魚子孵化 的英文怎麼說
中文拼音 [yúzifūhuà]
魚子孵化
英文
fsh hatching-
With the cold shock, the diploid eggs also developed to diploid gynogens after the activation of the uv - irradiated sperm, no tetraploid gynogens being found, suggesting that it is difficult to double the chromosome from the diploid to tetraploid by means of cold shock treatment
沒有檢測到雌核發育四倍體。這表明通過冷休克讓二倍體卵子加倍成四倍體比較困難，但是經冷休克處理獲得的雌核發育魚的孵化率高於沒有進行冷休克處理的。The optimum parameters, such as the exact time for the activated eggs finishing the first chromosome sets copying, the optimum period for artificial chromosome set duplication, the optimum temperature and intensity of heat shocking etc, were determined
確定了在一定孵化溫度下，被激活的草魚卵子完成第一次染色體復制的準確時間，實施人工染色體加倍處理的最佳時機，人工加倍處理的最佳方法和處理強度等。The results showed that pcr applify a 485 specific molecular band in 672 individuals, the rate of positive reaction is 60 %, southern blot result shows a strong signal in transgenic fish. it is concluded that hu - - ifn gene has been integrated and expression. it is also that foreign gene integrating position and copy number is different individuals, by elis a detecting, 23. 5 % transgenic fish " were detected hu - - ifn gene expression in all 672 transgenic fish, but expression level of hu - - dfn is significant difference in different individuals and growth period
2000年共顯微注射32197粒草魚受精卵，出苗12945尾草魚魚苗，經孵化培育最後獲得1120尾五寸左右草魚，對1120尾草魚提取血液總dna進行pcr和southern雜交檢測， 2000年轉導的草魚的pcr檢測結果，有672尾草魚即600k轉化個體的總dna能擴增出一條485kb的特異分子帶，說明轉化個體整合有huj ifn基因，經southern雜交進一步證實，轉入的抗病相關基因己在轉基因草魚體內染色體上得以整合，但整合位點沒有固定區域，整合的拷貝數存在較大差異。
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