雜交酶 的英文怎麼說

中文拼音 [zájiāo]
雜交酶 英文
hybrid enzyme
  • 雜 : Ⅰ形容詞（多種多樣的; 混雜的） miscellaneous; varied; sundry; mixed Ⅱ動詞（混合在一起; 攙雜） mix; blend; mingle
  • 交 : Ⅰ動詞1 （把事物轉移給有關方面） hand over; give up; deliver 2 （到某一時辰或季節） reach （a cert...
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 雜交 : [生物學] hybridize; cross; hybridization; cross breeding
  1. It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent, genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study, genetic diversity in a. polytricha was higher than that in a. auricula : 4 in this study, a. fuscosuccinea had a higher homology to a. auricula than to a. polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a. auricular and a. fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a. auricula as a probe which hybridized with a. auricula and a. fuscosuccinea except a. polytricha, further confirming the veracity of the results from eric - pcr ; 7 in this study, isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity

    本研究中，木耳屬2個種的2個菌株在its區域表現出較高的保守性， 4種限制型內切酶的酶切圖譜沒有顯示出多態性；增加內切酶種類及供試菌株數量，有可能獲得具有多態性的限制性內切酶酶切圖譜； 9本實驗中， its區域的真菌特異性引物與真核生物通用引物對于擴增效果無較大差異，擴增片段長度均為650bp左右； 10根據形態學實驗、 eric - pcr實驗以及southern雜交實驗的結果分析，紫木木耳屬種質資源的遺傳鑒定和遺傳多樣性評價耳極有可能是毛木耳種的一個變種; n .本研究中所用的gutc法是一種適用於木耳屬菌株基因組洲a快速提取的方法; 12 .傳統的形態學分類法和現代的分子生物學分類法，兩者的關系是相輔相成，互為驗證
  2. An analysis of peroxidase and esterase isozyme derived from a generic hybridization among upland rice, barnyard grass and sorghum

    高粱三屬雜交過氧化物酶和酯酶同工酶分析
  3. Chlamys farreri, which belongs to mollusca, bivalvia, pterioidae pectinidae, are widely distributed on the china from donghai sea to bohai sea, korea and japan. this species has been the main aquacultrue shellfish for many years in northchina

    利用同工酶技術，對中國櫛孔扇貝和日本櫛孔扇貝的遺傳差異進行了比較分析，並對它們的正反交後代的酶表型及遺傳型進行了分析，探討了亞種間雜交的機制。
  4. It was showed under the laser scanning confocal microscopy that : for dna level fish, 81 % of the dnas were in the nucleoli and at the periphery of the nucleoli and 19 % in the nucleoplasm ; for rna level fish, 22 % of the rnas were in the nucleoli, 78 % in the nucleoplasm and at the boundary between it and the nucleoli ; for dna - rna level fish, 25 %, 46 % and 29 % of the dnas or rnas were in the nucleoli, at the periphery of the nucleoli and in the nucleoplasm, respectively

    結果如下： dna水平熒光原位雜交結果顯示， 81的dna位於核仁內部及其核仁周邊區域， 19的位於核質中； rna水平熒光原位雜交結果表明， 22的rna位於核仁內， 78的位於核質及與核仁交界處； dna - rna水平熒光原位雜交結果是， 25的dna或rna位於核仁內， 46處于核仁周邊， 29位於核質中。由此推測出， rna聚合酶的轉錄主要發生在核仁及其周邊區域。
  5. 1. because the taxonomic division is rather complex and has been much disputed and revised, in this part, we will review the classification and phylogeny of families, subfamilies and tribes of anseriformes based on morphology, ethology, osteology, mitochondrial and nuclear dna restriction fragment length polymorphism, single - copy nuclear dna hybridization and the sequences of mitochondrial gene analysis referring to the different definition, classification and phylogenetic relationships of the families, subfamilies and tribes of anseriformes. the controversial questions and deficiency in the systematic studies of anseriformes were pointed out

    具體包括以下幾個部分： 1 、針對雁形目鳥類異常復雜的分類狀況及分類上存在的爭議，根據雁形目鳥類的形態學、行為學、骨骼學、角蛋白、線粒體與核dna酶切片段長度多態、單拷貝核dna - dna雜交及線粒體基因dna序列分析等方面的研究，對雁形目鳥類分類中科、亞科和族的劃分及其相互間的系統發生關系進行綜述，分析系統學研究中存在的不足，提出了雁形目鳥類分類中急需解決的問題。
  6. 2. 5 ul of 10 x reaction buffer, 1. 5 ul 25mm mgcl2, 0. 3 ul lomm dntp, 0. 5 ul taqdna polymerase ( 5 u / ul ), and lul ( = 20ng ) of primer were used in per reaction. each reaction was overlaid with one drop of paraffin oil. the initial denaturation step was used at 94 for 1 min 45 sec ; then denatured at 94 for 30 sec, annealed at 37 1 min, extended at iv b 72 for 2 min and repeated the cycle 45 times, at last, extended at 72 ' c for lomin

