離體培養法 的英文怎麼說

中文拼音 [lítǐpéiyǎngfǎ]
離體培養法 英文
in vitro culture method
  • 離 : Ⅰ動詞1 （離開） leave; part from; be away from; separate 2 （背離） go against 3 （缺少） dispens...
  • 體 : 體構詞成分。
  • 培 : 動詞1. （在根基部分堆上土） bank up with earth; earth up 2. （有目的地使成長、壯大） cultivate; foster; train
  • 養 : Ⅰ動詞1 （供養） support; provide for 2 （飼養; 培植） raise; keep; grow 3 （生育） give birth to ...
  • 法 : Ⅰ名詞1 （由國家制定或認可的行為規則的總稱） law 2 （方法; 方式） way; method; mode; means 3 （標...
  1. Thus, in order to investigate the developmental pathways not only involved in the regulation of growth and patterning, but also in the determination of cell lineages and differentiation, we utilized the fluorescent immunohistochemical methods, flow cytometry analysis sorting ( facs ) and molecular methods to investigate the developmental law of mammary gland at the different developmental stages, distribution of the stem cells in mammary gland, the methods of isolation, culture and evaluation for the stem cells, the multipotent abilities in vivo and in vitro, and the efficient cultural system for stem cells enriched in vitro. the results showed below : 1

    我們以小鼠為模型，運用組織化學、免疫熒光組織（細胞）化學、流式細胞儀分選方法（ facs ）以及分子生物學手段，研究了小鼠乳腺的發育規律：小鼠乳腺組織中類乳腺幹細胞：小鼠乳腺細胞的分離、培養以及類乳腺幹細胞的鑒定；小鼠類乳腺幹細胞分化的潛能；小鼠乳腺類腺體體外短期培養富集類乳腺幹細胞體系的優化等。研究結果表明： 1
  2. Eng. ) preparation of media, culture of bacteria, isolation and purification of bacteria, preservation of bacterial strain, gram stain and observation of bacterial strain, biochemical test, growth curve, preparation and analysis of bacterial dna

    中）培養基的制備，菌株的培養，菌株的分離及純化，劃線分離法，及連續稀釋法，菌株的保存，菌株的格蘭氏染色法，菌株生化反應的測試，菌株生長曲線的測定，菌株的染色體dna之制備及分析。
  3. Primary culture of rat preadipocyte were prepared from the epididymal, inguinal and perirenal the fat pads of male normal, healthy, 15 - 20 days sprague - dawley rats. the preadipocyte grew better under the condition of 37, 95 % humidity, 5 % co2, ph 7. 0 - 7. 2, centrifuged at 1000r / min, m199medium, and 10 % fetal bo vine serum, seeded at a density of 4 l04, 5 l04, / cm2. oil red o staining was the special method to distinguish adipocyte from other cells, gimsa and he could determine the stage of the adiopcyte differentiation through the number of lipid drop, size and the position of the nucleolus of the staining fat cell

    經過多次實驗，確定本實驗室大鼠前體脂肪細胞的最佳培養條件是：溫度為37 ，濕度為95 ， co _ 2濃度為5 ， ph值為7 . 0 7 . 2 ，離心力為1000r / min ，培養基為m _ （ 199 ）培養基，胎牛血清濃度為10 ，合適細胞接種密度為4 10 ~ 4 、 5 10 ~ 4個/ cm ~ 2 ，染色結果表明：油紅o染色是鑒定脂肪細胞的特異方法， gimsa和he染色可根據不同區域染色程度、著色差別判斷細胞核的位置及脂滴大小、多少，觀察大鼠前體脂肪細胞分化過程中的形態變化，進而確定脂肪細胞的分化階段。
  4. In this paper, the progress of isolated microspore culture was reviewed : the major factors affecting regeneration ; the methods of reduplication ; the major factors affecting the formation of embryo, for example, experimental material, culture medium, raise condition, a phase of microspore growth

    通過對影響大白菜游離小孢子胚胎發生的主要因素材料基因型、供體母株生長狀態、小孢子發育時期、培養方法、培養條件和影響植株再生頻率的因素以及小孢子植株的加倍方法等進行了綜述。
  5. In terms with the principle of fusarium oxysporiun caused plant disease : bundles were blocked and fusarid acid killing cells was formed by hyphae so that caused water metabolism abnormal and plant wilting. in order to find out effective method of anti - fiisarium oxysporuin, long ya lillium was taken as material with plant tissue culture and genetic transformation techniques in this paper

