離心純化法 的英文怎麼說

中文拼音 [líxīnchúnhuàfǎ]
離心純化法 英文
centripure process
  • 離 : Ⅰ動詞1 （離開） leave; part from; be away from; separate 2 （背離） go against 3 （缺少） dispens...
  • 純 : 形容詞1 （純凈; 不含雜質） pure; unmixed 2 （純粹; 單純） simple; pure and simple 3 （純熟） skil...
  • 法 : Ⅰ名詞1 （由國家制定或認可的行為規則的總稱） law 2 （方法; 方式） way; method; mode; means 3 （標...
  • 離心 : 1 （不是一條心） be at odds with the community or the leadership2 （離開中心） centrifugal; eccen...
  • 純化 : purification; purifying; depuration; edulcoration; purify
  1. Distillation : thoroughgoing and separated 100 % free of the injurious heavy metal and chemical toxicant of the water to distill most pure water for the health and relieved in always

    採用蒸餾法：將水中所有有害重金屬及化學毒物100 %徹底分離，製造出最晶純的水，使您喝出健康，喝的安心。
  2. The virus was purified by ultracentrifuge. according to the ns gene sequence published by genbank, one primer t - 1 was designed to amplify the cdna by reverse transcript. the other primers nsl - u / ns1 - 1 and ns2 - u / ns2 - 1. hns2 - u were designed to amplify the ns1, ns2 and hns2 gene

    本實驗用接種雞胚的病毒增殖方法獲得了a / chicken / mudanjiang / 0823 / 00 （ h9n2 ）分離株的大量增殖，增殖病毒經差速離心進行純化，經超迷離心濃縮。
  3. In this experiment, radio - immunoassay and hybridization in situ were applied to observe the insulinotropic activities of glp - 1 ( 7 - 36 ) nh2 and reveal the mechanisms underlying this process. methods : rat pancreases were removed from 3 - 5 day - old sprague - dawley rats and dissected into 0. 5mm3 segments and islets were isolated by the collagenase digestion method of wangling et al. thoroughly washed islets and suspended in modified rpmi - 1640 medium supplemented with 10 % fetal bovine serum, and added to 50ml cell culture flasks

    方法：胰島的分離參照王玲等的方法，每次實驗取新生3 - 5天sd大鼠，無菌條件下剖腹取出胰腺，剪切為0 . 5mm ~ 3的組織塊， v型膠原酶消化30min后，離心洗滌，懸浮於完全培養基，接種入50ml培養瓶，於5 co _ 2 、 95空氣條件下培養20h ，轉板純化，接種於96孔培養板培養24h ，按實驗要求進行實驗。
  4. In this paper, the crystal structure of montmorillonite is introduced and the purification methods ( including gravity separation, hydraulic classification, flocculation, centrifugal separation and chemical purification ), the purification principles and purification effectiveness of the methods were summarized

    摘要總結了蒙脫石的各種提純方法及其試驗原理和提純分離效果，主要涉及重力選礦、水力分析、絮凝法、離心分離和化學提純。
  5. Study on the rapid purification of rat islet cells with push - type tube by modified mono - density gradient method

    推進式離心管結合單密度梯度法快速純化大鼠胰島
  6. The crude extraction of dry pea seed, which was obtained through marination, homogenization, filtration, centrifugation and other methods, was precipitated by 50 mmol / l ( final concentration ) mgq2. the pellet was chromatographed on aca ^ gel filtration and deae - cellulose 52 ( de 52 ) anion - exchanger. single eluting peak containing ferritin was obtained finally

    2 、豌豆種子鐵蛋白的純化豌豆種子經浸泡、勻漿、過濾、離心等操作得到豌豆鐵蛋白粗提液，再用終濃度為50mmol / l的mgcl _ 2鹽析等處理后，經aca _ （ 22 ）凝膠過濾柱層析和deae -纖維素52 （ de52 ）陰離子交換柱層析等方法進行純化，得到單一的豌豆鐵蛋白洗脫峰。
  7. The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action, thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained. in this study, we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template. the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons

    本研究設計以c tamrna為模板的反義cdna片段，從c ta基因5 』端第94位到500位核苷酸段設計引物，目的片段407bp ，覆蓋第116和188位兩個aug密碼子，也包含了第一外顯子和第二外顯子間的剪接位點：用常規分子生物學方法構建了反義片段的腺病毒表達載體（ padeasy - 1系統） ；腺病毒載體經hek293細胞包裝產生含反義片段的重組腺病毒，用氯化銫密度梯度離心法獲得純化的高滴度腺病毒；進行體外基因轉移，分別用反義片段真核表達載體轉染p388d1細胞和用重組腺病毒感染hela細胞，觀察導入的c ta基因反義rna抑制細胞內組成型或誘導型c ta基因表達的作用，從而達到調控mhc -類分子表達的目的。
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