    等（ 1995 ）利用rapd標記區分美國東部一雜交地帶的蟋蟀的兩個姐妹種， allonemobiusfasciatus和a . socius ，並於1998年使用rapd和異型酶標ic做出了這兩種蟋蟀的基因連鎖圖；國內田英芳、鄭哲民（ 20of ）首次將rapd技術運用於蟋蟀總科的分子系統學研究中，採用2種引物對7種蟋蟀進行了基困組dna多態性研究，並應用upg問a法構建樹狀圖，椎測系統發生關系
  7. Based on the previous studies, the research in this paper was carried out, mainly including two parts as follows : ( 1 ) anammox bacteria and aerobic ammonia oxidizers were detected in situ in 6 sediment samples taken from jiangsu province. molecular techniques, such as fish, pcr, dna cloning and sequencing etc. were used for this purpose. ( 2 ) the continuous cultivation of anammox bacteria from sediment samples were studied, which provides experimental basis for the bioaugamentation of eutrophicated sediment applying anammox process

    本論文在前人研究的基礎上，開展了以下兩個方面的工作： （ 1 ）採用分子生物學技術熒光原位雜交（ fish ） 、多聚酶鏈式反應（ pcr ） 、 dna克隆和測序等對采自江蘇省蘇州市、東太湖、新沂河等6個底質樣品進行了厭氧氨氧化菌和傳統氨氧化菌的原位檢測； （ 2 ）探討了以底質作為接種體進行厭氧氨氧化菌富集培養的可行性，為天然底質環境中厭氧氨氧化過程的強化，富營養化底質微生物修復的可行性提供一定的依據。
  8. Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )

    Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。
  9. The engineering bacterium which carried bcih i - chi and i - glu cdna was pcg - ii. two methods of agrobacterium - mediated and gene gun were used to transformate long ya lillium. the results of pcr analysis and southern dot blotting hybridization demonstrated that the chi a nd glu cdna have been intergrated into host genome. at the same time ; compared agrabactenum - mediated method with gene gun method, the transformation frequency of the former was 16. 7 %, while the latter was 50 %, so gene gun transformation method was suitable for long ya liiliwn

    用攜帶有幾丁質酶基因和- 1 、 3葡聚糖酶基因的工程菌，通過農桿菌介導法和基因槍轉化法轉化龍牙百合，經pcr和點雜交檢測證明外源基因已經整合到植物染色體中。同時對農桿菌介導法和基因槍法進行比較，發現農桿菌介導法的轉化率為16 . 7 ，基因槍法的轉化率為50 ，因此可能基因槍轉化法更適于龍牙百合的遺傳轉化。
  10. So streptomyces sp. fr - 008 is a new strain synthesizing candicidin. nmr studies analysis of the 1h - 1h cosy spectrum allowed us to distinguish some important chemical shifts of the protons in fr - 008b which could be identified as eandicidin d. especially for amino - mycosamine residue, methyl protons and all the protons on the ring could be defined by correlating relation

    利用與糖基合成有關的基因str - de作探針對這兩個菌株的總dna進行了southern雜交，從兩個菌株的總dna中均檢測到一段相同大小的陽性片斷，約6 . 4kd （ bamhi + bglii酶切） 。
  11. Total cellular rna were extracted, 40 micrograms of the total rna were used in the reverse transcription reaction, using superscript ii reverse transcriptase, oligo ( dt ) i8 primers, and cy3 - dctp or cy5 - dctp for the experimental and control group respectively. the labeled cdnas were hybridized to microarrays at 42c for 12 h - 18 h

    提取as _ 2o _ 3作用k562細胞前後的總rna ，用superscript逆轉錄酶逆轉錄成cdna第一鏈，並在逆轉錄的過程中，用cy3 cy5熒光染料分別標記對照組處理組，與自製的k562細胞基因表達譜晶元雜交。
  12. In those instances nuclei as well as cytoplasms came together and the hybrid cells made perfectly functioning enzymes that expressed the genetic code of two types of animal

    在那些例子中，細胞核和細胞質融合在一起，雜交細胞製造出了表達兩種動物遺傳密碼、完美運轉的酶。
  13. There are six systems of biohydrogen production which include biophotolysis, organic degradation of light, hydrogen synthesis via the water - gas shift reaction of photoheterotrophic bacteria, hybrid system of photo - fermentation, anaerobic fermentation and in vitro hydrogen production by hydrogenase

    討論了光合成生物制氫系統、光分解生物制氫系統、水氣交換反應生物制氫系統、光合發酵雜交生物制氫系統和厭氧發酵生物制氫系統、離體氫酶生物制氮系統等6個生物制氫系統。
  14. Herein, we used cloning, sequencing, northern blotting, in situ hybridization, in vitro translation and co - immunoprecipitation, aimed to investigate the expression of wnk genes and the function of wnk4 kinase

