離子交換層析 的英文怎麼說

中文拼音 [lízijiāohuàncéngxī]
離子交換層析 英文
ion exchange chromatography
  • 離 : Ⅰ動詞1 （離開） leave; part from; be away from; separate 2 （背離） go against 3 （缺少） dispens...
  • 子 : 子Ⅰ名詞1 （兒子） son 2 （人的通稱） person 3 （古代特指有學問的男人） ancient title of respect f...
  • 交 : Ⅰ動詞1 （把事物轉移給有關方面） hand over; give up; deliver 2 （到某一時辰或季節） reach （a cert...
  • 換 : 動詞1. （給人東西同時從他那裡取得別的東西） exchange; barter; trade 2. （變換; 更換） change 3. （兌換） exchange; cash
  • 層 : i 量詞1 （用於重疊、積累的東西 如樓層、階層、地層） storey; tier; stratum 2 （用於可以分項分步的...
  • 析 : Ⅰ動詞1. （分開; 散開） divide; separate 2. （分析） analyse; dissect; resolve Ⅱ名詞（姓氏） a surname
  • 離子 : [物理學] ion
  1. Strain bl21, and gene expression was induced by iptg. the target proteins were directed into the periplasmic space by the staphylococcal protein a signal sequence preceding the rgd - hirudin gene. using ion exchange chromatography and gel filtration chromatography, the chimera proteins were purified, and both of them showed a single band in tricine - sds - page. the results of activity analysis suggested that these two chimera proteins not only have antithrombin activities, but gain platelet aggregation inhibitory activities as well

    通過離子交換層析和凝膠過濾層分別對兩種嵌合體蛋白進行純化，純化產物在tricine - sds - page中都顯示為單一條帶。活性分析結果表明兩種嵌合體蛋白在保留水蛭素抗凝血酶活力的同時，還呈現抗血小板聚集活性。
  2. 6 - phosphogluconate dehydrogenase ( 6 - pgadase, ec 1. 1. 1. 44 ) was isolated by homogenate, ammunium sulfate fractionation, deae - sepharose chromatography, blue - sepharose affinity chromatography and gel filtration with sephadex g - 200 from bacillus subtilis, and some properties of the enzyme had been studied. a 113. 8 - fold purification was obtained with a 8. 2 % yield. the purified enzyme moved as a single electrophoretic band in page

    將枯草芽孢桿菌超聲波破壁后的粗提取物進行分段鹽析、 deae - sepharose陰離子交換柱層析， blue - sepharosecl - 6b特異結合柱層析和sephadexg - 200凝膠過濾等純化步驟，得到聚丙烯酰胺凝膠電泳為單一蛋白區帶，比活為1 . 46u mg的酶制劑。
  3. This enzyme was different with the ones reported in the past. a phosphatase was isolated from the chloroplast thylakoid membrane of ipomoea aquatica, by nacl extration, ammonium sulfate precipitation, ion - exchange chromatography and hydrophic chromatography through butyl - toyopearl 650m column

    使用nacl抽提、硫酸銨分步沉澱、離子交換和butyl - toyopearl650m疏水柱層析等方法，從蕹菜葉綠體類囊體膜中分離純化到一種蛋白磷酸酯酶。
  4. Based on the extensive studies of subtilisin - like protease ( prl ) of metarhizium anisopliae, extracellullar serine protease is suggested to be a key enzyme involved in the fimgal penetration to invertebrates. the investigation of serine protease in the nematode infected by owvtl may help to understand the mechanism of nematophagous fimgi as biological control agents. a 3l kda serine protease was isolated and purified from the liquid culture of h rhossiliensis owvtl challenged with nematode panagrellus redivivus

    本研究利用線蟲誘導下owvt - 1菌株液體發酵，通過粗分級分離、離子交換層析和凝膠過濾層析分離提純了一個分子量為31kda的絲氨酸蛋白酶，生物學測定表明其對大豆胞囊線蟲二齡幼蟲具有致死作用，同時測定了該酶理化特性，酶活力在75附近酶活力最高，隨著ph的增加酶的穩定性升高，與膽堿酯酶具有相似的ph曲線，對特異性底物aape （ suc - ala - ala - pro - glu - pna ）具有作用， ssi和ci - 2抑制該酶的活性。
  5. The ps ii native fractions ( 20 % and 30 % ) were loaded onto a deae column. the fraction eluted with 150 mm nacl was presented dcip reduction activity and was highly depleted in chi c and xanthophylls, and as such could be considered a ps ii core complex

