電泳液 的英文怎麼說

中文拼音 [diànyǒngyè]
電泳液 英文
（reagent i） genmed
  • 電 : Ⅰ名詞1 （有電荷存在和電荷變化的現象） electricity 2 （電報） telegram; cable Ⅱ動詞1 （觸電） give...
  • 泳 : 動詞（游泳） swim
  • 液 : 名詞（液體） liquid; fluid; juice
  1. This system contains : parts load, pre - degrease, degrease, spray cleaning, spray surface adjustment, spraying phosphating, rinse with d - ionized water, electro - deposition, ultra - filter for post rinse, oven curing, cooling and unload

    由上件，預脫脂，脫脂，噴洗，噴淋表面調解，噴淋磷化，去離子水噴洗，電泳塗裝，超濾液噴洗，漆膜固化，自然冷卻，下件工序組成。
  2. The supernatants were dialyzed against 50 mm phosphate buffer ( pb ) ( ph 6. 5 ), and passed through a blue - sepharose 6b column ( 3x30 cm )

    Iptg誘導表達目的蛋白，上清液經sds一page電泳后看到約32kd的目的條帶。
  3. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  4. 二 、 effects of calcium concentration on the membrane - bound gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation. the membrane - bound gq a was extracted from the retina of the macrobrachium rosenbergi with protein extract and was electophoresised by sds - page. the percent of the membrane - bound gq a was analyzed by tanon gis gel image disposal system

    二、鈣離子濃度對光暗適應羅氏沼蝦感光細胞膜結合gq蛋白亞基的影響用蛋白質提取液提取膜結合gq蛋白亞基並sds - page電泳，利用tanongis凝膠圖像處理系統分析其含量。
  5. In our experiment, after light and dark adaptation, the retina of the macrobrachium rosenbergi was respective incubated in high calcium solution, physiological solution and low calcium solution. we studied the effect of calcium concentration on the content and subcellular localization of gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation by sds - page technology and imunoelectron microscopy technology. our study results indicated : 一 、 effects of calcium concentration on the soluble gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation

    而鈣離子對gq蛋白亞基活性有無影響還未見報道。我們以光適應和暗適應條件下的羅氏沼蝦復眼視網膜為材料，分別用高鈣溶液、生理溶液、低鈣溶液孵育后，通過sds ? page電泳技術及免疫膠體金電鏡技術，研究鈣離子濃度對光暗適應時羅氏沼蝦感光細胞gq蛋白亞基含量的影響及亞基亞細胞定位的影響。
  6. Ip3 - ip3 receptor ( ip3r ) interaction mediates the release of ca2 + from the endoplasmic reticulum in response to many different extracellular stimulus. for higher plants, however, though it is now generally accepted that ip3 participates in signal transduction in many important cellular processes, only limited evidence is available for the presence and properties of the ip3r - like protein so far. here, using the immunological methods with an antibody raised against a mammalian inositol 1, 4, 5 - triphophate receptor ( type 1 ), we found that, 1 ) the antibody across - reacted the proteins with about 200kd in microsomes from oryza sativa and about 200kd from arabidopsis thaliana respectively

    本實驗用sds - page電泳和免疫印跡的方法，用哺乳動物大鼠三磷酸肌醇受體的多肽做抗體對類三磷酸肌醇受體蛋白鑒定，結果表明：抗體與水稻和擬南芥微粒體蛋白分子量大約為200kd的蛋白交叉反應，同時還發現在水稻微粒體蛋白62kd和擬南芥微粒體蛋白45kd處有交叉反應的蛋白條帶存在，表明在植物中有類三磷酸肌醇受體蛋白的存在；用免疫膠體金方法，發現類三磷酸肌醇受體蛋白主要分佈於液泡膜和細胞質膜上。
  7. It manufactures various high performance industrial coatings, including coil coatings, wood coatings, general industrial coatings liquid coatings, powder coatings, electrocoat, auto refinish coatings, mirror coatings, etc

    公司生產各種高性能的工業塗料，包括卷鋼塗料、木器塗料、一般工業漆塗料（農用工業機械液體塗料、粉末塗料、電泳塗料） 、汽車修補漆、鏡面塗料等。
  8. In this sense, ida plays dual effects, tridentate chelating to cu ( 2 ) and bridge between two cu ( 1 ) andcu ( 2 ). 3 metal complexes were selected as the appropriate for the study of cleavage plasmid pbr322dna by gel electrophoresis technique. the results showed ni and mn complexes could cleave effect - ively dna in the presence of h2o2 at physiological ph and temperature, whereas individual zn complex could cleave effectively dna

