鹽基替換 的英文怎麼說
中文拼音 [yánjītìhuàn]
鹽基替換
英文
base substitution-
After absorption, the all - frans - retinal isomerizes to a 13 - c / s configuration and br undergoes a photocycle : br570 k590 l550 m410 n520 o640 br570 bacteriohodopsin is a promising biological photoelectric material. we intent to conduct a more thermostable br by site - directed mutagensis. a point ( no. 274 t, no. 274 a turn to c g ) mutation was introduced to br gene by successive pcr technique, and cloned into puc - 19 vector
本論文利用連續pcr的方法定點突變了br基因的一個氨基酸,突變位點選擇了br基因第273的t和274位的a ,把它們變為cg ( tyr替換為arg ) ,然後把突變的br基因克隆入嗜鹽菌表達載體pnov - r ,經測序鑒定的表達載體轉化br缺陷的嗜鹽菌,構建了tyr79 argbr突變體。
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