antisense dna 中文意思是什麼
antisense dna
解釋
反義dna-
Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed
本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。 -
Construction of an expressing vector for antisense rna - ribozyme chimeric dna sequence against tomato acc synthase gene and transformation of tomato
核酶嵌合基因植物表達載體的構建及對番茄的轉化 -
Part i effects of gnt - v on cell proliferation, cell sensitivity to egf and the egf receptor of h7721 cell line using mtt method, it was found that the proliferation of cells transfected with sense gnt - v cdna was facilitated, and both of the total 3h - tdr incorporation and the specific incorporation per cell were also increased. oppositely, these parameters were reduced in cells transfected with antisense cdna of gnt - v. these results suggested that cell proliferation and dna synthesis were modulated by gnt - v
第一部分gnt - v對h7721細胞生長、 egf敏感性和egf受體的影響用mtt方法發現轉染正義gnt - vcdna的h7721細胞增殖速度加快，而且無論是~ 3h - tdr的總參入量或每個細胞的參入量均見增加，說明dna合成增強，而轉染反義gnt - vcdna的h7721細胞則完全相反， dna合成和細胞增殖速度均見降低，這提示gnt - v可調節細胞的生長。 -
Antisense nucleic acid technique is a method using single - stranded complementary sequences with specificity assigned by watson - crick base - pairing targeted specific messenger rna or dna to block expression of target gene
反義核酸技術是根據堿基互補原理，使用與目標靶的遺傳物質（ mrna或dna ）特定互補的核酸片段封閉基因表達的技術方法。 -
After transformation, we have chosen the positive bacterium clones by bacterium colon pcr. with dna sequencing, we have made sure direction of dna sequence which inserted into the plasmid and have named sense and antisense reconstruction plas - mids respectively
採用sanger雙脫氧末端終止法再進一步進行dna序列測定分析，檢測doc一1rcdna序列插人表達載體的方向，確定正、反義重組體。 -
The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action, thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained. in this study, we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template. the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons
本研究設計以c tamrna為模板的反義cdna片段，從c ta基因5 』端第94位到500位核苷酸段設計引物，目的片段407bp ，覆蓋第116和188位兩個aug密碼子，也包含了第一外顯子和第二外顯子間的剪接位點：用常規分子生物學方法構建了反義片段的腺病毒表達載體（ padeasy - 1系統） ；腺病毒載體經hek293細胞包裝產生含反義片段的重組腺病毒，用氯化銫密度梯度離心法獲得純化的高滴度腺病毒；進行體外基因轉移，分別用反義片段真核表達載體轉染p388d1細胞和用重組腺病毒感染hela細胞，觀察導入的c ta基因反義rna抑制細胞內組成型或誘導型c ta基因表達的作用，從而達到調控mhc -類分子表達的目的。 -
Hsp70, a kind of molecular chaperone, has the main functions of taking part in protein folding, protein degredation and reparation of dna damadge and has important effects on the constructure and function of mitochondria. it has already been proved that there is a close correlation between hsp70 and the development of plants and animals. this paper deals with integrating sense and antisense cdnas of hsp70 into tobacco dna by constructing an expression vector of sense and antisense cdnas of hsp70 and gene - transforming methods - genegun bombarding and agrobacterium mediation. provided expression of hsp70 gene is inhibited by sense and antisense cdnas of hsp70 we can get male sterile plants so as to prove that antisense cdna of hsp70 leads to male sterility 1
Hsp70是一種分子伴侶，主要功能是參與細胞有關蛋白新生肽的折疊、亞基組裝、細胞內運輸、蛋白質降解及dna損傷的修復，對線粒體結構和功能發揮重要作用，已有研究證明hsp70與動植物的發育有密切的關系，本研究將hsp70正、反義cdna構建成表達載體，並運用基因槍和農桿菌介導法將hsp70正、反義cdna導入煙草，試通過hsp70反義cdna抑制hsp70基因的表達從而創造雄性不育株，以證明hsp70反義cdna能創造雄性不育系。
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