電轉染 的英文怎麼說

中文拼音 [diànzhuǎnrǎn]
電轉染 英文
electrotransfection
  • 電 : Ⅰ名詞1 （有電荷存在和電荷變化的現象） electricity 2 （電報） telegram; cable Ⅱ動詞1 （觸電） give...
  • 轉 : 轉構詞成分。
  • 染 : Ⅰ動詞1 （用染料著色）dye 2 （感染） catch [contract] （a disease） 3 （沾染） acquire （a bad hab...
  1. In order to find out the mechanism of bone growth and biodegradation of this kind materials animal experiment was adopted in this paper, by use of sem, epma and polarizing microscope it discussed the transformation of porous bioceramic after implanted in rabbit ' s femur. in this experiment we got some important findingsfirstly, after implanted the material began to degrade indeed

    利用掃描電鏡、電子探針、 x光片以及甲苯胺藍和he染色等組織學觀測手段，本文探討了- tcp多孔生物陶瓷在植入骨內后結構形態與組成的變化，深入分析了- tcp多孔生物陶瓷的降解機理和晶體轉變過程。
  2. The results of these early research work showed that rna polymerase iii transcription was localized in the nucleoplasm. however, with the development and the application of new technologies since 1990s, the controversy arose on the transcription sites of rna polymerase iii. in recent years, more and more scientists presumed that rna polymerase iii transcription might not occur in the nucleoplasm but in the nucleoli

    自上個世紀八十年代初期，人們相繼運用細胞化學染色、電鏡放射自顯影等進行研究的結果表明： rna聚合酶的轉錄發生在核質中，但隨著新的研究技術的發展和應用，人們卻發現rna聚合酶的轉錄可能發生在核仁中，從而對早期的研究結果提出了質疑。
  3. During the different time point, we retrieve the cell supernatant and through the electrophoretic analysis and immunoblot of the recombinant protein indicated that the cell which transfected the recombinant eukaryotic gene expressing vector could synthesis and excrete the peptide effectively

    提取培養細胞總rna反轉錄cdna測序結果表明人生長激素基因組dna在家蠶bmn細胞內能正確轉錄，剪接。蛋白質電泳分析和免疫學檢測證明轉染細胞能夠有效合成並分泌hgh蛋白質。
  4. ( 8 ) by studying match between the electronic system and catalytic converter on the base of electronic controlled bypassing air system, conclusions having been gotten as follows : higher conversion efficiency of emission have gotten when a / f fluctuates at definite scope and frequency, by controlling the comparing voltage of 02 sensor, the working scope of catalytic converter can be controlled and the matching that can fulfill the high efficiency of hc, co, nox at same time has been optimized

    （ 8 ）進行基於所開發的電控補氣系統上的催化器與電控系統的匹配研究，當控制系統調節空燃比在一定范圍按照一定頻率進行波動時，可以提高催化轉化器的轉化效率；通過控制氧傳感器的比較電壓，可以控制催化轉化器的工作窗口，實現排放污染物中， hc 、 co 、 nox轉化的最優匹配。
  5. After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s

    通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。
  6. The artificial antibacterial peptide - ceme gene was designed according to the codon with the highest frequency in pichia pastoris, then the ceme gene was integrated into the chromosome of pichia pastoris strain - gsl 15 by electroporation

    我們根據人工設計的新型抗菌肽ceme序列設計ceme基因，重組到酵母胞內表達型的整合載體phild2中，通過電轉化作用將ceme基因整合至酵母宿主gsll5染色體上。
  7. This machine has high rotational speed, it is based on the common centrifugal separator, its novel design great enhances the speed of lowing the supplies. main applies : this machine is called throw liquid centrifugal separator, is suitable for clarifying drizzle drops of liquid that has floated, now it has been widely applied in the industries of medicine, chemistry and food industry, it can be used to separate materials before the process of the centrifugal separator of pipe type

    該機適用於對固相為顆粒狀的懸浮物的過濾，也可用於纖維及其紡織印染洗滌後有脫水，物料從頂部加入，由專用雙速電機通過三角膠帶，實現低速啟動，高速分離，使轉鼓形成離心力場，液相通過固相和濾網由下部出液口排出，固相停機後由上部取出。
  8. It was then cloned to the secreted vector - ppic9k and recombined successfully into the chromosome of pichia pastoris host strain - gsl 15 by electroporation

    通過電轉化作用該基因片段被成功地整合至酵母cs115的染色體上，經過甲醇誘導之後，該基因得到了分泌表達。
  9. A. in aerobic bioremediation, oxygen is the electron acceptor, and is required for the oxidation - reduction reactions that transform the organic contaminants ( petroleum hydrocarbons ) to carbon dioxide and water

    答：在有氧生物降解過程中，氧是電子受體。原先的有機污染物（石油烴類）被轉化為二氧化碳和水。
  10. Electricity generation in hong kong has produced significant air pollution, threatening our health. it has also contributed significant amounts of greenhouse gas emissions, contributing to global warming and climate change, the single biggest threat to our planet and biodiversity

    香港的電力生產過程構成嚴重的空氣污染，同時排放大量溫室氣體，導致全球氣溫上升和出現氣候轉變，成為地球和生物多樣性面對的最大的威脅。
  11. This paper introduce the features of a kind of electric heating atmospheric boiler which is of high efficiency on energy transferring, non - pollution, starting and stopping quickly, wider range of load adjusting, simple body structure, etc. in addition the paper introduce the design idea briefly

