顯帶染色體 的英文怎麼說

中文拼音 [xiǎndàirǎnshǎitǐ]
顯帶染色體 英文
banding chromosome
  • 顯 : Ⅰ形容詞1 （明顯） apparent; obvious; noticeable; evident 2 （有名聲有權勢的） illustrious and inf...
  • 染 : Ⅰ動詞1 （用染料著色）dye 2 （感染） catch [contract] （a disease） 3 （沾染） acquire （a bad hab...
  • 色 : 色名詞[口語] （顏色） colour
  • 體 : 體構詞成分。
  • 染色體 : [生物學] chromosome染色體疾病 chromosomal disorders; 染色體異常 chromosome abnormality
  • 染色 : dye; dyeing; colouration; tintage; tinging; dyschroia; colouring; colour; [半] decoration染色不足...
  1. Karyotyping for adanced maternal age was performed by amniocentesis and demonstrated a normal male karyotype ( 46 xy with g - banding )

    對此高齡孕婦行羊膜穿刺染色體組型分型檢查，先是正常男性（ 46xy ， g帶顯帶技術） 。
  2. G - banding the gtg banding ( g - banding ) was carried out by the standard trypsin method with slight modification, which works well for protochordate because a good number of reproducible g - bands are consistently obtained from the embryonic cells of late blastulae and early gastrulae of amphioxus b. belcheri tsingtauense

    G帶型用稍作修改的標準的胰酶顯帶技術，進行gtg帶紋的顯示，它們能較好地顯示頭索動物青島文昌魚的晚期囊胚和早期原腸胚的中期染色體的g帶，並且重復性好。
  3. ( 2 ) the mortality of cp cell in 10 were increasing directly related with the time in the low temperature. after 120d some cp cells died in the way of apoptosis. most nucleus of cells were condensed, and chromosome was marginated beside the nucleus inner membrane, cell sizes were reduced, dna ladder showed in dna gel electrophoresis

    （ 2 ） cp細胞系在10低溫下細胞死亡率與時間成正比， 120d的細胞具典型的凋亡現象，即細胞核固縮、染色體邊集在核膜內側；細胞體積變小；瓊脂糖凝膠電泳上顯示特徵性的「梯狀」帶。
  4. Fos + / th + / gfap + and fos + / vp + / gfap + triple labeled n - asc could be found in the mvz, pvn and son respectively ; ( 2 ) under electronic microscope, the astrocytic processes connected closely with the dendrites or axons of the neurons, where the bilateral membranes became thick. we call transiently it electron - dense areas ( edas ). the number of edas increased remarkably following hyperosmotic stimulation ; ( 3 ) when trace retrogradely, wga - hrp was microinjected into the unilateral son, pvn or nucleus of solitary tract ( nts ) respectively using the stereotaxic method, the n - ascs formed by the neurons triple - labeled with hrp / fos / th ( or vp ) and astrocytes labeled with gfap could be found in the mvz, son and pvn respectively ; ( 4 ) after being treated with heperosmotic nacl solution, intracellular calcium concentration in cultured hypothamic neurons and astrocytes increased and then decreased

    腦內gfap陽性結構也明顯增多，其分佈與fos陽性細胞分佈基本一致，表現為胞體肥大、突起粗長； ast緊密包繞在神經元周圍形成神經元- ast復合體（ n - asc ） ；在mvz 、 pvn和son三重免疫組化染色切片上可見到fos + th + gfap +第四軍醫大學博士學位論文和fos vp gfap三重標記asc ； （ 2 ）免疫電鏡下son內星型膠質細胞突起與神經元樹突或軸突之間接觸部位出現增厚的膜結構一電于緻密區（ edas ） ，高滲刺激后數量明顯增多： （ 3 ）將們個mp注入大鼠一側n卜、卜卜或孤束核（ ws ） ，分別在延髓內臟帶（ mvz ） 、 so和pvn內出現fos hrp th 、 fos hrp八p三重標記神經元和gfap陽性標記ast形成的n asc ； （ 4 ）高滲刺激使培養神經元和ast內鈣水平先升高后降低，最後維持在比高滲刺激前稍高的靜息鈣水平上。
  5. The powder was then applied to a silica gel column chromatography charged with 80 % acetone in water and eluted with the same solvent. after concentrating and drying, relatively pure fr - 008 was obtained. hplc assay a comparison between antibiotic fr - 008 and candicidin has been made by studying the hplc separation profiles of antibiotic fr - 008, candicidin and a mixture of both