    通過電泳實驗研究了一系列金屬配合物與pbr322dna的作用，發現在tris - hcl緩沖溶液中，生理條件下，鎳、錳配合物在共反應物h _ 2o _ 2存在下能夠很好的斷裂dna ，而zn配合物單獨作用就能夠使dna由ccc型轉化為oc和linear型。
  9. In order to produce monoclonal antibodies, first, several v. dahliae isolates were grown in liquid czapeak medium, after rinsing mycelia and eliminating zoospores, the fungal tissue was homogenized with the pestle in liquid nitrogen and then transferred to test tubes and was centrifuged in tris - hcl buffer

    在制備抗原的過程中，首先液體振蕩培養了若干株棉花黃萎病菌，經過沖洗除孢子、液氮研磨，用tris - hcl抽提，再離心制得菌絲蛋白提取液，可作為電泳樣品。
  10. Using diethanolamine as aminating agent and glacial acetic acid as neutralizing agent, aminated epoxy acrylic cationic resin was prepared. the effect of technology of aminated epoxy acrylic resin on properties of eletrodeposition was studied by conductivity meter and electrophoresis apparatus. it was shown that, conductivity firstly decreased, and then increased with aminating temperature increase. in contrast with putting polyacrylic resin into thin acetic acid solution, the more compact film could be achieved by neutralizing polyacylic resin with glacial acetic acid and then add it into water. when neutralizing temperature was enhanced, the speed of electrodepsidon was found to increase, and the film was also more compact. increasing the dn leads to enhanced conductivity and smaller particle size. when dn equaled to 80, the smoothest film could be achieved

    以二乙醇胺為胺化劑、冰醋酸為中和劑，合成了胺化環氧丙烯酸陽離子樹脂.採用電泳儀和電導率儀，研究了胺化環氧丙烯酸樹脂合成工藝對陰極電泳塗料電沉積性的影響.結果表明，隨著胺化溫度的增加，電泳液電導率先下降後上升.將冰醋酸加入樹脂中中和，後用水稀釋，比樹脂在醋酸稀溶液中中和，電沉積性能更好.電沉積速率隨著中和溫度的上升而增加，電沉積膜緻密性相應增加.中和度（ dn ）愈高，電泳液電導率愈大，粒徑越小，而塗膜外觀在中和度為80時達到最佳
  11. Medium experiments were arranged under uniform design, and then an optimum medium was got accordingly. the culture liquid was centrifugalized at 3, 500r / min for 30min, then ammonium sulfate was added into the supernatant to a final concentration of 30 % to precipitate the others

    通過硫酸銨分級沉澱、 deaesephadexa - 50陰離子交換凝膠層析和sephadexg - 75凝膠柱層析對發酵液進行分離和純化，並得到電泳純的酶。
  12. It was found that b - 6 isolated by the method of distinct zone of clearing in cellulose - congo red agar medium combined with measuring the enzyme activity of liquid culture filtrates had comparatively higher cellulase activity. by measuring activity of cellulase of strains growing in medium with different carbon sources and of washed mycelium induced by different carbon sources in certain time, it found that the formation of cellulase was regulated by the nature of the carbon source used for b - 6 and as3. 3711

    真菌纖維素酶是一種誘導酶，碳源同時也是主要的誘導物來源，為了研究碳源對真菌纖維素酶合成的誘導機理，本文利用液體生長培養和洗滌菌絲誘導培養法研究了不同碳源對兩菌株的誘導特性，並用電泳分析法研究了不同碳源的誘導酶譜。
  13. Purification and characterization of phytase from a. niger an 01001 a. niger an01001 was inoculated on solid media and cultivated at 30 for 5 days. proteins were extracted from solid - state fermentation with 50mm acetate buffer ( ph5. 5 ). the molecule weight of the phytase protein was determined as about 78kd by sds - page. the purification procedures include ammonium sulfate precipitation, deae - cellulose ion - exchange chromatography, gel electrophoresis and electroelution

