離體活化分析 的英文怎麼說

中文拼音 [lítǐhuóhuàfēnxī]
離體活化分析 英文
in vitro activation analysis
  • 離 : Ⅰ動詞1 （離開） leave; part from; be away from; separate 2 （背離） go against 3 （缺少） dispens...
  • 體 : 體構詞成分。
  • 活 : Ⅰ動詞1 （生存; 有生命） live 2 [書面語]（救活） save （the life of a person）:活人無算 （of a goo...
  • 分 : 分Ⅰ名詞1. （成分） component 2. （職責和權利的限度） what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] （料想） judge
  • 析 : Ⅰ動詞1. （分開; 散開） divide; separate 2. （分析） analyse; dissect; resolve Ⅱ名詞（姓氏） a surname
  • 活化 : [化學] activation radioactivation; activate活化處理 activating treatment; 活化分析 [核物理] activ...
  1. Through the analyzing the macro - structure to micro - structure, the author considers that retarding mechanism of citric acid is that citric acid and ca in the gypsum form the complexant ion, and hinder the crystallization center of dihydrate crystal bigger ; that of sodium tripolyphosphate is that it combined with ca, hinders the dissolve of hemihydrate. formation and growth of crystallization center ; that of bone glue is the glue - protection and chemical absorption action of active group, which also retards the formation and growth of crystallization center

    通過宏觀到微觀的分析，筆者認為，檸檬酸的緩凝作用的原因主要在於檸檬酸與鈣形成絡合離子，影響了二水石膏晶體的晶核長大過程；多聚磷酸鈉與鈣形成某種復鹽，對于石膏晶體的溶解、成核和長大過程均有強烈的阻礙影響；骨膠則在於膠體對半水石膏的包裹和活性基團的化學吸附，使二水石膏晶體的成核和長大困難。
  2. Strain bl21, and gene expression was induced by iptg. the target proteins were directed into the periplasmic space by the staphylococcal protein a signal sequence preceding the rgd - hirudin gene. using ion exchange chromatography and gel filtration chromatography, the chimera proteins were purified, and both of them showed a single band in tricine - sds - page. the results of activity analysis suggested that these two chimera proteins not only have antithrombin activities, but gain platelet aggregation inhibitory activities as well

    通過離子交換層析和凝膠過濾層分別對兩種嵌合體蛋白進行純化，純化產物在tricine - sds - page中都顯示為單一條帶。活性分析結果表明兩種嵌合體蛋白在保留水蛭素抗凝血酶活力的同時，還呈現抗血小板聚集活性。
  3. Recovery of this photoinhibition is a complicate but orderly course, including degradation of photodamaged d1, synthesis and assembly of new one, etc. using lincomycin to block the replacement of new synthetic dl protein into photodamaged one, the spinach leaves was exposed to highlight, giving rise to photoinhibition before the thylakiod membranes were isolated

    解除光抑制后， ps活性恢復是一個復雜而有序的過程，需要d1蛋白降解、新合成d1蛋白和重組裝ps等。實驗首先進行菠菜葉片光抑制處理，加入林可黴素阻斷葉綠體蛋白質合成，利用尿素sds變性電泳分離類囊體膜蛋白，藉助d1蛋白抗體westen免疫印跡、磷酸化蛋白快速檢測方法分析d1蛋白存在形式，並進行定量分析。
  4. Based on the extensive studies of subtilisin - like protease ( prl ) of metarhizium anisopliae, extracellullar serine protease is suggested to be a key enzyme involved in the fimgal penetration to invertebrates. the investigation of serine protease in the nematode infected by owvtl may help to understand the mechanism of nematophagous fimgi as biological control agents. a 3l kda serine protease was isolated and purified from the liquid culture of h rhossiliensis owvtl challenged with nematode panagrellus redivivus

    本研究利用線蟲誘導下owvt - 1菌株液體發酵，通過粗分級分離、離子交換層析和凝膠過濾層析分離提純了一個分子量為31kda的絲氨酸蛋白酶，生物學測定表明其對大豆胞囊線蟲二齡幼蟲具有致死作用，同時測定了該酶理化特性，酶活力在75附近酶活力最高，隨著ph的增加酶的穩定性升高，與膽堿酯酶具有相似的ph曲線，對特異性底物aape （ suc - ala - ala - pro - glu - pna ）具有作用， ssi和ci - 2抑制該酶的活性。
  5. Recently, study on solvent polymeric membrane ( spm ) anion selective electrodes based on metal complexes as neutral carrier and exhibiting anti - hofmeister selectivity pattern is an important project in the fields of electrochemistry and electroanalytical chemistry

    近年來，以金屬配合物作為中性載體且呈現anti - hofmeister行為的陰離子選擇性電極是電化學和電分析領域中公認的活躍研究課題。
  6. The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins

    將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。
  7. This article confirmed the grafting efficiency of starch grafted to acrylic acid and the structure of starch and its grafted matter through the separation, purification and analysis tests to the grafted copolymer and the grafted forked - chain matter produced from the process of this reaction ; and deduced the formula of the velocity of such reaction mentioned above with a calculated result of the apparent activation energy in this reaction by the curve showing the relationship among the consistency of starch, monomer and initiator and the velocity of the grafting copolymerization

    摘要對澱粉接枝丙烯酸所得接枝共聚物及接枝側鏈物進行分離提純和分析測試，確定了其接枝效率並表徵了澱粉及其接枝物的形態結構；通過分別測定接枝共聚反應速率與澱粉、單體、引發劑濃度的關系曲線，確定了該接枝共聚反應的速率關系式；推算出了反應的表觀活化能。
  8. A novel aqueous two - phase system can be formed by the mixtures of a polymer and cationicanionic surfactants. such a system can be used as a partitioning system of proteins. in this work, we investigated the formation, phase behavior and protein partitioning in aqueous two - phase systems formed by dodecyltriethylammonium bromide / sodium dodecylsulfate / peg and dodecyltriethylammonium bromide / sodium dodecylsulfate / dextran. the ligands with affinity were attached to the polymers and the affinity partitioning of proteins was investigated. it was shown that the surfactants and polymers are enriched in different phases of aqueous two - phase systems. phase separation are promoted by increasing temperature and adding inorganic salts. different proteins are partitioned in different phases. the selectivity of protein partitioning is increased by adding ligands with affinity

    報道了由正負離子表面活性劑與高聚物混合溶液形成的一種可用於蛋白質的分離及分析的新型雙水相萃取體系.研究了正負離子表面活性劑（溴化十二烷基三乙銨/十二烷基硫酸鈉）分別與葡聚糖和聚乙二醇混合雙水相體系的形成規律、相行為及牛血清蛋白和溶菌酶在雙水相體系中的分配.通過在高聚物分子中接上親和配基，研究蛋白質在雙水相體系中的親和分配.結果表明，在該體系中，表面活性劑與高聚物分別富集於不同相中.升高溫度及加入無機鹽均可促進雙水相體系的形成，不同蛋白質可分配于不同的相中.親和配基的引入極大地增強了蛋白質分配的選擇性
  9. Using biomechanics methods, through the study of the influence of different injection and maintain period on the expansion efficacy and shrinkage rate of skin, a rapid expansion method with relatively big expansion area is proposed ; biomechanics analysis in vivo is been made on the expanded skin ; comparing the biomechanics property in vitro of the rapid expansion and conventional expansion with different maintaining times, the parameters such as strength, stress - strain relationship, stress relaxation and creep which reflect the characteristic of the expanded skin have been obtained ; making the research of the expanded skin ' s biomechanics changes at different stage after grafting, the long - term and short term diversified regular has been obtained ; making experimental study and discussing the changes in the microcirculation and vessel structure of exceedingly expanded shin, the relationship between survival length of flap and the changes has been gotten

    本課題旨在用生物力學的方法通過動物實驗來研究不同注水擴張期和維持擴張期對皮膚擴張量和皮膚回縮的影響，探討有效獲取較大的擴張皮膚面積的快速擴張方法；對擴張皮膚進行在體生物力學特性分析；比較了快速擴張和常規擴張在不同維持期的皮膚離體生物力學特性，得到離體生物力學各參數，如強度、應力?應變、應力鬆弛、蠕變等；為全面了解擴張皮膚在移植后的近期和遠期的變化規律，做了擴張皮膚移植后不同階段的生物力學變化的實驗研究；探討了不同擴張量時皮膚的微循環變化和血管結構變化與皮瓣存活的關系。
  10. Secondly, among the single mothers, those who have the experience of divorce tend to hold that their own efforts are not useful for the improvement of marriage relations, and to believe that if crises occur in their marriages, divorce is the inescapable fate and it is the spouses " behavior that leads to the failure of marriage. thirdly, those who have lost their husbands will more idealize their marriages, and blame much more of themselves when there are some crises in their marriages. fourthly, the factors such as personality, age, years of education, years of being single will influence the perception of the underlying causes of satisfaction or dissatisfaction in marriage, and meanwhile the attribution in marriage is correlated with the swb ( subjective well - being ) and satisfaction of life

