離子過篩 的英文怎麼說
中文拼音 [líziguòshāi]
離子過篩
英文
ionic sieving-
It can used to separate normal paraffin from branched hydrocarbon and cricoid hydrocarbons through selective adsorption
5a分子篩吸附劑可通過選擇性吸附過程而從支鏈烴及環狀烴中分離出正構烷烴。Metallophalocyanines ( mpcs ) are a kind of centrosymmetric planar organo - metallic molecules with an extensively delocalized two dimensional conjugated - electron system which show a relatively large third order optical nonlmearity, varying upon central metal atom substitution and other factors. other interesting properties of this molecule and many of its derivative products are their versatility, architectural flexibility and high environmental stability, which are very important requirements to implement photo - electronic applications
因其骨架結構特徵和可通過選擇中心離子、軸向配體和在酞菁環上引入功能性取代基等方法進行分子篩選與組裝得到具有特殊的物理化學性質和光、電、催化等功能的材料，而引起化學家和材料學家的濃厚興趣。Then enzyme was purified with a deae - cellulose ( 5. 5x50cm ) column, a toyopearl hw - 65 ( 5. 5 x 50cm ) column and a sephadex g - 200 ( 5. 5 x 80cm ) column. finally, the enzyme was purified for 10 folds with the recovery of 17. 4 %. page showed a single band for the purified creatinase
3 、肌酸水解酶的提純酶在硫酸銨飽和度為40 80之間完全沉澱，先後經過deae - cellulose離子層析柱、 toyopearlhw - 65疏水層析柱、 sephadexg - 200分子篩層析柱層析，最終使酶提純10倍，最終得率為17 . 4 。Advances on molecule - bolt selective monochlorination and reaction - separation coupling techniques for chlorination reaction in the world were introduced, and the control technology which achieved high selectivity was showed
通過介紹國內外分子篩擇形催化氯化及氯化反應分離耦合工程技術的進展，展示實現有機氯化反應高選擇性的控制技術，為氯化產品工業技術的提升提供參考。Abstract : plant responses to salt stress via a complex mechanism, including sensing and transducing the stress signal, activating the transcription factors and the corresponding metabolizing genes. since the whole mechanism is still unclear, this review emphasize the biochemical events during the plant adaptation to salt stress referring to an index of importance : the homeostasis in cytoplasm, the biosynthesis of osmolytes and the transport of water. most of these biochemical events were elucidated by study of halophyte and salt - sensitive mutations, also many important genes involved were cloned and used to generate stress - tolerance phenotypes in transgenic plants. on the other hand, about the molecular mechanism in signal transduction, the research of arabidopsis mutations and yeast functional complementation provided helpful traces but not full pathway
摘要植物對鹽脅迫的耐受反應是個復雜的過程，在分子水平上它包括對外界鹽信號的感應和傳遞，特異轉錄因子的激活和下游控制生理生化應答的效應基因的表達.在生化應答中，本文著重討論負責維持和重建離子平衡的膜轉運蛋白、滲調劑的生物合成和功能及水分控制.這些生理生化應答最終使得液泡中離子濃度升高和滲調劑在胞質中積累.近年來，通過對各種鹽生植物或鹽敏感突變株的研究，闡明了許多鹽應答的離子轉運途徑、水通道和物種特異的滲調劑代謝途徑，克隆了其相關基因並能在轉基因淡水植物中產生耐鹽表型;另一方面，在擬南芥突變體及利用酵母鹽敏感突變株功能互補篩選得到一些編碼信號傳遞蛋白的基因，這些都有助於闡明植物鹽脅迫應答的分子機制。The total rna was purified from the germ in the liquid by the guanidine isothiocyantehod method, then the total rna digested by dnase that had not rnase was used for rt - pcr. i change the magnesium ion dencity in the pcr system in order to optimize the pcr condition. at the end i selected the magnesium ion density as 1. 25 mm. the production of rt - pcr was inserted directionally into pgem ? z ( ampr ). the pgem ? z ( ampr ) was used to transform e coli jm109. i got a positive clone through culling and identificatin. the dna sequence inserted into pgem ? z ( ampr ) was sequenced and blasted with the cdna sequence of the # - mannanase mature peptide that got from genbank
分取誘導培養液中的菌體，用異硫氰酸胍法提取總rna ，總rna再經無rna酶的dna酶處理後用于rt ? pcr 。在pcr擴增目的基因時，通過優選擴增體系，使鎂離子濃度為1 . 25mm時rt ? pcr可順利地獲得目的基因，並能定向克隆到載體pgem ? 3z （ amp ~ r ）中。用克隆載體轉化宿主大腸桿菌jm109 ，通過篩選獲取陽性克隆子，對陽性克隆子進行酶切與pcr鑒定，並對載體中插入的目的基因進行測序。分享友人