雜合基因對 的英文怎麼說
中文拼音 [zágějīyīnduì]
雜合基因對
英文
heterozygous gene pair- 雜 : Ⅰ形容詞(多種多樣的; 混雜的) miscellaneous; varied; sundry; mixed Ⅱ動詞(混合在一起; 攙雜) mix; blend; mingle
- 合 : 合量詞(容量單位) ge, a unit of dry measure for grain (=1 decilitre)
- 因 : Ⅰ動詞[書面語] (沿襲) follow; carry on Ⅱ介詞1 [書面語] (憑借; 根據) on the basis of; in accord...
- 對 : Ⅰ動詞1 (回答) answer; reply 2 (對待; 對付) treat; cope with; counter 3 (朝; 向; 面對) be tr...
-
Dsmv is proved as the predominating virus - pathogen on aroid plants from zhejiang province and other regions in china. cdna of dsmv rna 3 " end partial sequence and subgenomic rna promoter region of cucumber mosaic virus ( cmv ) rna3 were used as probes for detection of dsmv and cmv respectively. total rna extracted from field samples were used for rna dot - hybridization
用侵染馬蹄蓮的dsmv3末端序列和黃瓜花葉病毒( cmv )的亞基因組啟動子區互補dna序列為標記探針,對自然感病的天南星科植物進行rna斑點雜交,並結合雙鏈rna分析、病毒提純和形態學觀察,對杭州等地16屬天南星科植物的81個樣品進行了病毒鑒定。Rapd ( random amplified polymorphic dna ) technique was used to study the genetic structure and genetic differentiation of the natural population and the hatchery stock of chlamys farreri. amplifications with 20 10 - base primers under predetermined optimal reaction yielded 153 reproducible amplified fragments ranging from 200 to 3000bp. the mean proportions of polymorphic amplified fragments of natural population and the hatchery stock were 73. 2 % and 70. 6 % respectively, and the mean hetreozygosities of them were 0. 27 and 0. 26 respectively
在對20個野生櫛孔扇貝和20個養殖櫛孔扇貝的基因組dna的檢測中, 20個隨機引物共擴增出153條清晰可分辨的dna片段,片段大小在200 3000bp之間,其中野生種群和養殖群體多態性片段分別為112和108條,多態位點比例分別為73 . 2 %和70 . 6 % ,雜合度分別為0 . 27和0 . 26 。Mattson js, cerutis dr, parrish lc. osteoporosis : a review and its dental implications j. compend contin educ dent, 2002, 23 ( 11 ) : 1004, 1006, 1008
郝永強,戴克服,郭禮和,等.基因重組人生長激素對骨質疏鬆性骨折愈合影響的實驗研究j .中國骨質疏鬆雜志, 2000 , 5 ( 6 ) : 1 - 4The avermetins are a group of closely related macrocylic lactones with exceedingly high activity against helminths and anthropods. this paper review the biosyntheticpathway of the avermectins and the organization of the biosynthetic gene cluster which many groups have analysed and cloned
阿維菌素為一種典型的次級代謝產物,生物合成途徑復雜,現在基本上對每一步合成途徑的基因及其所編碼的酶都有所了解。Abstract : the main characters and seed set of bc1 , bc2 , bc1f2 from ms line back - crossing with the pollen of hybrid rice were studied. the appearance of a large number of self fertile plants in ms line was analyzed. the effect of introgression of restoring genes on ms line purity and hybrid yield in indica and japonica rice was discussed. it was pointed out that “ iso - cytoplasm restorer line ” was seriously reducing the purity of ms line and that hybrid rice was unsuitable for isolation zone in the seed production fields
文摘:研究了雜交稻花粉對母本不育系回交後代群體bc1 、 bc2 、 bc1f2的主要農藝性狀和育性表現,分析了不育系中出現大量自交結實株的原因,討論了恢復基因滲入對秈、粳不育系純度和雜交稻產量的影響,指出「同質恢」對降低不育系純度的嚴重性和雜交稻作制種田隔離區的不合理性。Rapifera, light - independent " akamaru " turnip and light - dependent " tsuda " turnip, were used as test material in this experiment. the expression patterns of pal, chs, chi, as, phya, cop1, cip7 and hy5 were analyzed by northern hybridization. pal, chs, chi and as are the principal structural genes correlated with anthocyanin biosynthesis in anthocyanin biosynthesis pathway ; phya, cop1, cip7 and hy5 are the genes correlated with light signal transmission in anthocyanin biosynthesis pathway
