aev 中文意思是什麼

aev 解釋
virus]鳥類成紅細胞增多癥病毒
  1. Avian encephalomyelitis virus ( aev ) is a picornavirus with a predilection for the central nervous system and other parenchymous organs of chickens that is transmited by the oral - faecal route. the virus may be spread by the vertical and horizontalroutes, and because of its great stability, contaminated areas may remain infectious for long periods. the egg - adapted van roekel strain is highly neurotropic and does not grow efficiently in the enteric tract of the chicken, and the field isolates of aev is usually enterotropic. despite this. the virion polypeptides of both naturally - occurring strains and the van roekel strain are antigenically identical

    侵害雞的中樞神經系統和其它實質性器官，該病毒通過口-糞途徑傳播，具有水平和垂直傳播的能力。由於它極大的穩定性，被污染的區域可能長期保持傳染性。雞胚適應株vanroekel是高度嗜神經的，並且在雞的腸道內不能有效的生長，而野毒株卻是嗜腸道型的。
  2. Study on elisa method to detect the antibody of aev

    酶聯免疫吸附試驗檢測禽腦脊髓炎抗體方法的研究
  3. The purpose of this study was to learn the sequence and structure of the aev by technology of the molecular biology. to reveal the relation between genome features and function

    本項研究，旨在利用分子生物學的技術手段，搞清楚aev基因組的序列和結構，從而探討基因結構與功能的關系。
  4. Synthesis of igm was restrained after igg was produced, because igm antibodies were producld first in an immune response against aev followed by a switch to igg class

    當igg出現后， igm的合成即受到抑制。抗aev的血清igm和igg在時間上和強度上出現雙峰現象， igm和igg的產生是同一b細胞克隆類型轉換造成的。
  5. Aev has long been recognised as the aetidaogical agent of one of the most significant disease of poultry. despite its importance, relatively little progress had been made towards an understanding of its basic biology and pathogenesis

    Aev一直被認為是最重要的禽病病原之一，盡管它很重要，但有關其基礎生物學，發病機理的了解卻較少。
  6. 10. 14. 25 and 30 days post injection and separated the serum. meanwhile, pathological changes of lymphocytes and changing role of igm, igg from different days old chickens infected with aev - nh937 were systematically studied by viral inoculation of animal, pathological test and elisa

    採用了病毒接種技術、普通病理學方法、酶聯免疫吸附實驗等方法，研究了不同日齡spf雛雞人工感染aev - nh937株后的血清igm 、 igg的動態變化規律。
  7. When artificially and naturally infected, antibody produced is mainly igg, which with high content and long endurence. igg has multiactivities of antibacterial, antivirus, antiexotoxin and so on. it was proved that humoral immunity play important role against the aev - nh937 strain infection at the beginning of the disease

    在人工自動免疫和自然感染后，機體所產生的抗體主要是igg ，它不僅含量多，而且持續時間長，是抗感染免疫的主力，具有抗菌、抗病毒和抗外毒素等多種活性。
  8. Rt - pcr was used to amplify the cdna of the genome. these cdna fragments were cloned into the plasmid pucm - t. the result indicated that the seguence of the genome was obtained. the genome of aev - nh937 composed of 7055 nucleotides, potentially encodes a polyprotein of 2134 amino acids. the genome of aev - nh937 has 94. 3 % nucleotides identity with the calnek vaccine strain of aev

    把它們分段克隆在pucm - t載體上，經序列分析，獲得了aev - nh937毒株的基因組全序列及推導的氨基酸序列，基因組全長為7055個核苷酸，與calnek疫苗株具有94 . 3的同源性，編碼一個含2134個氨基酸的多聚蛋白。
  9. The research consist of four parts. the first part is multiplication, purification and electron microscope examination of the avian encephalomyelitis virus. a 1 : 5 dilution of isolate - nh937 of aev and control group of pbs were inoculated to susceptible 6 - day - old chickens embryos. respectively. after incubation for 10 days, the urinay vesicle liquid was collected. making a comparison the size of the chickens embryos between the test group and the control group, the results showed that the size of the control group is bigger than that of the test group. purified virions were examined under the electron microscope, the result revealed that there are a lot of virions and the aev - nh937 was multiplicated in embryos. the second part was seguence analysis of the genome of the aev - nh937. nine pairs of primers were designed according to published calnek vaccine strain of aev