    電加熱鍋爐具有能量轉化效率高、零污染、起停速度快、負荷調節范圍大、本體結構簡單等特點，文章具體介紹了一種常壓熱水電鍋爐的設計。
  12. Dna and rna dot blotting revealed that the f gene was transcribed into mrna in the vero cells. there was expression of the f protein as shown by indirect immunofluorescent assay. the expression began at 48h post - infection and increased thereafter, as indicated by elisa

    將真核表達質粒pcdna3 - f高壓電轉化dam和phop基因雙突變的減毒鼠傷寒沙門氏菌zj111株（ zj111 / pcdan3 - f ） ，並直接轉染vero細胞，分別提取細胞總dna和總rna ， dig標記探針均可檢測到陽性雜交信號。
  13. In spite of the low transition efficiency, the thermoelectric transition equipments have a lot of characteristics such as no moving parts, pollution and noise, long life span and safety, which stirs people to research them with great enthusiasm

    熱電轉換裝置盡管目前轉換效率比較低，但因為具有結構緊湊、沒有移動部件、工作無噪聲、使用壽命長、安全不失效、易於自動檢修、無污染等優點，從而激起了人們的研究熱情。
  14. According to the gene sequence and secondary structure of hcv ns5b, we design the sirnas targeting ns5b gene following with the requirement for sirnas design from tuschl et. al and synthesize it from dharmacon company ; hepg2 cell stably expressing ns5b - egfp protein was trasfected by synthesized sirnas with electroportion, the non - transfected cell and non - specific sirnas transfected cell are c onsidered as control group ; inhibitory effect of sirnas was investigated by fluorescence microscope with dapi dyeing and by semi - quantitative rt - pcr

    然後根據dsrna設計原則，結合nssb基因的序列特徵，藉助生物信息學軟體設計了針對nssb基因的sirnas ，並交由公司化學合成；電穿孔法轉染上述穩定轉染的細胞克隆，同時分別以非特異的sirnas轉染組和空白轉染組為對照， dapi染色后通過熒光顯微鏡和內標化rtpcr檢測，初步證實了化學合成的sirnas可以特異阻斷nssb基因的表達。
  15. A deletion recombinant virus ( hasnpva132 ) of hal32 was generated by homologous recombination in e. coli. electron microscope pictures revealed the deletion virus could replicate in hzaml cells, which indicates hal32 is not essential for the replication of hasnpv

    通過在大腸桿菌內同源重組構建得到orf132的缺失重組病毒（ hasnpv凸132 ） ，轉染成功后的電鏡切片表明， hasnpv凸132能夠在hzami細胞內正常復制、增殖。
  16. After multiple, plasmid ploxifn and pbs185 dna were co - transfected into the green fluorescent cell clones, then filtrated the non - fluorescent cell clones. two non - fluorescent cell clones, the green fluorescent cell clones and the cells which were co - transfected by the plasmid ploxifn and pbsiss dna were checked up by pcr

    首先將質粒ploxgfpdna電轉染牛胎兒成纖維細胞，並用g418篩選，篩選出綠色熒光細胞克隆，增殖培養之後，將質粒ploxifn和pbs185dna共轉染熒光細胞克隆，篩選出不發光的克隆。
  17. In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ), and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee. the plasmid pfb 1 - cee was transformed into dh1 obac competent e. coli cells. high molecular weight dna was prepared from the overnight cultures from the selected e. coli colonies, which was recombinant baculovirus shuttle vector containing hcv structural gene, named bac - cee

    本實驗用pcr擴增hcv結構區基因，克隆到桿狀病毒表達載體pfastbacl中，構建成重組轉座載體pfb1 - cee ，轉化dh10bac大腸桿菌感受態細胞，篩選陽性菌落，抽提大分子質粒dna ，獲得含hcv結構區基因的重組桿狀病毒穿梭載體bac - cee ，脂質體介導轉染sf9昆蟲細胞，出現細胞病變后，收集含有重組桿狀病毒顆粒的培養上消，重新感染sf9細胞，收集sf9細胞，進行12 . 5 sds -聚丙烯酰胺凝膠電泳，可見表達的蛋白條帶。
  18. In this dissertation, the plasmids containing 5s promoter were transfected into hela cells, the transcription sites of rna polymerase iii and its transcripts were detected by fluorescence in situ hybridization ( fish ) to dna, rna and dna - rna, respectively

    本實驗以人的hela細胞為材料，運用電擊轉染、熒光原位雜交並結合激光共聚焦顯微鏡，從dna 、 rna和dna - rna三個水平對rna聚合酶的轉錄位點及其轉錄子的分佈進行研究。
  19. Present research proved that it is reasonable to explore es cell committed induction pathway by transfecting special transcript factor, maker gene or relative cell differentiation factors combined with reporter gene and selecting method with inducer

    1作載體，電轉方法對es細胞進行轉染，以18進行篩選。 2 . rt一pcr方法鑒定ibob基因的插人和表達。
  20. To confirm the approval of recombinant pcdnas - tgfjtf, gene and the results of its transfection, we used immuhistochemical staining ( sabc ). the department of or a logy 4 multiplication and differentiation of bmscs were observed by flow - cytometry ( tcm ), transilluminating electron microscope ( tem ) and other methods. the bmscs in controlled group was transfected with pcdna3 only

    採用電泳檢測pcdna3 - tgf _ 1構建是否成功；通過tgf _ 1免疫組化染色檢測轉染是否成功；運用形態學觀察、透射電鏡（ tem ） 、流式細胞儀（ fcm ） 、堿性磷酸酶（ alp ）染色、型膠原免疫組化染色等方法，觀察轉染后bmscs增殖與分化情況。
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