    對鏈黴菌fr - 008和灰色鏈黴菌imru3570的rflp分析發現，它們的染色體具有很高同源性； pfge的比較研究發現，這兩個菌株的明顯區別是鏈黴菌fr - 008攜帶有兩個線性質粒，而在灰色鏈黴菌imru3570中則沒有這兩個線性質粒。
  6. After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s

    通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。
  7. The mechanisms of such treatment have been proposed as inhibition of proliferation and angiogenesis, as well as induction of differentiation and apoptosis, as has been tested by various in vivo and in vitro experiments. in our experiments, it has also been demonstrated that after the treatment of arsenic trioxide, the k562 cells has undergone major morphological changes, which included nuclear shrinkage, membrane bleb and scattered apoptotic bodies. dna gel electrophoresis also discovered that the typical " dna ladder " phenomena in the treatment group, while the control group showed the regular genomic banding

    我們在實驗中觀察到as _ 2o _ 3作用人紅白血病k562細胞后，細胞生長明顯變緩，部分細胞出現皺縮、染色質濃聚及胞膜起泡現象，部分細胞胞膜破裂，在其周圍有緻密的凋亡小體出現， dna電泳出現典型的凋亡「梯狀」帶，提示as _ 2o _ 3能有效抑制k562細胞生長，誘導k562細胞凋亡。
  8. After g - banding, 65. 5 % of the chromosome surface is positively stained and the total number of g - banded is 149. there are 77 positive, 65 negative and 7 variable bands among the 149 g - bands

    經g顯帶技術處理后， 65 . 5的染色體區域呈陽性染色，共顯示了149條帶，其中陽性帶為77 ，陰性帶為65 ，可變帶為7 。
  9. A comparison of two methods for chromosome banding of peripheral blood lymphocyte

    兩種外周血淋巴細胞染色體顯帶方法的比較
  10. Methods karyotypes were analyzed by chromosome g - banding in 415 infertile patients with idiopathic azoospermia or severe oligozoospermia

    方法運用染色體g顯帶方法，對415例原發無精癥或嚴重寡精癥患者的核型進行分析。
  11. The trypsin treatment for g - banding is an important factor in obtaining well - banded chromosomes, while the slide aging is not

    G顯帶時胰酶的濃度及處理時間是獲得良好顯帶效果的關鍵因素，而染色體的片齡似乎並不太重要。
  12. Staining techniques which demonstrate structural transverse banding patterns are revealing less gross abnormalities and variations in chromosomes and proving valuable in relating normal and abnormal genes to particular sites on individual chromosomes ( gene mapping )

    顯示染色體結構橫帶構型的染色體技術可顯示較不明顯的染色體異常和變異，並證明在說明正常和異常基因與各個染色體特殊位置之間的關系（繪制基因圖方面）是有價值的。
  13. The nature of the mutant alleles is either nonsense mutation or the mutation which disrupted the splicing of the primary transcripts. anti - en antibody staining and in situ hybridization on sll germline clone ( glc ) embryo using wg antisense probe showed defective en protein bands and defective wg transcripts bands

    通過對sll生殖系克隆胚胎進行的抗engrailed （ en ）抗體染色和wg反義探針的原位雜交結果表明，與野生型果蠅的胚胎相比較， en蛋白和wg轉錄物條帶出現了明顯的缺失。
  14. Anti - en antibody staining and in situ hybridization on oxt glc embryo using wg antisense probe showed defective en protein and defective wg transcripts bands. the embryonic analysis results indicated that the disruption of oxt gene would lead to the disruption of wg transcription and the expression of engrailed gene, as the wg transcription is dependant on the engrailed