    本研究共分四個部分：第一部分為aev的增殖，純化和電鏡觀察，用1 : 5倍稀釋的aev - nh937株和陰性對照pbs分別經卵黃囊接種於6dspf雞胚，繼續孵化10d后，收集尿囊液。比較接種組和健康對照組雞胚的大小，結果顯示，健康對照組雞胚明顯大於接種組。分離、提純aev ，把純化的病毒在電鏡下觀察，證明確有大量aev病毒粒子存在，說明aev在雞胚中成功擴增；第二部分是aev - nh937基因組的序列測定工作。
  10. The expressed product was lysised with supersonic wave and purified by sds - page. elisa analysis revealed that the antigenicity of the vpi protein has been detected. the forth part - detection of aev - nh937 strain by in situ hybridization ( 1sh ) - probe was labelled with digoxigenin ( dig ). then the probe hybrid with 5d, 10d, and 20d postinfection brain tissue of chicken. the results of the ish showed that the positive signal was found in 3 cases, while control group was negative. there has been a reasonable correlatien between this method and other detection test

    經超聲波裂解，用尿素溶解包涵體，電泳純化后，利用elisa檢測vp _ 1外殼蛋白，表明具有一定抗原性；第四部分：應用原位雜交檢測aevi用dig標記的探針與sd 、 10d 、 20d的攻毒雞腦組織雜交，來檢測那v的rna 。
  11. In young chickens aev induces paralysis, ataxia and muscular dystrophy, while in older chickens, infection is usually subclinical, resulting in a decline in egg production and hatchability. infectivity was shown to remain unaffected by chloroform, low ph, pepsin, trypsin and deoxyribonuclease. magnesium cations were shown to stabilise preparations of the virus against heat inactivation. the buoyant density of virions are 1. 31g / ml. the diameter of the virion was estimated to be 22 to 30nm. the aev can be adapted to grow in chicken embryo. the inability of aev to grow effeciently in most cell cultures

    幼雞感染該病毒后，引起麻痹、頭頸震顫甚至共濟失調，而成雞常呈亞臨床感染或導致產蛋量和孵化率下降。病毒的感染性不受氯仿、低ph 、胃蛋白酶、胰酶和脫氧核糖核酸酶的影響，鎂離子可增強病毒對熱的穩定性，病毒的浮密度為1 . 31g ml ，直徑為22 - 30nm ，該病毒主要在雞胚中增殖，在大多數細胞培養物中不生長。
  12. The double chains of chil - 1 cdna were labeled with dig - 11 - dutp to produce probe. and the chil - 1 mrna secreation was studied in the tissues of chickens " brains infected with aev and that of normal chickens ". the results showed that the content of chil - 1 secreation in sick chickens " brain was higher than that of the normal chickens "

    用地高辛隨機引物法標記il - 1 cdna制備探針，原位雜交檢測禽腦脊髓炎病雞與正常雞腦組織中il - 1分泌情況，結果表明腦炎病雞腦中il - 1分泌量高於正常雞。
  13. Results showed that igm, after 3 days of inoculation, reached to the highest level ( 0. 57mg / ml ), which indicated some of the plasma cells have produced igm. igg after the 10 day of inoculation, had a trend of increasing and reached to the highest level at 20 th day ( 5. 05 mg / ml ). it indicated that igm, the immune globulin produced from the earlier reaction, play active role against the aev - nh937 strain infection at the beginning of the disease

    實驗結果為igm在攻毒后第3天達到峰值（ 0 . 57mg / ml ） ，說明已有一部分b淋巴細胞分化為漿細胞並產生igm ； igg從第10天開始呈上升趨勢，在第20天達到峰值（ 5 . 05mg ml ） ；感染雞外周血清中先出現igm ，后出現igg ，說明igm為初級反應最早出現的免疫球蛋白，在感染早期起著先鋒免疫作用。
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