預電泳 的英文怎麼說

中文拼音 [yùdiànyǒng]
預電泳 英文
prerunning
  • 預 : Ⅰ副詞（預先; 事先） in advance; beforehand Ⅱ動詞（參與） take part in
  • 電 : Ⅰ名詞1 （有電荷存在和電荷變化的現象） electricity 2 （電報） telegram; cable Ⅱ動詞1 （觸電） give...
  • 泳 : 動詞（游泳） swim
  1. This system contains : parts load, pre - degrease, degrease, spray cleaning, spray surface adjustment, spraying phosphating, rinse with d - ionized water, electro - deposition, ultra - filter for post rinse, oven curing, cooling and unload

    由上件，預脫脂，脫脂，噴洗，噴淋表面調解，噴淋磷化，去離子水噴洗，電泳塗裝，超濾液噴洗，漆膜固化，自然冷卻，下件工序組成。
  2. Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry

    三、 gst一hbrp重組蛋白的純化1 .超聲破碎細胞，離心，上清和沉澱進行sds一page電泳分析2 .樣品處理提取包涵體，變性后，加人用pbs平衡過的以utal腸onesepb抓脫4b ，室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據毛山lel ，決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積，用預冷的1xpbs清洗cldtathionese戶， se4b ，得到50 %的基質
  3. Materials and methods in our study, first the e. coli bl21 transformed already are cultivated in smaller scale and then the plasmids dna were extracted by the methods of alkaline lysis. the plasmids dna extraction were processed 37 overnight by the restrictive endo - incisase bam h i and xho i. the incision products were used to check the integrality and variability of the recombinant plasmids dna by 1 % agarose gel elec - trophoresis

    同預想結果基本吻合，大規模培養后純化得到的融合蛋白通過sds page顯示在52kda處有一條帶，而酶切后產物電泳結果顯示在26kda處分另有兩條蛋白帶，其中一條為gst ，另一條為hbrp ，基本符合實驗結果； hbrp對tpk活性的抑制呈劑量依賴性。
  4. The specific band was excised from the gel and injected into mice. the antiserum was collected from the immunized mice and used for indirect fluorescent assay ( ifa ) with chicken embryonic firoblast ( cef ) infected by mdv

    誘導菌體的裂解物經12的sds聚丙烯凝膠電泳（ sds - page ）和western - blot試驗驗證，得到大小為43kd的融合蛋白，與預期大小一致。
  5. Location of the hbrp gene in the chromosome according to stanford g3 rh panel, a pair of primers are designed in the 3 " - utr region of the gene which is to be localized and human genome dna is amplified in advance whose products are examined by electrophoresis. then g3 panel is amplified and this process is repeated twice. the pcr result is sent to stanford human genome center ( shgc ) http : / / www - shgc

    Hbrp基因的染色體定位使用stanfordg3rhkadiationhybrid ）嵌板，在所要定位基因的3 』非翻譯區（ untranslatedregion ， utr ）設計引物，預先擴增人基因組dna ，電泳檢測產物大小，繼之擴增g3rhpanel ，重復兩次，將pcr結果輸人斯坦福人類基因組中心（ shgc ）的主頁（ http ： 、 shgc
  6. This review summarizes the progression in preparation and preprocessing technologies of biological specimens. it especially introduces the preprocessing technologies, including centrifugation, filtration, dielectrophoresis, immunomagnetic separation, nucleic acid extraction

    摘要介紹生物標本的制備與預處理技術的進展，其中，重點介紹生物標本的預處理技術，包括離心分離、過濾、兩相電泳、免疫磁性分離及核酸抽提方法。
  7. The pcr products of gyra of a resistant strain ( sll - 3 ) was sequenced and analysed. when compared to the corresponding sequence of the gyra of salmonella typhimurium from the nucleotide sequences data of the gyra reported by griggs, 5 mutants sites were found in the qrdr. dma sequencing identified in the strain sll - 3 ser to phe change at codon 83 of gyra gene

