activity staining 中文意思是什麼
activity staining
解釋
然後採用平板初熏再配合活性染色法-
( 3 ) isolation and culture of human primordial germ cells ( pgcs ). human pgcs collected from gonadal ridges and mesenteries were grown on mouse feeder layers in the presence of human recombinant leukemia inhibitory factor ( lif ), human recombinant basic fibroblast growth factor, and forskolin as described previously. initially, pgcs were visualized by alkaline phosphatase activity staining
（ 3 ）人類pgcs的分離和培養從4 10周齡藥物流產胚胎的生殖嵴和腸系膜組織中分離原始生殖細胞（ primordialgermcells ， pgcs ） ，培養在添加人重組白血病抑制因子（ lif ） 、人重組堿性成纖維細胞生長因子（ bfgf ）和forskolin的小鼠飼養層細胞上。 -
In the present study, a compartment cultivation system and histochemical staining were used to investigate the influence of soil available p level, plant p status and soil organic p on the growth and metabolic activity of am fungi. differences in metabolic activity among am fungal isolates and the relationship between metabolic activity and mycorrhizal effectiveness were al so investigated. in addition, am fungi from a wide range of environmental conditions ( originally isolated from north, central and south china ) were used to study the ecological adaptability of am fungi and the influence of edaphic conditions on am fungal growth and metabolic activity
本研究採用分室根箱、組織化學等手段研究了土壤施磷水平、植物磷營養狀況、土壤有機磷對am真菌生長和代謝活性的影響；不同am真菌的代謝活性及其與菌根效應之間的關系，並對我國華北、華中和華南地區篩選出的高效菌株進行了生態適應性的比較，以期在理論上闡述宿主植物生長狀況及土壤條件對菌根真菌生長和代謝活性的調控機制，篩選出具有廣泛生態適應性的am菌株。 -
Histochemical staining in tl transgenic tobaccos showed that sigp1 promoter was not only organ - specific ( especially expressed in leaves and guard cells ), but also highly inducible by multi stress treatment such as drought, aba, high salinity and low temperature. quantitative analysis showed that gus activity in roots was very weak and no great changes were observed after treatment
通過對t1代轉基因苗的組織化學定位表明，該啟動子驅動的gus基因在逆境（如乾旱、 aba 、高鹽和冷）脅迫的葉片中表達，根中不表達，且老葉中的表達量高於幼葉中的表達量，說明其表達受發育階段的調控。 -
With different amount shrnas transfected, in different time, we assayed the telomerase activity of the transfected cells with trap - silver staining and pcr - eia methods
用trap -銀染法和pcr - eia分別檢測經轉染不同shrna量和作用時間hela細胞端粒酶活性。 -
Methods : thirty - two patient samples of primary head and neck squamous cell carcinoma and 15 adjacent tissues were assayed using trap - silver staining for detection of telomerase activity
方法：採用trap銀染法，對32例原發頭頸部鱗癌及15例癌旁組織進行端粒酶活性檢測。 -
Methods : the telonerase activity in 39 endoscopic specimens of colorectal canciomas, 21 endoscopic specimens form ulcerative colitis, 22 from normal colorectal mucosas were examined by silver staining telomerase repeat amplilication protocol ( trap )
方法：應用改良的trap銀染法，檢測39例大腸癌， 21例潰瘍性結腸炎， 22例正常大腸黏膜活性檢標本端粒酶活性。 -
We have also been experimenting with methods for using the exchange of these dyes to visualize synaptic activity, and have recently fostered collaborations to develop new staining molecules for better functional assays
同時我們也完成以交替染料的方式觀察突觸活化的現象，並且在最近和別的實驗室合作研發新的分子染色技術作為更優良的功能性實驗方法。 -
The assay system of the biological activity of lymphotoxin was established using l929 cell as the sensitive target, lt international standard as the positive control and crystal violet staining method to detect viable cell after treated with lt. the best relationship between dosage and effect could be got if the cell seeding density in cell plate was 1. 6 0. 1 104 the dosage of amd was lug / ml, and the starting concentration of dilution in the plate of lt standard was 10 iu / ml with two fold dilution. the credibility of the established system was detected with rhtnfp developed by r & d
為確定經上述步驟純化后得到的目的蛋自lt 27的生物活性，本研究以l929細胞為靶細胞、淋巴毒素國際標準品為參照，採用結晶紫染色法檢測經淋巴毒素處理后存活的細胞，對淋巴毒素生物活性測定的細胞接種濃度、淋巴毒素標準品板上稀釋的起始濃度和梯度稀釋的倍數、放線菌素d的使用劑量等進行條件實驗后，建立了人淋巴毒素生物活性測定方法。 -