    針對尖孢鐮刀菌的致病機理：菌絲阻塞維管束引起水分代謝失常和菌絲在植物體內產生毒素（鐮刀菌酸）損害膜結構造成代謝失常，從而導致植物萎焉。本實驗以龍牙百合為研究對象，應用細胞工程中的離體培養方法並結合轉基因技術，以期找到抗尖孢鐮刀菌的有效途徑。
  6. By the compounds of submandibular gland cells and collagen sponges. we investigate the optimal cell denisity of tissue engineered compound of submandibular gland cells and collagen sponges, the cellular compatibility of tissue engineered compound of submandibular gland cells on the collagen sponges with different porosity and the influence of epidermal growth factor on the adherence of submandibular gland cell to collagen sponge. our studies can primary provide theoretical ground work to form the model in vitro of tissue engineering smg

    在本研究中，以初步探討體外頜下腺細胞與膠原海綿支架相互作用為目的，採用體外分離培養sd大鼠頜下腺細胞，然後接種于膠原海綿支架上體外復合培養的方法；從不同接種細胞濃度對細胞一支架復合物影響，同一接種細胞濃度在不同孔隙率的支架上黏附、增殖的情況及表皮生長因子（ egf ）對頜下腺細胞的促增殖作用，促細胞在支架上黏附等三方面入手，初步研究了頜下腺細胞與膠原海綿相互作用的影響因素，為進一步在體外及體內構建較為理想的組織工程化頜下腺提供理論參數和實驗依據。
  7. To identify the key factors affecting primmorph formation and to optimise the technique for developing an in vitro primmorph culture system, a chinese sponge, stylotella agminata ( ridley ), collected from the south china sea, were investigated

    確定了海綿細胞離體培養的細胞生長的檢測方法。論文以南海攜突針海綿（ stylotellaagminata （ ridley ） ）為研究對象，建立了海綿混合細胞團（ mcp - primmorph ）形成及培養的基本方法。
  8. The time required for primmorph formation, primmorph size distribution, and the proliferating capability were microscopically documented. for a successful formation of primmorphs, it is mandatory to use healthy sponge tissues and a minimum inoculate cell density is required. culture temperature will need to be adapted to the environmental temperature where the sponges are collected

    對mcp - primmorph的生長，增殖和代謝特徵進行5天連續培養考察，發現海綿細胞團離體培養方法的建立及其成團增殖和代謝規律研究了mcp一prllnmorph培養中存在有絲分裂細胞，但增殖細胞比例僅為巧%左右，且上述培養基對增殖無顯著影響。
  9. During the culture of the porcine ear skin fibroblasts, the successful rate was increased by selecting culture methods, contrasting donor age and improving culture condition. the rate of living cells in the method of combining trypsin cold treatment with trypsin heat treatment was much higher than that in the method of trypsin heat treatment. the results showed that the method of combining trypsin cold treatment with trypsin heat treatment had less adverse effects on cells than the method of trypsin heat treatment

    在培養豬耳皮膚成纖維細胞時從篩選細胞分離方法、比較供體年齡和改善培養條件這三方面著手提高培養的成功率。實驗中發現胰蛋白酶冷熱處理結合法培養的細胞存活率明顯高於胰蛋白酶熱處理法，說明使用胰蛋白酶冷熱處理結合法對細胞的不利影響較少，細胞產量較高。
  10. Improvement on isolated and cultivated method of borrelia burgdorferi

    萊姆病伯氏疏螺旋體分離培養方法的改良
  11. High performance ion exchange chromatography was applied in studying qualitatively and quantitatively of bacteria, which was shown as follows : firstly, physio - biochemical characteristics of bacteria was investigated by ion exchange chromatography. for the first time spores and nutrient of bacillus pumilus had been separated successfully by chromatography. chromatographial behaviors of bacteria at different cultivating environment and different growth phase were also studied

    本文利用高效液相離子交換色譜系統研究細菌學，探討了該方法在細菌定性、定量方面的應用，主要包括三個方面：首先，利用離子交換色譜系統表徵細菌生理、生態方面的變化，首次成功地在色譜上區分了短小芽孢桿菌的芽孢及營養體；考察了不同的培養環境對細菌色譜行為的影響及不同生長階段的細菌的色譜行為。
  12. This paper deals with the methods for in vitro conservation of gesneriads. the another purpose of this experiment is to search and demonstrate the high regenerative capacity of leaf tissue of gesneriads

    本實驗主要以刺齒唇柱苣苔和煙葉唇柱苣苔為主要實驗材料，運用組織培養的方法，對苦苣苔植物的離體保存、快速繁殖過程進行探討。
  13. In order to investigate the tolerance of ectomycorrhizal fungi to heavy metals in vitro, three culture methods, namely liquid culture without agitation, liquid culture with agitation and solid agar culture, were investigated to determine which method would give the best combination of fungal biomass and ec50. the results indicated that liquid medium without agitation was the best culture method

