雜合基因 的英文怎麼說
中文拼音 [zágějīyīn]
雜合基因
英文
heterozygous genes-
When the two alleles are not identical, they are said to be heterozygous.
當兩個等位基因不同時,稱之為雜合性的。Dsmv is proved as the predominating virus - pathogen on aroid plants from zhejiang province and other regions in china. cdna of dsmv rna 3 " end partial sequence and subgenomic rna promoter region of cucumber mosaic virus ( cmv ) rna3 were used as probes for detection of dsmv and cmv respectively. total rna extracted from field samples were used for rna dot - hybridization
用侵染馬蹄蓮的dsmv3末端序列和黃瓜花葉病毒( cmv )的亞基因組啟動子區互補dna序列為標記探針,對自然感病的天南星科植物進行rna斑點雜交,並結合雙鏈rna分析、病毒提純和形態學觀察,對杭州等地16屬天南星科植物的81個樣品進行了病毒鑒定。Rapd ( random amplified polymorphic dna ) technique was used to study the genetic structure and genetic differentiation of the natural population and the hatchery stock of chlamys farreri. amplifications with 20 10 - base primers under predetermined optimal reaction yielded 153 reproducible amplified fragments ranging from 200 to 3000bp. the mean proportions of polymorphic amplified fragments of natural population and the hatchery stock were 73. 2 % and 70. 6 % respectively, and the mean hetreozygosities of them were 0. 27 and 0. 26 respectively
在對20個野生櫛孔扇貝和20個養殖櫛孔扇貝的基因組dna的檢測中, 20個隨機引物共擴增出153條清晰可分辨的dna片段,片段大小在200 3000bp之間,其中野生種群和養殖群體多態性片段分別為112和108條,多態位點比例分別為73 . 2 %和70 . 6 % ,雜合度分別為0 . 27和0 . 26 。Mattson js, cerutis dr, parrish lc. osteoporosis : a review and its dental implications j. compend contin educ dent, 2002, 23 ( 11 ) : 1004, 1006, 1008
郝永強,戴克服,郭禮和,等.基因重組人生長激素對骨質疏鬆性骨折愈合影響的實驗研究j .中國骨質疏鬆雜志, 2000 , 5 ( 6 ) : 1 - 4Shaking flask experiments and hplc analyses showed that the four mutants no longer produced the toxic oligomycin, and only made four components of avermectins b, which were avermectin b1a, b1b, b2a, b2b. the yields of avermectins b in these mutants were separately equal to those in cz8 - 73. this revealed that olma genes deletion did n ' t affect the biosynthesis of avermectins
將4株經southern雜交驗證正確的基因缺失突變株進行搖瓶發酵和hplc檢測,發現4個突變株均不再產生寡黴素而僅產阿維菌素b組分,阿維菌素的總產量和b1的產量與出發菌株相當,說明寡黴素pks基因簇的缺失並不影響阿維菌素的生物合成。Gosden j, lawson d. rapid chromosome identification by oligonucleotideprimed in site dna synthesis ( prins ) [ j ]. hum mol genet, 1994, 3 : 931
趙曉嵐,葉國玲,楚雍烈,等.產前篩查和基因多態性位點在唐氏綜合征產前診斷中的應用[ j ] .中國優生與遺傳雜志2003 , 11 ( 2 ) : 49The avermetins are a group of closely related macrocylic lactones with exceedingly high activity against helminths and anthropods. this paper review the biosyntheticpathway of the avermectins and the organization of the biosynthetic gene cluster which many groups have analysed and cloned
阿維菌素為一種典型的次級代謝產物,生物合成途徑復雜,現在基本上對每一步合成途徑的基因及其所編碼的酶都有所了解。It revealed that these two transformants were heterozygote, and the foreign gene inserted just to on locus in the plant genome. as the observed ratio of progeny of two transformants which on selfing was 3 : 1, it was proved to be heterozygote for two transformants
其中2棵抗性株自交後代的遺傳分析表明,真空滲入法所得的轉化株為雜合體,且其分離比符合3 : 1的單基因分離規律。The callipyge phenotype in sheep is an inherited muscular hypertrophy that affects only heterozygous individuals who receive the clpg mutation from their father
