青霉素酶 的英文怎麼說

中文拼音 [qīngméisù]
青霉素酶 英文
neutracillin
  • 青 : Ⅰ形容詞1 （藍色或綠色） blue or green 2 （黑色） black : 青布 black cloth; 青牛 black ox3 （年輕...
  • 霉 : Ⅰ名詞（黴菌） mould; mildew Ⅱ形容詞（變質） mouldy; mildewed
  • 素 : Ⅰ形容詞1 （本色; 白色） white 2 （顏色單純） plain; simple; quiet 3 （本來的; 原有的） native Ⅱ名...
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  1. E. coli xl1 - blue cells were tansformed by psurfpga and phages were rescued by m13ko7 helper phage particles. results showed that the heterodimeric enzyme was expressed as a fusion protein that matures to an active biocatalyst connected to the coat protein of phage fd

    以構建的噬菌粒psurfpga轉化具有琥珀突變的大腸桿菌xl1 - blue ，以輔助噬菌體m13k07超感染，進行青霉素g酰化酶基因的表達和在噬菌體表面的展示。
  2. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  3. Considering alcaligenes faecalis pencillin g acylase ( afpga ), which possesses the attractive characteristics for beta - lactam antibiotics conversions, the gene of pga was cloned into two expressing vector pkkfpga and psmlfpga. both the constructed plasmids psmlfpga and pkkfpga contained the pga gene, trc promoter and rrnb transcript terminator, differ in the replicon and antibotic marker, pkkfpga contained multicopy replicon ( cole 1 ) and ampicillin marker. while psmlfpga contained medium - copy replicon ( p15a ) and tetracycline marker

    本文將糞產堿桿菌青霉素g酰化酶（ afpga ）基因構建表達載體pkkfpga和psmlfpga ， pkkfpga和psmlfpga均含trc啟動子、青霉素g酰化酶基因、 rrnb轉錄終止子，其中pkkfpga含有氨卞青霉素抗性基因和cole1高拷貝復制子；而psmlfpga含有四環素抗性基因和p15a中拷貝復制子。
  4. The results obtained were as follows : the mic of engineered peptide against staphylococcus aureus atcc 25923 ( penicillin sensitive strain ), atcc 29213 ( penicillinase - producing strain ) and atcc baa - 42 ( methicillin resistant strain ) were 1g / ml, 2g / ml, 4g / nil respectively. pi staining showed the staphylococcus aureus treated with the engineered peptide were dead

    實驗結果表明:該工程多膚對三種atcc標準菌株atcc25923 （青霉素敏感菌株） 、 atcc29213 （產青霉素酶菌株） 、 atccbaa一42 （耐甲氧西林菌株）的mlc分別為1林留血l 、 2林留ml 、 4林創ml 。
  5. A new carrier for immobilization of penicillin acylase

    固定化青霉素酰化酶的新載體
  6. Progress in support material for the immobilization of penicillin acylase

    青霉素酰化酶固定化載體材料研究進展
  7. Studies on immobilization of penicillin acylase to chitosan as larrier

    以殼聚糖為載體固定化青霉素酰化酶的研究
  8. The purified enzyme had a specific activity of 68. 6 u / mg protein. overproduction of pga was often limited by translocation and / or periplasmic processing steps, subsequently resulted in intracellular accumulation of various types of pga precursors and then formed inclusion bodies in the cytoplasm and / or periplasm

    經deae - sepharosecl6b離子交換層析和butyl - sepharosecl4b疏水層析，即可得純度提高20倍、比活為68 . 6u mg的青霉素g酰化酶，兩步純化的總得率達91 。
  9. Immobilization of penicillin acylase by intensive adsorption of chitosan on celite

    硅藻土強化吸附殼聚糖固定化青霉素酰化酶
  10. Immobilization of penicillin g amidase on beaded glycidyl methacrylate copolymer support

