電泳性質 的英文怎麼說
中文拼音 [diànyǒngxìngzhí]
電泳性質
英文
electrophoretic property-
Our previous studies showed existence of apoplast cam in the plant cell and cam had many extracellular functions. so it supposed cam may be one of important extracellular polypeptides and trigger the intracellular signal transduction by binding the receptor. in this study, radiolabelled ligand is used to investigate the binding characteristic of cam and a. thaliana protoplasts. and chemical crosslinking is employed to explore binding proteins in the membrane. at first, ( 35 ) ~ s - cam was produced using ( 35 ) ~ s - labeled amino acid mixture in e. coli. sds - page and autoradiograph indicated high - purified, high - specific radioactivity ( 35 ) ~ s - cam was obtained. electrophoresis of ( 35 ) ~ s - cam is the same as that of unlabeled cam with ca ~ ( 2 + ) or egta ; a quatitive of protoplasts was prepared by enzymolysis
首先，用~ （ 35 ） s標記的氨基酸混合物喂養工程菌成功地制備了~ （ 35 ） s標記的擬南芥鈣調素（ ~ （ 35 ） s - cam ） ， ~ （ 35 ） s - cam純度高、放射活度高、 ca ~ （ 2 + ）與egta存在時的電泳行為與未標記cam相同，可作為一種高靈敏性的探針用於進行受體學分析實驗；用擬南芥種子誘導愈傷，通過酶解制備了大量原生質體。That is the premise of the bg / ha electrophoresis codeposition. the laws of the electrophoresis deposition of the bg and ha partic les were found by the study on each of their deposition under the different conditions. the electrophoresis codeposition of the bg and ha particles had been studied and the bg / ha graded coating, which is compact in the bottom layer and porous near the surface layer, had been prepared on the surface of the dental implant after the low temperature heat treatment ( about 740 ) and fast firing ( 50 - 80 / min, heat preservation time was 5 - 8min. )
以bg微粉和ha微粉作為塗層原料，通過研究bg和ha微粉在非水介質中的分散情況和帶電特性，選擇冰醋酸為介質，使分散在其中的bg顆粒和ha顆粒表面均帶上正電荷，為電泳共沉積提供前提條件；通過對不同條件下bg 、 ha各自電泳沉積的研究，探索出了兩者電泳沉積的規律；通過對bg和ha在冰醋酸中電泳共沉積以及后續低溫（ 740左右）快燒（ 50 ? 80 min ，保溫5 ? 8min ）熱處理的研究，在鈦合金牙根種植體基體上成功制備出了底層緻密而表層多孔的bg ha梯度塗層。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。Ampholytes are electrophoresed through the gel prior to sample addition.
在加入樣品前，兩性電解質先通過凝膠電泳。The substance with antibacteria action obtained from forest frog is made up of alanine, aminoacetic acid, leucine, isoleucine, proline, aminoglutaric acid, threonine, serine, lysine. the substance with antibacteria action is a kind of poly peptide with a micromolecul
純化的林蛙皮膚抗菌活性物質經尿素? sds ? page電泳分析，表現為一條帶，分子量約為6 . 28kda 。Recovery of this photoinhibition is a complicate but orderly course, including degradation of photodamaged d1, synthesis and assembly of new one, etc. using lincomycin to block the replacement of new synthetic dl protein into photodamaged one, the spinach leaves was exposed to highlight, giving rise to photoinhibition before the thylakiod membranes were isolated
解除光抑制后， ps活性恢復是一個復雜而有序的過程，需要d1蛋白降解、新合成d1蛋白和重組裝ps等。實驗首先進行菠菜葉片光抑制處理，加入林可黴素阻斷葉綠體蛋白質合成，利用尿素sds變性電泳分離類囊體膜蛋白，藉助d1蛋白抗體westen免疫印跡、磷酸化蛋白快速檢測方法分析d1蛋白存在形式，並進行定量分析。The peroxidase activity of variant t220x was obviously higher than acceptor lu22 at seedling stage. the result of peroxidase electrophoresis indicated that there were difference both in the depth and in the amount of zymogram between lu22 and t220x. so it was concluded that the variety of peroxidase was the result of change of heriditary substance
小麥幼苗期過氧化物酶活性的測定顯示，變異體t220x的酶活性高於受體魯22 ；過氧化物酶電泳顯示， t220x和魯22過氧化物酶在酶帶深淺和數目方面都存在差異，說明外源遺傳物質已影響到過氧化物酶的變化。However according to the view of acoustics, click includes mixed frequencies, so further studies are necessary to investigate the physiological significance of amygdaloid modulating effect on the ascending auditory information at the cortical level. in this paper we firstly observe the characteristics of the acoustic response of neurons in a - i evoked by pure tone ; then investigate the influence of la stimulation on the acoustic response of these neurons and the physiological significance of such influence ; revea l the neural pathway mediating this effect with the neurohistological method ; and study the neurotransmitter and its receptor participating in this effect
本文採用電生理學方法考察大鼠皮層a區神經元純音反應特徵，觀察杏仁外側核（ la ）對a區神經元聲反應的影響，運用神經組織學方法揭示介導這一影響的神經環路，採用多管微電極記錄結合微電泳技術的方法研究參與這種影響的神經遞質及受體，進一步探討了這種調制性影響的生理學意義。The positive colonies that grew on the ampicillin ( amp ) plate ( lb agar medium contaning 100 g / ml amp ) were screened and identified. sds - page and western blot analysis were performed to study the expression profiles of target gene cein in e. coli