本研究是以光敏感型津田蕪菁和光不敏感型赤丸蕪菁為試材,利用northern雜交技術,對花青素生物合成途徑中與花青素合成相關的主要結構基因pal 、 chs 、 chi和as及光信號傳遞相關的基因phya 、 cop1 、 cip7和hy5的表達進行了檢測。Lastly by using the technique of dot blot hybridization, the genome dna of chlamydia was detected with the probe of momp gene labeled with dig - 11 - dutp by using the way of random primer. the results showed the degree of sensitivity of the probe was 10 pg and other pathogens could not be detected by this probe. by comparing the diagnostic ways of nucleotide probe and fc, the technique of nucleotide probe were proved to have high sensitivity and speci fi city
最後,用地高辛隨機引物法標記成momp基因核酸探針,斑點雜交檢測衣原體基因組dna ,靈敏度可達10pg ,且不能檢出其它病原體的核酸。將核酸探針法與補體結合反應法對衣原體感染的診斷進行比較,初步證明該探針具有較高的敏感性與較強的特異性。The engineering bacterium which carried bcih i - chi and i - glu cdna was pcg - ii. two methods of agrobacterium - mediated and gene gun were used to transformate long ya lillium. the results of pcr analysis and southern dot blotting hybridization demonstrated that the chi a nd glu cdna have been intergrated into host genome. at the same time ; compared agrabactenum - mediated method with gene gun method, the transformation frequency of the former was 16. 7 %, while the latter was 50 %, so gene gun transformation method was suitable for long ya liiliwn
用攜帶有幾丁質酶基因和- 1 、 3葡聚糖酶基因的工程菌,通過農桿菌介導法和基因槍轉化法轉化龍牙百合,經pcr和點雜交檢測證明外源基因已經整合到植物染色體中。同時對農桿菌介導法和基因槍法進行比較,發現農桿菌介導法的轉化率為16 . 7 ,基因槍法的轉化率為50 ,因此可能基因槍轉化法更適于龍牙百合的遺傳轉化。So streptomyces sp. fr - 008 is a new strain synthesizing candicidin. nmr studies analysis of the 1h - 1h cosy spectrum allowed us to distinguish some important chemical shifts of the protons in fr - 008b which could be identified as eandicidin d. especially for amino - mycosamine residue, methyl protons and all the protons on the ring could be defined by correlating relation
利用與糖基合成有關的基因str - de作探針對這兩個菌株的總dna進行了southern雜交,從兩個菌株的總dna中均檢測到一段相同大小的陽性片斷,約6 . 4kd ( bamhi + bglii酶切) 。In this regard theome reasonable judgment as to the extent and frequency of checking based upon a variety of factors such as the complexity of the computer systems
在這一方面,基於多種如計算機系統的復雜性的因素上的核對,其廣度和頻率應該具有合理的判斷結果。Green fluorescent protein ( gfp ) gene was conjugated to the 3 " end of the pap gene in order to screen easily of the transgenic cotton plants. the combined gene was cloned into plant expression vector pbi121 and then transformed. about 5000 seeds of the transgenic cotton were obtained and the some seedlings of the transgenic cotton could give a bright green fluorescence in the dark condition when the cotton seedlings were irradiated with ultraviolet rays
為了便於轉基因棉花後代的篩選,在pap基因的3 』端融入了綠色熒光蛋白gfp )基因,然後將融合基因克隆在植物表達載體pbi121上,再進行遺傳轉化,得轉基因棉花種子5000餘粒,將種子播種長到于葉展開時,先在黑暗中用紫外燈照射,查找表現綠色熒光的幼苗,然後再用地高辛( dig )標記的pap基因特異性探針對這些棉花進行點雜交,最後發現有8株棉花表現陽性反應,說明pap基因的確己經轉到了棉花的基因組中,其棉花黃萎病的抗性鑒定正在進行之中。The env protein deduced from env gene encodes the hydrophilic surface protein ( su ) and the hydrophobic transmembrane domain ( tm ) that determine the specific interaction between virus particles and cell surface receptors during retroviral entry. the su of retroviruses is a highly variable genetic element, containing receptor binding sites and major antigenic determinants. exjsrv - specific dna probes were derived. by using these dna probes in tissue hybridization. we successfully identified jsrv mrna expression and proviruses dna in sheep lung tissues infected with jsrv and control group has no postive signals, validating the use of exogenous virus - specific dna probes in the analysis of oncogenic proviral integration sites and identification of integrated exogenous proviral sequences