    3 .植酸酶的分離純化及其性質研究黑麴黴ano1001經固體發酵，用緩沖液抽提后，經硫酸按沉澱， deae一纖維素離子交換層析，聚丙烯酞胺凝膠電泳和電洗脫等純化步驟獲得的植酸酶，用sds一page檢測為一條均一譜帶，其分子量約為78kd 。
  14. Determination of sucrose in poly - - glutamic acid fermentation liquor by capillary electrophoresis with uv detection

    毛細管電泳紫外檢測聚谷氨酸發酵液中蔗糖濃度
  15. On the basis of the conditions of diphenyl ( dp ), ortho - phenyl phenol ( opp ), thiabendazole ( tbz ), imazalil ( imz ) application for fruits and vegetables preservative, the analytical methods for residues and their progresses are summarized

    摘要結合防腐劑聯苯、鄰苯基苯酚、噻苯咪唑、抑霉唑在水果蔬菜中的使用狀況，綜述了果蔬中防腐劑殘留分析方法及其進展，並著重對氣相色譜、液相色譜及防腐劑殘留分析新技術毛細管電泳技術、免疫分析法等進行闡述。
  16. Sh chou 、 t kno 、 m lin 、 ok lee ， " in utero transplantation of human bone marrow - derived multipotent mesenchymal stem cells in mice. " ， journal of orthopaedic research ， vol. 24 ， no. 3 ， march 2006 ， pp. 301 - 312

    方晴、周秀慧、柳如宗、林震煌，毛細管電泳/線上掃集濃縮技術對老鼠血液中麥角乙二胺的分析與研究，第63卷，第1期， 2005年3月，頁77 - 86 。
  17. The results of lauryl sodium sulfate - polyacrylamide gel electrophoreses ( sds - page ) of the aggregate precipitate and supernatant and the result of high - performance size - exclusion chromatography of the supernatant indicated that, by wrongly linked intermolecular disulfide bonds soluble bi - molecular and tri - molecular egg white lysozyme aggregate could be simultaneously formed except being renatured to native and active egg white lysozymes during the refolding procedure of denatured - reduced egg white lysozyme ; the aggregate precipitate could be further formed by the non - covalent bonds interaction between the soluble hi - molecular egg white lysozyme aggregates, and the soluble tri - molecular egg white lysozyme aggregate could still stay at the supernatant

    沉澱和上清液的不連續十二烷基硫酸鈉聚丙烯酰胺凝膠電泳（ sds - page ）和高效凝膠排阻層析分析結果表明，還原脲變性蛋白溶菌酶在稀釋復性過程中除了能夠復性成天然態蛋白溶菌酶分子外，還會形成可溶的蛋白溶菌酶分子二聚體和三聚體，二聚體和三聚體主要是靠分子間二硫鍵的錯配連接而成的；可溶的蛋白溶菌酶分子二聚體之間通過非共價鍵相互作用而形成集聚體沉澱，而可溶的三聚體溶菌酶分子則仍處于復性液上清液中。
  18. To find dnaase in the earthworm the tissue extract of earthworm with different concentration reacted with rna, circular dna, linear dna for an hour at 37, then the producetion was detected by 1 % agrose gel. the tissue extract of earthworm, the tissue protein extract of earthworm and the tissue extract of earthworm without protein reacted with pbv220 - r - inf for an hour at 37, then the producetion was detected by 1 % agrose gel. 2

    雙胸蚓組織中dna酶的發現用不同濃度的雙胸蚓組織提取液與rna 、環狀dna及線狀dna在37反應1小時後用1的瓊脂糖凝膠電泳對其反應產物進行觀察；雙胸蚓組織粗提取液、雙胸蚓組織蛋白粗提取液及雙胸蚓組織去蛋白提取液分別與pbv220 - ? inf質粒37反應1小時後用1的瓊脂糖凝膠電泳對其反應產物進行觀察。
  19. It owns more than 50 precision instruments, such as lc - ms, gc - ms, hpce, hplc, gc, ft - ir, uv, tlcs, aa, etc. there are about 15 thousand chinese and foreign books on relative specialities and more than 140 sorts of chinese and foreign journals in the library

    擁有液-質及氣-質聯用儀原子吸收分光光度計毛細管電泳儀高效液相色譜儀氣相色譜儀紅外分光光度計薄層掃描儀全自動溶出儀等大型精密儀器150多臺件。
  20. X - ray photoelectron spectra ( xps ) and fourier transform infrared ( ftir ) have been used to analyze the deposited ps films

    取上層懸液作電泳液，鋁片分別作正、負極， 200v cm電場下電泳20min 。
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