鯊烯合酶 的英文怎麼說
中文拼音 [shāxīgě]
鯊烯合酶
英文
squalene synthase-
For the first time a 1539bp squalene synthase ( asqs ) cdna was cloned from a high - yield artemisia annua strain 001 by race strategy
用race方法首次從青蒿中克隆了一個1539bp全長鯊烯合酶cdna 。Asqs is 70 %, 77 %, 44 % and 39 % identical to squalene synthases from arabidopsis thaliana, tobacco, human and yeast, respectively. the asqs genomic dna has a complex organization containing 14 exons and 13 introns
青蒿鯊烯合酶氨基酸序列與擬南芥、煙草、人類、酵母鯊烯合酶的一致性分別為70 、 77 、 44 、 39 。Full - length or truncated cdna was subcloned into prokaryotic expression vector pet30a and expression induced in e. coli bl21 ( de3 ). no squalene synthase polypeptide of expected molecular mass was observed in e. coli containing the putative full - length squalene synthase cdna, however, overexpression in e. coli was achieved by truncating 30 hydrophobic amino acids at the carboxy terminus
但在含有全長的鯊烯合酶cdna的大腸桿菌中並沒有觀察到預期大小的鯊烯合酶表達,而c末端截短30個疏水氨基酸的鯊烯合酶可在大腸桿菌中過量表達。
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