用地高辛隨機引物法標記exjsrv特異的env片段,制備探針,原位雜交檢測spa肺組織中的rna及前病毒dna ,結果表明spa患羊肺組織內有jsrvenv基因mrna的表達,同時也檢測到了前病毒dna ,而相應的陰性對照卻無陽性信號,證實外源性病毒特異的dna探針在致瘤性前病毒的整合位點和整合的外源性前病毒的檢測中具有可信度。$ mx # ( 2bo2 ) ffr $ $ htet # $ nq9i & kk $ 4n8 $ $ 4ta scf mana $ ismffs whether s1eep deprivation cou1d induce neuron pro1iferation has not got definite conclusion so far. in the present research, we performed small pedestal s1eep deprivation method to remove rapid eye movement s1eep ( rems ). we observed the neuron pro1iferation and differential in immunohistochemistry and doub1e 1abeling iariunohistochemistry staining, and the expression of stem ce11 factor mrna in situ hybridization technique after s1eep deprivation in rat hippocampus
本研究應用細胞增殖標記brdu cna免疫組織化學方法結合雙重標記兔疫組織化學技術,觀察睡眠剝奪對成熟大鼠腦內神經元增殖及分化的影響,應用原位雜交技術觀察了睡眠剝奪大鼠海馬神經細胞scf基因inrna表達的變化,對睡眠剝奪引起海馬神經細胞增殖的分子機製作了初步探討。The results indicated that opposite maize had widespread heterosis like alternative maize ; yield advantage of opposite f1 ( alternative alternative ) was better than that of f1 ( opposite opposite ) and f1 ( alternative opposite or reverse ) ; general combining ability of yield characters was decreased by opposite characters transfer maize, but alternative maize with opposite genes has high general combining ability, so that on the opposite maize breeding, alternative inbred lines with different opposite genes can be parents for breeding hybrid lines ( alternative alternative ) and hybrid varieties with more seeds and high yield can be obtained
結果表明:對生玉米同互生玉米一樣具有普遍的雜種優勢,在產量性狀上f1對生株優勢互互組合大於對對組合和對互(互對)組合;對生性狀的轉育對產量性狀的一般配合力有降低效應,而含有對生基因的互生玉米具有較高的一般配合力效應,因此,在對生玉米育種中可以利用含有不同對生基因的互生自交系作親本來組配雜交組合(互互) ,從而獲得較高的制種產量和高產的對生雜交種。In order to select male nucleo - sterile new genotype, a tentative idea was put forward for the nucleo - male sterility to attach a tps ( thermo - photoperiod sensitivity ) and a selection strategy of combination of selection and identification, at the same time, the spring and summer sowing method were used to provide different environment conditions of appraising sterility and tps. the results indicated that ( 1 ) sterility could be appraised under the spring sowing environment and tps could be appraised under summer sowing environment. ( 2 ) under spring sowing environment, sterility could be selected, but not maintained. thereby, lines selected could only be selected as recorded selection method in the experiment. ( 3 ) and then, selection was carried out from spring sowing line selected into summer sowing in same line with tps to select plants. these plants through the intercrossing selection had been combined with sterility and tps. in this way, a new selection protocol for selection sterile line with tps was formed. it mainly involves the spring and summer sowing method, recorded selection method and the intercrossing selection method
為了選育新類型玉米雄性核不育系,提出了為玉米核不育性添加溫光敏感性的設想和選擇與鑒定相結合的策略,同時應用分期播種的方法為作物提供不同的生長和發育的環境條件,以鑒定玉米雄穗的育性變化和對不同環境條件溫光的反應.研究結果表明,春播環境下可鑒定和選擇玉米的不育性,夏播環境下可鑒定和選擇其溫光敏感性.針對玉米核不育性難以找到保持系的特性,結合兩種播期選擇兩種性狀.但春播環境下選擇的不育性群體難以通過選擇單株來保持其不育性,為記錄性群體選擇.通過從春播入選的雜合不育性優良株系群體轉移到其對應的夏播溫光敏感性入選的同一優良群體中進行優良單株選擇,能逐漸使不育性和溫光敏感性相結合而選育出純合溫光敏不育系.這種新的選擇程序主要包括應用分期播種法、記錄性選擇法和春夏兩季交叉式選擇法,使含有不育基因的可育株系逐步累積不育基因並增加了溫光敏感性而育成玉米溫光敏不育系Abstract : plant responses to salt stress via a complex mechanism, including sensing and transducing the stress signal, activating the transcription factors and the corresponding metabolizing genes. since the whole mechanism is still unclear, this review emphasize the biochemical events during the plant adaptation to salt stress referring to an index of importance : the homeostasis in cytoplasm, the biosynthesis of osmolytes and the transport of water. most of these biochemical events were elucidated by study of halophyte and salt - sensitive mutations, also many important genes involved were cloned and used to generate stress - tolerance phenotypes in transgenic plants. on the other hand, about the molecular mechanism in signal transduction, the research of arabidopsis mutations and yeast functional complementation provided helpful traces but not full pathway
