aldc 中文意思是什麼
aldc
解釋
技術 自適應無損數據壓縮演算法-
The a - aldc can decarboxylate a - acetolactate directly to form acetoin without the formation of diacetyl
15ppm就會產生類似餿飯的味道。 -
By adding a - aldc to the fermenting wort, a - acetolactate will be removed as soon as it is formed, thereby which can avoid the formation of diacetyl and reduce or even skip the period for maturation
如在啤酒主發酵階段或熟化階段加入- aldc酶制劑，則可大大縮短啤酒熟化期，從而提高設備利用率和節省能源。 -
Aldc information technology - data compression algorithm - adaptive lossless data compression algorithm for information interchange
信息技術.數據壓縮演算法.信息交換自適應無損數據壓縮 -
Approximately 7000 colonies were screened. one mutant was obtained, and its enzyme activity is 2. 09 - fold higher than that of wild - type a - aldc in low temperature. the two nucleotide substitutions were occurred in the evolved a - aldc gene
根據定向進化的基本思想，我們利用易錯pcr的方法，篩選了近7000個菌落，最終得到了一株在低溫下也有較高酶活力的突變體。 -
It clearly allows us to engineer enzymes with novel functions and features by strategies, such as error - prone pcr, dna shuffling and stagger extension process ( step ), when very little is known about spatial configuration of proteins. therefore millions of years in nature can in principle be accomplished in the test within several years. according to the basic idea of directed evolution, we modified the a - aldc gene by error - prone pcr
它不需要事先了解酶的空間結構和催化機制，而通過在體外模擬自然進化機制（隨機突變，重組和自然選擇） ，利用易錯pcr ， dna改組，交錯延伸等方法，改造酶基因，定向選擇（或篩選）有價值的非天然酶，短期內在試管中完成在自然界需要幾百萬年的進化過程。 -
Both the ph stability and thermostabilization of evolved a - aldc were higher than those of wild - type enzyme. this mutant is more suitable for the industrial production of beer. the replacement of arg results in partial disruption of the a - helix
並對天然酶和突變酶的酶學性質進行了分析，結果表明突變酶的ph穩定性和熱穩定性均優于天然酶，更適合啤酒發酵。 -
Because the catalytic site of a - aldc has not been certified and the catalytic mechanism has to be also further proved, it is difficult to enhance the enzyme activity in low temperature by the rational design
由於- aldc的活性部位尚未完全確定，催化機理也需進一步證實，在這種條件下通過酶的合理化設計來提高酶在低溫下的活力有一定困難。 -
The primary structure is unchanged in the evolved a - aldc, which implies that the catalytic mechanism of the evolved enzyme remained the same
但該酶的基本結構沒有改變，暗示該突變酶的催化機理與天然酶相同。 -
Therefore, we selected the method of directed evolution to modify the a - aldc gene in order to obtain the mutants with high enzyme activity in low temperature
為此，我們採用定向進化的方法對酶基因進行改造，以期獲得更適合於啤酒發酵的表達- aldc的工程菌株。 -
However, the normal temperature of fermentation is 10 c and the optimum temperature of a - aldc is 30 - 40 c, which indicted that the activity of a - aldc in the normal temperature of fermentation is 15 - 20 % of that in the optimum temperature
但是在啤酒生產中，正常的發酵溫度約為10 ，而該酶的最適溫度為30 40 ，在正常發酵溫度下，酶活力只能達到最高活力的15 20 。
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