黴菌固醇 的英文怎麼說
中文拼音 [méijūngùchún]
黴菌固醇
英文
mycosterol-
The nucleotide ( nt ) sequence of the insert in phz1754 is 2299bps in size. computer assisted analysis of the sequence revealed an open reading frame ( orf ) with a g + c content of 70. 3 % that would encode a protein of 552 amino acids ( aa ). the nt seque nce comparision revealed that the orf in the sequenced region exhibits 85 % dna sequence homology with the cholesterol oxidase gene choa of streptomyces sp
對phz1754進行外切核酸酶（ exonuclease ， exo ）順序缺失，獲得單向長度漸減重疊的系列突變體，核苷酸序列測定顯示出該ecor - sal片段的精確大小為2299bps ， frameplot程序分析揭示出該區域一個完整的開放閱讀框（ orf ）的存在，其大小為1656bps ， g + c含量為70 . 3 ，編碼552個氨基酸，利用blastsearch程序將orf的核苷酸序列及推導的氨基酸序列與因特網上基因及蛋白質數據庫進行綜合比較，發現無論在核苷酸水平還是在蛋白水平上，該orf均與膽固醇氧化酶表現出同源性，而且與鏈黴菌膽固醇氧化酶同源性最高，說明該orf編碼膽固醇氧化酶基因。Strain sa - coo by southern hybridization. a cosmid - based gene library of streptomyces griseus atcc14811 was constructed using phz1357, a streptomyces - e. coli bifunctional vector carrying two cohesive sites
為了獲得膽固醇氧化酶基因，以大腸桿菌-鏈黴菌雙功能柯斯質粒phz1357為載體，構建了灰色鏈黴菌atcc14811的基因組文庫。The enzyme retained full activity after being treated at room temperature for 1 hour at ph between 4. 0 and 11. 5. the enzyme can be incubated at 50 for 4h with only less 50 percent loss of activity and is stable in the frozen state. when streptomyces griseus atcc14811 was cultured in 10. 3 % sucrose yeme liquid medium, production of extracellular cholesterol oxidase increased for 5 days before decrease
利用硫酸銨鹽析及deae -纖維素離子交換柱層析提取純化灰色鏈黴菌atcc14811發酵上清液中的膽固醇氧化酶，理化性質研究表明酶作用晟適ph為8 . 0 ，最適溫度為45 ， ph穩定范圍在ph4 . 0 - 11 . 5之間，在50條件下保溫4h ，仍保留54酶活力。Air of this advanced development provides efficient methods for protein engineering. in this study, a new two - step procedure has been developed for the detection of cholesterol oxidase activity in streptomyces strains. streptomyces griseus atcc14811 was confirmed to produce cholesterol oxidase and it also showed homologies with the cholesterol oxidase gene ( choa ) from streptomyces sp
本實驗設計了簡單易行的鏈黴菌膽固醇氧化酶活性測定指示平板，結合用鏈黴菌sa - coo （ streptomycessp . strainsa - coo ）膽固醇氧化酶基因（ choa ）為異源探針的southern雜交，確證了灰色鏈黴菌atcc14811 （ streptomycesgriseusatcc14811 ）具備產生膽固醇氧化酶的能力。分享友人