落因子抗原 英文
colonization factor antigen
  • 集 : gatherassemblecollect
  • 落 : 落名詞[方言] （北方對蓮花落的俗稱） a kind of folk song
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  • 子 : 子Ⅰ名詞1 （兒子） son 2 （人的通稱） person 3 （古代特指有學問的男人） ancient title of respect f...
  • 抗 : Ⅰ動詞1 （抵抗; 抵擋） resist; combat; fight 2 （拒絕; 抗拒） refuse; defy 3 （對等） contend with...
  • 原 : Ⅰ形容詞1 （最初的; 原來的） primary; original; former 2 （沒有加工的） unprocessed; raw Ⅱ動詞（原...
  1. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  2. Cd 14 * monocytes or bone marrow derived hematopoietic progenitor cells cultured with granulocyte - macrophage colony - stimulating factor ( gm - csf ) and il - 4 differentiate into efficient apc with morphology and cell surface molecule expression typical of immature dc. it was demonstrated that a variety of factors ( such as lps, tnf - a, il - 1, monocyte - conditioned medium, and cd40 receptor cross - linking ) induce the maturation of immature dc to mature dc that are much more potent in activating t cells by increasing the expression of costimulatory molecules and cytokines, promoting the migration of dc to draining lymph nodes, and down - regulating the capacity of dc to capture and process antigen

    在體外，外周血cd14 ~ +單核細胞和cd34 ~ +骨髓造血前體細胞在粒細胞-巨噬細胞集落刺激因子（ granulocyte - macrophagecolony - stimulatingfactor ， gm - csf ）和il - 4的共同作用下可分化為未成熟dc ，未成熟dc具有強大的攝取和處理抗原的能力，但其抗原提呈能力較弱；未成熟dc在炎性細胞因浙江大竿俗士學位論文潛廷平子汐ilq ， yfax病原成分汐lpsx單核細胞條件培養液或cd40l等作用下可分化為成熟dc 。
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