    本實驗應用克隆、測序、 northern印跡雜交、原位雜交、體外翻譯和免疫共沉澱技術，旨在研究wnk基因的表達情況及wnk4激酶的功能。
  15. Producing ethanol by breaking down cellulosic materials, such as switch - grass or fast - growing trees like hybrid poplars, with enzymes is promising

    利用酶降解纖維物質? ?例如柳枝稷和速生雜交白楊? ?生產乙醇是很有希望的。
  16. The equipment here may be pipette, electrophoresis system capillary, transblot system, chest / oven molecule hybridization, water bath, dna sequencer, microplate - reader for elisa, microplate washer and so on

    本區所使用的儀器設備可能有加樣器、電泳儀（槽） 、電轉印儀、雜交爐或雜交箱、水浴箱、 dna測序儀、酶標儀和洗板機等。
  17. Unicolor and lilium asiatic hybrids or cultivars in lilium asiatic hybrids were researched with their parents by karyotype, soluble protein, esterase isoenzyme and peroxidase isoenzyme. the results provided identification markers of cytology and biochemistry for hybridization at the early stage in lily breeding programs. the cluster analysis according to similarity coefficient of soluble protein and peroxidase showed that " yellow " and " omega " have the most closest relation in lilium asiatic hybrids

    本研究對百合屬幾個植物的親緣關系進行了可溶性蛋白質、過氧化物酶同工酶分析，同時對亞洲百合雜交系內雜交及其與原種系間的雜交後代進行了核型、可溶性蛋白質、過氧化物酶和酯酶同工酶分析，以期為百合屬植物親緣關系分析提供生化依據，以及為雜交後代的早期鑒定提供細胞學、生化水平的檢測指標。
  18. The pbacph - egf plasmid dna was used to co - transfect bmn cell with the modified bombyx mori baculovirus bm - bacpak dna, which was first linearized by aocl. after two rounds of plaque isolation, the recombinant virus ( named bmbacph - egf ) was further verified with pcr and dot hybridization. the recombinant bmbacph - egf virus was used to infect bmn culture cells at a moi of 10

    重組轉移載體dna與經bsu36i酶切線性化的修飾型病毒bm - bacpakdna共轉染家蠶bmn細胞，兩輪空斑篩選和純化，獲得重組病毒rbmbacph - egf ，經pcr擴增、 dna點雜交等方法鑒定證實重組病毒中已正確插入ph - egf融合基因。
  19. Methods : 1 ) 12h after irradiation, the cell cycle of nih3t3 cells was determined by flow of cytometry and the ratio of alternations in p16 gene exon - 2 was evaluated through pcr - sscp. 2 ) the content of mda, the activities of the sod and gsh - px in the supernatant of nih3t3 cells and the cells were measured by detecting kits immediately after irradiation. 3 ) the level of matrix metalloproteinase - 2 ( mmp - 2 ) in hela cells was detected by western - blotting and dot - blotting 2h after irradiation

    具體方法為： （ 1 ）照射后12h ，收集nih3t3細胞，用流式細胞儀檢測各組細胞的細胞周期， pcr - sscp檢測抑癌基因p16的變化； （ 2 ） nih3t3細胞照射后立即收集細胞和細胞上清，用試劑盒測量mda含量和sod 、 gsh - px的活性並觀察其變化； （ 3 ） western免疫印跡和點雜交法檢測照射2h后的各組hela細胞中基質金屬蛋白酶- 2 （ mmp - 2 ）的表達變化。
  20. The effects of glp - 1 ( 7 - 36 ) nh2 on insulin secretion glp - 1 ( 7 - 36 ) nh2 with the concentration of 2. 5nmol / l, 5. 0nmol / l, l0. 0nmol / l, 20. 0nmol / l, 40. 0nmol / l respectively were added to the medium as different experimental groups, 24 hours later, insulin amount are 68. 76 ? 1. 71 72. 30 ? 3. 13 104. 16 ? 5. 57 110. 98 ?. 29 111. 58 ? 0. 65miu / l respectively, and the insulin account is 55. 53 ?. 63miu / lin the control group. there was no significant difference between the groups with 2. 5nmol / land 5. 0nmol / l glp - 1 ( 7 - 36 ) nh2 respectively ; and there was not significant difference among the groups with lo. onmol / l, 20. 0nmol / l and 40. 0nmol / l glp - 1 ( 7 - 36 ) nh2 respectively. but the difference is significant between experimental groups and control group ( p < 0. 05 ). the data show that with the rising concentration of glp - 1 ( 7 - 36 ) nh2, there is an increasing amount of insulin

    對照組培養液中不含g廿一1 （ 7一36 ） nhz ，實驗組培養液中含有20nmol / lglp一1 （ 7一36 ） nhz ，培養24h后，用0 . 25 %胰蛋白酶消化胰島分散細胞，塗片后利用針對胰島素mrna的寡核甘酸探針進行細胞原位雜交， dab顯色，高清晰度病理圖文分析系統（ highpathologiealimageanalysissystem ， hp認s ）對細胞著色的平均光密度（ mean即tiealdensity ， mod ）量化分析，觀察實驗組和對照組胰島素mrna的表達情況。
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