電泳現象 的英文怎麼說
中文拼音 [diànyǒngxiànxiàng]
電泳現象
英文
electrophoresis-
Results : there was no contorting phenomenon of hemoglobin in pisces cyprinus carpio and amphibia rana catesbeiana in the the course of " direct chiasma " 、 " crossing " and " surrounding " experimentation
結果：鯉魚、牛蛙的血紅蛋白自身在「直接交叉」 、 「穿過」 、 「兜過」實驗電泳過程中均未出現扭曲現象。( 2 ) the mortality of cp cell in 10 were increasing directly related with the time in the low temperature. after 120d some cp cells died in the way of apoptosis. most nucleus of cells were condensed, and chromosome was marginated beside the nucleus inner membrane, cell sizes were reduced, dna ladder showed in dna gel electrophoresis
（ 2 ） cp細胞系在10低溫下細胞死亡率與時間成正比， 120d的細胞具典型的凋亡現象，即細胞核固縮、染色體邊集在核膜內側；細胞體積變小；瓊脂糖凝膠電泳上顯示特徵性的「梯狀」帶。After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s
通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。Multiple organ comet assay in vivo is very useful for the evaluation of genotoxicity target organ. the comet tail of peripheral lymphocytes is suitable as a biomarker for reflecting the sensitivity of organs after treatment of mtx
提示，體內多器官的單細胞凝膠電泳分析對探測體內遺傳毒性靶器官是十分有用的，外周血淋巴細胞在scge分析中的拖尾現象可作為用藥后組織器官對藥物敏感性反映的生物標志。The mechanisms of such treatment have been proposed as inhibition of proliferation and angiogenesis, as well as induction of differentiation and apoptosis, as has been tested by various in vivo and in vitro experiments. in our experiments, it has also been demonstrated that after the treatment of arsenic trioxide, the k562 cells has undergone major morphological changes, which included nuclear shrinkage, membrane bleb and scattered apoptotic bodies. dna gel electrophoresis also discovered that the typical " dna ladder " phenomena in the treatment group, while the control group showed the regular genomic banding
我們在實驗中觀察到as _ 2o _ 3作用人紅白血病k562細胞后，細胞生長明顯變緩，部分細胞出現皺縮、染色質濃聚及胞膜起泡現象，部分細胞胞膜破裂，在其周圍有緻密的凋亡小體出現， dna電泳出現典型的凋亡「梯狀」帶，提示as _ 2o _ 3能有效抑制k562細胞生長，誘導k562細胞凋亡。The approaches used in the study included : observing the microstructure and ultrastructure of the cell line of colossoma brachypomum ( cbt ) and the cell line of carp ( cp ) stressed low temperatures under fluoroscopy and tem ; analysis of dna damage in the cultured cells under temperatures stress by dna gel electrophoresis
本研究採用的主要實驗方法：通過熒光顯微觀察、電鏡超微結構觀察確定cbt （淡水白鯧臀鰭細胞）和cp （草魚胚胎細胞）在低溫處理后的顯微與超微結構的變化。應用dna電泳分析細胞dna在低溫處理后的斷裂現象。A mixture of three amino acids ( arg, gly, glu ) labeled with fluorescein isothiocyanate ( fitc ) was separated in pdms microfluidic chip, the separation voltage is 200v / cm, the separation time is less than 120 seconds ; according to ccd fluorescence images, two distinct physical processes - stacking and destacking during sample injection were studied qualitatively ; rhodamine b, a kind of temperature - dependent fluorescence dye, was used as probe to develop a temperature - fluorescence intensity equation, then temperature - color map in microchannels was constructed, and temperature trait in microchannels on the pdms microfluidic chip was analysed. according to the results, we conclude that the electric field applied to the pdms microfluidic chip should not exceed 400v / cm
利用pdms微流控晶元對fitc標記的精氨酸、甘氨酸、谷氨酸混合物進行了電泳分離，分離電壓為200v cm ，分離時間不到120秒；通過拍到的熒光顯微圖像對電泳注樣過程中復雜的樣品分子積聚與解聚現象作定性的分析；以熒光染料rhodamineb為溫度熒光探針，建立了pdms微流控晶元上的溫度-熒光強度的關系公式，並利用matlab圖像處理工具箱構建出微流體溝道內的溫度色圖，對pdms微流控晶元的微流道溫度特性進行了分析，根據實驗結果，我們認為對于pdms微流控晶元來說，在進行需要外加電場作用的試驗時，外加電場不應超過400v cm 。When it was verified to be pure by sds - page, page and clc, it could be concluded that hq - 5 ' s autoproteolysis just was the cause of the proteolytic phenomenon of eluant of the thiophilic adsorption chromatography hq - 5 was identified as a glycoprotein containing 3. 5 % neutral carbohydrates
由此可斷定嗜硫色譜洗脫峰的降解現象是由hq - 5的自降解引起的。 hq - 5經sds - page電泳測得分子量為47 . 6kda ，含有鏈內二硫鍵，加dtt處理后，分子量為50 . 8kda 。All groups of microtus fortis showed polymorphism at most of the ten loci investigated. the electrophoretic mobilities of the small voles from heilongjiang revealed great divergence with the other three groups : the small voles from heilongjiang had their particular alleles at six loci ( es - 1, hbb, gpi, akp - 1, trf - 1, ce - 2 ) and the phenotypes of them were completely different at three ( es - 1, hbb, gpi - 1 ) of these loci. the genetic distances among the voles from hunan, ningxia and the big voles from heilongjiang were ranged from 0. 0633 to 0. 2107 while that between the small voles from heilongjiang and the other three groups were ranged from 0. 7068 to 0. 8953
結果表明：四類東方田鼠在所觀察的10個生化位點上，大部分都存在著多態性現象；黑龍江小體型東方田鼠與其它三類東方田鼠的電泳結果差異較大，在es - 1 、 hbb 、 gpi - 1 、 akp - 1 、 trf 、 ce - 2六個位點土都存在其所特有的等位基因，其中es - 1 、 hbb和gpi - 1三位點上它的基因型與其它三類鼠完全不同；湖南、寧夏和黑龍江大體型東方田鼠三者間的遺傳距離在0 . 0633 0 . 2107之間，而黑龍江小體型東方田鼠與其它三類鼠的遺傳距離在0 . 7068 0 . 8953之間。分享友人