摘要植物對鹽脅迫的耐受反應是個復雜的過程,在分子水平上它包括對外界鹽信號的感應和傳遞,特異轉錄因子的激活和下游控制生理生化應答的效應基因的表達.在生化應答中,本文著重討論負責維持和重建離子平衡的膜轉運蛋白、滲調劑的生物合成和功能及水分控制.這些生理生化應答最終使得液泡中離子濃度升高和滲調劑在胞質中積累.近年來,通過對各種鹽生植物或鹽敏感突變株的研究,闡明了許多鹽應答的離子轉運途徑、水通道和物種特異的滲調劑代謝途徑,克隆了其相關基因並能在轉基因淡水植物中產生耐鹽表型;另一方面,在擬南芥突變體及利用酵母鹽敏感突變株功能互補篩選得到一些編碼信號傳遞蛋白的基因,這些都有助於闡明植物鹽脅迫應答的分子機制。In addition, the well retained stability and integrity of cell membrane of boea leaves might also be an important mechanism which make them resurrect well. by using mrna differential display, 5 desiccation sensitive cdnas, 52 desiccation - induced cdnas, 21 up - regulated cdnas, 14 down - regulated cdnas and 16 phosphate induced cdnas were obtained. the cloning, sequencing, homological blasting and northern blotting results of 5 desiccation - induced cdnas and 3 phosphate induced cdnas implied that signal transduction induced by desiccation, regulatory gene cascades and functional genes such as g protein, protein kinase, vp3 - and mad3 - like genes might be involved in dehydration in the resurrection plant boea hygrometrica
對其中5個脫水特異誘導表達牛耳草光合作廠j的脫水保護和復甦機理的cdna (包括可能與復甦能力有關的cdna )和3個磷酸鹽處理誘導表達的cdna進行克險測序、同源性探測和northern雜交檢測表明,牛耳草脫水過程中誘導表達的基因可能涉及到脫水脅迫的信號轉導「蛋白、蛋白激酶等) 、調節基因的級聯作用( vp3 , mad3樣基因等) 、結構基因產物調節細胞結構(包括細胞質膜)在脫水脅迫中的穩定性等。Facing to manufacturing system, this paper analyzed its characters and evolvement of production management modes, demonstrated that lean production and human - based theory were practical ways to reform our manufacturing systems fit for the reality. a division method was raised to separate the job - scheduling problem into shop and station types, and emphasis was pressed on the study of partheno - genetic algorithm ( pga ), a revised genetic algorithm, to calculate this problem. a new kind of gene joint - move algorithm was also raised
針對製造系統,分析其生產管理模式的特點和變遷,論證了精益生產和以人為本的思想是適合現階段我國實際的製造系統管理模式改造途徑,對其中的主要具體問題?作業計劃調度提出shop (車間)和station (工作站)型兩分法,求解上重點研究了遺傳演算法的改進型式?單染色體遺傳演算法( pga )的基本理論,提出一種基因聯合移位運算元,證明了演算法的有效性與優越性,研究了如何將pga應用於製造系統作業調度這一復雜的組合優化問題,針對生產中總時間最短和jit兩種生產性能指標,給出了具體的解決方案及運算過程。In this dissertation, the plasmids containing 5s promoter were transfected into cho cells and the transcription sites of rna polymerase and its transcripts were detected by fluorescence in situ hybridization to dna and rna, respectively
本實驗以中國倉鼠卵巢細胞( cho )為實驗材料,利用基因轉染、熒光原位雜交並結合激光共聚焦顯微鏡觀察的方法,在dna和rna水平上分別對rna聚合酶的轉錄位點和轉錄子的分佈進行了檢測。The cdna expression library that contains no intron theoretically include all expressed genes and conserve resource genes permanently. lt can be used to find out and clone some genes which can express particular protein with modern molecular biological techniques, such as immunological screening, drug - prob screening, southern et al. lt is very important to study the life nature of plasmodium falciparum in molecular level. with the developments of these studies, the drug - resistant mechanism of the plasmodium falciparum and the genes of some specific medicine binding protein can be made well - known. at the same time, the researches will do good to explain the mechanism of some specific medicines in order to design and screen new anti - malaria drugs
建立cdna表達文庫在一次永久保存基因資源的同時,可以利用功能篩選、免疫學篩選、藥物探針篩選、 southern雜交和大規模序列測定等現代分子生物學技術尋找特異性活性蛋白基因,進而克隆和表達這些基因,對從核酸及蛋白質等分子水平研究瘧原蟲的生命活動規律,對揭示其抗藥性分子機理,搞清某些特效藥物結合蛋白的基因及此類藥物的作用機制,對新型抗瘧藥物的合理設計及篩選都具有極其重要的現實意義。分享友人