從轉化的平板中篩選出陽性重組子，進行不同iptg濃度和不同誘導時間的表達研究，並利用sds - page電泳和westernblotting蛋白質印跡技術對外源基因cei _ （ 12 ）在大腸桿菌eAfter the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s
通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。Evaluating genotoxicity of water quality in lanzhou section of the yellow river using micronucleus test and comet assay
利用微核試驗和彗星電泳試驗評價黃河蘭州段水質致遺傳毒性作用Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry
三、 gst一hbrp重組蛋白的純化1 .超聲破碎細胞，離心，上清和沉澱進行sds一page電泳分析2 .樣品處理提取包涵體，變性后，加人用pbs平衡過的以utal腸onesepb抓脫4b ，室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據毛山lel ，決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積，用預冷的1xpbs清洗cldtathionese戶， se4b ，得到50 %的基質Purification and characterization of phytase from a. niger an 01001 a. niger an01001 was inoculated on solid media and cultivated at 30 for 5 days. proteins were extracted from solid - state fermentation with 50mm acetate buffer ( ph5. 5 ). the molecule weight of the phytase protein was determined as about 78kd by sds - page. the purification procedures include ammonium sulfate precipitation, deae - cellulose ion - exchange chromatography, gel electrophoresis and electroelution
3 .植酸酶的分離純化及其性質研究黑麴黴ano1001經固體發酵，用緩沖液抽提后，經硫酸按沉澱， deae一纖維素離子交換層析，聚丙烯酞胺凝膠電泳和電洗脫等純化步驟獲得的植酸酶，用sds一page檢測為一條均一譜帶，其分子量約為78kd 。Test method for characterization of proteins by electrophoretic mobility
電泳遷移率法測定蛋白質特性的測試方法Based on the infrared anomalous dispersion of compact alumina film, the character of alumina sols and the processing for fabricating compact alumina film were studied by using sol - gel method, combining dip - dropping, spin - coating method and electrophoretic deposition techniques
本文基於緻密氧化鋁膜的紅外反常色散特性，結合溶膠-凝膠技術，採用浸漬提拉法、旋覆法和電泳沉積工藝，對氧化鋁溶膠的性質和緻密氧化鋁膜的制備工藝進行了研究。This understand of stored nitrogen compounds restricted seriously the progress in the investigation of vegetative storage proteins. in the dissertation, we studied more extensively the cytology, biochemical properties and biological roles of vegetative storage proteins in swietenia macrophylla and in hevea brasiliensis by light - and electron microscopy, sds - page, page, immuno - blotting, indirect immunohistochemical localization and colloidal gold labelling and cdna clone techniques
採用光鏡和電鏡技術、 page 、 sds - page和免疫印跡技術、電泳凝膠過碘酸? schiff試劑染色、間接免疫熒光和電鏡免疫細胞化學定位技術以及cdna克隆技術，較深入地研究了大葉桃花心木和巴西橡膠樹的營養貯藏蛋白質的細胞學、生物化學性質和生物學功能。An antifungal protein, named as b16, was purified from the supernatant of the fermentation broth of strain 041381 by 80 % saturation ammonium sulfate, desalt and gel filtration on sephadex g - 75, which with molecular weight at about 30 - 40 kd on the basis of sds - page
菌株041381發酵上清液經70飽和度硫酸銨沉澱，透析脫鹽， sephadexg - 75凝膠過濾層柱，得到活性物質b16 。以sds - page膠為基礎進行電泳分析， b16的分子量為30 - 40kd 。( 2 ) effects on mouse spleen of so2 challenge : we found significant apoptotic changes of mouse spleen through tem observation and dna electrophoresis analysis and flow cytometric analysis. we found condensed, marginating, half - moon like apoptotic lymphocytes both in white pulp and red pulp ; we found significant dna degradation with dna ladders from the dna electrophoresis analysis in the 168 mg / m3 so2 treated group ; we also found marked increase of apoptotic rate between 168 mg / m3 so2 treated group and control group from the flow cytometric analysis
（ 2 ）二氧化硫吸入可引起小鼠脾臟細胞出現明顯的凋亡改變，紅髓區和白髓區淋巴細胞出現核固縮，染色質凝聚、邊集； dna凝膠電泳分析發現168mg m ~ 3二氧化硫染毒組出現典型的dna梯形條帶；流式細胞分析也發現高劑量染毒組的小鼠脾細胞凋亡率增加，並且與對照組相比有顯著性差異， p 0 . 05 。Using three male sterile lines of radish, the activicy of three isozymes were studied during their development of vegetable and reproductive stages
摘要以蘿卜細胞質雄性不育系和保持系為試材，採用聚丙烯酰胺凝膠垂直板電泳方法對花期葉進行過氧化物酶同工酶、澱粉酶同工酶進行分析。In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ), and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee. the plasmid pfb 1 - cee was transformed into dh1 obac competent e. coli cells. high molecular weight dna was prepared from the overnight cultures from the selected e. coli colonies, which was recombinant baculovirus shuttle vector containing hcv structural gene, named bac - cee
本實驗用pcr擴增hcv結構區基因，克隆到桿狀病毒表達載體pfastbacl中，構建成重組轉座載體pfb1 - cee ，轉化dh10bac大腸桿菌感受態細胞，篩選陽性菌落，抽提大分子質粒dna ，獲得含hcv結構區基因的重組桿狀病毒穿梭載體bac - cee ，脂質體介導轉染sf9昆蟲細胞，出現細胞病變后，收集含有重組桿狀病毒顆粒的培養上消，重新感染sf9細胞，收集sf9細胞，進行12 . 5 sds -聚丙烯酰胺凝膠電泳，可見表達的蛋白條帶。分享友人