黴菌活性 的英文怎麼說
中文拼音 [méijūnhuóxìng]
黴菌活性
英文
fungal activity- 菌 : 菌名詞1. (蕈) mushroom2. (姓氏) a surname
- 活 : Ⅰ動詞1 (生存; 有生命) live 2 [書面語](救活) save (the life of a person):活人無算 (of a goo...
- 性 : Ⅰ名詞1 (性格) nature; character; disposition 2 (性能; 性質) property; quality 3 (性別) sex ...
- 黴菌 : [微生物學] mould; mycete; mucedine
- 活性 : [化學] activity; active; activated活性肥料 active fertilizer; 活性酵母 active dry yeast; 活性粘土...
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The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中,通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中,然後採用葉盤法,在該農桿菌的介導下將pap基因導入普通煙草中,經過卡那黴素抗性篩選,最後獲得了轉pap基因的工程煙草植株,摩擦接種煙草花葉病毒( tmv ) ,與非轉基因煙草相比,能夠推遲癥狀表現達2月之久,說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。Arginine feeding experiment showed that nitrogen metabolism in the s. tenebraius was obviously affected by arginine through two possible ways : ( l ) pronase activity in vitro could be influnced by arginine, as a result, the catabolism of nitrogen - containing macro - molecule was promoted and the nitrogen element in the broth was increased. ( 2 ) arginine could be transformed into glutamic acid, so that the biosynthesis of apramycin was promoted
因而我們認為gln可能是安普黴素生物合成氮元素的供體。 arg添加實驗結果表明, arg可能通過兩種途徑影響黑暗鏈黴菌體內的氮代謝: ( 1 ) arg可能影響胞外蛋白酶的活性,進而促進含氮大分子物質的分解代謝,補充發酵過程中的氮素來源。Synthesis and antibacterial activities of erythromycin derivatives
紅黴素衍生物的合成及抗菌活性The results showed that the relative survival rate of zoospores decreased with the uv radiation time and distance increased, and the uv radiation could reduce the growth rate, survival capacity, pathogenicity and reproduction capacity of phythophthora sojae remarkably
結果表明:游動孢子的相對存活率隨照射距離及時間的延長而降低,紫外線照射使大豆疫黴菌生長速率、生存能力、生殖能力、致病性等都明顯降低。The results showed that the extract by etoac presented a significant effect on staphylococcus aureau 、 bacillus cereus 、 bacillus megateriurn 、 proteus species, corynebacterium pekinense 、 trichoderma viride and aspergillus flavus all of which belonged to bacteria ; the extract by n - buoh presented significant inhibitory effect on bacillus mesentericus, bacillus subtilis 、 proteus species 、 bacterium prodigious, trichoderma viride and aspergillus flavus all of which belonged to bacterium ; the inhibitory effect become stronger and stronger with the extract concentration increaseing and the water phase of the extract did not present any antimicrobial effects
結果表明,乙酸乙酯萃取物對細菌中的金黃色葡萄球菌、蠟狀芽孢桿菌、變形桿菌、巨大芽孢桿菌、北京棒狀桿菌和黴菌中的綠色木霉、黃麴黴有明顯的抑菌作用;正丁醇萃取物對細菌中的馬鈴薯芽孢桿菌、變形桿菌、枯草芽孢桿菌、靈桿菌和黴菌中的綠色木霉以及黃麴黴有明顯的抑菌作用,且提取物的抑菌作用隨濃度增大而增強,而水相則沒有抑菌活性。In this study, the avermectin - producing strain streptomyces avermitilis was studied and the avermectin biosynthesis gene cluster in the genomic dna of streptomyces avermitilis s - 2 was altered by the method of gene engineering. insertion inactivation of aved gene in the cluster by introducing apramycin resistance gene into aved gene resulted in the disappearance of " a " components of avermectins. when avec gene was inactivated by the same way, four " 1 " components were lost and only " 2 " components, the potential precursor of ivermectin, were accumulated
將該基因簇中的aved基因通過插入外源的安普黴素抗性基因片段使其失活,導致發酵產物中4個a組分(不需要的組分)的消失;將基因簇中的avec基因通過同樣手段,使其失活,導致發酵產物中4個「 1 」組分的消失,而主要積累「 2 」組分(進一步改造可成為伊維菌素的前體b _ 2組分) 。An evaluation of the bacteriostatic activity of tobramycin - impregnated selfsetting cpc in vitro
載藥妥布黴素體外抗菌活性評價Inhibition of botrytis cinerea by wuyiencin and variation of enzymic activities associated with disease resistance in tomato
武夷菌素對番茄灰黴菌的抑制作用及對番茄抗病性相關酶活性的影響The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides
進一步對xynba進行了脫糖基化處理得到xynbb ,其分子量恢復到23kd ,證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現:三種酶的最適ph差異不大, xynb和xynba均為5 . 2 , xynbb為5 . 0 ; xynb和xynba的最適溫度均為60 , xynbb降為50 :在耐熱性上, xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ; xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ,明顯低於原酶的比活2814 . 45iu mg ; xynb和xynba的km值相當,分別為21 . 56 ( g kg )和20 . 87 ( g kg ) ,而xynbb的km值較大為27 . 10 ( g kg ) ; xynba和xynbb的vmax相差不大,分別為4568 mol mg ? min和5329 mol mg ? min ,明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性,對胃蛋白酶和胰蛋白酶有很好的抗性,且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現:以樺木木聚糖為底物時,酶解產物主要為木三糖和木四糖,含量分別為68 . 43和16 . 50 ,另外還含有11 . 79的木二糖;以玉米芯木聚糖為底物時,酶解產物主要為木二糖和木三糖,含量分別為81 . 78和11 . 55 。It is studied that the activity against eumycetes of the fermentation filtrate ( 16h ) of strain jw - 725 is the strongest. the optimum nutritional sources are wheat bran and soya bean meal. the fermentation filtrate reserved under 4 still has bioactivity after 20 days or so, but its bioactivity decreases much while being treated under high temperature. the ph of fermentation filtrate has much influence upon the bioactive substance, and it remains activity at the range of 5
發酵液在4下保存20天左右仍有抗菌活性;但經高溫處理后,其抗菌活性降低很多; ph對抑菌物質的生物活性影響比較大,抑菌物質保持活性的ph范圍是5 . 0 7 . 0 。分離到的多粘芽孢桿菌( b polymyxajw - 725 )對柑橘青黴菌( pIn this study, we attempted to construct an engineering strain producing only avermectin bl through the replacement of dna encoding dh2 - kr2 domains of the avermectin pks ( avedh2 - kr2 ) with dna encoding dh2 - kr2 domains from the pikromycin pks in s. avermitilis olm73 - 12, producing only avermectins b and no oligomycin. gene replacement vector pxl201 ( pkc1139 : : 5 ' flank + pia : dh2 - kr2 + 3 ' flank ) was used to transform 5. avermitilis olm73 - 12 protoplasts
我們以不產寡黴素而僅產阿維菌素b的工程菌olm73 - 12為出發菌株,用委內瑞拉鏈黴菌( streptomycesvenezuelae )中編碼pikromycinpks模塊2上完全活性的dh和酮基還原酶( kr )的dna區域對olm73 - 12染色體上編碼阿維菌素pks模塊2中dh和kr的區域進行取代,試圖構建僅產b1組分的基因工程菌。Based on a 3. 1kb pst i fragment of genomic dna of a wild s. avermitilis, a 1. 5 kb apramycin resistance fragment was inserted into sph i site of avec gene in the 3. 1 kb fragment, then a recombinant plasmid pc05 was obtained by introducing above inactivated avec fragment into mcs region of phjl401. competent cells of et12567 were transformed by recombinant plasmid pid03 and pc05 respectively
以含有avec基因的3 . 1kb基因組dnapsti片段為基礎,將1 . 5kb的安普黴素抗性基因片段插入到avec基因中的sphi酶切位點,再將此插入失活的avec基因片段連接到具有接合轉移功能(含有orit基因)的鏈黴菌-大腸桿菌穿梭質粒phjl401的多克隆位點區,由此得到重組質粒pc05 。The minimum inhibitory concentrations ( mic ) of imipenem, panipenem and meropenem for 225 clinical isolates was determined by agar dilution method, in comparison to 13 other antimicrobial agents
結果,三種碳青黴烯類抗生素對腸桿菌科細菌具高度抗菌活性,對銅綠假單胞菌、不動桿菌屬、糞腸球菌等亦具良好抗菌作用。In vitro antibacterial activity of fosfomycin in combination with 14 antimicrobial agents
磷黴素與14種抗菌藥物分別聯用的體外抗菌活性研究16s rdna sequence analysis of three strains of 050642, 060386 and 060524 whose id50 is above 1200 indicated they also belong to streptomyces genus and strain 050642 is suspected to be a novel streptomyces. spp with the highest similarity to streptomyces colicolor, at 95 %
對三株有較強活性的菌株050642 、 060386和060524進行了16srdna序列分析,初步確定這三株菌都屬鏈黴菌屬,其中菌株050642與其親緣關系最近菌株的相似性僅為95 ,極有可能是鏈黴菌屬的一個新種。In 52 strains filamentous fungi and 82 strains macrofungi, the percentages of antitumor strains are as follows : 15. 4 % filamentous fungi, 21. 9 % macrofungi, most of which belong to aspergillus sp
52株絲狀真菌和82種大型真菌的生物活性測定結果顯示,絲狀真菌中的15 4和大型真菌的21 9具有抗腫瘤活性,主要分佈於麴黴屬中和傘菌目中。A 1. 5 kb apramycin resistance fragment was inserted into nru i site of aved gene and the inactivated aved gene fragment was then introduced into mcs region of phjl401 - an e. coli / streptomyces shuttle vector with conjugation function ( containing orit gene ). as a result of above procedures, a recombinant plasmid pid03 was obtained
將1 . 5kb的安普黴素抗性基因片段插入到aved基因中的nrui酶切位點,再將此滅活的aved基因片段插入到具有接合轉移功能(含有orit基因)的鏈黴菌?大腸桿菌穿梭質粒phjl401的多克隆位點區,由此得到重組質粒pid03 。In order to increase the products yields from g lucidum and m. purpureus and improve their production level, these fungus were studied deeply in this paper
為了拓展這兩種真菌的生物活性產品種類及提高其生產水平,我們對靈芝和紅麴黴進行了較為深入的研究。Also the ci represser was expressed, presumably from its own phage promoter and prevented transcription from pr at low temperature. moreover, s. lividans strains expressing the c. 100 kda pks were different in sporulation and antibiotic production compared to strains without the 2. 7 kb pks gene, suggesting that the pks protein was active in an unknown manner in streptomyces
表達pks的變鉛青鏈黴菌菌株在孢子形成和抗生素產生方面與沒有2 . 7kbpks基因的菌株相比較是有區別的,因而推測在鏈黴菌中表達的雙功能結構域pks蛋白具有活性。The study on the anti - bacteria effects of azithromycin sodium dihydrogen phosphate in vitro
阿齊黴素磷酸二氫鈉體外抗菌活性的研究分享友人