只有遺傳了來源於父本的clpg突變的雜合子個體才能表達這一表型,因此推測這一性狀受印記基因的控制。The results show that the genetic diversity of filial generations is higher than self - bred progeny ’ s , the hybrid vigor has manifested at metabolization level 。 the proportion of polymorphic loci ( p. 99 ) in f1 generations was 30. 77 - 36. 36 % , the mean observed heterozygosity ( ho ) of jmcf ( japanese male chinese female ) was the highest with 0. 1727, the rest populations were : jfcm ( japanese female chinese male ) 0. 1536 、 cp 0. 1273 and jp 0. 1055 , the mean effective number of allels per locus in f1 generations : cp ’ s was the highest with 1. 7318 , jp ’ s was the lowest with 1. 6718 , the filial generations ’ values were in the midle of the cp ’ s and jp ’ s and between them there was no brightly difference, with 1. 6955 ( jfp ) and 1. 6927 ( jmp )
各組的平均雜合度預期值也表現出該趨勢,即:日自交組最低,為0 . 1786 ,日雄中雌雜交組的平均雜合度最高,為0 . 1964 ,日雌中雄雜交組和中自交組分別是0 . 1891和0 . 1865 。 hardy - weinberg平衡下遺傳偏離指數也是雜交組的小於自交組的,平均每個位點的等位基因有效數目中自交組最高為1 . 7318 ,日自交組最低為1 . 6718 ,兩雜交組群體的這一指數相差不多,分別是1 . 6955和1 . 6927 ,位於兩親本的中間水平。Abstract : the main characters and seed set of bc1 , bc2 , bc1f2 from ms line back - crossing with the pollen of hybrid rice were studied. the appearance of a large number of self fertile plants in ms line was analyzed. the effect of introgression of restoring genes on ms line purity and hybrid yield in indica and japonica rice was discussed. it was pointed out that “ iso - cytoplasm restorer line ” was seriously reducing the purity of ms line and that hybrid rice was unsuitable for isolation zone in the seed production fields
文摘:研究了雜交稻花粉對母本不育系回交後代群體bc1 、 bc2 、 bc1f2的主要農藝性狀和育性表現,分析了不育系中出現大量自交結實株的原因,討論了恢復基因滲入對秈、粳不育系純度和雜交稻產量的影響,指出「同質恢」對降低不育系純度的嚴重性和雜交稻作制種田隔離區的不合理性。Guan xiaohong ( 1991 ) established a cell line of the monoclonal anti - idiotypic antibody ( anti - id ) np30 of schistosoma - 6 - japonicum, whose isotype was identified to be igm and which was testified to be internal image of gaa
分別以日本血吸蟲單克隆抗獨特型抗體wbo的雜交瘤細胞株總基因組dna和其提取rna進行rticr合成的cdna第一鏈為模板,擴增v 。 、 v 。There is also a general lowering of vigor in inbred stock ( inbreeding depression ), which is especially pronounced amongst normally outbreeding populations
而雜合型出現的頻率降低,因而降低了種群基因的潛在可變性。Rapifera, light - independent " akamaru " turnip and light - dependent " tsuda " turnip, were used as test material in this experiment. the expression patterns of pal, chs, chi, as, phya, cop1, cip7 and hy5 were analyzed by northern hybridization. pal, chs, chi and as are the principal structural genes correlated with anthocyanin biosynthesis in anthocyanin biosynthesis pathway ; phya, cop1, cip7 and hy5 are the genes correlated with light signal transmission in anthocyanin biosynthesis pathway
本研究是以光敏感型津田蕪菁和光不敏感型赤丸蕪菁為試材,利用northern雜交技術,對花青素生物合成途徑中與花青素合成相關的主要結構基因pal 、 chs 、 chi和as及光信號傳遞相關的基因phya 、 cop1 、 cip7和hy5的表達進行了檢測。Lastly by using the technique of dot blot hybridization, the genome dna of chlamydia was detected with the probe of momp gene labeled with dig - 11 - dutp by using the way of random primer. the results showed the degree of sensitivity of the probe was 10 pg and other pathogens could not be detected by this probe. by comparing the diagnostic ways of nucleotide probe and fc, the technique of nucleotide probe were proved to have high sensitivity and speci fi city
