鼠血清白蛋白 的英文怎麼說
中文拼音 [shǔxiěqīngbáidànbái]
鼠血清白蛋白
英文
mouse serum albumin- 鼠 : 名詞(老鼠) mouse; rat
- 血 : 血名詞(血液 多用於口語) blood:吐血 spit (up) blood; 血的教訓 a lesson paid for [written] in b...
- 清 : Ⅰ形容詞1 (純凈) unmixed; clear 2 (寂靜) quiet 3 (清楚) distinct; clarified 4 (一點不留) w...
- 白 : Ⅰ形容詞1 (似雪的顏色) white 2 (清楚; 明白; 弄明白) clear 3 (空的; 沒加他物的) pure; clear; ...
- 蛋 : 名詞1. (鳥類或龜、蛇類所產的卵) egg 2. (像蛋形的東西) an egg-shaped thing 3. (辱罵之詞)
- 清白 : pure; clean; unsullied; have clean hands; stainless
- 蛋白 : 1. (卵中透明的膠狀物質) egg white; albumen; gary2. [生物化學] (蛋白質) protein
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Abstract : the anti - inflammatory effect of n - butanol extract of xanthocerassorbifolia bunge ( bex ) was studied in this paper. the ear edema resulted from dimethyl benzene, paw edema induced by carrageenin, the increase of vascular permeability caused by acetic acid, the chemotaxis of wbc induced by cmc and the weight of cotton granuloma in mice and hind paw edema induced by fresh egg white in rats were inhibited significantly by ig bex daily for 7 days, respectively. the carrageenin - induced paw edema was also inhibited markedly in adrenal - ectomiced mice. no changes in weight of adrenal and the concentration of vitamin c were observed, indicating that the anti - inflammatory effect of bex was not related to pituitary - adrenal system
文摘:文冠木正丁醇提取物對二甲苯致小鼠耳腫脹、蛋清致大鼠足腫脹、角叉菜膠致小鼠足腫脹、醋酸致小鼠腹腔毛細血管通透性增加、小鼠羧甲基纖維素囊中白細胞遊走、小鼠棉球肉芽腫生長均有顯著的抑制作用,同樣劑量下,文冠木正丁醇提取物對角叉菜膠致去雙側腎上腺小鼠足腫脹仍有顯著的抑制作用,且對小鼠腎上腺重量及腎上腺中維生素c的含量沒有明顯影響。This modification includes : ( 1 ) selecting two important molecules as candidates, ( 2 ) choosing a promiscuous t - cell epitope, and two b - cell epitopes or conserved amino acid sequences from the two important molecules, ( 3 ) connecting them adequately through analysis by the molecule designing software. therefore, the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing. based on the molecule designing methods, the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse, as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected
本研究以蛋白質分子設計的理論和方法研究避孕疫苗,將sp17和cyritestin關鍵表位和牛核糖核酸酶非選擇性th細胞表位合理組合,獲得新抗原- 35肽序列;並在合成、純化後分別與弗氏佐劑、免疫刺激復合物( iscoms )混合后免疫不同遺傳背景的雌性小鼠,觀察血清和生殖道內的特異性抗體滴度的動態變化、生育力的改變以及免疫后小鼠重要臟器的組織病理學改變:以及在ivf下,新抗原的特異性抗血清對精卵相互作用的影響及抗原在精子表面的特異性定位。Anti - melatonin monoclonal antibodies of higher titer, affinity and good sensitivity were obtained by coupling mt to bovine serum albumin with formaldehyde and by immunizing mice with multifocal intra - dermal injections. we obtained 6 strains of hybridoma, all of them secreting specific antibodies to mt, we apply antibodies to determinate free mt inhuman serun with group - selective immunoassay technique. an inhibition curve for mt was obtained in the range of 50pg to30ng, and 1. 4ng of mt inhibited the value of the assay by half. we evaluate the specificity of antibodies by determination of cross - reactivity of several analogues, the moabs recognized mt but
通過將mt用甲醛作連結劑連結到牛血清白蛋白上sa採用皮下多點注射兔疫小鼠得到了高效價,高親和力,較好特異性的抗mt單克隆抗體,最後獲得了5株單克隆細胞株,都能分泌針對mt的特異性抗體,建立了選擇性基團免疫分析法,用制備的抗體測定了人血清中mt的含量,作了mt的抑制標準曲線,其抑制范圍從50pg ? 30ng ,半抑制量為1In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company
