顆粒狀載體 的英文怎麼說
中文拼音 [kēlìzhuàngzǎitǐ]
顆粒狀載體
英文
pelleted substrate- 粒 : Ⅰ名 (小圓珠形或小碎塊形物) small particles; grain; granule; pellet Ⅱ量詞(用於粒狀物)
- 狀 : Ⅰ名詞1 (形狀) form; shape 2 (情況) state; condition; situation; circumstances 3 (陳述事件或...
- 載 : 載Ⅰ名詞(年) year : 一年半載 six to twelve months; six months to a year; 三年五載 three to five ...
- 體 : 體構詞成分。
- 顆粒狀 : [攝影學] graininess; granulate顆粒狀混濁 granular cloud; 顆粒狀燃料 granular fuel
- 顆粒 : 1 (小而圓的東西) pellet; bead; dust; anything small and roundish (as a bean pearl etc )2 (一...
- 載體 : [化學] carrier; supporter; isotopic carrier
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Standard test method for particle size distribution of granular carriers and granular pesticides
粒狀載體與粒狀農藥顆粒大小分佈的試驗方法Then using ecbp21 antibody and immunogold transmission electron microscopy method, we studied the subcellular localization of ecbp21. the results indicated that the gold particles were mainly localized in the cell wall in callus cells and rachis cells of angelica dahurica. these results indicated that ecbp21 mainly localized in cell wall, which provide a direct evidence of the extracellular existence of ecbp21. furthermore, using ecbp21 antibody and immunohistochemical method, we studied the organic specially distribution of ecbp21, the results indicated that ecbp21 distributed in all organize, but it distributed more in leave n flower rachis than in leafstalk and root
首先,構建了ecbp21表達載體,誘導了重組蛋白的表達,並通過膠回收法獲得了大量純化重組ecbp21蛋白,制備了高效價、高特異性抗體;隨后,利用ecbp21抗體,結合免疫膠體金電鏡定位技術進行了ecbp21亞細胞定位研究,結果顯示:在白芷愈傷組織細胞和花序軸細胞中金顆粒主要分佈在細胞壁區域,而在細胞內未發現或僅有少量金顆粒分佈,表明ecbp21蛋白主要定位於細胞壁區域,這為細胞外cambp ( ecbp21 )的胞外存在提供了直接證據:進一步,利用ecbp21抗體,通過免疫組織化學分析研究了ecbp21組織特異性分佈狀況,結果表明ecbp21在白芷各組織中均有分佈,但在葉、花、花序軸中分佈較多,而在葉柄、根中分佈較少。In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ), and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee. the plasmid pfb 1 - cee was transformed into dh1 obac competent e. coli cells. high molecular weight dna was prepared from the overnight cultures from the selected e. coli colonies, which was recombinant baculovirus shuttle vector containing hcv structural gene, named bac - cee
本實驗用pcr擴增hcv結構區基因,克隆到桿狀病毒表達載體pfastbacl中,構建成重組轉座載體pfb1 - cee ,轉化dh10bac大腸桿菌感受態細胞,篩選陽性菌落,抽提大分子質粒dna ,獲得含hcv結構區基因的重組桿狀病毒穿梭載體bac - cee ,脂質體介導轉染sf9昆蟲細胞,出現細胞病變后,收集含有重組桿狀病毒顆粒的培養上消,重新感染sf9細胞,收集sf9細胞,進行12 . 5 sds -聚丙烯酰胺凝膠電泳,可見表達的蛋白條帶。
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