    通過對oxt生殖系克隆胚胎進行的抗en抗體染色和wg反義探針a雜交結果表明，與野生型果蠅的胚胎相比， en蛋白和wg轉錄物條帶都出現了明顯的缺失。
  15. The total number of r - bands found in the chromosomes is 100, which is approximately 1 / 4 to 1 / 5 of that of the vertebrate metaphase chromosome r - bands. they consist of 57 positive, 33 negative and 10 variable bands

    在100條r帶中陽性帶有57個，陰性帶有33個，可變帶有10個，約有722 %的染色體區域顯示r帶陽性結果。
  16. The results showed that pcr applify a 485 specific molecular band in 672 individuals, the rate of positive reaction is 60 %, southern blot result shows a strong signal in transgenic fish. it is concluded that hu - - ifn gene has been integrated and expression. it is also that foreign gene integrating position and copy number is different individuals, by elis a detecting, 23. 5 % transgenic fish " were detected hu - - ifn gene expression in all 672 transgenic fish, but expression level of hu - - dfn is significant difference in different individuals and growth period

    2000年共顯微注射32197粒草魚受精卵，出苗12945尾草魚魚苗，經孵化培育最後獲得1120尾五寸左右草魚，對1120尾草魚提取血液總dna進行pcr和southern雜交檢測， 2000年轉導的草魚的pcr檢測結果，有672尾草魚即600k轉化個體的總dna能擴增出一條485kb的特異分子帶，說明轉化個體整合有huj ifn基因，經southern雜交進一步證實，轉入的抗病相關基因己在轉基因草魚體內染色體上得以整合，但整合位點沒有固定區域，整合的拷貝數存在較大差異。
  17. 3. r - banding the rhg banding ( r - banding ) technique used was the method of dutrillaux and lejeune ( 1971 ) slightly modified

    R帶型青島文呂魚染色體的核型及帶型研究參照nutrillaux和lejeune （ 1971 ）的r帶顯示方法，稍作修改。
  18. Additionally, hau3r gene with it own promoter was cloned into high - copy plasmid pij653 and integrative plasmid pset152, respectively. transformants of s. lividans zx1 carrying these clones were infected with hau3, respectively, but the results shown that there was no significant correlation between the copy number of hau3r gene and the level of resistance to hau3

    另外，將染色體來源的攜帶自身啟動子的hau3 ~ r基因分別克隆到高拷貝和低拷貝載體上並將其導入變鉛青鏈黴菌zx1 ，在實驗條件下未發現拷貝數與抗性水平間存在顯著相關性。
  19. The positive transformants with the integrates mn - sod gene and cuzn - sod gene were identified by zeocin - resistance, pcr screening and expression in p. pastoris. the recombinant mn - sod protein and cuzn - sod protein was successfully expressed in pichia pastoris based on the evidences that the obvious activity of sod existed in native - page and enzymatic activity test

    Pcr鑒定進一步說明，目標基因已經重組到宿主基因組染色體上； 0 . 5甲醇誘導表達后，活性電泳出現明顯活性條帶，重組酵母發酵液中mn - sod的活性約是對照菌株sod活性的2 . 6倍； cuzn - sod酶活力約是對照菌株sod活性的1 . 3倍。
  20. The positive transformants with the integrates mn - sod gene was identified by zeocin - resistance, pcr screening and expression in p. pastoris. the recombinant mn - sod protein was successfully expressed in pichia pastoris based on the evidences that a relative molecular weight about 23kd appeard in sds - page, the obvious activity of sod existed in native - page and enzymatic activity test, and mn - sod activity was specific base on the inhibition with the mixture of chloroform - enthanol ( 3 : 5 / v : v ) and potassium cyanide. two secreted plasmids ppiczaa - sodm18 and ppiczaa - sodc were constructed and after there linearization were transferred into chromosome of pichia pastoris gs115 by electroporation

    Pcr鑒定及mut表型分析進一步說明，目標基因已經重組到宿主菌基因組染色體上； 0 . 5甲醇誘導表達后， sds - page結果顯示，表達的蛋白相對分子量約為23kd ，活性電泳出現明顯活性條帶；酶活性測定顯示，重組菌株sod活性比對照提高5倍左右；氯仿-乙醇（ 3 : 5 v : v ）和kcn （ 5mmol l ）抑制反應進一步證明，所表達的sod為錳超氧化物歧化酶。
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