    根據調查的結果，我們發現： （ 1 ）單身母親生活負擔沉重、健康狀況不佳、心理壓力大、對生活質量評價低、主觀幸福感差，是弱勢群體中的弱者； （ 2 ）單身母親群體中，有過離異經歷的婦女更傾向于認為自身的努力無助於改善婚姻關系，如果婚姻出現危機，破裂將是難以避免的，並將失敗的責任更多地推卸給外部因素； （ 3 ）喪偶組婦女在分析婚姻成敗的原因時，表現出將婚姻生活理想化的傾向，並且在婚姻出現危機時，比其他婦女更傾向于將責任歸咎為自己，而產生更多的自責情緒； （ 4 ）性格特徵、年齡、受教育程度、單身時間的長短等因素都會影響個人對婚姻成敗原因的判斷，同時，對婚姻成敗的歸因與主觀幸福感、生活滿意度都有一定的相關。
  11. These landslides go with fluidization peculiarity different degree in course of their motion, and their speed and distance increase several times because of the fiuidization, thereby the disaster area greatly broaden. on the base of above, the paper studies systematically the fluidization of large high - speed rockslides in all its aspects : analyzed the aerodynamic effect in the short - range on the base of the wind turmel experiment, studied the effect of falling into pieces when landslides collides mountain ; studied the accelerating effect through high - speed collide experiment of landslides model ; and the paper reappear the overall process including set - out, collide in the short range, debris flow in the long - range of touzhai large landslide via distinct element method ( dem ), studied the flow law and equation of different type of debris flow resulted from large landslide. as a sample to apply, the paper analyzed fluidization mechanism of touzhai landslide

    基於這一點，本文對大型高速巖質滑坡的流體化現象進行了全面而系統的研究：通過風洞模型實驗，研究了大型高速滑坡凌空飛行階段的空氣動力學效應，研究了飛行中滑坡巖體在與途中不動山體發塵碰撞時的解體破碎效應，通過滑坡巖體高速碰撞模型實驗研究了滑坡巖體解體破碎後部分巖體的加速運動效應，並應用離散單元法再現了雲南頭寨溝大型滑坡啟動高速、近程碰撞、遠程碎屑流運動的全過程，研究了巖體解體破碎后形成不同類型碎屑流的運動規律及運動方程，並對頭寨滑坡流體化運動機理進行了實例分析，取得了以下主要結論和成果： （ 1 ）對于大型高速滑坡，大多在啟程活動階段已獲得了高速，這使得這類滑坡在其離開剪出口之後以凌空飛行的形式向前運動，在其飛行過程中，滑坡巖體與空氣強烈作用，並產生空氣動力學效應，滑坡巖體在因碰撞而產生的解體破碎效應及部分巖體的加速運動效應的作用下，在遠程活動階段，以高速碎屑流的形式向前運動。
  12. Then combined with the two main mechanisms it is putting forward the idea of compound - mechanism antimicrobial. as a result, there come the two types of nano - inorganic antimicrobials : ( 1 ) ion - sterilizing antimicrobial which utilized the sterilization function of re and used the powder ( active sio2 ) as carrier. ( 2 ) compound - mechanism antimicrobial which has both the sterilization function of re and the light - catalyzing sterilization function of nano - tio2 whose wavelength of light - absorption has einstein shift because of the mix - into of re

    首先對市場上主要的抗菌劑產品進行歸類，對其殺菌機理進行對比分析，結合離子殺菌與光催化殺菌，提出了復合機理抗菌劑的思想，研製出：以稀土為殺菌主體，活性sio _ 2為載體的離子型抗菌劑；以稀土為離子殺菌部分，納米tio _ 2為載體，且在稀土離子的摻入下，納米tio _ 2的光吸收波長發生紅移，進入可見光區而提高光催化能力的復合機理抗菌劑。
  13. Sectionii : to research and establish a kind of high efficient purification model of recombinant proteins produced in escherichia coli as inclusion bodies. to raise separate efficiency and biological active efficiency is especially important in the purification model of recombinant proteins. in accordance with these questions, we raise inclusion bodies purity before the purification as far as possible, and select the suitable chromatogram technology

    研究並建立一種包涵體中高效分離純化目的蛋白的優化模式重組蛋白分離純化工藝中分離效率的提高、生物活性效率的提高及工藝的穩定性是尤其重要的，針對這些問題，我們在層析純化前盡山西醫科大學生物化學與分子生物學2003屆碩士學位論文可能地提高包涵體純度，並選擇合適層析方法的同時合理的組合色譜分離單元。
  14. In addition, whether and how the bl receptor photl regulate ca2 + - channels in the pm has not been investigated. in present study we aimed to identify ca2 + channels involved in bl signaling cascade. the patch clamp technique was applied to record the channels on the hypocotyl protoplasma of wild type and photoreceptor mutant of arabidopsis thaliana

    採用膜片鉗技術在擬南芥下胚軸原生質體質膜上記錄並鑒定到一種超極化激活的鈣離子通透性通道，對其特性分析發現photl - 5突變體與野生型擬南芥的質膜鈣離子通透性通道電流有顯著差異，該差異可能是二者對胞外鈣表現不同反應的原因。
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