實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 ( 1 : 1 )混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑( upofectalnineplusreageni )和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 ( do一7 )蛋白(即用型) ;鼠單克隆抗體p21waf , (即用型) ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰(濃縮型) ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。Methods 50 wistar rats were sensitized by ovalbumin as animal model of ar and randomly divided into 5 groups : model group, ketotifen fumarate group, xinqin granules group, low - dose minkang tablet group, and high - dose minkang tablet group, another 10 healthy rats in normal group
方法除正常組外,其餘大鼠用卵清蛋白造成變應性鼻炎模型,隨機分為模型組、西藥對照組(酮替芬組) 、中藥對照組(辛芩沖劑組) 、敏康片小劑量組、敏康片大劑量組;用藥1周后觀察大鼠一般情況及鼻黏膜肥大細胞數、脫顆粒細胞數、血漿組胺的變化。( 2 ) mice ' s activity of cu, zn - sod and cp were not affected significantly by dietary cu source, mice ' s activity of cu, zn - sod and cp increased by dietary supplemental copper
( 2 )飼糧銅源對小鼠血清銅鋅超氧化物歧化酶活性和血清銅藍蛋白活性沒有顯著影響。Following the 7d, 14d, 21d, 28d feeding period, in each treatment, one mouse was killed to count the ratio of peripheral anae + t lymphocyte, serum igg content, serum cu, zn - sod activity, serum cp activity, immune organ index, copper concentration in liver and spleen
在正式試驗期第7天、 14天、 21天、 28天,每重復選取1隻小鼠,測定外周血t淋巴細胞百分比、血清igg含量、血清銅鋅超氧化物歧化酶和銅藍蛋白活性、免疫器官指數及肝臟和脾臟銅含量。The surviving time upon heat exposure and the swimming time were examined and the physiologic and biochemical changes such as body weight, potassium concentration, blood sugar, hemoglobin were compared before and after heat exposure
並比較了高溫適應前後以及高溫處理前後小鼠的一些生理生化指標如體重、血清中鉀離子濃度、血糖含量和血紅蛋白含量。Correct clones were selected and plasmid dna was isolated and digested with saci and puvii. a dna fragment of about 2. 1kb was purified and labeled by dig - 11dutp as probe. at least 40 positive clones were obtained from human genomic library by in situ colony hybridyzation with this probe. among them one clone contains human serum album dna by sequence
以pcr擴增的人血清白蛋白( hsa )基因片段為探針,從人的基因組文庫中雜交篩選的陽性克隆中,經測序分析,有一個克隆含有全長hsadna ,用從其它的陽性克隆中選取兩種dna片段,即dna修復基因hfen1和一段非編碼大片段cit987sk - 384d8 ,與人hsadna一起,進行顯微共注射,成功制備了轉多基因小鼠。Protective effects of eleutherosides acupoint application on rats with sleep deprivation
睡眠剝奪對大鼠血清髓鞘堿性蛋白和海馬超微結構的影響2. the putative epitopes that displayed on phages were identified by indirect elisa using swine antisera against prrsv and mouse antisera to recombinant structural protein of prrsv. seven putative epitopes could be recognized by antisera
利用prrsvbj - 4陽性血清和鼠源抗重組結構蛋白抗體,採用間接elisa方法對噬菌體展示的表位進行了鑒定。Then protein crystal of eif - 5a was observed using atomic force microscopy. further, the polyclonal and monoclonal antibodies were gained from immunized rabbits and rats. hypusine - contained eif - 5a plays a role in cell growth and differentiation
用得到的重組蛋白分別免疫家兔和小鼠,獲得兔的抗血清和三株持續分泌單抗的雜交瘤細胞株,為進一步分析eif - 5a的功能提供了檢測手段。These indicated that the porcine il - 6 gene was correctly transcribed and translated in the recombinant bacteria. the fusion protein was recovered from polyacrylamid gel and used to immunize the rabbit, and the liter of rabbit anti - porcine il - 6 serum from immunized rabbit was 1 : 128, 000
從制備性聚丙烯酰胺凝膠中回收融合蛋白,用mtt法測定重組蛋白刺激小鼠脾淋巴母細胞增殖反應活性,並以重組蛋白免疫家兔制備抗豬il - 6滴度為128 , 000的高免兔血清。The differential screening of serum proteins in diet - induced obese and obese - resistant rats
飲食誘導肥胖與肥胖抵抗大鼠血清差異蛋白篩選分享友人