最後,用地高辛隨機引物法標記成momp基因核酸探針,斑點雜交檢測衣原體基因組dna ,靈敏度可達10pg ,且不能檢出其它病原體的核酸。將核酸探針法與補體結合反應法對衣原體感染的診斷進行比較,初步證明該探針具有較高的敏感性與較強的特異性。Molecular examination and biological observation of transformed plants by growing resistant germinations we got 30 transformed plants. pcr and spot blotting hybridization analysis indicate that hsp70 anti - sense cdna had been integrated into tobacco genome. through observation and measurement we found 12 completely sterile, 9hyper - sterile, 3 partially sterile and 6 fertile and significant differences between them in stamen, height, and fruit weight
Pcr和點雜交分析表明, hsp70正、反義。 dna己經整合到煙草的基因組中。在進行田間生物學性狀觀察時, 30株轉基因植株中有12株表現出完全不育, 9株高度不育, 3株部分不育和6株可育;不育植株在雄蕊、株高和果實重量等方面都存在明顯的差異。The engineering bacterium which carried bcih i - chi and i - glu cdna was pcg - ii. two methods of agrobacterium - mediated and gene gun were used to transformate long ya lillium. the results of pcr analysis and southern dot blotting hybridization demonstrated that the chi a nd glu cdna have been intergrated into host genome. at the same time ; compared agrabactenum - mediated method with gene gun method, the transformation frequency of the former was 16. 7 %, while the latter was 50 %, so gene gun transformation method was suitable for long ya liiliwn
用攜帶有幾丁質酶基因和- 1 、 3葡聚糖酶基因的工程菌,通過農桿菌介導法和基因槍轉化法轉化龍牙百合,經pcr和點雜交檢測證明外源基因已經整合到植物染色體中。同時對農桿菌介導法和基因槍法進行比較,發現農桿菌介導法的轉化率為16 . 7 ,基因槍法的轉化率為50 ,因此可能基因槍轉化法更適于龍牙百合的遺傳轉化。Green fluorescent protein ( gfp ) gene was conjugated to the 3 " end of the pap gene in order to screen easily of the transgenic cotton plants. the combined gene was cloned into plant expression vector pbi121 and then transformed. about 5000 seeds of the transgenic cotton were obtained and the some seedlings of the transgenic cotton could give a bright green fluorescence in the dark condition when the cotton seedlings were irradiated with ultraviolet rays
為了便於轉基因棉花後代的篩選,在pap基因的3 』端融入了綠色熒光蛋白gfp )基因,然後將融合基因克隆在植物表達載體pbi121上,再進行遺傳轉化,得轉基因棉花種子5000餘粒,將種子播種長到于葉展開時,先在黑暗中用紫外燈照射,查找表現綠色熒光的幼苗,然後再用地高辛( dig )標記的pap基因特異性探針對這些棉花進行點雜交,最後發現有8株棉花表現陽性反應,說明pap基因的確己經轉到了棉花的基因組中,其棉花黃萎病的抗性鑒定正在進行之中。The pbacph - egf plasmid dna was used to co - transfect bmn cell with the modified bombyx mori baculovirus bm - bacpak dna, which was first linearized by aocl. after two rounds of plaque isolation, the recombinant virus ( named bmbacph - egf ) was further verified with pcr and dot hybridization. the recombinant bmbacph - egf virus was used to infect bmn culture cells at a moi of 10
重組轉移載體dna與經bsu36i酶切線性化的修飾型病毒bm - bacpakdna共轉染家蠶bmn細胞,兩輪空斑篩選和純化,獲得重組病毒rbmbacph - egf ,經pcr擴增、 dna點雜交等方法鑒定證實重組病毒中已正確插入ph - egf融合基因。The chimeric gene was introduced into arabidopsis through the method of vacuum infiltration which is widely used in arobidopsis tansformation. transgenic plants were screened by means of kanamycin resistance and further identified by genomic pcr and western blotting, through the phenotype observation of transgenic plants, the speculation that apoplast calmodulin may play a certain role in regulating the development and growth of plants was made
最後,融合基因被插入二元載體plbj21 ,通過電擊法轉入農桿菌gv3101 ,真空滲入法轉入了擬南芥,轉基因擬南芥通過kan抗性篩選而得到,並進一步進行了基因組pcr鑒定和western雜交的鑒定。分享友人