    對所試菌株染色體的pcr產物行hinfi酶切后電泳檢測表明， cip高敏菌株（ 6株）得到預期大小為363bp 、 206bp 、 99bp的3個條帶；而耐藥菌株和中敏菌株（ 10株）電泳檢測到大小為363bp和305bp的2個條帶。
  8. The homologious comparison proved the cloned gene had 96 % homology to the sequence of the omp gene, and the alignment of the amino acid sequence was 98 %. the recombinant plasmid was constructed with the target gene and the expressing vector pgex - 4t - l and then was transformed into e. coli bl21 ( de3 ) the fusion protein was expressed under the iptg inducing condition, and exhibited about 62kda in size, very close to the predicted molecular weight of gst - momp. furthermore, the fusion protein was specifically recognized by anti - serum which raised against the major outer membrane protein of ahl316

    Sds - page電泳分析顯示誘導表達的基因產物分子量約為62kda ，與預測的gst -外膜蛋白重組融合蛋白的分子量極為相似， western - blot進一步證實，表達產物能被嗜水氣單胞菌l316主要外膜蛋白特異性抗血清所識別，產生明顯的染色條帶，說明所表達的基因產物與天然的外膜蛋白抗原性一致。
  9. On the bases of designing a primer pair, we obtained the coding domain sequence of rbp by pcr from cdna library. then the gene was cloned into pgem - t vector, the dna sequencing showed that the cloned gene was in agreement with the reported sequence. then the tageted gene of rbp was further subcloned into a procaryotic expression vector pbv220 and constructed recombinant plasmids was named pbv220 - rbp. in order to expresse rbp in procaryotic cell efficiently, the recombinant plasmids was introduced into e. colidhs a straints. expression of rbp was induced by temperature induction

    本實驗在合成該蛋白基因上下游引物的基礎上，利用pcr技術，從人睪丸cdna庫中釣取目的基因，並克隆于pgem - t載體中，經序列測定證明與文獻報道基本一致，再將目的基因從重組克隆質粒中亞克隆于pbv220原核表達載體中，轉化宿主菌，經溫度誘導后，進行sds - page電泳分析，發現在約21kd位置上出現了一條明顯的蛋白帶，與預期相符。
  10. Problems of sds - page and its preventive measures

    聚丙烯酰胺凝膠電泳常見問題分析與預防措施
  11. The purified products were cloned into pgem - t - easy vector successfully, the cloned plasmids were transformed into e. coli. tgl. the specific recombinant plasmid was identified by molecular weight, pcr and restriction endonuclease analysis. the results indicated that the resultant construct contained the gene of interest hn at right orientation of the insert

    經瓊脂糖電泳檢測，將大小與預計分子量一致的片段純化后連接到pgem - t - easy克隆載體中，再轉化大腸桿菌tgi感受態細胞，得到的轉化子經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  12. The purified production was cloned into pmd18 - t vector. the cloned plasmids were transformed into jm109. the specific recombinant plasimid was identified by molecular weight, pcr and restriction endonuclease analysis. the results indicated that the resultant construct contained the gene of ibdv a fragment at right orientation

    經瓊脂糖電泳檢測，將大小與預計分子量一致的片段純化后連接到pmd18 - t載體中，再轉化到jm109感受態細胞，得到的轉化子經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆，結果表明，得到的陽性重組子中含有a節段全長基因，插入到載體中的方向正確。
  13. Micro - channel electrophoresis chip ( mce chip ) system is a newly developed research field. the micro - channel network on the chip is manufactured by the technology of micro opto electro mechanical system ( moems ) on the substrate of glass, fused silica, pdms etc. sample separation is performed in the micro - channel and detected by opticcal or chemical methods

    微通道電泳晶元（ micro - channelelectrophoresischip ， mcechip ）系統是利用微光機電系統（ moems ）技術在玻璃、石英、有機聚合物等基片上刻蝕出預設計好的微通道網路，並在其中進行樣品的電泳分離，利用光學或化學等方法進行檢測。
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