    青霉素酰化酶在甲基丙烯酸縮水甘油酯共聚物上的固定化
  11. The supernatant fraction and the precipitation fractions were analyzed by western blotfor strain dh5 a / pkkfpga, 5 - 10 % pga precursors formed as inclusion bodies in the cytoplasm while no inclusion bodies formed in the periplasm, this suggested most pga precursors were transported to the periplasm and matured to active pga and indicated that the maturation of pga in strain dh5 / pkkfpga was limited by the translocation step

    Western印跡分析表明對于菌株dh5 pkkfpga ， 5 - 10的原前體青黴g素酰化酶在胞內形成了包涵體，說明其成熟的限速步驟在胞內的運輸階段，而菌株dh5 psmlfpga則無明顯包涵體形成，說明菌株dh5 psmlfpga改善了青霉素g酰化酶的合成流，因而其表達能力高於菌株dh5 pkkfpga 。
  12. Studies of a polyacrylic acid as a carrier for the immobilizat ion of penicillin acylase

    聚丙烯酸載體用於青霉素酰化酶的固定
  13. Studies on immobilization of penicillin acylase on novel complex carrier pei silica gel

    青霉素酰化酶在新型復合載體上的固定化研究
  14. Emmobilzation studies of penicillin acylase on the porous bead with oxirane groups

    以環氧乙烷為活性基的多孔顆粒狀固定化青霉素酰化酶的制備
  15. Twenty years later, my lab had outlined the structure and biosynthesis of the peptidoglycan of bacterial cell walls and had discovered that penicillin inhibited the terminal step in its biosynthesis catalyzed by transpeptidases

    20年後，我的實驗室揭示出了細菌胞壁肽聚糖的結構和生物合成過程，並發現青霉素是抑制由轉肽酶催化的該生物合成的最後一步。
  16. The interesting gene fragment with ecori and noti were amplified by overlapping pcr, which inserted into vector plasmid ppic9k after degisted by ecori and noti, and the recombinant plasmid was transformed into competent dh5cc. positive clones were screened by pcr from the lb plate with amp. digesting analysis resulte shows that the interesting gene were inserted into the vector ppic9k with correct direction

    目的基因經雙酶切后連接載體ppic9k ，然後導入大腸桿菌dh5中，在含氨卞青霉素（ amp ）的lb板上用pcr反應篩選出陽性菌落，雙酶切結果表明目的基因已插入載體中，且方向正確，測序結果進一步證明人巨細胞病毒重組基因表達質粒成功地克隆了目的基因片段。
  17. Most s. pneumoniae isolates were at least intermediately resistant to penicillin, and 10. 1 % of h. influenzae showed beta - lactamase activity

    大多數s .肺炎隔離群對青霉素的抵抗都致少居中，並且10 . 1的h .流感顯示了-內酰胺酶活性。
  18. First, the purified pezzis and pcr product of angiostatin are digested by ecor. i and xba i. after purifying the digested products respectively, we ligate these two kinds of dna by t4 dna ligase and construct the recombinant plasmid pezz18 - as. then transform it to the competent e. coli dh5a

    用限制性內切酶ecori與xbai對目的基因as 、表達載體pezz18行雙酶切，酶切產物純化后利用大腸桿菌t _ 4dna連接酶連接構成重組子pezz18 - as ，並轉化e . colidh5 ，經氨芐青霉素lb平板初篩后，以菌液pcr和重組子的單、雙酶切行進一步鑒定。
  19. Exposure of cells to the fungal inhibitor wortmannin eliminated focus formation by all repair factors examined, suggesting a role for the phosphoinositide ( pi ) - 3 family of protein kinases in mediating this response

    抗真菌藥物渥曼青霉素作用於細胞，能通過檢測到的修復因子消除形成的病灶，這說明了蛋白激酶中磷酸肌醇家族在這一反映中所起的作用
  20. The trace penicillin in milk is detectd by microbiosensor, potentiometric enzyme electrode and amperometric enzyme electrode respectively. the most efficient, economical and feasible measurement will be screen out

    嘗試用微生物傳感器、電位型酶傳感和電流型酶傳感器測定牛奶中殘留青霉素，摸索經濟、高效的測量方法，篩選出可實用的傳感器
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