    為研究外生菌根真菌本身對重金屬污染的耐性，比較了液體靜置、液體搖床和瓊脂固體培養這三種常用的菌絲體的純培養方法，以真菌生物量大小和分離難易程度為主要指標，篩選出液體靜置方法為最優方法。
  14. The total rna was purified from the germ in the liquid by the guanidine isothiocyantehod method, then the total rna digested by dnase that had not rnase was used for rt - pcr. i change the magnesium ion dencity in the pcr system in order to optimize the pcr condition. at the end i selected the magnesium ion density as 1. 25 mm. the production of rt - pcr was inserted directionally into pgem ? z ( ampr ). the pgem ? z ( ampr ) was used to transform e coli jm109. i got a positive clone through culling and identificatin. the dna sequence inserted into pgem ? z ( ampr ) was sequenced and blasted with the cdna sequence of the # - mannanase mature peptide that got from genbank

    分取誘導培養液中的菌體，用異硫氰酸胍法提取總rna ，總rna再經無rna酶的dna酶處理後用于rt ? pcr 。在pcr擴增目的基因時，通過優選擴增體系，使鎂離子濃度為1 . 25mm時rt ? pcr可順利地獲得目的基因，並能定向克隆到載體pgem ? 3z （ amp ~ r ）中。用克隆載體轉化宿主大腸桿菌jm109 ，通過篩選獲取陽性克隆子，對陽性克隆子進行酶切與pcr鑒定，並對載體中插入的目的基因進行測序。
  15. Shows how fibroblasts from human dermis are isolated by using enzymes and then cultured in nutrient medium

    示?如何使用酵素分離人類皮膚的纖維組織母細胞在營養培養基培養，如何計算數目及液體氮冷凍儲存法。
  16. All the 4 extractions showed obvious pharmacological effects by decreasing the area of myocardial infraction of the model rats, increasing the amount of blood stream of the isolated guinea - pig hearts and reducing the beating rate and amplitude of cultured myocardial sheets of mice

    方法：採用冠狀動脈結扎致大鼠急性心肌缺血模型，觀察革葉獼猴桃果實70 %乙醇提取物、正己烷部位、氯仿部位及正丁醇部位對急性心肌梗塞范圍的影響，同時觀察上述部位對豚鼠離體心臟冠脈流量及小鼠胚胎心肌培養細胞功能的影響。
  17. The paper overviews the formation and characteristics of the technique of rapid propagation of free virus in plant tissue culture, and its application in flower, wood, fruit tree, vegetable. . etc., and introduces the main technical link to produce the seedling, including the function and choice of media, the principle and need of donor plants, tame method and transplant request of plant, productive plan of seedling and budge means of cost

    摘要綜述了植物離體快速繁殖技術和脫毒技術的形成、特點及其在花卉、林木、果樹、蔬菜等方面的應用，闡述了利用快繁與脫毒技術生產種苗的主要技術環節，包括培養基的作用和選配要點、外植體選取的原則和快繁與脫毒的不同要求、試管苗馴化的方法與移栽要求、種苗生產計劃的制定與成本預算方法。
  18. The main contents and results of the study are as follows : 1. the separation, culture and purification of black bear fibroblast cell for the donor of nuclear transfer the black bear fibroblast cell can be obtained from ear skin and neck skin of the black bear by issue culture method. the cells were cultured in dmem and rpmi1640 mediums containing 10 % fetal bovine serum ( fbs ), respectively

    主要內容和結果如下： （一）黑熊成纖維細胞的分離培養和純化從黑熊耳緣皮膚和頸部皮膚取材，採用組織塊培養法分離培養黑熊皮膚成纖維細胞，在dmem和rpmi1640兩種培養基中分別添加10的胎牛血清，均可滿足黑熊皮膚成纖維細胞的體外生長和傳代。
  19. These results are consistent with the results from gross and salzstein and so on. osteoblast is the seeded cell of bone tissue engineering. now, in order to research the response of the osteoblast when it is cultured with mechanical stimulating, the paper consider the osteoblast as an homogeneous, isotropic, incompressible viscoelastic medium

    由於成骨細胞是骨組織工程的種子細胞，為研究其在機械刺激離體培養下的受力情況，論文將成骨細胞看作是一種均勻、各向同性、不可壓縮粘彈性體，然後應用數值方法將物理關系中的遺傳積分展開，從而導出物理關系的矩陣形式。
  20. Image processing applying in analysis of motion features of cultured cardiac myocyte in rat

    圖像處理方法用於離體培養乳鼠心肌細胞動態特徵分析
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