By the compounds of submandibular gland cells and collagen sponges. we investigate the optimal cell denisity of tissue engineered compound of submandibular gland cells and collagen sponges, the cellular compatibility of tissue engineered compound of submandibular gland cells on the collagen sponges with different porosity and the influence of epidermal growth factor on the adherence of submandibular gland cell to collagen sponge. our studies can primary provide theoretical ground work to form the model in vitro of tissue engineering smg
在本研究中,以初步探討體外頜下腺細胞與膠原海綿支架相互作用為目的,採用體外分離培養sd大
鼠頜下腺細胞,然後接種于膠原海綿支架上體外復合培養的方法;從不同接種細胞濃度對細胞一支架復合物影響,同一接種細胞濃度在不同
孔隙率的支架上黏附、增殖的情況及表皮生長因子( egf )對頜下腺細胞的促增殖作用,促細胞在支架上黏附等三方面入手,初步研究了頜下腺細胞與膠原海綿相互作用的影響因素,為進一步在體外及體內構建較為理想的組織工程化頜下腺提供理論參數和實驗依據。
In vitro injury models of brain slice ( ogd and nmda insult ) and primary neuronal cultures ( nmda insult ) oxygen / glucose deprivation ( ogd ) - induced injury of rat hippocampal slice in vitro the rat hippocampal slices prepared were allowed to recover in the normal artificial cerebrospinal fluid ( acsf ) bubbled with gas mixture of 95 % o2 + 5 % co2 for 1 h, then they were thansfered to glucose - free nacsf which was bubbled with gas mixture of 95 % n2 + 5 % co2. after treatment with ogd, the slices were placed into 2 % ttc solution in dark and incubated at 37 * cfor 1h. the slices were weighted and a 50 : 50 mixture of ethanol / dimethyl sulfoxide was then added to extract the formazan in dark for 24 h
離體腦片損傷模型( ogd和nmda )及原代培養神經元nmda損傷大
鼠離體海馬腦片缺氧缺糖( ogd )損傷大
鼠離體海馬腦片制備后,在通氧混合氣的正常腦脊液( nacsf )中恢復60min ,然後移入通氮混合氣的無糖腦脊液(吵化sf )中缺氧缺糖,取出腦片與2 ttc避光37 』 c溫浴60dll ,染色后根據每克濕重加入20ml抽提液(乙醇:二甲亞礬一50 : 50 ) ,在密閉容器內避光置24h ,測量前搖勻后取200pi至96
孔板,在490urn波長,酶標儀測定各
孔od值。
In the present study, using the conventional whole - cell mode and perforated whole - cell patch claxnp mode, the role of intracellular and extrce1lular calcium in snp - induced increase of ik (. ) were investigated. l
我們分別應用傳統全細胞模式和穿
孔模式的膜片鉗技術,研究了細胞內外鈣離于在s p誘導人h )增加中的作用,進而闡明no引起豚
鼠胃竇環行肌舒張的離子機制。
Using the eye - gaze distinguishing and tracking method based on the image processing technique, the position relationship between pupil center and glint which produced by the corneal reflection and the near - infrared light was analyzed, the fixation point of the eye - gaze on display screen was deduced, and the mouse accuracy by eye - controlled in the screen may achieve to 40 pixels
摘要利用基於圖像處理技術的視線方向識別、跟蹤方法,通過近紅外光線在眼睛角膜反射產生的光斑和瞳
孔中心的位置關系來確定視線方向,推導出人眼在計算機屏幕上的注視點,進而實現人眼對
鼠標的定位與操作,其在顯示屏上定位精度可達到40 40像素。
After 15h of maturation. 98 % of oocytes released the first polar body and thus the first meiosis of mouse oocytes ended. secondly, strong chromosome to chromosome binding was induced by culturing pro - mi oocytes in demecolcine supplemented medium
第一次減數分裂期小
鼠卵母細胞經化學去核后,去除透明帶,並胎兒成纖維細胞粘合、電融合併srcl2激活,培養于微
孔中。
Another scientist cured type 1 diabetes in rats with a nanoengineered device that uses seven - nanometer pores to controllably release insulin while blocking antibodies
另一位科學家利用奈米設計的裝置,在大
鼠身上治愈了第一型糖尿病;該裝置具有七奈米大小的
孔洞,可調節胰島素的釋放,還可阻斷抗體的侵犯。
The peacocks strolling in the woods, the squirrels jumping on the trees, the pigeons gathering on the lawn, all of them manifest the true harmony between human and the nature
您可以碰到在林中漫步的孔雀,在樹上跳躍的松鼠,在草地上聚集的鴿子。