    對于有dcip光還原活性的20和30層帶的復合物，進一步deae離子交換層析純化。 150mmnacl洗脫純化后的樣品經過熒光激發光譜測定發現，已經去除了葉綠素c和墨角藻黃素，並且仍然具有dcip的光活性，分析是ps核心復合物。
  6. The xerocomus spadiceus lectin, xsl, was isolated from extracts of fruiting bodies of the mushroom xerocomus spadiceus using a procedure that involved ( nh4 ) 2so4 precipitation, anion exchange chromatography on deae - cellulose, affinity chromatography on affi - gel blue gel, cation exchange chromatography on cm - cellulose, and gel filtration by fast protein liquid chromatography on superdex 75

    從磚紅絨蓋牛肝菌（ xerocomusspadiceus ）子實體粗提物中，經過deae -纖維素陰離子交換層析、 affi - gelbluegel親和層析、 cm -纖維素陽離子交換層析和superdex75fplc凝膠過濾，純化了磚紅絨蓋牛肝菌凝集素。
  7. Medium experiments were arranged under uniform design, and then an optimum medium was got accordingly. the culture liquid was centrifugalized at 3, 500r / min for 30min, then ammonium sulfate was added into the supernatant to a final concentration of 30 % to precipitate the others

    通過硫酸銨分級沉澱、 deaesephadexa - 50陰離子交換凝膠層析和sephadexg - 75凝膠柱層析對發酵液進行分離和純化，並得到電泳純的酶。
  8. Conentional methods such as fractionation of complex clinical samples by ionic exchange chromatography and new methods such as enriching low abundant proteins by affinity capture with a combinatorial library of ligands14 proide much needed tools for processing complex biological and clinical samples for proteomics research

    傳統（如對復雜臨床樣品通過離子交換層析進行分離）和現代的方法（如通過親和捕獲與配體庫的組合富集低豐度蛋白）為處理用於蛋白質組學研究的復雜的生物學與臨床樣品提供了極需的工具。
  9. Calmodulin - dependent cyclic nucleotide phophodiesterase was prepared from bovine brain by two - step deae - cellulose column chromatography

    摘要通過兩次離子交換柱層析，從牛腦中制備鈣調素依賴性的環核苷酸磷酸二酯酶。
  10. Purification and characterization of phytase from a. niger an 01001 a. niger an01001 was inoculated on solid media and cultivated at 30 for 5 days. proteins were extracted from solid - state fermentation with 50mm acetate buffer ( ph5. 5 ). the molecule weight of the phytase protein was determined as about 78kd by sds - page. the purification procedures include ammonium sulfate precipitation, deae - cellulose ion - exchange chromatography, gel electrophoresis and electroelution

    3 .植酸酶的分離純化及其性質研究黑麴黴ano1001經固體發酵，用緩沖液抽提后，經硫酸按沉澱， deae一纖維素離子交換層析，聚丙烯酞胺凝膠電泳和電洗脫等純化步驟獲得的植酸酶，用sds一page檢測為一條均一譜帶，其分子量約為78kd 。
  11. Fusion expression of m - centrin in e. coil bl21 was performed by induction of fptg. fusion protein was digested by ppase and was purified by gst chelating affinity chromatography and ion exchange chromatography. the final products were checked by sds - page gel

    融合蛋白gst - m - centrin菌體經過超聲波裂解后得到的上清夜經過gst親和層析後用prescissionprotease （ ppase ）酶切，酶切產物再次經過gst親和層析和hitrapq陰離子交換層析兩步柱層析純化后，得到純度較高的的m - centrin 。
  12. Then bacteria were collected by centrifugation and split by ultrasonic method. the purified products were analyzed by tris - tricine sds - page. the immuno - activity of the recombinant proteins were analyzed by western blot