2. 4 preparation of yeast telomerase, the activity of telomerase was examined by the method of trap, polyacrylamide gel electrophoresis and silver staining
2 . 4酵母端粒酶的制備，端粒重復擴增法（ trap ） 、聚丙烯酞胺凝膠電泳及硝酸銀染色法檢測端粒酶活性。 -
Methods : in cultured lung explants without serum, the lipid component synthesis of pulmonary surfactant was evaluated in [ 3h ] - choline incorporation ; mrna content of phosphocholine cytidylyltransferase ( cct ) in lung explants was investigated in rt - pcr ; the changes of the ultrastructure of the at ii cells were observed with electron microscope ; the expression of nmdar1 subtype was observed in immunohistochemistry staining ; nitric oxide synthase ( nos ) activity, nitric oxide ( no ) content, superoxide dismutase ( sod ) level, malondialdehyde ( mda ) content and lactae dehydroase ( ldh ) level were determined by biochemistry methods. results : 1. influence of glutamate on synthesis of the lipid component of pulmonary surfactant ? with l - arginine, glu inhibited [ 3h ] - choline incorporation with good dose - dependence and time - dependence ; ( 2 ) mrna content of cct of the glu treatment groups was decreased ; ( 3 ) glu increases the release of ldh in cultured lung explants ; ( dwith electron microscope histochemistry, glu induced the changes of the ultrastruture of at ii iv cells
方法：採用成年大鼠肺組織無血清培養，運用[ ~ 3h ] -膽堿摻入法測定ps主要脂質磷脂酰膽堿（ pc ）合成量； rt - pcr擴增檢測肺組織中pc合成限速酶磷酸膽堿二胞苷酰基轉移酶（ cct ） mrna含量；透射電子顯微鏡法觀察肺泡型上皮細胞和ps系統超微結構的變化；免疫組織化學染色檢測glu的受體nmdar1亞單位的表達；生化測定肺組織乳酸脫氫酶（ ldh ）釋放量和肺組織勻漿中一氧化氮合酶（ nos ）活性、一氧化氮（ no ）生成量、超氧化物歧化酶（ sod ）水平以及丙二醛（ mda ）含量。 -
Cell injury was investigated by lactate dehydrogenase ( ldh ) activity in culture medium. and cell apoptosis was examined by flow cytometry, tunel staining and transmission electron microscopy ( tem )
重度低氧（ 2 3 oz ） 24h ，心肌細胞數和蛋白質合成均降低，並且培養液中ldh水平增高，提示重度低氧有細胞毒性作用。 -
Histochemical gus assay showed that the gus staining was observed only in the mature pollen and germination pollen tube. there is no detectable gus activity in other floral organs, leaves and stems. these results suggested that st901 is a novel pollen - specific gene and the 288bp promoter fragment ( - 297 ? xfrom the translation start codon atg ) is sufficient for pollen - specific expression and os - element regulatory of st901 promoter was possibly concentrated in the region - 297 to - 9
通過對gus酶活性的組織化學定位分析，表明， st901基因啟動子驅動gus基因特異地在成熟花粉和花粉管中表達， st901基因具有花粉表達特性；且288bp （ - 297至- 9 ） （ atg定為+ 1 ）的啟動子區段足以驅動gus基因在花粉中的特異表達， st901基因啟動子的花粉順式表達元件可能位於- 297至- 9之間。 -
Sds - page and activity staining analysis revealed that all of clones 2, 5 and 9 exhibited a single active band with 83 - kd in size, whereas clone 3 exhibited four different bands with 64 kd, 70kd, 76kd and 83 kd after construcing a series of deleted subclones from the plasmid prepared from clone - 5, a complete nucleotide sequences comprising of 3, 205 bps wes determined. one open reading frame ( orf ) was found in this sequence, which was comprised of 2, 661 bps and could encode a polypeptide with 887 amino acid residues
利用基因分析軟體genetyx對clone - 5質粒的核苷酸序列進行分析，並搜索日本dna數據庫（ dnadatabankofjapan ，簡稱ddbj ） ，推斷clone - 5質粒的幾丁質酶基因含有一個2 ， 261bp的開放閱讀框（ orf ） ，編碼一條887個氨基酸的幾丁質酶蛋白； orf上游有一個啟動子。 -
This review focuses on the techniques and methods that are currently used for the detection of apoptosis, such as morphological observation, fluorescence staining, dna ladder, caspase activity, etc, and on the evaluation of the advantages and limitations of these methods
本文綜述了常用的細胞凋亡檢測技術與方法的基本原理，如形態學觀察、熒光素染色、 dna階梯、半胱氨酸天冬氨酸蛋白酶活性的檢測等，並對各種方法的優點及局限性做一簡要比較。
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