    將離心收集到的菌體超聲破壁，離心分離，收集上清液，進行離子交換層析和分子篩分離純化。
  13. Under these conditions the fibrinolytic activity of the supernatant can reach 820 urokinase units per milliliter broth. ba - dfe was purified from the supernatant of b. amyloiquefaciens dc - 4 culture broth by ammonium sulfate precipitation, ion - exchange chromatography on cm - and deae - sepharose fast flow, hydrophobic interaction chromatography on phenyl sepharose 6 fast flow and gel filtration on sephadex g - 50. the purified enzyme displayed thermophilic, hydrophilic and strong fibrinolytic activity

    通過硫酸銨分級沉澱、 cm - sepharosefastflow和deae - sepharosefastflow離子交換層析、 phenylsepharose6fastflow疏水層析和sephadexg - 50凝膠過濾等方法，從解澱粉芽孢桿菌dc - 4的發酵液中分離純化出電泳純的ba - dfe 。
  14. The enzyme activity in fermentation liquid could be inhibited by pmsf and dfp. the fermentation liquor also showed good dehairing activity. the alkaline protease ( named dhap, dehairing alkaline protease ) in the fermentation liquid was purified with hydrophobic interaction chromatography, ion exchange and gel filtration

    通過cm - sepharosefastflow離子交換層析， deae - sepharosefastflow離子交換層析， sephacryls - 100 ， sephacryls - 200凝膠過濾層析，疏水層析等純化步驟對短小芽孢桿菌發酵液中的堿性蛋白酶進行了純化。
  15. Polyethylene glycol - accompanied ion - exchange chromatography to purify recombinant hepatitis b virus surface antigen

    聚乙二醇伴隨式離子交換層析分離重組乙肝病毒表面抗原
  16. An active metabolite was obtained by purification with precipitated by ethanol, sephadex g - 25 gel, deae - cellulose ion exchange resin and silica gel column chromatography

    經乙醇（ 95 ）沉澱、 sephadexg - 25凝膠層析、 deae -纖維素離子交換層析和硅膠層析純化，得到抑制黑麴黴生長的單一組分。
  17. But the whole level of serum titer in combined vaccine group was higher than others. igg was extracted by salting out with ammonium sulfate and purified by ion exchange chromatography with deae cellulose

    對分離到的血清用飽和硫酸銨鹽析法提取igg ，並用deae纖維素離子交換層析法對提取的igg進行純化。
  18. The purified enzyme had a specific activity of 68. 6 u / mg protein. overproduction of pga was often limited by translocation and / or periplasmic processing steps, subsequently resulted in intracellular accumulation of various types of pga precursors and then formed inclusion bodies in the cytoplasm and / or periplasm

    經deae - sepharosecl6b離子交換層析和butyl - sepharosecl4b疏水層析，即可得純度提高20倍、比活為68 . 6u mg的青霉素g酰化酶，兩步純化的總得率達91 。
  19. We have sifted 103 medicinal plants, roughly identified 17 plants might contain antifungal proteins. antifungal protein was purified from cassia sophera linn, by extraction, fraction with ( nh _ ( 4 ) ) _ ( 2 ) so _ ( 4 ), cation - exchange chromatography of cm - sepharose ff xk 26, the first cation - exchange chromatography of mono s and the second one, followed by gel filtration of superose 12hr

    對茳芒決明進行了抗菌蛋白的分離純化：經粉碎、磷酸緩沖液浸提、硫酸銨沉澱、 cm - sepharoseffxk26陽離子交換層析、兩次monos陽離子交換層析、 superose12hr分子篩層析可得到具抗真菌活性的蛋白。
  20. Methods 1 mr1 hybridoma was cultured in hollow fibre system. culture supernatant was harvested, then precipitated by half - saturated ammonia sulphate. mfm was purified by deae - sephacel ion exchange chromatography. mri concentration was measured by elisa

    硫酸錢半飽和沉澱， oeae陰離子交換層析分離純化抗co40l單克隆抗體mri 。 pbs透析后， 505一隊ge檢測純化抗體的純度， eusa測定抗體濃度。
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